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The map label for this gene is gcvH2 [H]

Identifier: 134302509

GI number: 134302509

Start: 1577290

End: 1577673

Strand: Reverse

Name: gcvH2 [H]

Synonym: FTW_1665

Alternate gene names: 134302509

Gene position: 1577673-1577290 (Counterclockwise)

Preceding gene: 134302510

Following gene: 134302508

Centisome position: 83.1

GC content: 37.76

Gene sequence:

>384_bases
ATGTCAAATATTCCTAAAGAATTAAAGTATACAAAGTCGCATGAGTGGGTCAAAATAGATGGTGATGAAGTCACTGTAGG
TATTACAGCACATGCACAATCTTTATTGGGCGATTTAGTCTATGTTGAATTACCAGAAATAGGCAAAGAGTTCGCAGCTG
GTGATGATTCTTGTGTGGTTGAGTCTGTCAAAGCAGCCTCTGATGTATACGCTCCTGTTGATGGTGAGGTAATTGAAATT
AATGAAGCTTTGGTTGATGACCCTTCACGAGTCAATCATACACCATATAAAGATGGCTGGCTATTTAAACTTAAAATCTC
TGATGAATCTCAGTTAGCTGATTTGCTAACAGCTGATGCTTATGCAAAAACTCTTGAGGATTAA

Upstream 100 bases:

>100_bases
TAGTTAAACCTAAGTTTGTTAAAAATGGTAAATCTTTGATATAAAGTAAATAAGTATTTTTTAAAAAAACAATTAATCGT
AGTCAACACGGAGGTATAAA

Downstream 100 bases:

>100_bases
TTGCGTAAACACAAAAACTATTTTTAATCTTTTATAAAATCTTGTACAAAGACATGGAGGAAATATGTCTTTTATCCCGC
ATAAACTAGAACAAATCAAA

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MSNIPKELKYTKSHEWVKIDGDEVTVGITAHAQSLLGDLVYVELPEIGKEFAAGDDSCVVESVKAASDVYAPVDGEVIEI
NEALVDDPSRVNHTPYKDGWLFKLKISDESQLADLLTADAYAKTLED

Sequences:

>Translated_127_residues
MSNIPKELKYTKSHEWVKIDGDEVTVGITAHAQSLLGDLVYVELPEIGKEFAAGDDSCVVESVKAASDVYAPVDGEVIEI
NEALVDDPSRVNHTPYKDGWLFKLKISDESQLADLLTADAYAKTLED
>Mature_126_residues
SNIPKELKYTKSHEWVKIDGDEVTVGITAHAQSLLGDLVYVELPEIGKEFAAGDDSCVVESVKAASDVYAPVDGEVIEIN
EALVDDPSRVNHTPYKDGWLFKLKISDESQLADLLTADAYAKTLED

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=47.0588235294118, Blast_Score=120, Evalue=3e-28,
Organism=Homo sapiens, GI89057342, Length=115, Percent_Identity=47.8260869565217, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789271, Length=128, Percent_Identity=60.15625, Blast_Score=160, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17551294, Length=114, Percent_Identity=50, Blast_Score=123, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17507493, Length=118, Percent_Identity=46.6101694915254, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319272, Length=128, Percent_Identity=43.75, Blast_Score=110, Evalue=5e-26,
Organism=Drosophila melanogaster, GI17865652, Length=125, Percent_Identity=45.6, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13911; Mature: 13779

Theoretical pI: Translated: 4.08; Mature: 4.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIPKELKYTKSHEWVKIDGDEVTVGITAHAQSLLGDLVYVELPEIGKEFAAGDDSCVV
CCCCCCHHEECCCCCEEEECCCEEEEEEHHHHHHHHHCEEEEECCHHHHHHCCCCCHHHH
ESVKAASDVYAPVDGEVIEINEALVDDPSRVNHTPYKDGWLFKLKISDESQLADLLTADA
HHHHHHHHCCCCCCCCEEEECHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHH
YAKTLED
HHHHCCC
>Mature Secondary Structure 
SNIPKELKYTKSHEWVKIDGDEVTVGITAHAQSLLGDLVYVELPEIGKEFAAGDDSCVV
CCCCCHHEECCCCCEEEECCCEEEEEEHHHHHHHHHCEEEEECCHHHHHHCCCCCHHHH
ESVKAASDVYAPVDGEVIEINEALVDDPSRVNHTPYKDGWLFKLKISDESQLADLLTADA
HHHHHHHHCCCCCCCCEEEECHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHH
YAKTLED
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA