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The map label for this gene is murF [H]

Identifier: 134302495

GI number: 134302495

Start: 1554860

End: 1556218

Strand: Reverse

Name: murF [H]

Synonym: FTW_1651

Alternate gene names: 134302495

Gene position: 1556218-1554860 (Counterclockwise)

Preceding gene: 134302496

Following gene: 134302494

Centisome position: 81.97

GC content: 33.19

Gene sequence:

>1359_bases
ATGATAAAATCACTAAAACAATTAGCAATCCAAGCAGGCTTAGAATATTTGGGTGAAGATGTATCAATACAAACAGTAGC
TATAAATTCAAATGAAGTAAGACAAGATTGCTTATTTGTTGCAATTGTAGCTAATCGTGATGGACACGAGTTTATTCCAA
GTGCTATTGCTAATGGCGCTAAAGCTATTTTAGTTTCAAAAAAACAAGGTTTAGATATTCCACAAGTAGTTTGTGGCAAT
ACTATCAAAGGCTTACGGGCTTTAGCTAAAGAATATCGCAAATCACTAACTATGCCAATAATATCTTTAACTGGGAGTTG
CGGTAAAACATCTGTTAAAGAAATGATTGTAACTCTTCTTGGTGAGAGAAAAGTTCATTTTACTAAAGGTAACCTAAATA
ATTATCTTGGTGTACCAATGACAATATTAGAAACACCGCATGATGTCGATTTTGCAGTGATTGAGGCAGGTACAAATGTT
GGTGGTGAGATAAAAGCTGCAGCTGATATTATCCAACCAAATATAGCGATGATTACAAATGTTGGCGCTTGCCACCTTGA
GAACCTAAAAACGCTGGATGGTGTAATGATAGAAAAAGGGGAGCTTCTCAAAGCTTTGCCAAGAGATGGTTTTTGTATCG
TAAATCTAGATGATGAAAGAATTCCAAATTATGCTAAGCTGCTAGAGTGTAAAAAGATTAGCTGCTCAATGACTAATCCA
AAAGCTGATATTTTAGTATTAGATTATAGGGCAACATCAAATAGTTATGCTTTTAAGATTAGAATTTTTAGCCAAGAGTA
TGATTATCAACTACCAAACATTGGCAAGCATAATTTGTTTAATAGTGTCTTGGCTATTGCTAGTGTAGTTGCTGTGGGGC
TTGAGCCTAAGGATTTCTTACAAAATACACAAAATATCAAAAACTATAAAGGTAGATTTTCAACTGAAAAACTCAGTGAT
AAACTAACTCTTATAGATGATACATATAATGCAAGTGCTGGCGCTGTGCAAGCTGCAATTGAAGATTTGGCAGAATTTGA
TGGTAAAAAAATATTGGCTATTAGTTCAATGCGTGAGCTAGGAGATGAAGCTGAAAATTATCATCGAAAAATGGGGCAAT
GGCTTAAAAAGGCAAATTTAGATAAGATATTTTTATTTGGTGAGAAAAATTTAATAGATTATGTTTTGGCAGAATATTCA
AACCAGAATGCTAAATATTATGAATCTAAAGACGAGCTAAATAATGATTTAGCTAAAATATTGGATGAATATAAGTCACA
AAGTACTAAGCTAACAGTCAAAGGGGCTAGAAGTTTTAAGATGGAAGAAGTTGTCAAATTTGTTAAGGAAAACTTATAA

Upstream 100 bases:

>100_bases
AAACAATAAGAGCAAATAGATGCTAGAAAACTATAATAGCTAGCCTTTAGTTATTGATTATCTTAGAATATATTTAACTT
AACTATATGAGTTTTTATTA

Downstream 100 bases:

>100_bases
GGGACATTATGAAAAGACTTTTTGCAATATTAGTTTTTGTATTACTTGTAAGCGTATCTTTTGGTAAAGATATCATAAAG
TATCCATTAATTGAGTCAAA

Product: UDP-N--acetylmuramoylalanyl-D-glutamyl-2,6- diaminopimelate-D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MIKSLKQLAIQAGLEYLGEDVSIQTVAINSNEVRQDCLFVAIVANRDGHEFIPSAIANGAKAILVSKKQGLDIPQVVCGN
TIKGLRALAKEYRKSLTMPIISLTGSCGKTSVKEMIVTLLGERKVHFTKGNLNNYLGVPMTILETPHDVDFAVIEAGTNV
GGEIKAAADIIQPNIAMITNVGACHLENLKTLDGVMIEKGELLKALPRDGFCIVNLDDERIPNYAKLLECKKISCSMTNP
KADILVLDYRATSNSYAFKIRIFSQEYDYQLPNIGKHNLFNSVLAIASVVAVGLEPKDFLQNTQNIKNYKGRFSTEKLSD
KLTLIDDTYNASAGAVQAAIEDLAEFDGKKILAISSMRELGDEAENYHRKMGQWLKKANLDKIFLFGEKNLIDYVLAEYS
NQNAKYYESKDELNNDLAKILDEYKSQSTKLTVKGARSFKMEEVVKFVKENL

Sequences:

>Translated_452_residues
MIKSLKQLAIQAGLEYLGEDVSIQTVAINSNEVRQDCLFVAIVANRDGHEFIPSAIANGAKAILVSKKQGLDIPQVVCGN
TIKGLRALAKEYRKSLTMPIISLTGSCGKTSVKEMIVTLLGERKVHFTKGNLNNYLGVPMTILETPHDVDFAVIEAGTNV
GGEIKAAADIIQPNIAMITNVGACHLENLKTLDGVMIEKGELLKALPRDGFCIVNLDDERIPNYAKLLECKKISCSMTNP
KADILVLDYRATSNSYAFKIRIFSQEYDYQLPNIGKHNLFNSVLAIASVVAVGLEPKDFLQNTQNIKNYKGRFSTEKLSD
KLTLIDDTYNASAGAVQAAIEDLAEFDGKKILAISSMRELGDEAENYHRKMGQWLKKANLDKIFLFGEKNLIDYVLAEYS
NQNAKYYESKDELNNDLAKILDEYKSQSTKLTVKGARSFKMEEVVKFVKENL
>Mature_452_residues
MIKSLKQLAIQAGLEYLGEDVSIQTVAINSNEVRQDCLFVAIVANRDGHEFIPSAIANGAKAILVSKKQGLDIPQVVCGN
TIKGLRALAKEYRKSLTMPIISLTGSCGKTSVKEMIVTLLGERKVHFTKGNLNNYLGVPMTILETPHDVDFAVIEAGTNV
GGEIKAAADIIQPNIAMITNVGACHLENLKTLDGVMIEKGELLKALPRDGFCIVNLDDERIPNYAKLLECKKISCSMTNP
KADILVLDYRATSNSYAFKIRIFSQEYDYQLPNIGKHNLFNSVLAIASVVAVGLEPKDFLQNTQNIKNYKGRFSTEKLSD
KLTLIDDTYNASAGAVQAAIEDLAEFDGKKILAISSMRELGDEAENYHRKMGQWLKKANLDKIFLFGEKNLIDYVLAEYS
NQNAKYYESKDELNNDLAKILDEYKSQSTKLTVKGARSFKMEEVVKFVKENL

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=451, Percent_Identity=33.7028824833703, Blast_Score=238, Evalue=6e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50093; Mature: 50093

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSLKQLAIQAGLEYLGEDVSIQTVAINSNEVRQDCLFVAIVANRDGHEFIPSAIANGA
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCHHCCCEEEEEEEECCCCHHHHHHHHCCCC
KAILVSKKQGLDIPQVVCGNTIKGLRALAKEYRKSLTMPIISLTGSCGKTSVKEMIVTLL
EEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHH
GERKVHFTKGNLNNYLGVPMTILETPHDVDFAVIEAGTNVGGEIKAAADIIQPNIAMITN
CCCEEEEECCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHCCCHHEEEC
VGACHLENLKTLDGVMIEKGELLKALPRDGFCIVNLDDERIPNYAKLLECKKISCSMTNP
CCHHHHCCCHHHCCEEECCCHHHHHCCCCCEEEEECCHHHCCCHHHHHHHHHCEEECCCC
KADILVLDYRATSNSYAFKIRIFSQEYDYQLPNIGKHNLFNSVLAIASVVAVGLEPKDFL
CCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
QNTQNIKNYKGRFSTEKLSDKLTLIDDTYNASAGAVQAAIEDLAEFDGKKILAISSMREL
HHHHHHHHHCCCCCHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHH
GDEAENYHRKMGQWLKKANLDKIFLFGEKNLIDYVLAEYSNQNAKYYESKDELNNDLAKI
HHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCEECCHHHHHHHHHHH
LDEYKSQSTKLTVKGARSFKMEEVVKFVKENL
HHHHCCCCCEEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MIKSLKQLAIQAGLEYLGEDVSIQTVAINSNEVRQDCLFVAIVANRDGHEFIPSAIANGA
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCHHCCCEEEEEEEECCCCHHHHHHHHCCCC
KAILVSKKQGLDIPQVVCGNTIKGLRALAKEYRKSLTMPIISLTGSCGKTSVKEMIVTLL
EEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHH
GERKVHFTKGNLNNYLGVPMTILETPHDVDFAVIEAGTNVGGEIKAAADIIQPNIAMITN
CCCEEEEECCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHCCCHHEEEC
VGACHLENLKTLDGVMIEKGELLKALPRDGFCIVNLDDERIPNYAKLLECKKISCSMTNP
CCHHHHCCCHHHCCEEECCCHHHHHCCCCCEEEEECCHHHCCCHHHHHHHHHCEEECCCC
KADILVLDYRATSNSYAFKIRIFSQEYDYQLPNIGKHNLFNSVLAIASVVAVGLEPKDFL
CCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
QNTQNIKNYKGRFSTEKLSDKLTLIDDTYNASAGAVQAAIEDLAEFDGKKILAISSMREL
HHHHHHHHHCCCCCHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHH
GDEAENYHRKMGQWLKKANLDKIFLFGEKNLIDYVLAEYSNQNAKYYESKDELNNDLAKI
HHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCEECCHHHHHHHHHHH
LDEYKSQSTKLTVKGARSFKMEEVVKFVKENL
HHHHCCCCCEEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]