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The map label for this gene is pckA [H]

Identifier: 134302473

GI number: 134302473

Start: 1525435

End: 1527027

Strand: Reverse

Name: pckA [H]

Synonym: FTW_1621

Alternate gene names: 134302473

Gene position: 1527027-1525435 (Counterclockwise)

Preceding gene: 134302476

Following gene: 134302472

Centisome position: 80.43

GC content: 32.33

Gene sequence:

>1593_bases
TTGTTAATGGATCCTTCAACCATAGATGAGTATAAGTATCTATATACTTGTGAAAATGTGCATAAAAATCTCTCTCTCCA
AGAATTAGCAAAATACGCACATAATAGTGATGGTTTTATTTGCTACGATAATAAAACTTTAGTAGTTGACAGTGGTGAAA
TCAAGGGTCGACTTCCTGATGATAAATATATTGTTGAGACTAAATATGCCAAAAAAAATATTTGGTGGAGTGAAAATGGC
TCAGATAATAAAAGATTAAAAAGAAAAAACTGGAAGCTGATTAAACAAAGATTATGTCAAGAGGTAGCAACAAAGGATCT
TTTTGTTGTAGATGGTTTTTATAATCATGATGAGAGATATACCATAGCAGTTAGACTAATAACTACCCAAGCTTCAGCTG
CATATTTTTTTAAGTTGATATCGATAACTCCGAGTGAAAAAGAATTACAGTCATTTGAGCCACAATGGGTAATAATGCAT
TCCCCTCAAACACAGATAGAAGATTACCAAGACCTTGATTTAAATTCTTCAAAAGTAATAGCAACTAACCTTAAGCAGCG
AGAAAGTATATTGGTTGGGACTATGTATCTTGCTGAGATAAATAAGGTATTGTTGTCGATTATGAGCTATTATCTCTTGC
TCAACGATATTGGCGTATTTTACTGTGCTGTCAGTGTTGATGCTGAAGCTAATAGTACAATATTTTTTGGTTTGTCAGGT
AGCGGTAAAACAACTTTAGCTTTAGACCAAAATAAGAGTCTTGTTGCAAATGAGGCTATTGCTTGGACAGAAATGCGTGG
TGTGTATAGTCTAGAGTCAGGTTTGACAATTAAATCAGCAAGTTTTAAAAAAGACGATCCGCGTATCAAACAAGCATTAG
CTGGGGACCTTTTGATAGAAAATCCTAACTTTGATGATAGTCACAATATTATATTTGGTGAGAAAAATAGTTCACAAAGT
AATACTTATGTAACTTTCCCACGTGAAAATTTTGTTAATGTTATAAACACTGATAACCCTAACACAATAATTTTTCTTGT
CAAAGATGCAAAAGGTGTATTACCAAGAGTTGCAAAACTTAGTAAAGGTCAGGCAATTTACTATTTTTTATCTGGATATA
CTTCTACATCTATAGGAGTAGAGGCAGGTGTTACAGAGCCTAAACCAGAGTTTACAAGTTGCTATGCTCAGCCATTTCTA
TTACTTAAGCCAACTAGATATGCAAGTATATTGCGTCAGCGTTTGAAGCATAGTAATGCTAAAATTTATATGATAAATGT
TGGCTGGATTGAGGGTGATTATAAGACAGGTAGAAGGGTTCCTGTTGAAGAGACAAAACTTATTGTTAACTACTTATTGA
CTAAACCAGAGAATGTTAGCTTTAAATTTACACGTCAAAAATATTTTAACTTTAAAGCTTTAACTTCAATAAATGATAAT
GGTCAAGAGCTTCAACTTACCAATAATTGGAGTGATAGTGCTGAATATAAAAAAGAATATAAGTCTTTAGCAAGAGCTTT
TATCAAAAACTATGAGCAGTTTGAAAATGATGATTTTGCACTAAAATATAAAAAATTTGAACCAATTATTTAG

Upstream 100 bases:

>100_bases
TACTTTAATAAAAAAACAATAACATATTTTTTTGAAAAAAAAGTATATACTAGCTATAATTTTTACTTAAAATCTAATTG
CTCTATAAGCTAAGGGACTT

Downstream 100 bases:

>100_bases
ACGCTAATTTTGACTCTATTAAAAAAATAACATATCTATTATAATACTCCAAGGTCATTAAACATTTTAAATATATGCTG
ATTTATCTTTTTGAATGGTT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MLMDPSTIDEYKYLYTCENVHKNLSLQELAKYAHNSDGFICYDNKTLVVDSGEIKGRLPDDKYIVETKYAKKNIWWSENG
SDNKRLKRKNWKLIKQRLCQEVATKDLFVVDGFYNHDERYTIAVRLITTQASAAYFFKLISITPSEKELQSFEPQWVIMH
SPQTQIEDYQDLDLNSSKVIATNLKQRESILVGTMYLAEINKVLLSIMSYYLLLNDIGVFYCAVSVDAEANSTIFFGLSG
SGKTTLALDQNKSLVANEAIAWTEMRGVYSLESGLTIKSASFKKDDPRIKQALAGDLLIENPNFDDSHNIIFGEKNSSQS
NTYVTFPRENFVNVINTDNPNTIIFLVKDAKGVLPRVAKLSKGQAIYYFLSGYTSTSIGVEAGVTEPKPEFTSCYAQPFL
LLKPTRYASILRQRLKHSNAKIYMINVGWIEGDYKTGRRVPVEETKLIVNYLLTKPENVSFKFTRQKYFNFKALTSINDN
GQELQLTNNWSDSAEYKKEYKSLARAFIKNYEQFENDDFALKYKKFEPII

Sequences:

>Translated_530_residues
MLMDPSTIDEYKYLYTCENVHKNLSLQELAKYAHNSDGFICYDNKTLVVDSGEIKGRLPDDKYIVETKYAKKNIWWSENG
SDNKRLKRKNWKLIKQRLCQEVATKDLFVVDGFYNHDERYTIAVRLITTQASAAYFFKLISITPSEKELQSFEPQWVIMH
SPQTQIEDYQDLDLNSSKVIATNLKQRESILVGTMYLAEINKVLLSIMSYYLLLNDIGVFYCAVSVDAEANSTIFFGLSG
SGKTTLALDQNKSLVANEAIAWTEMRGVYSLESGLTIKSASFKKDDPRIKQALAGDLLIENPNFDDSHNIIFGEKNSSQS
NTYVTFPRENFVNVINTDNPNTIIFLVKDAKGVLPRVAKLSKGQAIYYFLSGYTSTSIGVEAGVTEPKPEFTSCYAQPFL
LLKPTRYASILRQRLKHSNAKIYMINVGWIEGDYKTGRRVPVEETKLIVNYLLTKPENVSFKFTRQKYFNFKALTSINDN
GQELQLTNNWSDSAEYKKEYKSLARAFIKNYEQFENDDFALKYKKFEPII
>Mature_530_residues
MLMDPSTIDEYKYLYTCENVHKNLSLQELAKYAHNSDGFICYDNKTLVVDSGEIKGRLPDDKYIVETKYAKKNIWWSENG
SDNKRLKRKNWKLIKQRLCQEVATKDLFVVDGFYNHDERYTIAVRLITTQASAAYFFKLISITPSEKELQSFEPQWVIMH
SPQTQIEDYQDLDLNSSKVIATNLKQRESILVGTMYLAEINKVLLSIMSYYLLLNDIGVFYCAVSVDAEANSTIFFGLSG
SGKTTLALDQNKSLVANEAIAWTEMRGVYSLESGLTIKSASFKKDDPRIKQALAGDLLIENPNFDDSHNIIFGEKNSSQS
NTYVTFPRENFVNVINTDNPNTIIFLVKDAKGVLPRVAKLSKGQAIYYFLSGYTSTSIGVEAGVTEPKPEFTSCYAQPFL
LLKPTRYASILRQRLKHSNAKIYMINVGWIEGDYKTGRRVPVEETKLIVNYLLTKPENVSFKFTRQKYFNFKALTSINDN
GQELQLTNNWSDSAEYKKEYKSLARAFIKNYEQFENDDFALKYKKFEPII

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=479, Percent_Identity=32.776617954071, Blast_Score=291, Evalue=5e-80,
Organism=Saccharomyces cerevisiae, GI6322950, Length=529, Percent_Identity=31.1909262759924, Blast_Score=265, Evalue=2e-71,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 60752; Mature: 60752

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMDPSTIDEYKYLYTCENVHKNLSLQELAKYAHNSDGFICYDNKTLVVDSGEIKGRLPD
CCCCCCCCCCCEEEEEEHHHHCCCCHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCCC
DKYIVETKYAKKNIWWSENGSDNKRLKRKNWKLIKQRLCQEVATKDLFVVDGFYNHDERY
CCEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECEECCCCEE
TIAVRLITTQASAAYFFKLISITPSEKELQSFEPQWVIMHSPQTQIEDYQDLDLNSSKVI
EEEEEEEEECCCCEEEEEEEECCCCHHHHHCCCCCEEEEECCCHHCCCCCCCCCCCCEEE
ATNLKQRESILVGTMYLAEINKVLLSIMSYYLLLNDIGVFYCAVSVDAEANSTIFFGLSG
EECCHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEECC
SGKTTLALDQNKSLVANEAIAWTEMRGVYSLESGLTIKSASFKKDDPRIKQALAGDLLIE
CCCEEEEECCCCCEECCCCEEEHHHCCEEEECCCCEEEECCCCCCCHHHHHHHCCCEEEE
NPNFDDSHNIIFGEKNSSQSNTYVTFPRENFVNVINTDNPNTIIFLVKDAKGVLPRVAKL
CCCCCCCCCEEEECCCCCCCCEEEEECCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHC
SKGQAIYYFLSGYTSTSIGVEAGVTEPKPEFTSCYAQPFLLLKPTRYASILRQRLKHSNA
CCCCEEEEEEECCCCCCCCEECCCCCCCCHHHHHHCCCEEEECCHHHHHHHHHHHHCCCC
KIYMINVGWIEGDYKTGRRVPVEETKLIVNYLLTKPENVSFKFTRQKYFNFKALTSINDN
EEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHCCEEEEEECCCC
GQELQLTNNWSDSAEYKKEYKSLARAFIKNYEQFENDDFALKYKKFEPII
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MLMDPSTIDEYKYLYTCENVHKNLSLQELAKYAHNSDGFICYDNKTLVVDSGEIKGRLPD
CCCCCCCCCCCEEEEEEHHHHCCCCHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCCC
DKYIVETKYAKKNIWWSENGSDNKRLKRKNWKLIKQRLCQEVATKDLFVVDGFYNHDERY
CCEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECEECCCCEE
TIAVRLITTQASAAYFFKLISITPSEKELQSFEPQWVIMHSPQTQIEDYQDLDLNSSKVI
EEEEEEEEECCCCEEEEEEEECCCCHHHHHCCCCCEEEEECCCHHCCCCCCCCCCCCEEE
ATNLKQRESILVGTMYLAEINKVLLSIMSYYLLLNDIGVFYCAVSVDAEANSTIFFGLSG
EECCHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEECC
SGKTTLALDQNKSLVANEAIAWTEMRGVYSLESGLTIKSASFKKDDPRIKQALAGDLLIE
CCCEEEEECCCCCEECCCCEEEHHHCCEEEECCCCEEEECCCCCCCHHHHHHHCCCEEEE
NPNFDDSHNIIFGEKNSSQSNTYVTFPRENFVNVINTDNPNTIIFLVKDAKGVLPRVAKL
CCCCCCCCCEEEECCCCCCCCEEEEECCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHC
SKGQAIYYFLSGYTSTSIGVEAGVTEPKPEFTSCYAQPFLLLKPTRYASILRQRLKHSNA
CCCCEEEEEEECCCCCCCCEECCCCCCCCHHHHHHCCCEEEECCHHHHHHHHHHHHCCCC
KIYMINVGWIEGDYKTGRRVPVEETKLIVNYLLTKPENVSFKFTRQKYFNFKALTSINDN
EEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHCCEEEEEECCCC
GQELQLTNNWSDSAEYKKEYKSLARAFIKNYEQFENDDFALKYKKFEPII
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA