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The map label for this gene is dus2 [H]

Identifier: 134302420

GI number: 134302420

Start: 1463130

End: 1464113

Strand: Direct

Name: dus2 [H]

Synonym: FTW_1557

Alternate gene names: 134302420

Gene position: 1463130-1464113 (Clockwise)

Preceding gene: 134302419

Following gene: 134302424

Centisome position: 77.07

GC content: 32.32

Gene sequence:

>984_bases
ATGAAAATAACAACTAGAAAAATTTGTATAGCACCAATGCTTGACTGGACTGATAGGCACTATCGTTACATGATGCGTCT
AATCACCAGGCATACAATGTTATATACTGAAATGGTTACTTTAAACGCAATCTTACATGGTAATAAAGAATTTTTGCTTA
AATATAATCCTCAAGAAAATCCTGTGACACTACAGTTAGGTGGTTGCGTTCCAAAAGACTTTATCAGTTGTGGCAAGCTT
GCCGAAGAACTTGGCTATAATGAAATTAACATAAATGTTGGCTGCCCAAGTGAAAGAGTTAAAAAAGGTAATTTTGGCTT
ATCATTAATGGCAGAGCCAGAGCTTGTAGCAGACTGTGTCAAAGCTATGAAAGATAACCTAAATATCCCAATTTCAGTTA
AATGTCGTATTGGCTATGATCATAATGATAGCTATGAAGAACTATATAATTTTGTTGATAAGCAAGTTCAAGCAGGAATA
GATTACCTTTGTATTCATGCACGTAAAGGTTGGCTTAGCGGTCTATCACCAAAAGAAAATCGTACCATACCTGAGCTTAA
ATATGATGTTGTCTACAGTATCAAAAAAGATTTCCCAAACCTTGAACTAGGGATTAATGGCGGTATTACAACAATTCAAG
AAATTCAACAGCACCTTAAATATGTGGATAGTGTTATGATAGGTAGAGAAGCATATCATAACCCAATGTTATTTAAAGAT
TTTGATAATATTTTCTATAATCAACCTACTACTAATATATCAGCATTAGAAATTGCTTATAAGATGGCTGAATATATAAA
AGACCAAATGGATAATGATCCATATATAAAACTTAACAATATAACTAAACATACCTTAAACCTATTTAATGGTTTGCCTA
ATGCTAAAGTATATCGTCGTTATCTAAGCGAAAATGCCACAAAAAAAGAAGCCAATGTAGATACCTTTTTAACAGCTTTA
GAAGTTTTTGAGAATAAATTATAG

Upstream 100 bases:

>100_bases
TGAGAAATATCGTCCACAAATTGATAAAATCATTCGCCGCTTAACTTTTGATGATGATCCTTTATAATTTTATAACAACT
TAAATCTCGTAGCTTTTAAA

Downstream 100 bases:

>100_bases
TCTAATTTACTTGATATTATTAAGCTGTGCTTGAATATTAGTAGCAACCCATTTTGCTGCTTGTTGATAATTATCTGTAA
TAGCATTAGTCTGTTTGTCT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKITTRKICIAPMLDWTDRHYRYMMRLITRHTMLYTEMVTLNAILHGNKEFLLKYNPQENPVTLQLGGCVPKDFISCGKL
AEELGYNEININVGCPSERVKKGNFGLSLMAEPELVADCVKAMKDNLNIPISVKCRIGYDHNDSYEELYNFVDKQVQAGI
DYLCIHARKGWLSGLSPKENRTIPELKYDVVYSIKKDFPNLELGINGGITTIQEIQQHLKYVDSVMIGREAYHNPMLFKD
FDNIFYNQPTTNISALEIAYKMAEYIKDQMDNDPYIKLNNITKHTLNLFNGLPNAKVYRRYLSENATKKEANVDTFLTAL
EVFENKL

Sequences:

>Translated_327_residues
MKITTRKICIAPMLDWTDRHYRYMMRLITRHTMLYTEMVTLNAILHGNKEFLLKYNPQENPVTLQLGGCVPKDFISCGKL
AEELGYNEININVGCPSERVKKGNFGLSLMAEPELVADCVKAMKDNLNIPISVKCRIGYDHNDSYEELYNFVDKQVQAGI
DYLCIHARKGWLSGLSPKENRTIPELKYDVVYSIKKDFPNLELGINGGITTIQEIQQHLKYVDSVMIGREAYHNPMLFKD
FDNIFYNQPTTNISALEIAYKMAEYIKDQMDNDPYIKLNNITKHTLNLFNGLPNAKVYRRYLSENATKKEANVDTFLTAL
EVFENKL
>Mature_327_residues
MKITTRKICIAPMLDWTDRHYRYMMRLITRHTMLYTEMVTLNAILHGNKEFLLKYNPQENPVTLQLGGCVPKDFISCGKL
AEELGYNEININVGCPSERVKKGNFGLSLMAEPELVADCVKAMKDNLNIPISVKCRIGYDHNDSYEELYNFVDKQVQAGI
DYLCIHARKGWLSGLSPKENRTIPELKYDVVYSIKKDFPNLELGINGGITTIQEIQQHLKYVDSVMIGREAYHNPMLFKD
FDNIFYNQPTTNISALEIAYKMAEYIKDQMDNDPYIKLNNITKHTLNLFNGLPNAKVYRRYLSENATKKEANVDTFLTAL
EVFENKL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=242, Percent_Identity=25.6198347107438, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI31742496, Length=245, Percent_Identity=24.4897959183673, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI145693211, Length=321, Percent_Identity=49.8442367601246, Blast_Score=338, Evalue=2e-94,
Organism=Escherichia coli, GI1788462, Length=255, Percent_Identity=27.843137254902, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789660, Length=243, Percent_Identity=25.5144032921811, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17507177, Length=268, Percent_Identity=24.2537313432836, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=240, Percent_Identity=23.3333333333333, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24580595, Length=238, Percent_Identity=24.7899159663866, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920448, Length=238, Percent_Identity=24.7899159663866, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37705; Mature: 37705

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITTRKICIAPMLDWTDRHYRYMMRLITRHTMLYTEMVTLNAILHGNKEFLLKYNPQEN
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
PVTLQLGGCVPKDFISCGKLAEELGYNEININVGCPSERVKKGNFGLSLMAEPELVADCV
CEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEEECHHHHHHHH
KAMKDNLNIPISVKCRIGYDHNDSYEELYNFVDKQVQAGIDYLCIHARKGWLSGLSPKEN
HHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCC
RTIPELKYDVVYSIKKDFPNLELGINGGITTIQEIQQHLKYVDSVMIGREAYHNPMLFKD
CCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FDNIFYNQPTTNISALEIAYKMAEYIKDQMDNDPYIKLNNITKHTLNLFNGLPNAKVYRR
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCHHHHHH
YLSENATKKEANVDTFLTALEVFENKL
HHHCCCCCHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKITTRKICIAPMLDWTDRHYRYMMRLITRHTMLYTEMVTLNAILHGNKEFLLKYNPQEN
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
PVTLQLGGCVPKDFISCGKLAEELGYNEININVGCPSERVKKGNFGLSLMAEPELVADCV
CEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEEECHHHHHHHH
KAMKDNLNIPISVKCRIGYDHNDSYEELYNFVDKQVQAGIDYLCIHARKGWLSGLSPKEN
HHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCC
RTIPELKYDVVYSIKKDFPNLELGINGGITTIQEIQQHLKYVDSVMIGREAYHNPMLFKD
CCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FDNIFYNQPTTNISALEIAYKMAEYIKDQMDNDPYIKLNNITKHTLNLFNGLPNAKVYRR
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCHHHHHH
YLSENATKKEANVDTFLTALEVFENKL
HHHCCCCCHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]