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The map label for this gene is gyrB [H]

Identifier: 134302418

GI number: 134302418

Start: 1459703

End: 1462114

Strand: Reverse

Name: gyrB [H]

Synonym: FTW_1555

Alternate gene names: 134302418

Gene position: 1462114-1459703 (Counterclockwise)

Preceding gene: 134302421

Following gene: 134302417

Centisome position: 77.02

GC content: 34.62

Gene sequence:

>2412_bases
ATGTCTGAGAATAAAGCTTATGACTCATCGAGTATTAAGGTACTTAAAGGTTTAGATGCTGTTAGAAAAAGACCTGGGAT
GTATATTGGTGATACCGATGATGGTAGCGGCTTACATCATATGGTATTTGAGGTTGTTGATAATGCTATCGATGAAGCCT
TAGCTGGTTATTGTGATGATATTAAAGTTATTATAAATAAGGATGGCTCTGTATCTGTATCAGATAATGGTAGAGGTATT
CCTACAGATATTCATAAAGAAGAAGGGCGCTCTGCAGCAGAGGTTATTTTGACAGTATTGCATGCAGGTGGTAAGTTTGA
TGATAACTCTTATAAAGTATCAGGCGGTTTACATGGTGTTGGTGTATCAGTTGTAAATGCGCTTTCAGAACAATTAAAGT
TAACAATTTATAGAAATGGTATCGAGCACTATCAAGAGTATGTTCATGGAGTACCACAGTTTCCATTAAAAGAAGTTGGT
TCAACAGATAAGACCGGAACACTAATTACTTTTAAACCGAGTAAAGAAACGTTTTCATTTGTTGACTTCGATTATGATAT
CTTGATGAAGAGAATTCGAGAGTTATCTTTCTTAAACTCTGGCGTCAAAATTGAACTTATTGATAAAATTAATAATAGGT
CAGAAATATTTAAGTATGATGGTGGTATAGTTGCTTTTGTTAAGTATTTGAACAATAGCAAAAAACCTATTCATGAAAAT
GTTATAGCTGTAAATGGTATCAAAGATGATATTCAAGTAGAGCTTGCTTTGCAATGGAATGACTCATATAAAGAATCAAT
TTTTTGCTTTACAAATAATATCCCACAAAGAGATGGTGGTACGCATTTATCTGGCCTTAAAGCAGCTGTTACTCGTACGA
TGAATAATTATATTGAATCAGAAGGTTTAAATAAAAAGCTAAAAGTTACTTTAACAGGTGAAGATACGCGCGAAGGTTTA
GCTGCAGTTTTATCTGTGAAAGTTCCTGATCCAAAATTCTCATCACAAACTAAAGATAAACTAGTATCATCTGATGTTAA
ATCAGCTGTAGAGTCATTAGTTAATGAAAAACTACAAGAGTTTCTTTTAGAAAACCCTAAAGAAGCTAAAATTATATGTG
AGAAAATTTTAGATTCAGCAAAAGCTCGTGAAGCTGCTCGTAAAGCCCGTGATATGACGCGTCGTAAGGGTGCTTTGGAT
ATTGCTGGCCTACCTGGTAAGTTAGCAGATTGCCAAGAAAAAGATCCGGCATTGTCTGAAATTTATCTTGTTGAGGGAGA
CTCTGCAGGAGGTTCAGCAAAGCAGGCTCGTGATCGTAAAACGCAGGCGATTTTGCCACTAAAAGGTAAGATTCTCAATG
TTGAGAAAGCACGTTTTGATAAAATGCTTGGTTCTCAAGAGGTAGCTACGCTCATTAAAGCTCTAGGTTGTGGTATAGGT
GCGGAAGATTATAACCCTGATAAAACTAGATATCACAAAATTATCCTAATGACGGATGCTGATGTTGATGGCTCGCATAT
TAGAACACTTCTTTTGACATTTTTCTATAGACAAATGCCAGAGCTTGTTGAGAGAGGGTATTTATATATTGCTCAACCAC
CTTTATATAAGGTAAAAAAAGGTAAGCAAGAAACATATCTAAAAGATGAAGATGCTTTGGCAGAATATCTTGGTAATATA
GGTCTAGAAGGCGCGTGTATCTATTTAAGTAATGGCAATGTTATTTCTGGTCAAGTTTTGGCTAATTATTATGAGCTTTA
TCAGAAGTCACAAAAAGTAATTAAAAAATATACAAAAACGTATCCTGAGAAGCTTCTTAGAGTTATGGCATATGGAACTA
AGTATGTTGATGAAAGTGCGGATATCTCTGAGTGGTGGCAGAAAATTGTTGAAAATTGTAATCAAAAGGCTTTAGCATAT
GAGCGCTTTAAGCTTATAGAAACAAAAGATATAGGTGAGGATGGTAAAGAGAATATATCATATGGAGTTAATCATTATAT
TAATGGTTATGATACTGACTATATTGTTAAGAGTAGTTTCTTTAGTACTAAAGATTATGAAGATCTAGTTACATATGGAG
ATGTTTTATCGGATATATCTTTTGAAGGAGCTTATGTAGAGAGAGGTGCTAAAAAAGAATATGTTGATGATTTTGAATCA
GCAATTGATTGGTTGCTTAAAGAAGCTAGAAAAGGTAATGATGTACAGCGTTATAAAGGTCTAGGTGAGATGAATCCTGG
TCAGCTTTGGGAAACTACTATGGATCCTGATAATAGAGTTCTTCTACAAGTATCAATAAAAGATGCGGTTGAAGCTGATG
CTTTATTTACTACTCTGATGGGTGATGAGGTTGAGCCACGCAGAAACTTTATTGAGTCAAATGCTCTTAATGTGGTTAAC
TTAGACGTTTAA

Upstream 100 bases:

>100_bases
TTAACTCACATTGTTATCAACATTAATAATCAAGATTTATTTGTGTTATAATACTTAATGATTGAATTTTAAAATATAAC
GATAAATTTGGAGCTTGTTA

Downstream 100 bases:

>100_bases
TTACATCTAACTTTTTAAAATTATATTATATCATTTTTAAATCTTAACAAAAATTAACTTATCTATAGCACCTAAAAAAA
TTATAATCCTTTGAGTTGGT

Product: DNA gyrase, B subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 803; Mature: 802

Protein sequence:

>803_residues
MSENKAYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNAIDEALAGYCDDIKVIINKDGSVSVSDNGRGI
PTDIHKEEGRSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEQLKLTIYRNGIEHYQEYVHGVPQFPLKEVG
STDKTGTLITFKPSKETFSFVDFDYDILMKRIRELSFLNSGVKIELIDKINNRSEIFKYDGGIVAFVKYLNNSKKPIHEN
VIAVNGIKDDIQVELALQWNDSYKESIFCFTNNIPQRDGGTHLSGLKAAVTRTMNNYIESEGLNKKLKVTLTGEDTREGL
AAVLSVKVPDPKFSSQTKDKLVSSDVKSAVESLVNEKLQEFLLENPKEAKIICEKILDSAKAREAARKARDMTRRKGALD
IAGLPGKLADCQEKDPALSEIYLVEGDSAGGSAKQARDRKTQAILPLKGKILNVEKARFDKMLGSQEVATLIKALGCGIG
AEDYNPDKTRYHKIILMTDADVDGSHIRTLLLTFFYRQMPELVERGYLYIAQPPLYKVKKGKQETYLKDEDALAEYLGNI
GLEGACIYLSNGNVISGQVLANYYELYQKSQKVIKKYTKTYPEKLLRVMAYGTKYVDESADISEWWQKIVENCNQKALAY
ERFKLIETKDIGEDGKENISYGVNHYINGYDTDYIVKSSFFSTKDYEDLVTYGDVLSDISFEGAYVERGAKKEYVDDFES
AIDWLLKEARKGNDVQRYKGLGEMNPGQLWETTMDPDNRVLLQVSIKDAVEADALFTTLMGDEVEPRRNFIESNALNVVN
LDV

Sequences:

>Translated_803_residues
MSENKAYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNAIDEALAGYCDDIKVIINKDGSVSVSDNGRGI
PTDIHKEEGRSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEQLKLTIYRNGIEHYQEYVHGVPQFPLKEVG
STDKTGTLITFKPSKETFSFVDFDYDILMKRIRELSFLNSGVKIELIDKINNRSEIFKYDGGIVAFVKYLNNSKKPIHEN
VIAVNGIKDDIQVELALQWNDSYKESIFCFTNNIPQRDGGTHLSGLKAAVTRTMNNYIESEGLNKKLKVTLTGEDTREGL
AAVLSVKVPDPKFSSQTKDKLVSSDVKSAVESLVNEKLQEFLLENPKEAKIICEKILDSAKAREAARKARDMTRRKGALD
IAGLPGKLADCQEKDPALSEIYLVEGDSAGGSAKQARDRKTQAILPLKGKILNVEKARFDKMLGSQEVATLIKALGCGIG
AEDYNPDKTRYHKIILMTDADVDGSHIRTLLLTFFYRQMPELVERGYLYIAQPPLYKVKKGKQETYLKDEDALAEYLGNI
GLEGACIYLSNGNVISGQVLANYYELYQKSQKVIKKYTKTYPEKLLRVMAYGTKYVDESADISEWWQKIVENCNQKALAY
ERFKLIETKDIGEDGKENISYGVNHYINGYDTDYIVKSSFFSTKDYEDLVTYGDVLSDISFEGAYVERGAKKEYVDDFES
AIDWLLKEARKGNDVQRYKGLGEMNPGQLWETTMDPDNRVLLQVSIKDAVEADALFTTLMGDEVEPRRNFIESNALNVVN
LDV
>Mature_802_residues
SENKAYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNAIDEALAGYCDDIKVIINKDGSVSVSDNGRGIP
TDIHKEEGRSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEQLKLTIYRNGIEHYQEYVHGVPQFPLKEVGS
TDKTGTLITFKPSKETFSFVDFDYDILMKRIRELSFLNSGVKIELIDKINNRSEIFKYDGGIVAFVKYLNNSKKPIHENV
IAVNGIKDDIQVELALQWNDSYKESIFCFTNNIPQRDGGTHLSGLKAAVTRTMNNYIESEGLNKKLKVTLTGEDTREGLA
AVLSVKVPDPKFSSQTKDKLVSSDVKSAVESLVNEKLQEFLLENPKEAKIICEKILDSAKAREAARKARDMTRRKGALDI
AGLPGKLADCQEKDPALSEIYLVEGDSAGGSAKQARDRKTQAILPLKGKILNVEKARFDKMLGSQEVATLIKALGCGIGA
EDYNPDKTRYHKIILMTDADVDGSHIRTLLLTFFYRQMPELVERGYLYIAQPPLYKVKKGKQETYLKDEDALAEYLGNIG
LEGACIYLSNGNVISGQVLANYYELYQKSQKVIKKYTKTYPEKLLRVMAYGTKYVDESADISEWWQKIVENCNQKALAYE
RFKLIETKDIGEDGKENISYGVNHYINGYDTDYIVKSSFFSTKDYEDLVTYGDVLSDISFEGAYVERGAKKEYVDDFESA
IDWLLKEARKGNDVQRYKGLGEMNPGQLWETTMDPDNRVLLQVSIKDAVEADALFTTLMGDEVEPRRNFIESNALNVVNL
DV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=652, Percent_Identity=25.4601226993865, Blast_Score=130, Evalue=6e-30,
Organism=Homo sapiens, GI19913406, Length=557, Percent_Identity=26.5709156193896, Blast_Score=129, Evalue=1e-29,
Organism=Escherichia coli, GI48994957, Length=807, Percent_Identity=63.3209417596035, Blast_Score=1023, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=551, Percent_Identity=39.9274047186933, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17535065, Length=632, Percent_Identity=26.2658227848101, Blast_Score=145, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=516, Percent_Identity=24.6124031007752, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=155, Percent_Identity=33.5483870967742, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324241, Length=585, Percent_Identity=24.7863247863248, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17136538, Length=531, Percent_Identity=26.9303201506591, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89756; Mature: 89625

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00154 ATPASE_E1_E2 ; PS00177 TOPOISOMERASE_II ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENKAYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNAIDEALAGYCDD
CCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
IKVIINKDGSVSVSDNGRGIPTDIHKEEGRSAAEVILTVLHAGGKFDDNSYKVSGGLHGV
EEEEEECCCCEEECCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHH
GVSVVNALSEQLKLTIYRNGIEHYQEYVHGVPQFPLKEVGSTDKTGTLITFKPSKETFSF
HHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEECCCCCCCEE
VDFDYDILMKRIRELSFLNSGVKIELIDKINNRSEIFKYDGGIVAFVKYLNNSKKPIHEN
ECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHCCCCCCCHHC
VIAVNGIKDDIQVELALQWNDSYKESIFCFTNNIPQRDGGTHLSGLKAAVTRTMNNYIES
EEEECCCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EGLNKKLKVTLTGEDTREGLAAVLSVKVPDPKFSSQTKDKLVSSDVKSAVESLVNEKLQE
CCCCCEEEEEECCCCHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLLENPKEAKIICEKILDSAKAREAARKARDMTRRKGALDIAGLPGKLADCQEKDPALSE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEE
IYLVEGDSAGGSAKQARDRKTQAILPLKGKILNVEKARFDKMLGSQEVATLIKALGCGIG
EEEEECCCCCCCHHHHHHHHHCEECCCCCCEECHHHHHHHHHHCHHHHHHHHHHHHCCCC
AEDYNPDKTRYHKIILMTDADVDGSHIRTLLLTFFYRQMPELVERGYLYIAQPPLYKVKK
CCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHC
GKQETYLKDEDALAEYLGNIGLEGACIYLSNGNVISGQVLANYYELYQKSQKVIKKYTKT
CCCHHCCCCHHHHHHHHHCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
YPEKLLRVMAYGTKYVDESADISEWWQKIVENCNQKALAYERFKLIETKDIGEDGKENIS
HHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCCCHHHHH
YGVNHYINGYDTDYIVKSSFFSTKDYEDLVTYGDVLSDISFEGAYVERGAKKEYVDDFES
HCHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHH
AIDWLLKEARKGNDVQRYKGLGEMNPGQLWETTMDPDNRVLLQVSIKDAVEADALFTTLM
HHHHHHHHHHCCCHHHHHCCCCCCCCCCCEECCCCCCCEEEEEEEHHHHHHHHHHHHHHH
GDEVEPRRNFIESNALNVVNLDV
CCCCCHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
SENKAYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNAIDEALAGYCDD
CCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
IKVIINKDGSVSVSDNGRGIPTDIHKEEGRSAAEVILTVLHAGGKFDDNSYKVSGGLHGV
EEEEEECCCCEEECCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHH
GVSVVNALSEQLKLTIYRNGIEHYQEYVHGVPQFPLKEVGSTDKTGTLITFKPSKETFSF
HHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEECCCCCCCEE
VDFDYDILMKRIRELSFLNSGVKIELIDKINNRSEIFKYDGGIVAFVKYLNNSKKPIHEN
ECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHCCCCCCCHHC
VIAVNGIKDDIQVELALQWNDSYKESIFCFTNNIPQRDGGTHLSGLKAAVTRTMNNYIES
EEEECCCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EGLNKKLKVTLTGEDTREGLAAVLSVKVPDPKFSSQTKDKLVSSDVKSAVESLVNEKLQE
CCCCCEEEEEECCCCHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLLENPKEAKIICEKILDSAKAREAARKARDMTRRKGALDIAGLPGKLADCQEKDPALSE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEE
IYLVEGDSAGGSAKQARDRKTQAILPLKGKILNVEKARFDKMLGSQEVATLIKALGCGIG
EEEEECCCCCCCHHHHHHHHHCEECCCCCCEECHHHHHHHHHHCHHHHHHHHHHHHCCCC
AEDYNPDKTRYHKIILMTDADVDGSHIRTLLLTFFYRQMPELVERGYLYIAQPPLYKVKK
CCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHC
GKQETYLKDEDALAEYLGNIGLEGACIYLSNGNVISGQVLANYYELYQKSQKVIKKYTKT
CCCHHCCCCHHHHHHHHHCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
YPEKLLRVMAYGTKYVDESADISEWWQKIVENCNQKALAYERFKLIETKDIGEDGKENIS
HHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCCCHHHHH
YGVNHYINGYDTDYIVKSSFFSTKDYEDLVTYGDVLSDISFEGAYVERGAKKEYVDDFES
HCHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHH
AIDWLLKEARKGNDVQRYKGLGEMNPGQLWETTMDPDNRVLLQVSIKDAVEADALFTTLM
HHHHHHHHHHCCCHHHHHCCCCCCCCCCCEECCCCCCCEEEEEEEHHHHHHHHHHHHHHH
GDEVEPRRNFIESNALNVVNLDV
CCCCCHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]