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The map label for this gene is pnp [H]

Identifier: 134302408

GI number: 134302408

Start: 1448725

End: 1450806

Strand: Reverse

Name: pnp [H]

Synonym: FTW_1544

Alternate gene names: 134302408

Gene position: 1450806-1448725 (Counterclockwise)

Preceding gene: 134302409

Following gene: 134302407

Centisome position: 76.42

GC content: 36.12

Gene sequence:

>2082_bases
GTGAAAATATTTAGAGAAGTTTTTGAACTAGGTAACAAAGAAATAATTCTTGAAACAGGTGGTATGGCACGTCAAGCAGA
TGGGTCAGTGACTGTAAGTTGTGGGAATAATGTTGTATTGGTGACAACAGTAGTTAAAAAATCAGTAGCTGATGGTACTG
ACTTTTTTCCGTTATCTGTGCATTATTTAGAGAAAACTTACGCTGCTGGTAAAATACCGGGTGGTTTCTTAAGAAGAGAG
GGAAGACCTTCAGAAGAACAAATTCTTATTTCTAGGCTAATAGACAGATCTATAAGACCATCATTTCCAGATGGGTTTTT
TAATGAAATTCAAATCGTTGCTACAGTTTTATCTTATGATGGTGCTGTTGCACCTGATATACTAGCTTTAATAGGTGCTT
CGGCATCTTTAGCAATATCTGGAGCACCTTACGATGATGTTGTTGCTGGTGTAAGAGTGGGTTATACAAATGGTAAGTAT
ATTCTTAATCCAAATAAACAGGATTTGAGAGATTCAGATCTAGATCTAGTAGTCTCTGGAACAGATGATGCTATTTTAAT
GGTTGAATCTGAAGCTAATAGCCTTCCAGAATCTGTTATGCTTGGTGGTATCTTATATGCGCACAAGCATTTAAAAACTA
TTATTAACTCGATTAATAGACTTGCTAAAGTAGCATCTAAACCTCGTATAGAATATTCTATATATCAAATCAACAAGTTT
CTTAAATCACAAATTAAGTCACAATTTTTTGGTGAAATTAAGAATGCCTATACTATAGCGTCAAAGCAAGAAAGAAACTT
AAAACTTAATGCAATTAGAAAAAATGTTCTTGAGTATATTTTCTCTAGTGATGTTGATGGTAACGAGTATACTGAAAAAG
AAATATTAGAAGCTTTCCACGATATTGAAAAAGATCTTGTAAGATCAAATATTCTTGAGGGTAAACCAAGAATAGATGGT
AGATGTACAGAGACTATCCGACCTATCAATGTCAAAATAGGTGTTTTACCGGGGGTACATGGATCTGCTTTGTTTACACG
TGGTGAGACTCAAGCTTTAGTTGTAACTACTTTAGGTAGTGATAGAGATGCACAGCTTGTGGAATCTTTAGATGGCATAG
AGAAATGTCGTTATATGTTGCATTATAACTTTCCACCATATTCTGTGGGAGAGTGTGGTATGGTTGGTATGGCTCCAAAG
CGTCGCGAGATTGGTCATGCTAACTTAGCTAAGCGCGCAACTCAAGCAGTATTCCCTAATGAAGAAGCATACCCGTATGT
TGTTAGAGTTGTCTCTGAAATTCTAGAGTCAAATGGTTCAAGCTCTATGGCGACAGTATGTGGTTCTTCTTTATCAATGA
TGGATGCTGGTGTACCAATTGCTGAGCCAGTAGCAGGTATTGCTATGGGACTTATTAAAGATGGTGCAAAATATGCTGTG
TTATCAGATATTCTAGGCGATGAAGATCATTTGGGTGATATGGATTTTAAAGTTGCTGGTACTAGATATGGTGTTACAGC
ATTACAGATGGATATTAAAATCAAAGGCATCTCAAGAGAAATTCTTGAGCAAGCATTAGAGCAGGCAAGAGTAGGTAGAT
TACATATTCTAGGAATCATGAATGAAGTAATAAAAGAGCATAAAGAAGCTGTATCTGATGTGGCGCCACAAATTCATGTG
ATGAATATAAATCCTGCTAAAATTAAAGATGTCGTTGGTCGAGGAGGTGCTACTGTAAAAGGTATAGTTGAGAAGACTGG
TGCACAAATTGATACTAGTGATTCTGGTGAAGTTAAGGTTTTTGCTAAAGACAAAAAATCTATGGATATGGCTGTAGCAA
TGATAGAAGAAATCGTTGCAGAAGTGGAAGAAGGGCAAGTTTACAAGGGCAAAATAGTTAAGCTTTTAGATTCTGGAGTA
TTTGTTAATCTTCTTGGTAGTCAAGATGGATATTTACCATTCTCTGAAATTGAACAAGCTGGCATGAAAACTAACTCGTT
AGTAGAAGGTCAAGGCTTAGAAGTTTTAGTTCAAAATATCGATAGAGGTGGTAGAGTTAAACTTTCTCTTGTAGCGAGGT
AA

Upstream 100 bases:

>100_bases
AAAGATACCGTAGTCTAATCAAGAAATTAAATATACGTAGATAATAAACTTCATCTTAATCGTTCTTACATCATTAAATT
CAAAAAAATAAGGTTAAACT

Downstream 100 bases:

>100_bases
TTATTATGAGTGAAGTAGTTCAAAGCGTTGATCCGATAAACTTTACAGAAGCCGCTTCTTTAAAGGTTAAAGAACTAATT
GAAGAGGAGGGTGATAATTC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MKIFREVFELGNKEIILETGGMARQADGSVTVSCGNNVVLVTTVVKKSVADGTDFFPLSVHYLEKTYAAGKIPGGFLRRE
GRPSEEQILISRLIDRSIRPSFPDGFFNEIQIVATVLSYDGAVAPDILALIGASASLAISGAPYDDVVAGVRVGYTNGKY
ILNPNKQDLRDSDLDLVVSGTDDAILMVESEANSLPESVMLGGILYAHKHLKTIINSINRLAKVASKPRIEYSIYQINKF
LKSQIKSQFFGEIKNAYTIASKQERNLKLNAIRKNVLEYIFSSDVDGNEYTEKEILEAFHDIEKDLVRSNILEGKPRIDG
RCTETIRPINVKIGVLPGVHGSALFTRGETQALVVTTLGSDRDAQLVESLDGIEKCRYMLHYNFPPYSVGECGMVGMAPK
RREIGHANLAKRATQAVFPNEEAYPYVVRVVSEILESNGSSSMATVCGSSLSMMDAGVPIAEPVAGIAMGLIKDGAKYAV
LSDILGDEDHLGDMDFKVAGTRYGVTALQMDIKIKGISREILEQALEQARVGRLHILGIMNEVIKEHKEAVSDVAPQIHV
MNINPAKIKDVVGRGGATVKGIVEKTGAQIDTSDSGEVKVFAKDKKSMDMAVAMIEEIVAEVEEGQVYKGKIVKLLDSGV
FVNLLGSQDGYLPFSEIEQAGMKTNSLVEGQGLEVLVQNIDRGGRVKLSLVAR

Sequences:

>Translated_693_residues
MKIFREVFELGNKEIILETGGMARQADGSVTVSCGNNVVLVTTVVKKSVADGTDFFPLSVHYLEKTYAAGKIPGGFLRRE
GRPSEEQILISRLIDRSIRPSFPDGFFNEIQIVATVLSYDGAVAPDILALIGASASLAISGAPYDDVVAGVRVGYTNGKY
ILNPNKQDLRDSDLDLVVSGTDDAILMVESEANSLPESVMLGGILYAHKHLKTIINSINRLAKVASKPRIEYSIYQINKF
LKSQIKSQFFGEIKNAYTIASKQERNLKLNAIRKNVLEYIFSSDVDGNEYTEKEILEAFHDIEKDLVRSNILEGKPRIDG
RCTETIRPINVKIGVLPGVHGSALFTRGETQALVVTTLGSDRDAQLVESLDGIEKCRYMLHYNFPPYSVGECGMVGMAPK
RREIGHANLAKRATQAVFPNEEAYPYVVRVVSEILESNGSSSMATVCGSSLSMMDAGVPIAEPVAGIAMGLIKDGAKYAV
LSDILGDEDHLGDMDFKVAGTRYGVTALQMDIKIKGISREILEQALEQARVGRLHILGIMNEVIKEHKEAVSDVAPQIHV
MNINPAKIKDVVGRGGATVKGIVEKTGAQIDTSDSGEVKVFAKDKKSMDMAVAMIEEIVAEVEEGQVYKGKIVKLLDSGV
FVNLLGSQDGYLPFSEIEQAGMKTNSLVEGQGLEVLVQNIDRGGRVKLSLVAR
>Mature_693_residues
MKIFREVFELGNKEIILETGGMARQADGSVTVSCGNNVVLVTTVVKKSVADGTDFFPLSVHYLEKTYAAGKIPGGFLRRE
GRPSEEQILISRLIDRSIRPSFPDGFFNEIQIVATVLSYDGAVAPDILALIGASASLAISGAPYDDVVAGVRVGYTNGKY
ILNPNKQDLRDSDLDLVVSGTDDAILMVESEANSLPESVMLGGILYAHKHLKTIINSINRLAKVASKPRIEYSIYQINKF
LKSQIKSQFFGEIKNAYTIASKQERNLKLNAIRKNVLEYIFSSDVDGNEYTEKEILEAFHDIEKDLVRSNILEGKPRIDG
RCTETIRPINVKIGVLPGVHGSALFTRGETQALVVTTLGSDRDAQLVESLDGIEKCRYMLHYNFPPYSVGECGMVGMAPK
RREIGHANLAKRATQAVFPNEEAYPYVVRVVSEILESNGSSSMATVCGSSLSMMDAGVPIAEPVAGIAMGLIKDGAKYAV
LSDILGDEDHLGDMDFKVAGTRYGVTALQMDIKIKGISREILEQALEQARVGRLHILGIMNEVIKEHKEAVSDVAPQIHV
MNINPAKIKDVVGRGGATVKGIVEKTGAQIDTSDSGEVKVFAKDKKSMDMAVAMIEEIVAEVEEGQVYKGKIVKLLDSGV
FVNLLGSQDGYLPFSEIEQAGMKTNSLVEGQGLEVLVQNIDRGGRVKLSLVAR

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=709, Percent_Identity=36.5303244005642, Blast_Score=415, Evalue=1e-116,
Organism=Escherichia coli, GI145693187, Length=685, Percent_Identity=52.2627737226277, Blast_Score=730, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=660, Percent_Identity=34.0909090909091, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI281362905, Length=658, Percent_Identity=37.3860182370821, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651641, Length=658, Percent_Identity=37.3860182370821, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651643, Length=658, Percent_Identity=37.3860182370821, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI161079377, Length=605, Percent_Identity=37.6859504132231, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 75361; Mature: 75361

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1 ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFREVFELGNKEIILETGGMARQADGSVTVSCGNNVVLVTTVVKKSVADGTDFFPLSV
CCHHHHHHHCCCCEEEEECCCCCEECCCCEEEECCCCEEEEEEHHHHHHCCCCCCCEEHH
HYLEKTYAAGKIPGGFLRREGRPSEEQILISRLIDRSIRPSFPDGFFNEIQIVATVLSYD
HHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
GAVAPDILALIGASASLAISGAPYDDVVAGVRVGYTNGKYILNPNKQDLRDSDLDLVVSG
CCCHHHHHHHHCCCCEEEECCCCHHHHHHCEEEEECCCEEEECCCHHHCCCCCCEEEEEC
TDDAILMVESEANSLPESVMLGGILYAHKHLKTIINSINRLAKVASKPRIEYSIYQINKF
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHH
LKSQIKSQFFGEIKNAYTIASKQERNLKLNAIRKNVLEYIFSSDVDGNEYTEKEILEAFH
HHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
DIEKDLVRSNILEGKPRIDGRCTETIRPINVKIGVLPGVHGSALFTRGETQALVVTTLGS
HHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEEEEECCCCCCCEEEECCCCCEEEEEECCC
DRDAQLVESLDGIEKCRYMLHYNFPPYSVGECGMVGMAPKRREIGHANLAKRATQAVFPN
CCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCC
EEAYPYVVRVVSEILESNGSSSMATVCGSSLSMMDAGVPIAEPVAGIAMGLIKDGAKYAV
CCCCHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHH
LSDILGDEDHLGDMDFKVAGTRYGVTALQMDIKIKGISREILEQALEQARVGRLHILGIM
HHHHCCCCCCCCCCCEEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHH
NEVIKEHKEAVSDVAPQIHVMNINPAKIKDVVGRGGATVKGIVEKTGAQIDTSDSGEVKV
HHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHCCCEECCCCCCCEEE
FAKDKKSMDMAVAMIEEIVAEVEEGQVYKGKIVKLLDSGVFVNLLGSQDGYLPFSEIEQA
EECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHC
GMKTNSLVEGQGLEVLVQNIDRGGRVKLSLVAR
CCCCCCEECCCCHHHHHHHCCCCCEEEEEEECC
>Mature Secondary Structure
MKIFREVFELGNKEIILETGGMARQADGSVTVSCGNNVVLVTTVVKKSVADGTDFFPLSV
CCHHHHHHHCCCCEEEEECCCCCEECCCCEEEECCCCEEEEEEHHHHHHCCCCCCCEEHH
HYLEKTYAAGKIPGGFLRREGRPSEEQILISRLIDRSIRPSFPDGFFNEIQIVATVLSYD
HHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
GAVAPDILALIGASASLAISGAPYDDVVAGVRVGYTNGKYILNPNKQDLRDSDLDLVVSG
CCCHHHHHHHHCCCCEEEECCCCHHHHHHCEEEEECCCEEEECCCHHHCCCCCCEEEEEC
TDDAILMVESEANSLPESVMLGGILYAHKHLKTIINSINRLAKVASKPRIEYSIYQINKF
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHH
LKSQIKSQFFGEIKNAYTIASKQERNLKLNAIRKNVLEYIFSSDVDGNEYTEKEILEAFH
HHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
DIEKDLVRSNILEGKPRIDGRCTETIRPINVKIGVLPGVHGSALFTRGETQALVVTTLGS
HHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEEEEECCCCCCCEEEECCCCCEEEEEECCC
DRDAQLVESLDGIEKCRYMLHYNFPPYSVGECGMVGMAPKRREIGHANLAKRATQAVFPN
CCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCC
EEAYPYVVRVVSEILESNGSSSMATVCGSSLSMMDAGVPIAEPVAGIAMGLIKDGAKYAV
CCCCHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHH
LSDILGDEDHLGDMDFKVAGTRYGVTALQMDIKIKGISREILEQALEQARVGRLHILGIM
HHHHCCCCCCCCCCCEEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHH
NEVIKEHKEAVSDVAPQIHVMNINPAKIKDVVGRGGATVKGIVEKTGAQIDTSDSGEVKV
HHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHCCCEECCCCCCCEEE
FAKDKKSMDMAVAMIEEIVAEVEEGQVYKGKIVKLLDSGVFVNLLGSQDGYLPFSEIEQA
EECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHC
GMKTNSLVEGQGLEVLVQNIDRGGRVKLSLVAR
CCCCCCEECCCCHHHHHHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA