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The map label for this gene is rumA [H]

Identifier: 134302402

GI number: 134302402

Start: 1444349

End: 1445698

Strand: Reverse

Name: rumA [H]

Synonym: FTW_1538

Alternate gene names: 134302402

Gene position: 1445698-1444349 (Counterclockwise)

Preceding gene: 134302403

Following gene: 134302680

Centisome position: 76.15

GC content: 33.26

Gene sequence:

>1350_bases
ATGGGAAGATCTAGACATCATAATAAGCTAAAAGAGGGAATCTTTGAGGCTGAAATTACAGCATTAAGTCATGATGGTAG
AGGTATTGCAAAAGTTGATGGTAAAACTACTTTTATACCTTTCACATTACCTGGCGAAGTAGTTAAATTTGAATATACAT
TCACAAAAGCAAAATTCGATGAAGCAAAAGTAGTTGAGTATGTTAAAAAATCATCAAATAGAGTTAATCCGCCTTGTGAC
CATTTTCAAATATGTGGAGGTTGTAGCTTACAACATATGTCTACAGATGCACAGATCGAGCATAAGCAACAAACTTTAAT
TAATCAATTAAAATATATTGGTAATGGTGTTGAGCCAGAGAATATTTTACCACCACTACGCACGAGTAATACTGAAGGAT
ATCGTAATAAAGCACGTTTGGGTGTTAGGTATGTCAGTAAAAAAGGTAAAATTCTTGTTGGCTTTAGAGAGCGTAATGGT
AGGTTCTTGGCAGATATAGATAAGTGTATCGTTTTAAATCCCCTTGTCGGTGACAAAATAACAGAAATATCTTCATTTAT
AGAGACGTTATCTATATATCAACATATAGCACAATTAGAAATAGCAATAGATGATACAAGACCTGCTATGATTGTGCGTC
ATCTAGAACCATTTACAAATGAAGACTTAGAAAAGCTAAGATCATTTGCACAAGAAAATAATTATTGGATATATTTACAA
TCAAAAGGTCCAGATACTATTTTTAGATTATACCCACAAGGTGATGTAGAGCCTAAAAAGCTAAGCTATCAGCCTGCTGC
TGGAATAGATATTGGTTTTGAACCTAATGATTTTACTCAGGTTAATAATGATATCAATAAAAAAATGATTAAAAGAGCGA
TTGAGCTTTTAGATATATCAGAAAATGATTCTATTATTGACTTATTCTGTGGTTTAGGTAACTTTACGCTGCCAATTTCT
CAGCATGCAAAAACTGTAATTGGCGTGGAGGGTGAGCTAACAATGGTCAAAAGAGCCAAAGAAACTGCTGATAATAATAA
CATAACAAATGTTAATTTTTATGCGGCAAATCTTTTTGAGAGTTTTGAGGATAAAGAGTGGTTTAATAATTTTGAATATA
ACAAGATGCTTTTAGATCCGCCTCGAGCAGGCGCACAAGAAGTTTGTAACAATATTGAAAAATTTAATGTTAAGCGCATA
GTTTATGTTTCATGTGATACTGCTGCTTTAGCACGTGATGCTGGAATTTTGGTCAATACCAAAGGTTATAAACTAATAAG
TGCTGGTGTAATGGATATGTTCCCTCATACAATGCATGTTGAGTCAATAGCTGTTTTTGAGAAGATCTAG

Upstream 100 bases:

>100_bases
AATCTAAAATAGTAAAAGCTAAGACTAAGTTTTGTGATTAAGTTGTATTTATGATTTGAATTAAATACAATTGTCGTAAT
ATTTTTTTGTTTAATTATTT

Downstream 100 bases:

>100_bases
GAGACATTTATGTACATAGGTTTAGATATTGGCGGTAGTAATATCTCTGCTGGTATTTTTGATGAGCAAAAGAACCTTAT
TAAAACTGCAAAGGTTAAAT

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MGRSRHHNKLKEGIFEAEITALSHDGRGIAKVDGKTTFIPFTLPGEVVKFEYTFTKAKFDEAKVVEYVKKSSNRVNPPCD
HFQICGGCSLQHMSTDAQIEHKQQTLINQLKYIGNGVEPENILPPLRTSNTEGYRNKARLGVRYVSKKGKILVGFRERNG
RFLADIDKCIVLNPLVGDKITEISSFIETLSIYQHIAQLEIAIDDTRPAMIVRHLEPFTNEDLEKLRSFAQENNYWIYLQ
SKGPDTIFRLYPQGDVEPKKLSYQPAAGIDIGFEPNDFTQVNNDINKKMIKRAIELLDISENDSIIDLFCGLGNFTLPIS
QHAKTVIGVEGELTMVKRAKETADNNNITNVNFYAANLFESFEDKEWFNNFEYNKMLLDPPRAGAQEVCNNIEKFNVKRI
VYVSCDTAALARDAGILVNTKGYKLISAGVMDMFPHTMHVESIAVFEKI

Sequences:

>Translated_449_residues
MGRSRHHNKLKEGIFEAEITALSHDGRGIAKVDGKTTFIPFTLPGEVVKFEYTFTKAKFDEAKVVEYVKKSSNRVNPPCD
HFQICGGCSLQHMSTDAQIEHKQQTLINQLKYIGNGVEPENILPPLRTSNTEGYRNKARLGVRYVSKKGKILVGFRERNG
RFLADIDKCIVLNPLVGDKITEISSFIETLSIYQHIAQLEIAIDDTRPAMIVRHLEPFTNEDLEKLRSFAQENNYWIYLQ
SKGPDTIFRLYPQGDVEPKKLSYQPAAGIDIGFEPNDFTQVNNDINKKMIKRAIELLDISENDSIIDLFCGLGNFTLPIS
QHAKTVIGVEGELTMVKRAKETADNNNITNVNFYAANLFESFEDKEWFNNFEYNKMLLDPPRAGAQEVCNNIEKFNVKRI
VYVSCDTAALARDAGILVNTKGYKLISAGVMDMFPHTMHVESIAVFEKI
>Mature_448_residues
GRSRHHNKLKEGIFEAEITALSHDGRGIAKVDGKTTFIPFTLPGEVVKFEYTFTKAKFDEAKVVEYVKKSSNRVNPPCDH
FQICGGCSLQHMSTDAQIEHKQQTLINQLKYIGNGVEPENILPPLRTSNTEGYRNKARLGVRYVSKKGKILVGFRERNGR
FLADIDKCIVLNPLVGDKITEISSFIETLSIYQHIAQLEIAIDDTRPAMIVRHLEPFTNEDLEKLRSFAQENNYWIYLQS
KGPDTIFRLYPQGDVEPKKLSYQPAAGIDIGFEPNDFTQVNNDINKKMIKRAIELLDISENDSIIDLFCGLGNFTLPISQ
HAKTVIGVEGELTMVKRAKETADNNNITNVNFYAANLFESFEDKEWFNNFEYNKMLLDPPRAGAQEVCNNIEKFNVKRIV
YVSCDTAALARDAGILVNTKGYKLISAGVMDMFPHTMHVESIAVFEKI

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=191, Percent_Identity=27.2251308900524, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI51173878, Length=191, Percent_Identity=27.2251308900524, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1789148, Length=436, Percent_Identity=35.7798165137615, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1787083, Length=184, Percent_Identity=24.4565217391304, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790403, Length=235, Percent_Identity=26.8085106382979, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322909, Length=431, Percent_Identity=24.8259860788863, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24666579, Length=197, Percent_Identity=31.4720812182741, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 50828; Mature: 50696

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRSRHHNKLKEGIFEAEITALSHDGRGIAKVDGKTTFIPFTLPGEVVKFEYTFTKAKFD
CCCCCHHHHHHHCHHHHEEEEEECCCCEEEEECCCEEEEEEECCCCEEEEEEEEECCCCC
EAKVVEYVKKSSNRVNPPCDHFQICGGCSLQHMSTDAQIEHKQQTLINQLKYIGNGVEPE
HHHHHHHHHHCCCCCCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCH
NILPPLRTSNTEGYRNKARLGVRYVSKKGKILVGFRERNGRFLADIDKCIVLNPLVGDKI
HCCCCCCCCCCCCCCHHHHHCEEEECCCCCEEEEEECCCCCEEEECCCEEEECCCCCCHH
TEISSFIETLSIYQHIAQLEIAIDDTRPAMIVRHLEPFTNEDLEKLRSFAQENNYWIYLQ
HHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEE
SKGPDTIFRLYPQGDVEPKKLSYQPAAGIDIGFEPNDFTQVNNDINKKMIKRAIELLDIS
CCCCCEEEEECCCCCCCHHHCCCCCCCCEECCCCCCCHHHHCCHHHHHHHHHHHHHHCCC
ENDSIIDLFCGLGNFTLPISQHAKTVIGVEGELTMVKRAKETADNNNITNVNFYAANLFE
CCCCEEEEEECCCCCEEEHHHCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHH
SFEDKEWFNNFEYNKMLLDPPRAGAQEVCNNIEKFNVKRIVYVSCDTAALARDAGILVNT
HHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHCCCCEEEEC
KGYKLISAGVMDMFPHTMHVESIAVFEKI
CCCEEEECCHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
GRSRHHNKLKEGIFEAEITALSHDGRGIAKVDGKTTFIPFTLPGEVVKFEYTFTKAKFD
CCCCHHHHHHHCHHHHEEEEEECCCCEEEEECCCEEEEEEECCCCEEEEEEEEECCCCC
EAKVVEYVKKSSNRVNPPCDHFQICGGCSLQHMSTDAQIEHKQQTLINQLKYIGNGVEPE
HHHHHHHHHHCCCCCCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCH
NILPPLRTSNTEGYRNKARLGVRYVSKKGKILVGFRERNGRFLADIDKCIVLNPLVGDKI
HCCCCCCCCCCCCCCHHHHHCEEEECCCCCEEEEEECCCCCEEEECCCEEEECCCCCCHH
TEISSFIETLSIYQHIAQLEIAIDDTRPAMIVRHLEPFTNEDLEKLRSFAQENNYWIYLQ
HHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEE
SKGPDTIFRLYPQGDVEPKKLSYQPAAGIDIGFEPNDFTQVNNDINKKMIKRAIELLDIS
CCCCCEEEEECCCCCCCHHHCCCCCCCCEECCCCCCCHHHHCCHHHHHHHHHHHHHHCCC
ENDSIIDLFCGLGNFTLPISQHAKTVIGVEGELTMVKRAKETADNNNITNVNFYAANLFE
CCCCEEEEEECCCCCEEEHHHCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHH
SFEDKEWFNNFEYNKMLLDPPRAGAQEVCNNIEKFNVKRIVYVSCDTAALARDAGILVNT
HHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHCCCCEEEEC
KGYKLISAGVMDMFPHTMHVESIAVFEKI
CCCEEEECCHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA