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The map label for this gene is eno [H]

Identifier: 134302399

GI number: 134302399

Start: 1438839

End: 1440209

Strand: Reverse

Name: eno [H]

Synonym: FTW_1532

Alternate gene names: 134302399

Gene position: 1440209-1438839 (Counterclockwise)

Preceding gene: 134302400

Following gene: 134302398

Centisome position: 75.86

GC content: 36.03

Gene sequence:

>1371_bases
ATGTCGTCACAAATAAAACAAGTTTTTGCCAGACAGATATTAGATTCGCGTGGTAATCCTACAGTTGAAGTAGATGTGGT
TTTGGAAAGTGGTGCTTTTGGTCGTGCTGCTGTACCTTCTGGTGCTTCTACCGGAATTAGAGAAGCTCTAGAGTTAAGAG
ATGGTAACAAAGCCCTTTTTCTAGGTAAGAGTGTATATAAAGCTGTTGAGAATGTTAATACTAAGATAGCTCAAGCAGTC
AAAGGTTTAGATGCATTAGATCAAAGGTTAATTGATAAGACTATGATTGAACTAGATGGTTCTGAGAATAAGAAAAATCT
AGGTGCAAATGCAATTTTAGGTGTTTCACTAGCTACTGCTAGAGCTGCTGCATCACATCTTAGAAAACCTTTTTACCGTT
ATCTAATGGATGTCAAAGAATATCTAATGCCAGTACCAATGATGAATGTTATTAATGGCGGTTCACATGCTGATAATAAT
GTTGATATGCAAGAATTTATGATTGTTCCAGCTGGTTTTGATACTTTTTCAGAAGCTCTAAGATGTGGTACAGAAGTTTT
CCACATACTTAAAAAGGTTCTAATTGCTGATGGTTACAGTGTCGCTGGTGTTGGTGATGAGGGCGGTTATGCTCCTGATC
TACCGTCAAATGAGGCGGCTATAGAGGCAATATTAAAAGCAGTTAAAGAAGCAGGTTATGAGCCTGGTAAACATGTATTT
ATAGCTTTAGATCCTGCAAGTAGTGAGTTTTATAAAGATGGTAAGTACGAACTTAAGTCAGAGAATAAGTCATTAACAAG
TGAAGAAATGATTGATTATTATGCTGCTTGGGTTGAGAAGTATCCTATAGTATCTATAGAAGATGGACTTGCAGAAGAAG
ATTGGGCTGGTTGGAAACTTTTAACTGAAAAACTTGGTAACAAGGTACAGTTAGTCGGTGATGATTTATTTGTTACTAAT
CCAAGTATCCTTGCTAAAGGTATTGAAAAAGGTATTGCTAATTCAATTTTAATTAAGCTAAATCAAATTGGTACTTTGAC
AGAAACTTTCGAAGCAATGGCAATGGCTGGTCAAGCAGGATACACTTGTGTGGTATCGCATCGTTCTGGTGAAACTTCTG
ATACAATTATTGCTGATTTAGCAGTAGCTACATGTTCTGGACAAATTAAGACAGGGTCATTATCTAGATCTGATCGTATA
GCTAAGTATAACCAGCTGCTTAGAATCGAAGAAGAATTAGGTGAAAATGCAATTTACCCAGGGATAAAAGCATTTGTATT
TAATTCAGATGAAGAAGTAGAAGAAGTTGTTCAAGAAATTATTGTAGAAGATAGTGAAGCTGAGAAAGTTGTAGTTCAAG
TAGAAGAATAA

Upstream 100 bases:

>100_bases
TAAGTATATTAAATTGCAAAGCATACATAGCTAGCTTATAATTGATAAAGTTTTTGAAAAAATTGATAAAAGACTTAATT
TATCTAAGGAGTTTGTAATT

Downstream 100 bases:

>100_bases
TTATTTCATGGATATCAAATCTAACTCTTTTTTTTATATTTTCATTTCTGTAGTTTTATTACTAATAGCAATATTGCAAT
ATGATCTGTGGTTTAGTAAT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSSQIKQVFARQILDSRGNPTVEVDVVLESGAFGRAAVPSGASTGIREALELRDGNKALFLGKSVYKAVENVNTKIAQAV
KGLDALDQRLIDKTMIELDGSENKKNLGANAILGVSLATARAAASHLRKPFYRYLMDVKEYLMPVPMMNVINGGSHADNN
VDMQEFMIVPAGFDTFSEALRCGTEVFHILKKVLIADGYSVAGVGDEGGYAPDLPSNEAAIEAILKAVKEAGYEPGKHVF
IALDPASSEFYKDGKYELKSENKSLTSEEMIDYYAAWVEKYPIVSIEDGLAEEDWAGWKLLTEKLGNKVQLVGDDLFVTN
PSILAKGIEKGIANSILIKLNQIGTLTETFEAMAMAGQAGYTCVVSHRSGETSDTIIADLAVATCSGQIKTGSLSRSDRI
AKYNQLLRIEEELGENAIYPGIKAFVFNSDEEVEEVVQEIIVEDSEAEKVVVQVEE

Sequences:

>Translated_456_residues
MSSQIKQVFARQILDSRGNPTVEVDVVLESGAFGRAAVPSGASTGIREALELRDGNKALFLGKSVYKAVENVNTKIAQAV
KGLDALDQRLIDKTMIELDGSENKKNLGANAILGVSLATARAAASHLRKPFYRYLMDVKEYLMPVPMMNVINGGSHADNN
VDMQEFMIVPAGFDTFSEALRCGTEVFHILKKVLIADGYSVAGVGDEGGYAPDLPSNEAAIEAILKAVKEAGYEPGKHVF
IALDPASSEFYKDGKYELKSENKSLTSEEMIDYYAAWVEKYPIVSIEDGLAEEDWAGWKLLTEKLGNKVQLVGDDLFVTN
PSILAKGIEKGIANSILIKLNQIGTLTETFEAMAMAGQAGYTCVVSHRSGETSDTIIADLAVATCSGQIKTGSLSRSDRI
AKYNQLLRIEEELGENAIYPGIKAFVFNSDEEVEEVVQEIIVEDSEAEKVVVQVEE
>Mature_455_residues
SSQIKQVFARQILDSRGNPTVEVDVVLESGAFGRAAVPSGASTGIREALELRDGNKALFLGKSVYKAVENVNTKIAQAVK
GLDALDQRLIDKTMIELDGSENKKNLGANAILGVSLATARAAASHLRKPFYRYLMDVKEYLMPVPMMNVINGGSHADNNV
DMQEFMIVPAGFDTFSEALRCGTEVFHILKKVLIADGYSVAGVGDEGGYAPDLPSNEAAIEAILKAVKEAGYEPGKHVFI
ALDPASSEFYKDGKYELKSENKSLTSEEMIDYYAAWVEKYPIVSIEDGLAEEDWAGWKLLTEKLGNKVQLVGDDLFVTNP
SILAKGIEKGIANSILIKLNQIGTLTETFEAMAMAGQAGYTCVVSHRSGETSDTIIADLAVATCSGQIKTGSLSRSDRIA
KYNQLLRIEEELGENAIYPGIKAFVFNSDEEVEEVVQEIIVEDSEAEKVVVQVEE

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=53.0232558139535, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=53.0232558139535, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=50.8158508158508, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI4503571, Length=428, Percent_Identity=51.1682242990654, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=47.8971962616822, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI169201331, Length=355, Percent_Identity=27.6056338028169, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI169201757, Length=355, Percent_Identity=27.6056338028169, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI239744207, Length=355, Percent_Identity=27.6056338028169, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=63.849765258216, Blast_Score=513, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=52.9137529137529, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=52.9137529137529, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=49.2063492063492, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=51.9721577726218, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=50.9259259259259, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=50.6944444444444, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=50.6944444444444, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=51.7401392111369, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580918, Length=433, Percent_Identity=51.270207852194, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=433, Percent_Identity=51.270207852194, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=433, Percent_Identity=51.270207852194, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=433, Percent_Identity=51.270207852194, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=433, Percent_Identity=51.270207852194, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17137654, Length=433, Percent_Identity=51.270207852194, Blast_Score=404, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 49510; Mature: 49378

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00164 ENOLASE ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQIKQVFARQILDSRGNPTVEVDVVLESGAFGRAAVPSGASTGIREALELRDGNKALF
CCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEE
LGKSVYKAVENVNTKIAQAVKGLDALDQRLIDKTMIELDGSENKKNLGANAILGVSLATA
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHH
RAAASHLRKPFYRYLMDVKEYLMPVPMMNVINGGSHADNNVDMQEFMIVPAGFDTFSEAL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHEEEECCCHHHHHHHH
RCGTEVFHILKKVLIADGYSVAGVGDEGGYAPDLPSNEAAIEAILKAVKEAGYEPGKHVF
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
IALDPASSEFYKDGKYELKSENKSLTSEEMIDYYAAWVEKYPIVSIEDGLAEEDWAGWKL
EEECCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHH
LTEKLGNKVQLVGDDLFVTNPSILAKGIEKGIANSILIKLNQIGTLTETFEAMAMAGQAG
HHHHHCCEEEEEECCEEEECHHHHHHHHHHHHHHHHEEEEHHCCCHHHHHHHHHHCCCCC
YTCVVSHRSGETSDTIIADLAVATCSGQIKTGSLSRSDRIAKYNQLLRIEEELGENAIYP
CEEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GIKAFVFNSDEEVEEVVQEIIVEDSEAEKVVVQVEE
CCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECC
>Mature Secondary Structure 
SSQIKQVFARQILDSRGNPTVEVDVVLESGAFGRAAVPSGASTGIREALELRDGNKALF
CHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEE
LGKSVYKAVENVNTKIAQAVKGLDALDQRLIDKTMIELDGSENKKNLGANAILGVSLATA
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHH
RAAASHLRKPFYRYLMDVKEYLMPVPMMNVINGGSHADNNVDMQEFMIVPAGFDTFSEAL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHEEEECCCHHHHHHHH
RCGTEVFHILKKVLIADGYSVAGVGDEGGYAPDLPSNEAAIEAILKAVKEAGYEPGKHVF
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
IALDPASSEFYKDGKYELKSENKSLTSEEMIDYYAAWVEKYPIVSIEDGLAEEDWAGWKL
EEECCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHH
LTEKLGNKVQLVGDDLFVTNPSILAKGIEKGIANSILIKLNQIGTLTETFEAMAMAGQAG
HHHHHCCEEEEEECCEEEECHHHHHHHHHHHHHHHHEEEEHHCCCHHHHHHHHHHCCCCC
YTCVVSHRSGETSDTIIADLAVATCSGQIKTGSLSRSDRIAKYNQLLRIEEELGENAIYP
CEEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GIKAFVFNSDEEVEEVVQEIIVEDSEAEKVVVQVEE
CCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA