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The map label for this gene is ispD [H]

Identifier: 134302397

GI number: 134302397

Start: 1437862

End: 1438551

Strand: Reverse

Name: ispD [H]

Synonym: FTW_1530

Alternate gene names: 134302397

Gene position: 1438551-1437862 (Counterclockwise)

Preceding gene: 134302398

Following gene: 134302393

Centisome position: 75.77

GC content: 31.88

Gene sequence:

>690_bases
GTGTCAAATAAATACGTAATTATTCCAGCTGCTGGAATAGGTACTAGGATGCAGTTAGATATTCCTAAACAATACTATAA
ACTTAATAATGGTAAGACTATCCTTGATAATACCTTAGTAAAGTTTATTGATAATCCTTTGTTTGATAAGATCTTTGTTG
CAATTGCTGCTAGTGATAATTTTTGGAATAATTCGTTATATTATAATCATGACAAAATAGTAGTTTGTAATGGTGGAGAG
ACTAGATTTAATAGTGTTTATAATGCGCTAAAGGTCATTGATGAACGCAAAAATGATGATTGGGTTTTTGTCCATGATGC
TGCCAGACCTTGTGTTAGTATTGATAGTATTATAGATTTGTATGAGCAGACTAAATCATCACACTCACAAGCAGGTATAC
TTGCTGTGAGAGCGTATGAGACAGTTAAGCAAGTTACAAAGAATATAGTTGTCAAAACACTGGCTCGTGATAATATTTGG
CTTGCACAAACACCTCAATTATCTAGGCTTGGACAGTTAGAGAAAGCTTTTGATTTTTGCTATTCAAATAATCTTGTTGC
TAAAGTAACTGATGAAGCATCAGCTTTAGAAATGTTTGGTATAAATCCGATTGTTGTTGAATGTTCAAAGAAAAATATTA
AGATTACAACTAAAGATGATTTAGAATATGCTAATTGGCAGTTAGGTTAA

Upstream 100 bases:

>100_bases
TTATATTCTGAAGTGGTTTCACTACGTCAAAATAGTGAGGTGCTTGAAAGCTTAGCTCGTGAGAATATGGGTCTAATCAA
GCAAGGAGAGGTTTTTTATA

Downstream 100 bases:

>100_bases
GTCGTTATTTTAGAAGTAAACCAATTACATAGCCAAATATAATTATTAATCCCATTATAAATAAAATAGAGTCTAATATT
ACGCGTCTATAATCTTTACA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSNKYVIIPAAGIGTRMQLDIPKQYYKLNNGKTILDNTLVKFIDNPLFDKIFVAIAASDNFWNNSLYYNHDKIVVCNGGE
TRFNSVYNALKVIDERKNDDWVFVHDAARPCVSIDSIIDLYEQTKSSHSQAGILAVRAYETVKQVTKNIVVKTLARDNIW
LAQTPQLSRLGQLEKAFDFCYSNNLVAKVTDEASALEMFGINPIVVECSKKNIKITTKDDLEYANWQLG

Sequences:

>Translated_229_residues
MSNKYVIIPAAGIGTRMQLDIPKQYYKLNNGKTILDNTLVKFIDNPLFDKIFVAIAASDNFWNNSLYYNHDKIVVCNGGE
TRFNSVYNALKVIDERKNDDWVFVHDAARPCVSIDSIIDLYEQTKSSHSQAGILAVRAYETVKQVTKNIVVKTLARDNIW
LAQTPQLSRLGQLEKAFDFCYSNNLVAKVTDEASALEMFGINPIVVECSKKNIKITTKDDLEYANWQLG
>Mature_228_residues
SNKYVIIPAAGIGTRMQLDIPKQYYKLNNGKTILDNTLVKFIDNPLFDKIFVAIAASDNFWNNSLYYNHDKIVVCNGGET
RFNSVYNALKVIDERKNDDWVFVHDAARPCVSIDSIIDLYEQTKSSHSQAGILAVRAYETVKQVTKNIVVKTLARDNIWL
AQTPQLSRLGQLEKAFDFCYSNNLVAKVTDEASALEMFGINPIVVECSKKNIKITTKDDLEYANWQLG

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=225, Percent_Identity=36, Blast_Score=143, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 25924; Mature: 25793

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKYVIIPAAGIGTRMQLDIPKQYYKLNNGKTILDNTLVKFIDNPLFDKIFVAIAASDN
CCCCEEEEECCCCCCEEEECCCHHHEEECCCCEEHHHHHHHHHCCCHHHEEEEEEEECCC
FWNNSLYYNHDKIVVCNGGETRFNSVYNALKVIDERKNDDWVFVHDAARPCVSIDSIIDL
CCCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHH
YEQTKSSHSQAGILAVRAYETVKQVTKNIVVKTLARDNIWLAQTPQLSRLGQLEKAFDFC
HHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHH
YSNNLVAKVTDEASALEMFGINPIVVECSKKNIKITTKDDLEYANWQLG
HCCCEEEEECCCCHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCEECC
>Mature Secondary Structure 
SNKYVIIPAAGIGTRMQLDIPKQYYKLNNGKTILDNTLVKFIDNPLFDKIFVAIAASDN
CCCEEEEECCCCCCEEEECCCHHHEEECCCCEEHHHHHHHHHCCCHHHEEEEEEEECCC
FWNNSLYYNHDKIVVCNGGETRFNSVYNALKVIDERKNDDWVFVHDAARPCVSIDSIIDL
CCCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHH
YEQTKSSHSQAGILAVRAYETVKQVTKNIVVKTLARDNIWLAQTPQLSRLGQLEKAFDFC
HHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHH
YSNNLVAKVTDEASALEMFGINPIVVECSKKNIKITTKDDLEYANWQLG
HCCCEEEEECCCCHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA