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The map label for this gene is proP [H]

Identifier: 134302391

GI number: 134302391

Start: 1427813

End: 1429033

Strand: Reverse

Name: proP [H]

Synonym: FTW_1520

Alternate gene names: 134302391

Gene position: 1429033-1427813 (Counterclockwise)

Preceding gene: 134302392

Following gene: 134302383

Centisome position: 75.27

GC content: 31.2

Gene sequence:

>1221_bases
ATGAAAAATTATAATAGGTTGTTATTTTCAACTTTAGGGGCAGCTTTTGAATATTATGATCTAGCAATATATTCTGTATT
TGCTGTGACTATTGGGAGTAAGTTTTTTGATAAAAGTAGTTCTATTAGTAATACTTTATTAGTATTTTTGGTTTATATTG
TTGGTTATATTGTACGCCCACTTGGTGCTTGGGGATTTAGTTATATTGCTGATAAGAAAGGTAGAGCGTTTATATTACGA
TTAAATATGATTTTACTATTTTTCTCCACTTTAATATTAGCTTTGTTACCTACTATCGAAAGTATCGGAGTCTTAGCAAC
TGTTTTATTTGTAGGTTTACGCTGCGTACAGTCATTAGCAGTTGGAGCTGAAATACCTGTTTCAGTAGTATATATAGTTG
AGAACTATCCAAAAAGACAAGGCTTAGTGACTAGCATGGTATTTTGCTGCTTAAGTATAGGTATTATGATGACATCATTA
GTGTTGTTTATTTTAAATCATTTTACTAGCCAAACGTTTATACAAGATTATGGTTGGCGTATAGGATTTTTTATAGGCGC
TTGTTTTACATTTATACTATTTTTCTTAAGAAAAAACATTATTGATATTCCACAAGTTACCAATAGCTTAGATAAGCCGC
AATCTGAGAATTATATTTTCATTATAAAGGTGATTATTGGTATAGCCTTAGTCGCTTGTATTGCTATGCTAACTACACAG
CTGTATATGTTTTTACCAGCATATCACCAGATGTATATACAAAGTAGCTATGATGTTTCTGACTTACTGTTGACAGGTTC
TATAATTATGGCAATTAGTTGTTTGATTGGTGGATTTATTAGTGACTATGTATCTAAATCTAAGATGATGGCTTTGTTAA
TATTGATAAGCATAGCCGTAGCGCCAATTTTTTATAAGAATATGATCGCAGGAACAGATGTTTTTGCATGCTTTATTATT
CTATCTATAGTAATGGGCTTTTTTGCTCCTACTTATAATGTAATTATTACAAATTTCTTTAGGTTGGAATATAGGTGTCG
TGGTTATGGTATAGCTTATAATTTAGGTTATTTAGTGTTTTCTGCAGGTGTTCCAGCATTGACAATATCTCTTATTACAG
CAACTAGTAGTCTATTAGTACCAGTATATTTAATCGTATTTGCTGCAGCAGTATCTTTTATTGGAATAGCTGCGGCAAGT
TGGTTTATGAAAAAGGCTTAA

Upstream 100 bases:

>100_bases
CATTAGAAATTATCGAGCTTTCACCTGCTTATGCTAATTAGTATTTGATTATCTTTTTTATTTTATAATATATATAAAAA
TCTTTAAATTTTGATAATTG

Downstream 100 bases:

>100_bases
AAATCATCGCTGAAGGATCAGATTTGAACTGATTATAAAGTTCGATTATATAGCGGTCAGTCATTGAAGATATATTGTCG
CTAATTATTCTAGCTTTAGC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MKNYNRLLFSTLGAAFEYYDLAIYSVFAVTIGSKFFDKSSSISNTLLVFLVYIVGYIVRPLGAWGFSYIADKKGRAFILR
LNMILLFFSTLILALLPTIESIGVLATVLFVGLRCVQSLAVGAEIPVSVVYIVENYPKRQGLVTSMVFCCLSIGIMMTSL
VLFILNHFTSQTFIQDYGWRIGFFIGACFTFILFFLRKNIIDIPQVTNSLDKPQSENYIFIIKVIIGIALVACIAMLTTQ
LYMFLPAYHQMYIQSSYDVSDLLLTGSIIMAISCLIGGFISDYVSKSKMMALLILISIAVAPIFYKNMIAGTDVFACFII
LSIVMGFFAPTYNVIITNFFRLEYRCRGYGIAYNLGYLVFSAGVPALTISLITATSSLLVPVYLIVFAAAVSFIGIAAAS
WFMKKA

Sequences:

>Translated_406_residues
MKNYNRLLFSTLGAAFEYYDLAIYSVFAVTIGSKFFDKSSSISNTLLVFLVYIVGYIVRPLGAWGFSYIADKKGRAFILR
LNMILLFFSTLILALLPTIESIGVLATVLFVGLRCVQSLAVGAEIPVSVVYIVENYPKRQGLVTSMVFCCLSIGIMMTSL
VLFILNHFTSQTFIQDYGWRIGFFIGACFTFILFFLRKNIIDIPQVTNSLDKPQSENYIFIIKVIIGIALVACIAMLTTQ
LYMFLPAYHQMYIQSSYDVSDLLLTGSIIMAISCLIGGFISDYVSKSKMMALLILISIAVAPIFYKNMIAGTDVFACFII
LSIVMGFFAPTYNVIITNFFRLEYRCRGYGIAYNLGYLVFSAGVPALTISLITATSSLLVPVYLIVFAAAVSFIGIAAAS
WFMKKA
>Mature_406_residues
MKNYNRLLFSTLGAAFEYYDLAIYSVFAVTIGSKFFDKSSSISNTLLVFLVYIVGYIVRPLGAWGFSYIADKKGRAFILR
LNMILLFFSTLILALLPTIESIGVLATVLFVGLRCVQSLAVGAEIPVSVVYIVENYPKRQGLVTSMVFCCLSIGIMMTSL
VLFILNHFTSQTFIQDYGWRIGFFIGACFTFILFFLRKNIIDIPQVTNSLDKPQSENYIFIIKVIIGIALVACIAMLTTQ
LYMFLPAYHQMYIQSSYDVSDLLLTGSIIMAISCLIGGFISDYVSKSKMMALLILISIAVAPIFYKNMIAGTDVFACFII
LSIVMGFFAPTYNVIITNFFRLEYRCRGYGIAYNLGYLVFSAGVPALTISLITATSSLLVPVYLIVFAAAVSFIGIAAAS
WFMKKA

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=411, Percent_Identity=23.6009732360097, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788942, Length=385, Percent_Identity=24.9350649350649, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788292, Length=382, Percent_Identity=23.8219895287958, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789941, Length=207, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45075; Mature: 45075

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA