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The map label for this gene is rep [H]

Identifier: 134302295

GI number: 134302295

Start: 1303624

End: 1305639

Strand: Reverse

Name: rep [H]

Synonym: FTW_1393

Alternate gene names: 134302295

Gene position: 1305639-1303624 (Counterclockwise)

Preceding gene: 134302296

Following gene: 134302294

Centisome position: 68.77

GC content: 31.65

Gene sequence:

>2016_bases
ATGCTAGATAATCTTAATCAACAGCAGCAACAAGCTGTCAAGTATATAGCAACACCATTACTTGTATTAGCAGGTGCAGG
TAGTGGTAAAACTAGTGTAATTATAGAGAAAATTTCTTATCTTATTGAACAGCTTTTATATCCTGCAAAAAGCATTTTAG
CGGTGACTTTTACTAATAAAGCAGCTAAAGAAATGCAAGAGCGTGTCAAATCGCGCCTTGATAAAGAAAAGTCAAAAGGC
TTGATGATATCTACTTTTCACTCGCTTGGTTTATCAATTCTGAAAAAGCATTTTAACGAGCTAGGATATAAGAAGAATTT
TACGCTATTTGATAGCCATGATTCTTTAGCTTTAATATATGATATTGCCTATGATGAATATCAGCTACCTAAGCAGAATG
CTAGTTTTATCCAAGCAAAAATATCATTTTGGAAGTCTGCTTTACTTACTCCTGATCAAGTTAAGCCTAAAGATGAATTA
GAAGAGCAGGCAGCTTTCATTTACCAAGAATATCAAAAATACCTAAAATCATATAACTCTTTTGATTTTGATGATCTAAT
ATTTCAACCAATACAGTTGTTTAGGAATCATTCTAATATCCAGAAACTTTGGGCAGATAAGTTTAGGTATATATTGATAG
ATGAATATCAAGATACTAATGAATCGCAATATCAGCTTTTAAAATATTTAACTCTAGGAAAAAATAAATTTACTGTAGTA
GGTGATGATGATCAATCTATATACGCATGGCGTGGTTCAAGACCAGAAAATTTACGCCATTTACAAGAAGATTTTGCTGA
TCTAAAGGTTATCAAATTAGAGCAGAATTATCGTTCTACAGGTAGAATTTTAAATGTTGCTAATAAGCTTATAGAAAATA
ATAGCCATATTTTTGACAAAAAACTTTGGTCTAATAAAGACTATGGTGAGCAGATTAAGGTAATTAGTTTGATAAATGAT
GAGGATGAGGCACAATTTATAGCTAGTGATATATTTTTTGATAGAGTTAAGACGAAATCAAAAAATTCTGATTATGCGGT
ACTAATTAGGAGTAATTATCAAGCATATCTACTTGAAAGATATATGCAAATGCACAAAATTCCATATACTATCAGTGGTG
GCAGCTCATTTTTTTCTAAAGCCGAAATCAAAGATATTATTTCGTATTTGAGGTTAATTGTTAATCCTGATGATGATAGA
GCTTTTTTACGCGTGATAAATACACCTAAACGAGAGGTTGGTAGTGCGACTGTTCACAAGTTAGGAGAGTATGCAAGCCA
GCATCATTGTAGTTTTTTTCATACATTGTATAGTTTAGAAAATTTTGAATTACGTGATTTTACTAAGAGAAATCTAATAT
CTTTTAAGGATTTAATACTCAACACTCAACAGCAGATAAACACAAGTATCTCAGTACAAGAGTTAAAAAATATAATTAAT
AGTTTTATTGATAATATTTCATATAGACAATGGTTAATAGACTCTAGTTCTTCTGAAAAGCAAGCTGAGTTTAGGTATGC
AAATATTATTGAAGTTACGAAATGGATTGTAAATCAACTTGAGGATGAATCATATAATGGGCTTGAGTCATTAGCAACCG
TATTGAATAAAATGCTATTGATAGATATTTTAGATAGAGATAATGAAGATAAAAATGACAATCAAGTTCAGATTATAACT
ATGCATGCCTCGAAAGGTCTAGAATTTAAAAAAGTCTATATCATGGGAATGGAAGAGGGGATATTACCACATCAACAAAG
TATTGAGGATGATTCAATAGAAGATGAGCGTCGCCTTGCCTATGTCGCGATTACTCGAGCTAGAGAGAATCTCACCATCA
CGATGACAAAACATCGTAAAAAATTTGGTGAAAAACAAGTTTCAGTTCCAAGTAGATTTATTGATGAGTTACCAGAGACA
GATCTATACTGGGTTGGCACAGAAAAAGAATGTGCAGAAACCCGTAAGCAAAATTCAAAACAAAATATTTCAGCATTAAA
GGATATGTTTGGCTAG

Upstream 100 bases:

>100_bases
ATAAAGTATTTGTTGATAACCAGTGCTATATGCTAGTTGTGTTTCATAAAGAAGCTTCTGAGCAAGAGTATCAATTAGAT
GATGGCAAAATTATAACTAA

Downstream 100 bases:

>100_bases
TTTAAGCAAATATATGGTATGATTTACCTGCATAAAAATATCAAAGGTAATTATTTTGATTTCAGTTGGAAAATATCACA
AATTAGAAGTTCTCGATAAA

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MLDNLNQQQQQAVKYIATPLLVLAGAGSGKTSVIIEKISYLIEQLLYPAKSILAVTFTNKAAKEMQERVKSRLDKEKSKG
LMISTFHSLGLSILKKHFNELGYKKNFTLFDSHDSLALIYDIAYDEYQLPKQNASFIQAKISFWKSALLTPDQVKPKDEL
EEQAAFIYQEYQKYLKSYNSFDFDDLIFQPIQLFRNHSNIQKLWADKFRYILIDEYQDTNESQYQLLKYLTLGKNKFTVV
GDDDQSIYAWRGSRPENLRHLQEDFADLKVIKLEQNYRSTGRILNVANKLIENNSHIFDKKLWSNKDYGEQIKVISLIND
EDEAQFIASDIFFDRVKTKSKNSDYAVLIRSNYQAYLLERYMQMHKIPYTISGGSSFFSKAEIKDIISYLRLIVNPDDDR
AFLRVINTPKREVGSATVHKLGEYASQHHCSFFHTLYSLENFELRDFTKRNLISFKDLILNTQQQINTSISVQELKNIIN
SFIDNISYRQWLIDSSSSEKQAEFRYANIIEVTKWIVNQLEDESYNGLESLATVLNKMLLIDILDRDNEDKNDNQVQIIT
MHASKGLEFKKVYIMGMEEGILPHQQSIEDDSIEDERRLAYVAITRARENLTITMTKHRKKFGEKQVSVPSRFIDELPET
DLYWVGTEKECAETRKQNSKQNISALKDMFG

Sequences:

>Translated_671_residues
MLDNLNQQQQQAVKYIATPLLVLAGAGSGKTSVIIEKISYLIEQLLYPAKSILAVTFTNKAAKEMQERVKSRLDKEKSKG
LMISTFHSLGLSILKKHFNELGYKKNFTLFDSHDSLALIYDIAYDEYQLPKQNASFIQAKISFWKSALLTPDQVKPKDEL
EEQAAFIYQEYQKYLKSYNSFDFDDLIFQPIQLFRNHSNIQKLWADKFRYILIDEYQDTNESQYQLLKYLTLGKNKFTVV
GDDDQSIYAWRGSRPENLRHLQEDFADLKVIKLEQNYRSTGRILNVANKLIENNSHIFDKKLWSNKDYGEQIKVISLIND
EDEAQFIASDIFFDRVKTKSKNSDYAVLIRSNYQAYLLERYMQMHKIPYTISGGSSFFSKAEIKDIISYLRLIVNPDDDR
AFLRVINTPKREVGSATVHKLGEYASQHHCSFFHTLYSLENFELRDFTKRNLISFKDLILNTQQQINTSISVQELKNIIN
SFIDNISYRQWLIDSSSSEKQAEFRYANIIEVTKWIVNQLEDESYNGLESLATVLNKMLLIDILDRDNEDKNDNQVQIIT
MHASKGLEFKKVYIMGMEEGILPHQQSIEDDSIEDERRLAYVAITRARENLTITMTKHRKKFGEKQVSVPSRFIDELPET
DLYWVGTEKECAETRKQNSKQNISALKDMFG
>Mature_671_residues
MLDNLNQQQQQAVKYIATPLLVLAGAGSGKTSVIIEKISYLIEQLLYPAKSILAVTFTNKAAKEMQERVKSRLDKEKSKG
LMISTFHSLGLSILKKHFNELGYKKNFTLFDSHDSLALIYDIAYDEYQLPKQNASFIQAKISFWKSALLTPDQVKPKDEL
EEQAAFIYQEYQKYLKSYNSFDFDDLIFQPIQLFRNHSNIQKLWADKFRYILIDEYQDTNESQYQLLKYLTLGKNKFTVV
GDDDQSIYAWRGSRPENLRHLQEDFADLKVIKLEQNYRSTGRILNVANKLIENNSHIFDKKLWSNKDYGEQIKVISLIND
EDEAQFIASDIFFDRVKTKSKNSDYAVLIRSNYQAYLLERYMQMHKIPYTISGGSSFFSKAEIKDIISYLRLIVNPDDDR
AFLRVINTPKREVGSATVHKLGEYASQHHCSFFHTLYSLENFELRDFTKRNLISFKDLILNTQQQINTSISVQELKNIIN
SFIDNISYRQWLIDSSSSEKQAEFRYANIIEVTKWIVNQLEDESYNGLESLATVLNKMLLIDILDRDNEDKNDNQVQIIT
MHASKGLEFKKVYIMGMEEGILPHQQSIEDDSIEDERRLAYVAITRARENLTITMTKHRKKFGEKQVSVPSRFIDELPET
DLYWVGTEKECAETRKQNSKQNISALKDMFG

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=673, Percent_Identity=45.7652303120357, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI2367296, Length=653, Percent_Identity=36.906584992343, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1787196, Length=426, Percent_Identity=26.056338028169, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=461, Percent_Identity=29.2841648590022, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6324477, Length=692, Percent_Identity=23.5549132947977, Blast_Score=94, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78173; Mature: 78173

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDNLNQQQQQAVKYIATPLLVLAGAGSGKTSVIIEKISYLIEQLLYPAKSILAVTFTNK
CCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCHHHEEEEEECCH
AAKEMQERVKSRLDKEKSKGLMISTFHSLGLSILKKHFNELGYKKNFTLFDSHDSLALIY
HHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEE
DIAYDEYQLPKQNASFIQAKISFWKSALLTPDQVKPKDELEEQAAFIYQEYQKYLKSYNS
EECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
FDFDDLIFQPIQLFRNHSNIQKLWADKFRYILIDEYQDTNESQYQLLKYLTLGKNKFTVV
CCHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEE
GDDDQSIYAWRGSRPENLRHLQEDFADLKVIKLEQNYRSTGRILNVANKLIENNSHIFDK
CCCCCEEEEECCCCCHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHH
KLWSNKDYGEQIKVISLINDEDEAQFIASDIFFDRVKTKSKNSDYAVLIRSNYQAYLLER
HHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
YMQMHKIPYTISGGSSFFSKAEIKDIISYLRLIVNPDDDRAFLRVINTPKREVGSATVHK
HHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHH
LGEYASQHHCSFFHTLYSLENFELRDFTKRNLISFKDLILNTQQQINTSISVQELKNIIN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHH
SFIDNISYRQWLIDSSSSEKQAEFRYANIIEVTKWIVNQLEDESYNGLESLATVLNKMLL
HHHHCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
IDILDRDNEDKNDNQVQIITMHASKGLEFKKVYIMGMEEGILPHQQSIEDDSIEDERRLA
HHHHCCCCCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHEE
YVAITRARENLTITMTKHRKKFGEKQVSVPSRFIDELPETDLYWVGTEKECAETRKQNSK
EEEEEECCCCEEEEEEHHHHHHCCHHCCCHHHHHHHCCCCCEEEECCCHHHHHHHHCCCH
QNISALKDMFG
HHHHHHHHHCC
>Mature Secondary Structure
MLDNLNQQQQQAVKYIATPLLVLAGAGSGKTSVIIEKISYLIEQLLYPAKSILAVTFTNK
CCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCHHHEEEEEECCH
AAKEMQERVKSRLDKEKSKGLMISTFHSLGLSILKKHFNELGYKKNFTLFDSHDSLALIY
HHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEE
DIAYDEYQLPKQNASFIQAKISFWKSALLTPDQVKPKDELEEQAAFIYQEYQKYLKSYNS
EECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
FDFDDLIFQPIQLFRNHSNIQKLWADKFRYILIDEYQDTNESQYQLLKYLTLGKNKFTVV
CCHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEE
GDDDQSIYAWRGSRPENLRHLQEDFADLKVIKLEQNYRSTGRILNVANKLIENNSHIFDK
CCCCCEEEEECCCCCHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHH
KLWSNKDYGEQIKVISLINDEDEAQFIASDIFFDRVKTKSKNSDYAVLIRSNYQAYLLER
HHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
YMQMHKIPYTISGGSSFFSKAEIKDIISYLRLIVNPDDDRAFLRVINTPKREVGSATVHK
HHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHH
LGEYASQHHCSFFHTLYSLENFELRDFTKRNLISFKDLILNTQQQINTSISVQELKNIIN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHH
SFIDNISYRQWLIDSSSSEKQAEFRYANIIEVTKWIVNQLEDESYNGLESLATVLNKMLL
HHHHCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
IDILDRDNEDKNDNQVQIITMHASKGLEFKKVYIMGMEEGILPHQQSIEDDSIEDERRLA
HHHHCCCCCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHEE
YVAITRARENLTITMTKHRKKFGEKQVSVPSRFIDELPETDLYWVGTEKECAETRKQNSK
EEEEEECCCCEEEEEEHHHHHHCCHHCCCHHHHHHHCCCCCEEEECCCHHHHHHHHCCCH
QNISALKDMFG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]