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The map label for this gene is folD

Identifier: 134302217

GI number: 134302217

Start: 1220311

End: 1221159

Strand: Reverse

Name: folD

Synonym: FTW_1287

Alternate gene names: 134302217

Gene position: 1221159-1220311 (Counterclockwise)

Preceding gene: 134302218

Following gene: 134302216

Centisome position: 64.32

GC content: 37.69

Gene sequence:

>849_bases
ATGATTTTAATCGATGGTAAGTCTCTCTCAAAAGACCTTAAAGAGAGATTAGCAACTCAAGTTCAAGAATATAAGCATCA
TACAGCAATCACCCCAAAACTTGTCGCAATAATTGTTGGTAATGATCCGGCAAGTAAGACATATGTTGCCTCAAAAGAAA
AAGCCTGTGCCCAAGTTGGTATAGATTCTCAAGTGATTACACTACCAGAGCATACTACAGAATCAGAACTTTTGGAGCTG
ATCGATCAGCTAAATAATGACTCTAGTGTTCATGCGATTTTAGTGCAGCTACCATTACCAGCACATATCAACAAAAACAA
TGTGATATATAGTATAAAACCAGAAAAAGATGTCGATGGTTTTCATCCTACAAATGTTGGTAGACTACAACTTAGAGATA
AAAAATGCTTAGAATCTTGTACACCTAAAGGTATCATGACTATGCTAAGAGAGTATGGTATAAAAACAGAGGGGGCCTAT
GCAGTGGTTGTCGGCGCAAGTAATGTTGTTGGTAAACCAGTATCACAATTGCTGCTAAATGCTAAAGCTACGGTGACGAC
TTGTCATAGATTCACAACTGATCTTAAATCACATACTACAAAAGCAGATATCCTAATTGTTGCTGTTGGTAAGCCTAACT
TTATCACAGCTGATATGGTCAAAGAGGGTGCTGTAGTTATTGATGTTGGTATTAATCATGTTGATGGTAAGATAGTTGGT
GATGTTGATTTTGCTGCTGTAAAAGATAAGGTCGCAGCAATTACCCCAGTACCTGGAGGGGTTGGACCAATGACAATTAC
TGAACTGTTATATAATACTTTTCAGTGTGCTCAGGAGTTGAATAGGTAG

Upstream 100 bases:

>100_bases
AAACTTTATAAAAATACCCTCAAAAAAAATCCCAAAGATAGTCTAAAATTTGTTATCATATTAACTATATTTTTCAGTTA
TACAGATAAGCTATCAAATA

Downstream 100 bases:

>100_bases
TTTATAGATTTGATTTTATCATGGGTAGTCATACCCGCGTAGGCGGGTATCTCTACACTAATGGGTTAAATCGAAAGGAT
TCCCGCCTGTGCGGGAATGA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MILIDGKSLSKDLKERLATQVQEYKHHTAITPKLVAIIVGNDPASKTYVASKEKACAQVGIDSQVITLPEHTTESELLEL
IDQLNNDSSVHAILVQLPLPAHINKNNVIYSIKPEKDVDGFHPTNVGRLQLRDKKCLESCTPKGIMTMLREYGIKTEGAY
AVVVGASNVVGKPVSQLLLNAKATVTTCHRFTTDLKSHTTKADILIVAVGKPNFITADMVKEGAVVIDVGINHVDGKIVG
DVDFAAVKDKVAAITPVPGGVGPMTITELLYNTFQCAQELNR

Sequences:

>Translated_282_residues
MILIDGKSLSKDLKERLATQVQEYKHHTAITPKLVAIIVGNDPASKTYVASKEKACAQVGIDSQVITLPEHTTESELLEL
IDQLNNDSSVHAILVQLPLPAHINKNNVIYSIKPEKDVDGFHPTNVGRLQLRDKKCLESCTPKGIMTMLREYGIKTEGAY
AVVVGASNVVGKPVSQLLLNAKATVTTCHRFTTDLKSHTTKADILIVAVGKPNFITADMVKEGAVVIDVGINHVDGKIVG
DVDFAAVKDKVAAITPVPGGVGPMTITELLYNTFQCAQELNR
>Mature_282_residues
MILIDGKSLSKDLKERLATQVQEYKHHTAITPKLVAIIVGNDPASKTYVASKEKACAQVGIDSQVITLPEHTTESELLEL
IDQLNNDSSVHAILVQLPLPAHINKNNVIYSIKPEKDVDGFHPTNVGRLQLRDKKCLESCTPKGIMTMLREYGIKTEGAY
AVVVGASNVVGKPVSQLLLNAKATVTTCHRFTTDLKSHTTKADILIVAVGKPNFITADMVKEGAVVIDVGINHVDGKIVG
DVDFAAVKDKVAAITPVPGGVGPMTITELLYNTFQCAQELNR

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=46.4406779661017, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI222136639, Length=289, Percent_Identity=43.9446366782007, Blast_Score=234, Evalue=7e-62,
Organism=Homo sapiens, GI94721354, Length=297, Percent_Identity=46.1279461279461, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI36796743, Length=212, Percent_Identity=27.8301886792453, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=53.5971223021583, Blast_Score=292, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17568735, Length=288, Percent_Identity=38.5416666666667, Blast_Score=184, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=43.3566433566434, Blast_Score=233, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6321643, Length=294, Percent_Identity=41.156462585034, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6322933, Length=306, Percent_Identity=26.797385620915, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=46.2585034013605, Blast_Score=240, Evalue=8e-64,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=46.2585034013605, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=40.625, Blast_Score=211, Evalue=4e-55,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=40.625, Blast_Score=211, Evalue=4e-55,
Organism=Drosophila melanogaster, GI62472483, Length=288, Percent_Identity=40.625, Blast_Score=211, Evalue=4e-55,
Organism=Drosophila melanogaster, GI45551871, Length=288, Percent_Identity=40.625, Blast_Score=211, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_FRAT1 (Q14HV5)

Other databases:

- EMBL:   AM286280
- RefSeq:   YP_667021.1
- ProteinModelPortal:   Q14HV5
- SMR:   Q14HV5
- STRING:   Q14HV5
- GeneID:   4199416
- GenomeReviews:   AM286280_GR
- KEGG:   ftf:FTF0892
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- PhylomeDB:   Q14HV5
- ProtClustDB:   PRK14180
- BioCyc:   FTUL393115:FTF0892-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30455; Mature: 30455

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILIDGKSLSKDLKERLATQVQEYKHHTAITPKLVAIIVGNDPASKTYVASKEKACAQVG
CEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEECCHHHHHHHCC
IDSQVITLPEHTTESELLELIDQLNNDSSVHAILVQLPLPAHINKNNVIYSIKPEKDVDG
CCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCEECCCCEEEEECCCCCCCC
FHPTNVGRLQLRDKKCLESCTPKGIMTMLREYGIKTEGAYAVVVGASNVVGKPVSQLLLN
CCCCCCCEEEECHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHC
AKATVTTCHRFTTDLKSHTTKADILIVAVGKPNFITADMVKEGAVVIDVGINHVDGKIVG
CCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEHHHHHCCCEEEEECCCCCCCEEEE
DVDFAAVKDKVAAITPVPGGVGPMTITELLYNTFQCAQELNR
CCHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MILIDGKSLSKDLKERLATQVQEYKHHTAITPKLVAIIVGNDPASKTYVASKEKACAQVG
CEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEECCHHHHHHHCC
IDSQVITLPEHTTESELLELIDQLNNDSSVHAILVQLPLPAHINKNNVIYSIKPEKDVDG
CCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCEECCCCEEEEECCCCCCCC
FHPTNVGRLQLRDKKCLESCTPKGIMTMLREYGIKTEGAYAVVVGASNVVGKPVSQLLLN
CCCCCCCEEEECHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHC
AKATVTTCHRFTTDLKSHTTKADILIVAVGKPNFITADMVKEGAVVIDVGINHVDGKIVG
CCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEHHHHHCCCEEEEECCCCCCCEEEE
DVDFAAVKDKVAAITPVPGGVGPMTITELLYNTFQCAQELNR
CCHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA