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The map label for this gene is purK [H]

Identifier: 134302212

GI number: 134302212

Start: 1214434

End: 1215531

Strand: Reverse

Name: purK [H]

Synonym: FTW_1282

Alternate gene names: 134302212

Gene position: 1215531-1214434 (Counterclockwise)

Preceding gene: 134302213

Following gene: 134302209

Centisome position: 64.03

GC content: 36.52

Gene sequence:

>1098_bases
ATGAAAATAGGTATTATCGGAGCTGGACAACTTGCCAGGATGCTAAGCTTAGCAGGTACACCTTTAGGTTTAGAGTTTCA
TTGTCTTGGTAAAAATGGTGATTGTGCTGAAGAAGTTGTAAAAACTGTTACAGATATTGAGCTAACAAAGGTAAATGATG
TTGTAGCTTGGGCAAAGCAGTTTGATGTGATAACTTTTGAAAATGAAAATATCTCTCATGAGCTTATCAAAGCTATAAAT
CATGAGGTGAGTGTGTATCCATCAGCTAAAGCTATAGCTATTTCTCAAGATCGTTTACTTGAGAAATCCTTTATGCAAGA
TCATGGTATTGCTACTGCAAAATTTGTAAATATCGATAGTTTGGCAAAATTGCAAAGTGCTGTTGATGATCATGGTCTGC
CAGCTATACTAAAAACTCGTCGATTTGGCTATGATGGTAAGGGCCAGTTTGTGATTAGATCACAAGAGGATATTACTAAA
GCATGGGATGTGCTCAAGTATGCTCCGGATGGGCTTATTTATGAGGCTTTTGTTGATTTTGATTATGAAGTTTCACAAAT
ATGTACAGCTGATCTAAAGGGTAATATCGCCTTTTATCCATTGGCTAGAAATACTCATAAGCAGGGTATTATCGTTGAAT
CAGAAGCACCTTTTGAAAATGTTGTACTTGCTGAAAAAGCACAACAAATCGCTAAAATTCTAGTCAAGGAGTTTGCATAT
GTTGGTACTTTGGCAATTGAGTTTTTTGTCAAGGGTGATGAGCTAATAGTCAATGAAATAGCGCCACGTGTGCATAATAG
CGGTCATTGGAGTATTGATGGCGCCGTCACATCACAATTTGAAAATCATGTGCGTGCTATTGCCGGATTGATTTTAGGTG
ATACAACTAGTCGTAAGACAGTTATGCTAAATTGTATCGGTGGTATGCCGGCAACAAAAGATTTAGCAGCTCTAGATAGA
GTTAAAATTCATAGTTATAACAAGGAGCCTCGTAAGGGACGTAAAGTAGGTCATTTAAACCTTAATTTAAATGATGAAAC
TGATGAGTATCAGCTACTTCAAGTTAAAAAATTAATTGCTCTTTCTGAAGAAATTTAG

Upstream 100 bases:

>100_bases
GCAACATACAGATATAAATATTGCAAAAGCACTAGCTGAGTTTAGAGCTGAGCAAACACGATTTGTTTTGGAAAATCCTG
ATCCTAGGGAGCACTAAATA

Downstream 100 bases:

>100_bases
ATTACTCCAAAAGCATTCTTTATCCAATTAATATTACTTTCATTGATCGCAATTAAATCAAAACGACACTGATAATCTTG
GTATTGAGGATTGTGTTGTA

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKIGIIGAGQLARMLSLAGTPLGLEFHCLGKNGDCAEEVVKTVTDIELTKVNDVVAWAKQFDVITFENENISHELIKAIN
HEVSVYPSAKAIAISQDRLLEKSFMQDHGIATAKFVNIDSLAKLQSAVDDHGLPAILKTRRFGYDGKGQFVIRSQEDITK
AWDVLKYAPDGLIYEAFVDFDYEVSQICTADLKGNIAFYPLARNTHKQGIIVESEAPFENVVLAEKAQQIAKILVKEFAY
VGTLAIEFFVKGDELIVNEIAPRVHNSGHWSIDGAVTSQFENHVRAIAGLILGDTTSRKTVMLNCIGGMPATKDLAALDR
VKIHSYNKEPRKGRKVGHLNLNLNDETDEYQLLQVKKLIALSEEI

Sequences:

>Translated_365_residues
MKIGIIGAGQLARMLSLAGTPLGLEFHCLGKNGDCAEEVVKTVTDIELTKVNDVVAWAKQFDVITFENENISHELIKAIN
HEVSVYPSAKAIAISQDRLLEKSFMQDHGIATAKFVNIDSLAKLQSAVDDHGLPAILKTRRFGYDGKGQFVIRSQEDITK
AWDVLKYAPDGLIYEAFVDFDYEVSQICTADLKGNIAFYPLARNTHKQGIIVESEAPFENVVLAEKAQQIAKILVKEFAY
VGTLAIEFFVKGDELIVNEIAPRVHNSGHWSIDGAVTSQFENHVRAIAGLILGDTTSRKTVMLNCIGGMPATKDLAALDR
VKIHSYNKEPRKGRKVGHLNLNLNDETDEYQLLQVKKLIALSEEI
>Mature_365_residues
MKIGIIGAGQLARMLSLAGTPLGLEFHCLGKNGDCAEEVVKTVTDIELTKVNDVVAWAKQFDVITFENENISHELIKAIN
HEVSVYPSAKAIAISQDRLLEKSFMQDHGIATAKFVNIDSLAKLQSAVDDHGLPAILKTRRFGYDGKGQFVIRSQEDITK
AWDVLKYAPDGLIYEAFVDFDYEVSQICTADLKGNIAFYPLARNTHKQGIIVESEAPFENVVLAEKAQQIAKILVKEFAY
VGTLAIEFFVKGDELIVNEIAPRVHNSGHWSIDGAVTSQFENHVRAIAGLILGDTTSRKTVMLNCIGGMPATKDLAALDR
VKIHSYNKEPRKGRKVGHLNLNLNDETDEYQLLQVKKLIALSEEI

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=351, Percent_Identity=34.7578347578348, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1788155, Length=306, Percent_Identity=28.1045751633987, Blast_Score=105, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324702, Length=363, Percent_Identity=32.5068870523416, Blast_Score=177, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 40362; Mature: 40362

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIIGAGQLARMLSLAGTPLGLEFHCLGKNGDCAEEVVKTVTDIELTKVNDVVAWAKQ
CEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEHHHHHHHHHH
FDVITFENENISHELIKAINHEVSVYPSAKAIAISQDRLLEKSFMQDHGIATAKFVNIDS
CEEEEECCCCCHHHHHHHHCCCEEECCCCEEEEECHHHHHHHHHHHHCCCCEEEEECHHH
LAKLQSAVDDHGLPAILKTRRFGYDGKGQFVIRSQEDITKAWDVLKYAPDGLIYEAFVDF
HHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEECC
DYEVSQICTADLKGNIAFYPLARNTHKQGIIVESEAPFENVVLAEKAQQIAKILVKEFAY
CCCHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
VGTLAIEFFVKGDELIVNEIAPRVHNSGHWSIDGAVTSQFENHVRAIAGLILGDTTSRKT
HHEEEEEEEECCCEEEHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCCCE
VMLNCIGGMPATKDLAALDRVKIHSYNKEPRKGRKVGHLNLNLNDETDEYQLLQVKKLIA
EEEEEECCCCCCHHHHHHHHEEEECCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHH
LSEEI
HHCCC
>Mature Secondary Structure
MKIGIIGAGQLARMLSLAGTPLGLEFHCLGKNGDCAEEVVKTVTDIELTKVNDVVAWAKQ
CEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEHHHHHHHHHH
FDVITFENENISHELIKAINHEVSVYPSAKAIAISQDRLLEKSFMQDHGIATAKFVNIDS
CEEEEECCCCCHHHHHHHHCCCEEECCCCEEEEECHHHHHHHHHHHHCCCCEEEEECHHH
LAKLQSAVDDHGLPAILKTRRFGYDGKGQFVIRSQEDITKAWDVLKYAPDGLIYEAFVDF
HHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEECC
DYEVSQICTADLKGNIAFYPLARNTHKQGIIVESEAPFENVVLAEKAQQIAKILVKEFAY
CCCHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
VGTLAIEFFVKGDELIVNEIAPRVHNSGHWSIDGAVTSQFENHVRAIAGLILGDTTSRKT
HHEEEEEEEECCCEEEHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCCCE
VMLNCIGGMPATKDLAALDRVKIHSYNKEPRKGRKVGHLNLNLNDETDEYQLLQVKKLIA
EEEEEECCCCCCHHHHHHHHEEEECCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHH
LSEEI
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8816818; 10984043 [H]