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The map label for this gene is proP [H]

Identifier: 134302176

GI number: 134302176

Start: 1172814

End: 1174097

Strand: Reverse

Name: proP [H]

Synonym: FTW_1243

Alternate gene names: 134302176

Gene position: 1174097-1172814 (Counterclockwise)

Preceding gene: 134302177

Following gene: 134302175

Centisome position: 61.84

GC content: 33.33

Gene sequence:

>1284_bases
TTGCAAATGTATGATAGAGATAGTAGAAATGTTATATTACTTGCCGGGATTGGTGCAATCCTTGAGTTTTATGACTTTGT
TTTATATATGATTTTTTCTAAAGAAATTTCAGCAACATTTTTTGCACAAATCACTAACCCAACAATTAAAGCCTTTTTAA
CAGTTTTGATATTTTCAATAGCATATCTGGTAAGACCATTTGCAGGAACTATACTTGGGATTATTGGTGACTTGATAGGA
CGTAGGCGTCTTTTGTTATTTACGATATTACTTATGGGTGGATGTTCTTTATGTATGGGATTGATGCCTGGTTATGCTCA
ATGGGGCTTATTTGCTAGTTTTGCTTTTGTTGTTTTACGTATAATGCAAGGTATAGCTTTAGGCGGTGAACTACCAGGTG
CGTATGTGATCGTTTATGAATCTGTCAAAGGTAAAATAGGTTTTGCTACAGCGATTTTATTTACATTTGTAACTGGAGGT
TTTTTATTCTCTGATTTTGTTGGTTTTACTCTTGAGTATATTTTTGGTGATTTTGCTTGGCGAGCAGGATTTATCATTGG
CGGACTTTTGGGTTTTGTTGGCTATTATGTGCGTCGTAACCTTCATGAAACTCGAGAATTTGAGAATATTGACAAGCAAA
AAAGACATTCTTTTAGTTCTTTGATATCAACTTATGGAGCAAATTTATTTGCAGGAATATGTATGGTTATTATAGTAGCA
TTTGGCGGGGTAATGCTTACTCTTTATATCCATAAGTTTGTTGAAGATATTTTAGTCAGTTATAATTCTGGACAAATCTC
TTTAATTCTAATGCCAAGTGTTTTAACACTTACTTGTTTTACTTTTGTATTTGGTTTTTTATCAGATAAAGTTGGCATTG
CAAAAATGTTTACACTAGGAGCTGGATTTATTGTAGTTTGTGCTTTGCCAGTATTCTACTTGATGAGTAGCATTGGTAGT
GTCTCTGCTATAGTTATTACTTCAGTGATAATAATGCTTTGCTATGCTTTAGTTGCTGCTACATTTATATTTTTACTTTG
TGATCTTTTTCCTACTGATGTTAGATTATCGGGTGTAGGACTGAGCTATAATCTTGCTTTTGCTATAGTTGGTGGAGTTG
CTCCTTTGCTTAGTACAACGATTATCACAATGACAGCTTATAATTTCTTAGGTCCAGCTATTGTTGGTATAGTTTGTGGA
GTGCTTGGTCTATTAGGAATGTTTATCTATCTTAGAAAAGGTGGCTATCATAAAACTAACAAAGACATGATAGTTAAACT
ATAA

Upstream 100 bases:

>100_bases
TTTAGATAGCTACTAAAAATTATTAATACTTGTATAAAGGTTTCTTTTAAATGAAACTAAAAACTTGTATGATTTGCTAA
ACTAGTTATTAGAGCGAGTA

Downstream 100 bases:

>100_bases
CTAACAAAGGAATTCTGATAAATAGCCAATAATTTTACTATTTTAAACTAACTTTTTTGTCTTTAAAAAAACCTAGTTAA
AATATTTTTATATACTATGA

Product: major facilitator superfamily transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MQMYDRDSRNVILLAGIGAILEFYDFVLYMIFSKEISATFFAQITNPTIKAFLTVLIFSIAYLVRPFAGTILGIIGDLIG
RRRLLLFTILLMGGCSLCMGLMPGYAQWGLFASFAFVVLRIMQGIALGGELPGAYVIVYESVKGKIGFATAILFTFVTGG
FLFSDFVGFTLEYIFGDFAWRAGFIIGGLLGFVGYYVRRNLHETREFENIDKQKRHSFSSLISTYGANLFAGICMVIIVA
FGGVMLTLYIHKFVEDILVSYNSGQISLILMPSVLTLTCFTFVFGFLSDKVGIAKMFTLGAGFIVVCALPVFYLMSSIGS
VSAIVITSVIIMLCYALVAATFIFLLCDLFPTDVRLSGVGLSYNLAFAIVGGVAPLLSTTIITMTAYNFLGPAIVGIVCG
VLGLLGMFIYLRKGGYHKTNKDMIVKL

Sequences:

>Translated_427_residues
MQMYDRDSRNVILLAGIGAILEFYDFVLYMIFSKEISATFFAQITNPTIKAFLTVLIFSIAYLVRPFAGTILGIIGDLIG
RRRLLLFTILLMGGCSLCMGLMPGYAQWGLFASFAFVVLRIMQGIALGGELPGAYVIVYESVKGKIGFATAILFTFVTGG
FLFSDFVGFTLEYIFGDFAWRAGFIIGGLLGFVGYYVRRNLHETREFENIDKQKRHSFSSLISTYGANLFAGICMVIIVA
FGGVMLTLYIHKFVEDILVSYNSGQISLILMPSVLTLTCFTFVFGFLSDKVGIAKMFTLGAGFIVVCALPVFYLMSSIGS
VSAIVITSVIIMLCYALVAATFIFLLCDLFPTDVRLSGVGLSYNLAFAIVGGVAPLLSTTIITMTAYNFLGPAIVGIVCG
VLGLLGMFIYLRKGGYHKTNKDMIVKL
>Mature_427_residues
MQMYDRDSRNVILLAGIGAILEFYDFVLYMIFSKEISATFFAQITNPTIKAFLTVLIFSIAYLVRPFAGTILGIIGDLIG
RRRLLLFTILLMGGCSLCMGLMPGYAQWGLFASFAFVVLRIMQGIALGGELPGAYVIVYESVKGKIGFATAILFTFVTGG
FLFSDFVGFTLEYIFGDFAWRAGFIIGGLLGFVGYYVRRNLHETREFENIDKQKRHSFSSLISTYGANLFAGICMVIIVA
FGGVMLTLYIHKFVEDILVSYNSGQISLILMPSVLTLTCFTFVFGFLSDKVGIAKMFTLGAGFIVVCALPVFYLMSSIGS
VSAIVITSVIIMLCYALVAATFIFLLCDLFPTDVRLSGVGLSYNLAFAIVGGVAPLLSTTIITMTAYNFLGPAIVGIVCG
VLGLLGMFIYLRKGGYHKTNKDMIVKL

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=426, Percent_Identity=29.81220657277, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1788292, Length=409, Percent_Identity=26.8948655256724, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788942, Length=400, Percent_Identity=26.75, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789941, Length=398, Percent_Identity=28.391959798995, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082404, Length=389, Percent_Identity=25.1928020565553, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87082231, Length=440, Percent_Identity=24.0909090909091, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46652; Mature: 46652

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCEEEC
>Mature Secondary Structure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CCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]