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The map label for this gene is gcvA [H]

Identifier: 134302110

GI number: 134302110

Start: 1090144

End: 1091019

Strand: Direct

Name: gcvA [H]

Synonym: FTW_1152

Alternate gene names: 134302110

Gene position: 1090144-1091019 (Clockwise)

Preceding gene: 134302096

Following gene: 134302112

Centisome position: 57.42

GC content: 29.57

Gene sequence:

>876_bases
ATGATAAAAAGAAACGATCTACCTCCTCTAAATTCCTTACCGGTATTTATAGCTGTGATGAAAACCAAAAGCTACACAAA
AGCAGCTGAGCAACTATTTATGACTCATTCTGCAGTGAGCCAATCGATAAGAAAACTAGAAGCCTATCTTGATAAAAAAC
TTTTTATAACTGACAAAAGAAAGCTTATTATCACTGATTTAGCTAGAGAGTATTATGCAAAAATAGAGCCTTTAATAGCA
CAAATTCATTATTCAACTGAGGCTTTTAAAAAACAAAAATCCAAAAAGTTATCGATAAACTGCATGACTACACTATGTGC
AAACTGGTTAATTCCAAAACTTGATGAATTGATAGAATTTCTAGCAGACACAGAAATACAACTTATAACACTAGGAAGAA
AAGCAAACTTTGATTATGATGATGTAGATATTAGTATAGAGTATGGCATCGATAGCGACTTTAATAATAAAAACAAATAC
AAACTTGCTGAAGGTGAATTAATATTAGTTTGCAATAACAGACATACAGGTAAAAGCTGTTTAGAGATAATATCACAACA
AAAACTAATTTATGTAGATGATAAGGTTCGTATTGATGATTATCTTCTTTGGCAACAACATAACAATATTTTAGCTAAAG
TTAATAAAAAAATTGTTTTTAAAAATTCGCTACAAGCTATTAAGGCTTGTTTTTCAGGTATTGGATTCTTTGTTACTGAC
AAGCTTCTAATACAAGAATATATTAAAAATGGTTTCCTATATGTTCCACCACAACAAAGTTGCTCAACAGGAAAATCATA
TTACCTTTTAGCAAAAGACTCAGAAACTGAAAATTTTGATAAAATTAAAGGATTACTGACTTCTTATTTCAAGTAG

Upstream 100 bases:

>100_bases
AGTTAAATCTCTTATCTAAGATGTCTAAATAATATAACTATTGCATCGATGTGTAAAACGTATTATTCTTACAACATAAG
TAAGTTTTACTTACACATTA

Downstream 100 bases:

>100_bases
TTAGACTTATTTATAGCTTGCTAAAAACTCTTTTCTAAACTCTGAAAATCTACCTTCGTCTAGTGCCTTACGGATAGATT
TCATAAGATTTTGGTAGAAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MIKRNDLPPLNSLPVFIAVMKTKSYTKAAEQLFMTHSAVSQSIRKLEAYLDKKLFITDKRKLIITDLAREYYAKIEPLIA
QIHYSTEAFKKQKSKKLSINCMTTLCANWLIPKLDELIEFLADTEIQLITLGRKANFDYDDVDISIEYGIDSDFNNKNKY
KLAEGELILVCNNRHTGKSCLEIISQQKLIYVDDKVRIDDYLLWQQHNNILAKVNKKIVFKNSLQAIKACFSGIGFFVTD
KLLIQEYIKNGFLYVPPQQSCSTGKSYYLLAKDSETENFDKIKGLLTSYFK

Sequences:

>Translated_291_residues
MIKRNDLPPLNSLPVFIAVMKTKSYTKAAEQLFMTHSAVSQSIRKLEAYLDKKLFITDKRKLIITDLAREYYAKIEPLIA
QIHYSTEAFKKQKSKKLSINCMTTLCANWLIPKLDELIEFLADTEIQLITLGRKANFDYDDVDISIEYGIDSDFNNKNKY
KLAEGELILVCNNRHTGKSCLEIISQQKLIYVDDKVRIDDYLLWQQHNNILAKVNKKIVFKNSLQAIKACFSGIGFFVTD
KLLIQEYIKNGFLYVPPQQSCSTGKSYYLLAKDSETENFDKIKGLLTSYFK
>Mature_291_residues
MIKRNDLPPLNSLPVFIAVMKTKSYTKAAEQLFMTHSAVSQSIRKLEAYLDKKLFITDKRKLIITDLAREYYAKIEPLIA
QIHYSTEAFKKQKSKKLSINCMTTLCANWLIPKLDELIEFLADTEIQLITLGRKANFDYDDVDISIEYGIDSDFNNKNKY
KLAEGELILVCNNRHTGKSCLEIISQQKLIYVDDKVRIDDYLLWQQHNNILAKVNKKIVFKNSLQAIKACFSGIGFFVTD
KLLIQEYIKNGFLYVPPQQSCSTGKSYYLLAKDSETENFDKIKGLLTSYFK

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=286, Percent_Identity=29.020979020979, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786448, Length=255, Percent_Identity=27.843137254902, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788706, Length=256, Percent_Identity=23.046875, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33577; Mature: 33577

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRNDLPPLNSLPVFIAVMKTKSYTKAAEQLFMTHSAVSQSIRKLEAYLDKKLFITDKR
CCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
KLIITDLAREYYAKIEPLIAQIHYSTEAFKKQKSKKLSINCMTTLCANWLIPKLDELIEF
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCHHHHHHHHHH
LADTEIQLITLGRKANFDYDDVDISIEYGIDSDFNNKNKYKLAEGELILVCNNRHTGKSC
HCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCEEEEECCCCCCHHH
LEIISQQKLIYVDDKVRIDDYLLWQQHNNILAKVNKKIVFKNSLQAIKACFSGIGFFVTD
HHHHCCCCEEEECCCEEECCEEEEHHCCCCEEHHCCCEEEHHHHHHHHHHHHCCCHHHHH
KLLIQEYIKNGFLYVPPQQSCSTGKSYYLLAKDSETENFDKIKGLLTSYFK
HHHHHHHHHCCEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKRNDLPPLNSLPVFIAVMKTKSYTKAAEQLFMTHSAVSQSIRKLEAYLDKKLFITDKR
CCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
KLIITDLAREYYAKIEPLIAQIHYSTEAFKKQKSKKLSINCMTTLCANWLIPKLDELIEF
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCHHHHHHHHHH
LADTEIQLITLGRKANFDYDDVDISIEYGIDSDFNNKNKYKLAEGELILVCNNRHTGKSC
HCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCEEEEECCCCCCHHH
LEIISQQKLIYVDDKVRIDDYLLWQQHNNILAKVNKKIVFKNSLQAIKACFSGIGFFVTD
HHHHCCCCEEEECCCEEECCEEEEHHCCCCEEHHCCCEEEHHHHHHHHHHHHCCCHHHHH
KLLIQEYIKNGFLYVPPQQSCSTGKSYYLLAKDSETENFDKIKGLLTSYFK
HHHHHHHHHCCEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]