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The map label for this gene is ansA [H]

Identifier: 134302098

GI number: 134302098

Start: 1078952

End: 1079989

Strand: Reverse

Name: ansA [H]

Synonym: FTW_1138

Alternate gene names: 134302098

Gene position: 1079989-1078952 (Counterclockwise)

Preceding gene: 134302099

Following gene: 134302097

Centisome position: 56.89

GC content: 33.33

Gene sequence:

>1038_bases
ATGAAAGATCTTGAAAATCGTGCTAATAAAAAAATTTTAGTTCTATATACTGGTGGTACTATAGGTATGGTTAGTACTGA
ACAAGGCTATGATGTCAAACCTGGTTATCTTAGCGAGACAATTGCGGGTATTAGAGATTTTTATCATTATAATATGCCGC
AATTTGAGATTAAAGAGTACAGTGAACTTATAGATTCATCAAATATTAATCCTAGAAAATGGCTTTCATTAGCTTTAGAT
ATTATTAAAACTTATGAGGATTATGATGGCTTTGTAATTCTACATGGTACAGACACTCTTGCTTATACTGCTTCTGTATT
ATCATTTATGCTTGGTGAACTGCAAAAACCAGTAATTGTTACAGGATCACAAATTCCAATCTCAAAGATCAGATCAGATG
CAATATCAAATATTCTTAATAGTCTAATATTTGCTTGTAGTGATGATATCAAAGAAGTATGTGTATATTTCAATCAAAAG
CTAATGCGTGGTAATAGAACTACAAAAATATCAGCAACTGATTTTGATGCTTTTGCTTCACCAAACTATCCTGCATTGGC
AAGAGTTGGTATAGATATAGTCGTTAGGCAAGAAAAATTATGGCAGCGTGCTGATACCTTTAGTCAACCTAAGTTAGAAA
CTTTTGCTGTGCCAAAGATTGCTATTTTAAGTGTCTTTCCTGGCATGGGTAATGATATTTTAGAAGCTGTACTTGAGCCA
CCGTTACAAGGCTTAATTCTCAAGACTTATGGTGCTGGTAATATGATTAATGATCCAAGTATACATATGACACTAAAAAA
AACTAATGATAGAGGTGTTGTGATAGTAAACTGTACTCAATGTTTGTATGGAAGTGTGAAAATGGATGCTTACAAGGCTG
CTAGAGGACTTGTTGAAGCAGGCGTGGTTTCTGGTTATGATATGACAGATGAAGCAGCTTTAGGAAAGTTATTTTATTTA
TTAAGCCAGCCAAATATTACACAACAAGATGTCAAAGAAGCTTTTAATGTTTCATTACAAGGCGAAGTAACGATATAA

Upstream 100 bases:

>100_bases
GTAACATGGGGCTGGGTAAAGGGTCACAGTGGTAATGCTGGCAATGAAAAGGCGGATGAGTTAGCAAACAAAGCTATTGC
TGAGCTAATAGGGAAATAAA

Downstream 100 bases:

>100_bases
CTTAAATATAAATAGTAAAAAGCTTATACTTATAGCTAGCTAATACTAATAATAATTTCAAGGAGATAAAATGAGTGATA
TTTCTGAGTTGATAAAAGGC

Product: L-asparaginase

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKDLENRANKKILVLYTGGTIGMVSTEQGYDVKPGYLSETIAGIRDFYHYNMPQFEIKEYSELIDSSNINPRKWLSLALD
IIKTYEDYDGFVILHGTDTLAYTASVLSFMLGELQKPVIVTGSQIPISKIRSDAISNILNSLIFACSDDIKEVCVYFNQK
LMRGNRTTKISATDFDAFASPNYPALARVGIDIVVRQEKLWQRADTFSQPKLETFAVPKIAILSVFPGMGNDILEAVLEP
PLQGLILKTYGAGNMINDPSIHMTLKKTNDRGVVIVNCTQCLYGSVKMDAYKAARGLVEAGVVSGYDMTDEAALGKLFYL
LSQPNITQQDVKEAFNVSLQGEVTI

Sequences:

>Translated_345_residues
MKDLENRANKKILVLYTGGTIGMVSTEQGYDVKPGYLSETIAGIRDFYHYNMPQFEIKEYSELIDSSNINPRKWLSLALD
IIKTYEDYDGFVILHGTDTLAYTASVLSFMLGELQKPVIVTGSQIPISKIRSDAISNILNSLIFACSDDIKEVCVYFNQK
LMRGNRTTKISATDFDAFASPNYPALARVGIDIVVRQEKLWQRADTFSQPKLETFAVPKIAILSVFPGMGNDILEAVLEP
PLQGLILKTYGAGNMINDPSIHMTLKKTNDRGVVIVNCTQCLYGSVKMDAYKAARGLVEAGVVSGYDMTDEAALGKLFYL
LSQPNITQQDVKEAFNVSLQGEVTI
>Mature_345_residues
MKDLENRANKKILVLYTGGTIGMVSTEQGYDVKPGYLSETIAGIRDFYHYNMPQFEIKEYSELIDSSNINPRKWLSLALD
IIKTYEDYDGFVILHGTDTLAYTASVLSFMLGELQKPVIVTGSQIPISKIRSDAISNILNSLIFACSDDIKEVCVYFNQK
LMRGNRTTKISATDFDAFASPNYPALARVGIDIVVRQEKLWQRADTFSQPKLETFAVPKIAILSVFPGMGNDILEAVLEP
PLQGLILKTYGAGNMINDPSIHMTLKKTNDRGVVIVNCTQCLYGSVKMDAYKAARGLVEAGVVSGYDMTDEAALGKLFYL
LSQPNITQQDVKEAFNVSLQGEVTI

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=358, Percent_Identity=40.5027932960894, Blast_Score=249, Evalue=3e-66,
Organism=Escherichia coli, GI1788065, Length=341, Percent_Identity=46.9208211143695, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1789327, Length=248, Percent_Identity=27.8225806451613, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17558280, Length=360, Percent_Identity=42.7777777777778, Blast_Score=276, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI17558278, Length=360, Percent_Identity=42.7777777777778, Blast_Score=276, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI193207372, Length=360, Percent_Identity=42.7777777777778, Blast_Score=276, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI193207374, Length=360, Percent_Identity=42.7777777777778, Blast_Score=276, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24639609, Length=366, Percent_Identity=39.8907103825137, Blast_Score=257, Evalue=7e-69,
Organism=Drosophila melanogaster, GI24645530, Length=357, Percent_Identity=38.0952380952381, Blast_Score=245, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 38217; Mature: 38217

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLENRANKKILVLYTGGTIGMVSTEQGYDVKPGYLSETIAGIRDFYHYNMPQFEIKEY
CCCCHHHCCCEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
SELIDSSNINPRKWLSLALDIIKTYEDYDGFVILHGTDTLAYTASVLSFMLGELQKPVIV
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEE
TGSQIPISKIRSDAISNILNSLIFACSDDIKEVCVYFNQKLMRGNRTTKISATDFDAFAS
ECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEECCCCHHCCC
PNYPALARVGIDIVVRQEKLWQRADTFSQPKLETFAVPKIAILSVFPGMGNDILEAVLEP
CCCCEEHHCCEEEEEEHHHHHHHHCCCCCCCCHHHCCCCEEEEECCCCCCHHHHHHHHCC
PLQGLILKTYGAGNMINDPSIHMTLKKTNDRGVVIVNCTQCLYGSVKMDAYKAARGLVEA
CCCCEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHH
GVVSGYDMTDEAALGKLFYLLSQPNITQQDVKEAFNVSLQGEVTI
HCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEC
>Mature Secondary Structure
MKDLENRANKKILVLYTGGTIGMVSTEQGYDVKPGYLSETIAGIRDFYHYNMPQFEIKEY
CCCCHHHCCCEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
SELIDSSNINPRKWLSLALDIIKTYEDYDGFVILHGTDTLAYTASVLSFMLGELQKPVIV
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEE
TGSQIPISKIRSDAISNILNSLIFACSDDIKEVCVYFNQKLMRGNRTTKISATDFDAFAS
ECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEECCCCHHCCC
PNYPALARVGIDIVVRQEKLWQRADTFSQPKLETFAVPKIAILSVFPGMGNDILEAVLEP
CCCCEEHHCCEEEEEEHHHHHHHHCCCCCCCCHHHCCCCEEEEECCCCCCHHHHHHHHCC
PLQGLILKTYGAGNMINDPSIHMTLKKTNDRGVVIVNCTQCLYGSVKMDAYKAARGLVEA
CCCCEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHH
GVVSGYDMTDEAALGKLFYLLSQPNITQQDVKEAFNVSLQGEVTI
HCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]