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The map label for this gene is miaA

Identifier: 134302064

GI number: 134302064

Start: 1040347

End: 1041273

Strand: Reverse

Name: miaA

Synonym: FTW_1100

Alternate gene names: 134302064

Gene position: 1041273-1040347 (Counterclockwise)

Preceding gene: 134302065

Following gene: 134302063

Centisome position: 54.85

GC content: 33.01

Gene sequence:

>927_bases
ATGAGCAAATTAATCTATGGTTTGGCTGGACCAACAGCTTCTGGTAAAACTTCATTATCAATATTGTTAGCTAAAAAAAT
TAATGCAGAAATTATTAGTGTTGACTCATCTCTTGTTTATAAAGGTATGGATATTGGTACAGCCAAGCCAACTTTACAAG
AGCAAGATGGTATTAAGCATCATCTTATAGATATTATTGAGCCAACAGGGAATTTTTCGGTTGCTGACTTTATATCCAGT
GTTAATAAACTCAAAAAAGAAATTTGGGCTAGAGGAAGAGAAGTCTTACTTGTTGGTGGGACAATGCTCTATTTTAAAGG
TTTGATAGAGGGTTTATCTGCGTTACCAGAATCTCAAGCCGAGATTAGAGAAGCACTAGAATACCAGAAAAAAGCTAAAG
GATTACAATATCTTCATCAGCAACTAAATGAAATTGATCCACAATCTGCTCAAAAAATTAACCCTAATGACCAGCAAAGA
ATCTTTCGAGCTTTAGAGGTGATTATGATAAGTGGAAAAAAATACTCTGAGCTTGTTAAAACATCTAAAGTTGGTGGTTT
GGATGAAGATCTAAAATTATGTGCTTTAGTGCCAAATGATAGATCAATATTGCATAAAAATATTGAGTCTAGATTTAGAC
AAATGCTTGATCAAGGATTTTTGGATGAAGTCCAAAATTTACACAAAAATCCTATGCTTACTAAAGAAACCACAGCTATT
AGAAGTGTTGGATATCGTCAAGCATGGGAATATCTTGATGGTGATATTAGTTATGATGAGTTTGTTAAAAAAGGAATTGT
AGCCACTCGCCAACTTGCTAAGCGTCAACTAACATGGATTCGTAATTGGCAGAGTTCTATAAATATAGTTGCTATGGAGA
ACGAAACTAAAGAGTTAGATATTTTAAAATATTTTGGTTATAAATAA

Upstream 100 bases:

>100_bases
TAGTCAAGTTATTGATGCGTATAAACAAGAAGAATTAAACTTTTATTTACAAGTAATTAAACAACAAATTCCAATTCAAG
TTAATTATAAAAATATTTAG

Downstream 100 bases:

>100_bases
GATATAAGATTTGCTGATAGATATAAATCTTGCTAGAATATACGCGAAGTTTAATTGTTAAACTTGATAAAATAATAACT
ATAACAAAATAATTAAAAGG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSKLIYGLAGPTASGKTSLSILLAKKINAEIISVDSSLVYKGMDIGTAKPTLQEQDGIKHHLIDIIEPTGNFSVADFISS
VNKLKKEIWARGREVLLVGGTMLYFKGLIEGLSALPESQAEIREALEYQKKAKGLQYLHQQLNEIDPQSAQKINPNDQQR
IFRALEVIMISGKKYSELVKTSKVGGLDEDLKLCALVPNDRSILHKNIESRFRQMLDQGFLDEVQNLHKNPMLTKETTAI
RSVGYRQAWEYLDGDISYDEFVKKGIVATRQLAKRQLTWIRNWQSSINIVAMENETKELDILKYFGYK

Sequences:

>Translated_308_residues
MSKLIYGLAGPTASGKTSLSILLAKKINAEIISVDSSLVYKGMDIGTAKPTLQEQDGIKHHLIDIIEPTGNFSVADFISS
VNKLKKEIWARGREVLLVGGTMLYFKGLIEGLSALPESQAEIREALEYQKKAKGLQYLHQQLNEIDPQSAQKINPNDQQR
IFRALEVIMISGKKYSELVKTSKVGGLDEDLKLCALVPNDRSILHKNIESRFRQMLDQGFLDEVQNLHKNPMLTKETTAI
RSVGYRQAWEYLDGDISYDEFVKKGIVATRQLAKRQLTWIRNWQSSINIVAMENETKELDILKYFGYK
>Mature_307_residues
SKLIYGLAGPTASGKTSLSILLAKKINAEIISVDSSLVYKGMDIGTAKPTLQEQDGIKHHLIDIIEPTGNFSVADFISSV
NKLKKEIWARGREVLLVGGTMLYFKGLIEGLSALPESQAEIREALEYQKKAKGLQYLHQQLNEIDPQSAQKINPNDQQRI
FRALEVIMISGKKYSELVKTSKVGGLDEDLKLCALVPNDRSILHKNIESRFRQMLDQGFLDEVQNLHKNPMLTKETTAIR
SVGYRQAWEYLDGDISYDEFVKKGIVATRQLAKRQLTWIRNWQSSINIVAMENETKELDILKYFGYK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=304, Percent_Identity=29.9342105263158, Blast_Score=130, Evalue=1e-30,
Organism=Escherichia coli, GI1790613, Length=306, Percent_Identity=49.0196078431373, Blast_Score=288, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI25144712, Length=295, Percent_Identity=30.1694915254237, Blast_Score=101, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324848, Length=293, Percent_Identity=29.6928327645051, Blast_Score=120, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24649830, Length=268, Percent_Identity=31.7164179104478, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_FRAT1 (Q14IJ4)

Other databases:

- EMBL:   AM286280
- RefSeq:   YP_666782.1
- ProteinModelPortal:   Q14IJ4
- SMR:   Q14IJ4
- STRING:   Q14IJ4
- GeneID:   4199844
- GenomeReviews:   AM286280_GR
- KEGG:   ftf:FTF0629
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q14IJ4
- ProtClustDB:   PRK00091
- BioCyc:   FTUL393115:FTF0629-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34834; Mature: 34702

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLIYGLAGPTASGKTSLSILLAKKINAEIISVDSSLVYKGMDIGTAKPTLQEQDGIKH
CCCCEEECCCCCCCCCCHHHHHHHHHCCCEEEEECHHHHHCCCCCCCCCCCCCHHCCHHH
HLIDIIEPTGNFSVADFISSVNKLKKEIWARGREVLLVGGTMLYFKGLIEGLSALPESQA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCHH
EIREALEYQKKAKGLQYLHQQLNEIDPQSAQKINPNDQQRIFRALEVIMISGKKYSELVK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
TSKVGGLDEDLKLCALVPNDRSILHKNIESRFRQMLDQGFLDEVQNLHKNPMLTKETTAI
HHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RSVGYRQAWEYLDGDISYDEFVKKGIVATRQLAKRQLTWIRNWQSSINIVAMENETKELD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHH
ILKYFGYK
HHHHCCCC
>Mature Secondary Structure 
SKLIYGLAGPTASGKTSLSILLAKKINAEIISVDSSLVYKGMDIGTAKPTLQEQDGIKH
CCCEEECCCCCCCCCCHHHHHHHHHCCCEEEEECHHHHHCCCCCCCCCCCCCHHCCHHH
HLIDIIEPTGNFSVADFISSVNKLKKEIWARGREVLLVGGTMLYFKGLIEGLSALPESQA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCHH
EIREALEYQKKAKGLQYLHQQLNEIDPQSAQKINPNDQQRIFRALEVIMISGKKYSELVK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
TSKVGGLDEDLKLCALVPNDRSILHKNIESRFRQMLDQGFLDEVQNLHKNPMLTKETTAI
HHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RSVGYRQAWEYLDGDISYDEFVKKGIVATRQLAKRQLTWIRNWQSSINIVAMENETKELD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHH
ILKYFGYK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA