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The map label for this gene is pilD [H]

Identifier: 134302013

GI number: 134302013

Start: 989293

End: 990084

Strand: Direct

Name: pilD [H]

Synonym: FTW_1045

Alternate gene names: 134302013

Gene position: 989293-990084 (Clockwise)

Preceding gene: 134302012

Following gene: 134302014

Centisome position: 52.11

GC content: 32.83

Gene sequence:

>792_bases
TTGTTTATATTTCTATTTGGTGCTGCTATTGGCAGTTTTTTGAATGTTGTTATATATCGAGTGCCAAATAAACTATTTGC
TGATGAGCAAGCTATTGCACGAGAAATACTGGGGATTGATAAGCAACAATCTCCACAGAATTTTAGTTTATTGACACCAT
CAAAATGCCCAAAATGTCATAATAAACTAAAGTATCGTCATAATATTCCGATAATCGGATGGTTTTTGCTTAAAGGCAAA
TGCTTTTTCTGTAAAGAAAAAATATCTTTTGAATACCCATTAATAGAGTTTATCACAGCTAGTTTATTTATAACTATTTT
TTACTGTTTTGGTTTTACATTTCAGAGTTTAGCTCTAGTAACTTTGGCTAGCTTTTTTATACCATTATTCTTTATTGATG
CCAAACATCAAATCCTTCCTGATTCGTTAACACTACCATTACTATGGCTTGGGATAATACTTAATTACTACCATACGTTT
ACTACCCTAGAGCAATCTGTCTGGGGAGCTATAATTGGCTATCTTTCACTTTGGTTGGTTTTTTGGATATACAAAATCCT
TACAGGTAAAGAAGGCTTTGGTCATGGTGATTTTAAACTCTTAGCTGCAGTGGGTGCTTGGTTTGGCTATCCAATGTTAC
TGTATACAATTTTCGCTAGTTGTATATTTGGGATTATAATCGCTATTGCTATAAATCTTGTTGCTAAGCGTACTAATGTA
ATAGCTTTTGGGCCCGCGATAATTCTAGCAACATTTTTTTATCTACTGACTAAAGATAATATCTATGTATAG

Upstream 100 bases:

>100_bases
TATTGCTGGTATTGACGGTCTTAATAAGCTTAAACCTAAAAATAAACAATTTGTCCCAGAACACCAATAATCCATGTACT
ACGATATCTATATAATTTAC

Downstream 100 bases:

>100_bases
TATAATCACGTAATGCTAATTCAATCTTAATAATAACTAATGCAAAAAAAAAGCTTCTCTATTCTAGTTTTATTTGTTGT
TATACTATTAGTTGTACTGT

Product: prepilin peptidase family protein

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MFIFLFGAAIGSFLNVVIYRVPNKLFADEQAIAREILGIDKQQSPQNFSLLTPSKCPKCHNKLKYRHNIPIIGWFLLKGK
CFFCKEKISFEYPLIEFITASLFITIFYCFGFTFQSLALVTLASFFIPLFFIDAKHQILPDSLTLPLLWLGIILNYYHTF
TTLEQSVWGAIIGYLSLWLVFWIYKILTGKEGFGHGDFKLLAAVGAWFGYPMLLYTIFASCIFGIIIAIAINLVAKRTNV
IAFGPAIILATFFYLLTKDNIYV

Sequences:

>Translated_263_residues
MFIFLFGAAIGSFLNVVIYRVPNKLFADEQAIAREILGIDKQQSPQNFSLLTPSKCPKCHNKLKYRHNIPIIGWFLLKGK
CFFCKEKISFEYPLIEFITASLFITIFYCFGFTFQSLALVTLASFFIPLFFIDAKHQILPDSLTLPLLWLGIILNYYHTF
TTLEQSVWGAIIGYLSLWLVFWIYKILTGKEGFGHGDFKLLAAVGAWFGYPMLLYTIFASCIFGIIIAIAINLVAKRTNV
IAFGPAIILATFFYLLTKDNIYV
>Mature_263_residues
MFIFLFGAAIGSFLNVVIYRVPNKLFADEQAIAREILGIDKQQSPQNFSLLTPSKCPKCHNKLKYRHNIPIIGWFLLKGK
CFFCKEKISFEYPLIEFITASLFITIFYCFGFTFQSLALVTLASFFIPLFFIDAKHQILPDSLTLPLLWLGIILNYYHTF
TTLEQSVWGAIIGYLSLWLVFWIYKILTGKEGFGHGDFKLLAAVGAWFGYPMLLYTIFASCIFGIIIAIAINLVAKRTNV
IAFGPAIILATFFYLLTKDNIYV

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=246, Percent_Identity=35.3658536585366, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1789732, Length=164, Percent_Identity=39.6341463414634, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 29989; Mature: 29989

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIFLFGAAIGSFLNVVIYRVPNKLFADEQAIAREILGIDKQQSPQNFSLLTPSKCPKCH
CEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
NKLKYRHNIPIIGWFLLKGKCFFCKEKISFEYPLIEFITASLFITIFYCFGFTFQSLALV
HHHHHHCCCCEEEHHHHHCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLASFFIPLFFIDAKHQILPDSLTLPLLWLGIILNYYHTFTTLEQSVWGAIIGYLSLWLV
HHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FWIYKILTGKEGFGHGDFKLLAAVGAWFGYPMLLYTIFASCIFGIIIAIAINLVAKRTNV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
IAFGPAIILATFFYLLTKDNIYV
EEEHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure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EEEHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8100347; 7906688; 7565116 [H]