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The map label for this gene is rlmN [H]

Identifier: 134301956

GI number: 134301956

Start: 928590

End: 929702

Strand: Reverse

Name: rlmN [H]

Synonym: FTW_0968

Alternate gene names: 134301956

Gene position: 929702-928590 (Counterclockwise)

Preceding gene: 134301957

Following gene: 134301955

Centisome position: 48.97

GC content: 34.86

Gene sequence:

>1113_bases
ATGCAACAAGATAAAGTTAATTTACTTGGATTAAATCAAAAAGCTATAGAAGATTTTTTTATTTCTATAGGCGAAAAAAA
ATTTCATGCAAGACAAGTTTTTAAGTGGATTCATAAAAAAGGTGTTATTGACTTTGATGCTATGACAGACCTTGGCAAAA
ATTTACGCCATAAACTTAAGGAAAAAGCTCAGATAACTATTCCTAAAGTGGTATTTAGTAAGGCATCAAAAGATGGTACT
CATAAGTGGCTTATCGATGTTGGTGGTAGTGCAGTTGAGACAGTATTTATTCTAGAGGAAGGTAGAGGAACATTATGTGT
ATCTTCACAGGTTGGCTGTACTTTAAACTGTAGTTTTTGCTCTACTGGTAAACAAGGCTTCAATAGAAATCTATCAGCAG
CTGAAGTTATCGCACAGTTATGGATAGCAGCTAGAACATTATCAAAAACTGATGGCGAACATGATTTCACAGTAACAAAT
ATTGTTATGATGGGTATGGGTGAGCCGTTGATGAATTTTGAGAATGTAGTTCCAGCAATGGATATTATGATGGATGACTT
AGCTTATGGATTGTCTCGACGTAAAGTAACTCTAAGTACATCTGGTGTGGTTCCGAGAATATATGACTTATTAGAACAAT
CAGGAGTATCTTTAGCTGTATCTTTACATGCGCCAAATGATATGTTGCGTAATGAAATTGTACCTATTAACAAGAAGTAT
AATATTGATGAGCTTCTTGAGGCATGTAAGCTATATGCACAAAAGGGTCCACATAAACACATTACTTTTGAATATACTTT
AATGGAAGAGGTTAATGATAATTTATCTGATGCTGAGGAATTAGTTGCGTTATTAAAATCACGCGAAGTTCCTGCTAAAA
TTAATCTTATCCCATTTAATCCATATCCAGGCACGCCTTATAAAAAGCCTAGCAATAACCGTATTCATAGATTTAAAGAG
TTTTTGCAGCACAATGGTTTTGTCACTACGGTGAGAAAAACTCGTGGGGATGATATTGATGCTGCTTGTGGCCAATTAGC
TGGCGATGTTATGGATAAAACTAATAGAAAGCAAAGATACCTCAAGAAGCTTGGAGACACTAATGCAAATTAG

Upstream 100 bases:

>100_bases
TGCTAGTTTTGCTAATTTAACTAATGAAAATGATCATATTTTACCTGGTGATATAGGTTATAACGAGTAATATTTATATA
AAGATTTAAAGAGAAGTTTC

Downstream 100 bases:

>100_bases
TTTAAAAAAAGTTATAAGTGTTGCGTTGTTATCGGCTGTCTTATCATCATGTATGACTCCAGAAACCCCTACTCGTAATT
CTATAAAAAGTAACTCGCAG

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MQQDKVNLLGLNQKAIEDFFISIGEKKFHARQVFKWIHKKGVIDFDAMTDLGKNLRHKLKEKAQITIPKVVFSKASKDGT
HKWLIDVGGSAVETVFILEEGRGTLCVSSQVGCTLNCSFCSTGKQGFNRNLSAAEVIAQLWIAARTLSKTDGEHDFTVTN
IVMMGMGEPLMNFENVVPAMDIMMDDLAYGLSRRKVTLSTSGVVPRIYDLLEQSGVSLAVSLHAPNDMLRNEIVPINKKY
NIDELLEACKLYAQKGPHKHITFEYTLMEEVNDNLSDAEELVALLKSREVPAKINLIPFNPYPGTPYKKPSNNRIHRFKE
FLQHNGFVTTVRKTRGDDIDAACGQLAGDVMDKTNRKQRYLKKLGDTNAN

Sequences:

>Translated_370_residues
MQQDKVNLLGLNQKAIEDFFISIGEKKFHARQVFKWIHKKGVIDFDAMTDLGKNLRHKLKEKAQITIPKVVFSKASKDGT
HKWLIDVGGSAVETVFILEEGRGTLCVSSQVGCTLNCSFCSTGKQGFNRNLSAAEVIAQLWIAARTLSKTDGEHDFTVTN
IVMMGMGEPLMNFENVVPAMDIMMDDLAYGLSRRKVTLSTSGVVPRIYDLLEQSGVSLAVSLHAPNDMLRNEIVPINKKY
NIDELLEACKLYAQKGPHKHITFEYTLMEEVNDNLSDAEELVALLKSREVPAKINLIPFNPYPGTPYKKPSNNRIHRFKE
FLQHNGFVTTVRKTRGDDIDAACGQLAGDVMDKTNRKQRYLKKLGDTNAN
>Mature_370_residues
MQQDKVNLLGLNQKAIEDFFISIGEKKFHARQVFKWIHKKGVIDFDAMTDLGKNLRHKLKEKAQITIPKVVFSKASKDGT
HKWLIDVGGSAVETVFILEEGRGTLCVSSQVGCTLNCSFCSTGKQGFNRNLSAAEVIAQLWIAARTLSKTDGEHDFTVTN
IVMMGMGEPLMNFENVVPAMDIMMDDLAYGLSRRKVTLSTSGVVPRIYDLLEQSGVSLAVSLHAPNDMLRNEIVPINKKY
NIDELLEACKLYAQKGPHKHITFEYTLMEEVNDNLSDAEELVALLKSREVPAKINLIPFNPYPGTPYKKPSNNRIHRFKE
FLQHNGFVTTVRKTRGDDIDAACGQLAGDVMDKTNRKQRYLKKLGDTNAN

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=357, Percent_Identity=56.0224089635854, Blast_Score=410, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41478; Mature: 41478

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQDKVNLLGLNQKAIEDFFISIGEKKFHARQVFKWIHKKGVIDFDAMTDLGKNLRHKLK
CCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EKAQITIPKVVFSKASKDGTHKWLIDVGGSAVETVFILEEGRGTLCVSSQVGCTLNCSFC
HHHCEEHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEEECCCCEEEEECCCCEEEEEHHH
STGKQGFNRNLSAAEVIAQLWIAARTLSKTDGEHDFTVTNIVMMGMGEPLMNFENVVPAM
CCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHEECCCCHHHHHHHHHHHH
DIMMDDLAYGLSRRKVTLSTSGVVPRIYDLLEQSGVSLAVSLHAPNDMLRNEIVPINKKY
HHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEECCHHHHHCCCCCCCCCC
NIDELLEACKLYAQKGPHKHITFEYTLMEEVNDNLSDAEELVALLKSREVPAKINLIPFN
CHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECC
PYPGTPYKKPSNNRIHRFKEFLQHNGFVTTVRKTRGDDIDAACGQLAGDVMDKTNRKQRY
CCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHH
LKKLGDTNAN
HHHHCCCCCC
>Mature Secondary Structure
MQQDKVNLLGLNQKAIEDFFISIGEKKFHARQVFKWIHKKGVIDFDAMTDLGKNLRHKLK
CCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EKAQITIPKVVFSKASKDGTHKWLIDVGGSAVETVFILEEGRGTLCVSSQVGCTLNCSFC
HHHCEEHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEEECCCCEEEEECCCCEEEEEHHH
STGKQGFNRNLSAAEVIAQLWIAARTLSKTDGEHDFTVTNIVMMGMGEPLMNFENVVPAM
CCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHEECCCCHHHHHHHHHHHH
DIMMDDLAYGLSRRKVTLSTSGVVPRIYDLLEQSGVSLAVSLHAPNDMLRNEIVPINKKY
HHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEECCHHHHHCCCCCCCCCC
NIDELLEACKLYAQKGPHKHITFEYTLMEEVNDNLSDAEELVALLKSREVPAKINLIPFN
CHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECC
PYPGTPYKKPSNNRIHRFKEFLQHNGFVTTVRKTRGDDIDAACGQLAGDVMDKTNRKQRY
CCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHH
LKKLGDTNAN
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA