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The map label for this gene is rluB [H]

Identifier: 134301954

GI number: 134301954

Start: 926887

End: 927702

Strand: Reverse

Name: rluB [H]

Synonym: FTW_0966

Alternate gene names: 134301954

Gene position: 927702-926887 (Counterclockwise)

Preceding gene: 134301955

Following gene: 134301953

Centisome position: 48.87

GC content: 34.8

Gene sequence:

>816_bases
ATGCGTAGAGCAAATCAAAATAATGATAAAAATCCTGAAAGATTACAAAAGCTTTTAGCAAAATATGGTATTGGATCTCG
AAGAAAAATAGAAGAGTATATAGAGCAAGGTAGAGTTAAGGTAAATGGTAAAGTCGCTACGCTAGGAGATAAAGCTATTG
AAGCTGACAAAATCAGTTTCGATGGTAAAGCTCTACACTCATATGGTCAGCCAATGACTAGACCACGAGTAGTCATTTAT
CATAAAAGAGAAGGTGAAGTTTGTACTAGCAAAGATGAGAAAGACCGCCAAACAGTCTTTGATTCATTACCAAAATTAGC
AAAGTCACGTTGGATAATGGTAGGACGCTTAGATATTAATACTACAGGTTTGTTGCTTTTTACTACAGATGGTGATTTGG
CAAATAGATTGATGCATCCTTCTTACCAAATTGAGAGAGAGTATGCAGTGCGTGTATTTGGTCAACAATTATCTGATGAA
ACTATAAATAAGCTTAAAGAGGGCATACAGCTAGAGGATGGTATGGCTAAGTTTAATAGTATAAAATTCTCAGGTGGTGA
GGGTGCTAATCTTTGGTATTATGTGACACTTTCTGAAGGACGTAATCGAGAAGTAAGAAGAATGTTTGAAGCAGTAGGTG
TTACTGTTAGTCGACTAACGAGAATTAGATTTGGTGATATAATCCTTCCTAAATTTGTTTCGCGTGGCAAAACATTAGAA
CTAAATCCATCAGAAGTTAATCGACTTAGAAAGTCTGTTAAGTTAAAAGAGTATAGTTTTCCTAAGAAATTAGTTGAAAG
GTTAGAGAAAAAATAA

Upstream 100 bases:

>100_bases
CAAGCTGCTGAAGTAAGACTAATTTTATCTTCTAATAACTATAATGACGAAGATATCCAAAAATTTTTCTCAGCAGCCGA
TCAAGAGGATATAAATAAAA

Downstream 100 bases:

>100_bases
TGAGCTTTGCAAGCCAGATAGAAGAAATTTTTGGTGTAGATATTTGGGAATTAAAACCACAATATAAAACTTCGCAACAA
AATCAAACTACTAATGATGC

Product: putative RNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MRRANQNNDKNPERLQKLLAKYGIGSRRKIEEYIEQGRVKVNGKVATLGDKAIEADKISFDGKALHSYGQPMTRPRVVIY
HKREGEVCTSKDEKDRQTVFDSLPKLAKSRWIMVGRLDINTTGLLLFTTDGDLANRLMHPSYQIEREYAVRVFGQQLSDE
TINKLKEGIQLEDGMAKFNSIKFSGGEGANLWYYVTLSEGRNREVRRMFEAVGVTVSRLTRIRFGDIILPKFVSRGKTLE
LNPSEVNRLRKSVKLKEYSFPKKLVERLEKK

Sequences:

>Translated_271_residues
MRRANQNNDKNPERLQKLLAKYGIGSRRKIEEYIEQGRVKVNGKVATLGDKAIEADKISFDGKALHSYGQPMTRPRVVIY
HKREGEVCTSKDEKDRQTVFDSLPKLAKSRWIMVGRLDINTTGLLLFTTDGDLANRLMHPSYQIEREYAVRVFGQQLSDE
TINKLKEGIQLEDGMAKFNSIKFSGGEGANLWYYVTLSEGRNREVRRMFEAVGVTVSRLTRIRFGDIILPKFVSRGKTLE
LNPSEVNRLRKSVKLKEYSFPKKLVERLEKK
>Mature_271_residues
MRRANQNNDKNPERLQKLLAKYGIGSRRKIEEYIEQGRVKVNGKVATLGDKAIEADKISFDGKALHSYGQPMTRPRVVIY
HKREGEVCTSKDEKDRQTVFDSLPKLAKSRWIMVGRLDINTTGLLLFTTDGDLANRLMHPSYQIEREYAVRVFGQQLSDE
TINKLKEGIQLEDGMAKFNSIKFSGGEGANLWYYVTLSEGRNREVRRMFEAVGVTVSRLTRIRFGDIILPKFVSRGKTLE
LNPSEVNRLRKSVKLKEYSFPKKLVERLEKK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=257, Percent_Identity=57.9766536964981, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1788510, Length=237, Percent_Identity=32.0675105485232, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790453, Length=219, Percent_Identity=32.4200913242009, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=31.638418079096, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 31186; Mature: 31186

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRANQNNDKNPERLQKLLAKYGIGSRRKIEEYIEQGRVKVNGKVATLGDKAIEADKISF
CCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCEEEECCCCHHCCCCCCC
DGKALHSYGQPMTRPRVVIYHKREGEVCTSKDEKDRQTVFDSLPKLAKSRWIMVGRLDIN
CCHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEC
TTGLLLFTTDGDLANRLMHPSYQIEREYAVRVFGQQLSDETINKLKEGIQLEDGMAKFNS
CCEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHE
IKFSGGEGANLWYYVTLSEGRNREVRRMFEAVGVTVSRLTRIRFGDIILPKFVSRGKTLE
EEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEE
LNPSEVNRLRKSVKLKEYSFPKKLVERLEKK
ECHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MRRANQNNDKNPERLQKLLAKYGIGSRRKIEEYIEQGRVKVNGKVATLGDKAIEADKISF
CCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCEEEECCCCHHCCCCCCC
DGKALHSYGQPMTRPRVVIYHKREGEVCTSKDEKDRQTVFDSLPKLAKSRWIMVGRLDIN
CCHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEC
TTGLLLFTTDGDLANRLMHPSYQIEREYAVRVFGQQLSDETINKLKEGIQLEDGMAKFNS
CCEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHE
IKFSGGEGANLWYYVTLSEGRNREVRRMFEAVGVTVSRLTRIRFGDIILPKFVSRGKTLE
EEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEE
LNPSEVNRLRKSVKLKEYSFPKKLVERLEKK
ECHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]