Click here to switch to the map view.

The map label for this gene is ispB [H]

Identifier: 134301942

GI number: 134301942

Start: 916687

End: 917664

Strand: Reverse

Name: ispB [H]

Synonym: FTW_0953

Alternate gene names: 134301942

Gene position: 917664-916687 (Counterclockwise)

Preceding gene: 134301943

Following gene: 134301936

Centisome position: 48.34

GC content: 32.11

Gene sequence:

>978_bases
ATGCAACAGCTAAAAGATATTCAGGCGCTTATTAAAGAGGATATACAAAATAATAATCAATTCATAGTTGATTCTCTTTG
CTCAGATGTCGTACTTATCAATCAGATTAGTCACTATATAATAAATAGTGGTGGTAAAAGATTAAGACCTCTGTTAGTAA
TGTTATTTGCTAGAGCCTTAAACTATAATGGAGATAAACATTTAGCATGTGCTGCAATTATTGAGTTTATTCATACAGCA
ACTCTTCTTCATGATGATGTTGTTGATGATTCGCAATTGCGTCGTGGTAAACAAACAGCAAATAATGTCTTTGGTAATGC
TGCGAGTGTTTTGACTGGAGATTTTCTCTATTCTAGAGCTTTTCAGATGATGGTAAGTTTAGATAATATGCAAATTATGC
AAATATTAGCTGATGCAACTAATAAAATCTCAGAAGGAGAGGTGCTACAACTACTAAATGCGCGTAATAGCGAACTTAGT
GAAGAAGAATATATTAAAGTGATATATTGCAAAACAGCAAAATTATTTGAAGCTGCTTGTGAATTAGCAGGTGTAATTAG
TCTAGATAAACAAGATTATACTAAGTACCAAGGTAGGATTAAAAACTATGGTGTTTATTTAGGTAATGCTTTTCAGATTG
CAGATGATGTTTTAGATTATGTATCTGATGCAGAAAGTTTAGGTAAAAATATTGGTGATGATCTTGATGAAGGCAAAATG
ACTTTGCCAACAATCTACGCACTAGCTAATGTTACTGAACAACAGCAACAAATTCTAAAAAAAGCTATTGAAAAGGGCGA
GTATAGAATTGATGAAATCATAGCTATGGTCAAAGATAGTGGTGCTGTAGATTATTCTTATAAAGTGGCTTGTCAGTATG
CTGATCTCGCTAAACAACAAATAGATTTTTTACCAGATTCAGAATATAAGCAAGCTATGATTTTATTATGTGATCTAGCA
GTAAATAGGAAGAATTAA

Upstream 100 bases:

>100_bases
AAGATAATTAGTTAGATTATTTAGTAATATGTATTGATAAATGTATTCTGCTACTTTATTCTTAATCCTAATTAAGTAAA
TACTATCAATAAAATAAATA

Downstream 100 bases:

>100_bases
TTTTTATAAAAAATTTTTTATTTAAAATCTTTAAGCGTAATTTCAAAAGAAAGTGCTTGATTAGGTCCTATAACAGCGGG
CGCTCTAGTTCCATATGCCT

Product: polyprenyl synthetase family protein

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MQQLKDIQALIKEDIQNNNQFIVDSLCSDVVLINQISHYIINSGGKRLRPLLVMLFARALNYNGDKHLACAAIIEFIHTA
TLLHDDVVDDSQLRRGKQTANNVFGNAASVLTGDFLYSRAFQMMVSLDNMQIMQILADATNKISEGEVLQLLNARNSELS
EEEYIKVIYCKTAKLFEAACELAGVISLDKQDYTKYQGRIKNYGVYLGNAFQIADDVLDYVSDAESLGKNIGDDLDEGKM
TLPTIYALANVTEQQQQILKKAIEKGEYRIDEIIAMVKDSGAVDYSYKVACQYADLAKQQIDFLPDSEYKQAMILLCDLA
VNRKN

Sequences:

>Translated_325_residues
MQQLKDIQALIKEDIQNNNQFIVDSLCSDVVLINQISHYIINSGGKRLRPLLVMLFARALNYNGDKHLACAAIIEFIHTA
TLLHDDVVDDSQLRRGKQTANNVFGNAASVLTGDFLYSRAFQMMVSLDNMQIMQILADATNKISEGEVLQLLNARNSELS
EEEYIKVIYCKTAKLFEAACELAGVISLDKQDYTKYQGRIKNYGVYLGNAFQIADDVLDYVSDAESLGKNIGDDLDEGKM
TLPTIYALANVTEQQQQILKKAIEKGEYRIDEIIAMVKDSGAVDYSYKVACQYADLAKQQIDFLPDSEYKQAMILLCDLA
VNRKN
>Mature_325_residues
MQQLKDIQALIKEDIQNNNQFIVDSLCSDVVLINQISHYIINSGGKRLRPLLVMLFARALNYNGDKHLACAAIIEFIHTA
TLLHDDVVDDSQLRRGKQTANNVFGNAASVLTGDFLYSRAFQMMVSLDNMQIMQILADATNKISEGEVLQLLNARNSELS
EEEYIKVIYCKTAKLFEAACELAGVISLDKQDYTKYQGRIKNYGVYLGNAFQIADDVLDYVSDAESLGKNIGDDLDEGKM
TLPTIYALANVTEQQQQILKKAIEKGEYRIDEIIAMVKDSGAVDYSYKVACQYADLAKQQIDFLPDSEYKQAMILLCDLA
VNRKN

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=300, Percent_Identity=32.6666666666667, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI4758430, Length=267, Percent_Identity=28.4644194756554, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI83700220, Length=267, Percent_Identity=28.4644194756554, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=47.8260869565217, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI1786623, Length=273, Percent_Identity=28.9377289377289, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17505681, Length=322, Percent_Identity=29.8136645962733, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319475, Length=299, Percent_Identity=28.7625418060201, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6325188, Length=294, Percent_Identity=24.8299319727891, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24651612, Length=301, Percent_Identity=29.2358803986711, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI281365769, Length=265, Percent_Identity=27.1698113207547, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24660002, Length=265, Percent_Identity=27.1698113207547, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36454; Mature: 36454

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQLKDIQALIKEDIQNNNQFIVDSLCSDVVLINQISHYIINSGGKRLRPLLVMLFARAL
CCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NYNGDKHLACAAIIEFIHTATLLHDDVVDDSQLRRGKQTANNVFGNAASVLTGDFLYSRA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FQMMVSLDNMQIMQILADATNKISEGEVLQLLNARNSELSEEEYIKVIYCKTAKLFEAAC
HHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHCEEEEEECHHHHHHHHH
ELAGVISLDKQDYTKYQGRIKNYGVYLGNAFQIADDVLDYVSDAESLGKNIGDDLDEGKM
HHHHHHCCCHHHHHHHHCCHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TLPTIYALANVTEQQQQILKKAIEKGEYRIDEIIAMVKDSGAVDYSYKVACQYADLAKQQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEHHEEHHHHHHHHHH
IDFLPDSEYKQAMILLCDLAVNRKN
HHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQQLKDIQALIKEDIQNNNQFIVDSLCSDVVLINQISHYIINSGGKRLRPLLVMLFARAL
CCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NYNGDKHLACAAIIEFIHTATLLHDDVVDDSQLRRGKQTANNVFGNAASVLTGDFLYSRA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FQMMVSLDNMQIMQILADATNKISEGEVLQLLNARNSELSEEEYIKVIYCKTAKLFEAAC
HHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHCEEEEEECHHHHHHHHH
ELAGVISLDKQDYTKYQGRIKNYGVYLGNAFQIADDVLDYVSDAESLGKNIGDDLDEGKM
HHHHHHCCCHHHHHHHHCCHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TLPTIYALANVTEQQQQILKKAIEKGEYRIDEIIAMVKDSGAVDYSYKVACQYADLAKQQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEHHEEHHHHHHHHHH
IDFLPDSEYKQAMILLCDLAVNRKN
HHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]