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The map label for this gene is xasA [C]

Identifier: 134301875

GI number: 134301875

Start: 840214

End: 841635

Strand: Reverse

Name: xasA [C]

Synonym: FTW_0870

Alternate gene names: 134301875

Gene position: 841635-840214 (Counterclockwise)

Preceding gene: 134301876

Following gene: 134301873

Centisome position: 44.33

GC content: 30.8

Gene sequence:

>1422_bases
ATGACTATGACAGTAAATTCATCAGCAAAAGAAAAAATTGGTTTAATTTTACTAGTATTGTTAATGACAGGAGCTATTGA
TAATATTAGAAATCTTCCATCTACAGCCACTTCAGGAACATATATATTCTTCTTTTTTGCAGTAGCTGTTTTTTTATTTT
TAGCACCAGTTGCATTAGTATCTGCAGAGATGACTACGACATATACTGCTAAAGGTGAAGAAGGAGTATATGGTTGGGTT
AAAAAAGCTTTTGGCCCTAATGTTGCGATGTTAGCTGTATGGTTCCAATGGATAAATACTCTTATCTGGTTTCCAAGTAT
TTTGACTTTCCTTGCAGGTACAATTGCATACTTATTTAATCCCGATTTTGCACAGAATATAAAATTTACAATTATATTTA
TAACTGTAGTTTTTTGGTCTTTGACAATTCTTAATCTTAAAGGTCTAAGAGTATCGGCAATTTTCGCTAGTACTTGTACT
TTTTTAGGTATGGTAATACCGATGTTATTAATGGTGCTATTTGCATTAATTTGGTTACTCAATATCTATGACCTTAATAT
TCATTTTCATCTAAACAATCTAATTCCTAGTTTTACATCTACAGATTCATGGATGGGACTAACAGCGATTATAGCTTCTT
TCTTAGGACTTGAGTTAGCAACCGTGCATATTAGAAAAGTTATAAATCCTAAAAAAACATTTCCTCTAGCACTTTTTATT
TCAGTAATATTTATAATCTTTACTATGGTTTTAGGTGCTTTAGCTGTTGCGATTATATTTCCACAGTCCCAAATTGATGT
TGTTCATGGTACTATCAAAACTTTTAAAGTTTATTTAGAGAGTTTGGGAATACCTGTATTTTTTTATTATATTTTGGGCT
TGATGGTTTTTGTTGGTTCAATAGGATCAATGATTAACTGGATGATTTCACCTGCAAGAGGATTACTTCAAGCAGCCGAT
GATCATTTTTTACCAAATATGTTAGATAAAACAAATAAGCATGATGTACCAAGTGACATATTAATCCTTCAGGCTATAAT
TATGACTATAATATGTCTGCTTTTAGAATTAGTGCCTTCAGTTCAAGCTTATTATTGGTTGCTTACAGCTCTTAGCACAC
AAATATATTCTTTGATGTATCTAATGATGTTTTTTGCCGCTTTAAAACTAAAACTAACAAATAACCAAACTGTAAGAAAT
ACCGATGATTTTCATATCCCTGGTGGGAAAATCGGTATGTCTATTGTTTGTATTTTAGGTATTATAGGCACTATTTTGTG
TGTGATCGTTGGCTTTGTCCCACCAGATAATTTGTATAGTAATCCGCTAGAGTTTATTGAAATGCTGTCTGTATGTTTTG
TATTATCAATTATACCTGTAGTATTCTTTATCATATATAGAAGAATTAAATTAAAAAATTAA

Upstream 100 bases:

>100_bases
TTAGAAAAACTTTTTCAAGTAATGCCATTATACATCGCATAAATCATTGCCAATTTAGATATCCTTGAGTTATTATATAT
CTTAAACTATCCACTAACGT

Downstream 100 bases:

>100_bases
TAAAATGTATGAATCATTAATACTTTTTCTAACTCTTGTTGTTGGCTATTACTTTTTCAATCTCAAAAGATTTCTCAAAA
TTATACGGTTGATAAATCGT

Product: amino acid antiporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTMTVNSSAKEKIGLILLVLLMTGAIDNIRNLPSTATSGTYIFFFFAVAVFLFLAPVALVSAEMTTTYTAKGEEGVYGWV
KKAFGPNVAMLAVWFQWINTLIWFPSILTFLAGTIAYLFNPDFAQNIKFTIIFITVVFWSLTILNLKGLRVSAIFASTCT
FLGMVIPMLLMVLFALIWLLNIYDLNIHFHLNNLIPSFTSTDSWMGLTAIIASFLGLELATVHIRKVINPKKTFPLALFI
SVIFIIFTMVLGALAVAIIFPQSQIDVVHGTIKTFKVYLESLGIPVFFYYILGLMVFVGSIGSMINWMISPARGLLQAAD
DHFLPNMLDKTNKHDVPSDILILQAIIMTIICLLLELVPSVQAYYWLLTALSTQIYSLMYLMMFFAALKLKLTNNQTVRN
TDDFHIPGGKIGMSIVCILGIIGTILCVIVGFVPPDNLYSNPLEFIEMLSVCFVLSIIPVVFFIIYRRIKLKN

Sequences:

>Translated_473_residues
MTMTVNSSAKEKIGLILLVLLMTGAIDNIRNLPSTATSGTYIFFFFAVAVFLFLAPVALVSAEMTTTYTAKGEEGVYGWV
KKAFGPNVAMLAVWFQWINTLIWFPSILTFLAGTIAYLFNPDFAQNIKFTIIFITVVFWSLTILNLKGLRVSAIFASTCT
FLGMVIPMLLMVLFALIWLLNIYDLNIHFHLNNLIPSFTSTDSWMGLTAIIASFLGLELATVHIRKVINPKKTFPLALFI
SVIFIIFTMVLGALAVAIIFPQSQIDVVHGTIKTFKVYLESLGIPVFFYYILGLMVFVGSIGSMINWMISPARGLLQAAD
DHFLPNMLDKTNKHDVPSDILILQAIIMTIICLLLELVPSVQAYYWLLTALSTQIYSLMYLMMFFAALKLKLTNNQTVRN
TDDFHIPGGKIGMSIVCILGIIGTILCVIVGFVPPDNLYSNPLEFIEMLSVCFVLSIIPVVFFIIYRRIKLKN
>Mature_472_residues
TMTVNSSAKEKIGLILLVLLMTGAIDNIRNLPSTATSGTYIFFFFAVAVFLFLAPVALVSAEMTTTYTAKGEEGVYGWVK
KAFGPNVAMLAVWFQWINTLIWFPSILTFLAGTIAYLFNPDFAQNIKFTIIFITVVFWSLTILNLKGLRVSAIFASTCTF
LGMVIPMLLMVLFALIWLLNIYDLNIHFHLNNLIPSFTSTDSWMGLTAIIASFLGLELATVHIRKVINPKKTFPLALFIS
VIFIIFTMVLGALAVAIIFPQSQIDVVHGTIKTFKVYLESLGIPVFFYYILGLMVFVGSIGSMINWMISPARGLLQAADD
HFLPNMLDKTNKHDVPSDILILQAIIMTIICLLLELVPSVQAYYWLLTALSTQIYSLMYLMMFFAALKLKLTNNQTVRNT
DDFHIPGGKIGMSIVCILGIIGTILCVIVGFVPPDNLYSNPLEFIEMLSVCFVLSIIPVVFFIIYRRIKLKN

Specific function: Probable Amino Acid Transporter. Involved In Extreme Acid Resistance. [C]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=447, Percent_Identity=25.9507829977629, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI2367353, Length=391, Percent_Identity=24.2966751918159, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87081798, Length=365, Percent_Identity=21.0958904109589, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87082380, Length=415, Percent_Identity=24.0963855421687, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786908, Length=244, Percent_Identity=25, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 52857; Mature: 52726

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMTVNSSAKEKIGLILLVLLMTGAIDNIRNLPSTATSGTYIFFFFAVAVFLFLAPVALV
CEEECCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SAEMTTTYTAKGEEGVYGWVKKAFGPNVAMLAVWFQWINTLIWFPSILTFLAGTIAYLFN
HHHHHEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDFAQNIKFTIIFITVVFWSLTILNLKGLRVSAIFASTCTFLGMVIPMLLMVLFALIWLL
CCHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIYDLNIHFHLNNLIPSFTSTDSWMGLTAIIASFLGLELATVHIRKVINPKKTFPLALFI
HHHHEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SVIFIIFTMVLGALAVAIIFPQSQIDVVHGTIKTFKVYLESLGIPVFFYYILGLMVFVGS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IGSMINWMISPARGLLQAADDHFLPNMLDKTNKHDVPSDILILQAIIMTIICLLLELVPS
HHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VQAYYWLLTALSTQIYSLMYLMMFFAALKLKLTNNQTVRNTDDFHIPGGKIGMSIVCILG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
IIGTILCVIVGFVPPDNLYSNPLEFIEMLSVCFVLSIIPVVFFIIYRRIKLKN
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TMTVNSSAKEKIGLILLVLLMTGAIDNIRNLPSTATSGTYIFFFFAVAVFLFLAPVALV
EEECCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SAEMTTTYTAKGEEGVYGWVKKAFGPNVAMLAVWFQWINTLIWFPSILTFLAGTIAYLFN
HHHHHEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDFAQNIKFTIIFITVVFWSLTILNLKGLRVSAIFASTCTFLGMVIPMLLMVLFALIWLL
CCHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIYDLNIHFHLNNLIPSFTSTDSWMGLTAIIASFLGLELATVHIRKVINPKKTFPLALFI
HHHHEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SVIFIIFTMVLGALAVAIIFPQSQIDVVHGTIKTFKVYLESLGIPVFFYYILGLMVFVGS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IGSMINWMISPARGLLQAADDHFLPNMLDKTNKHDVPSDILILQAIIMTIICLLLELVPS
HHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VQAYYWLLTALSTQIYSLMYLMMFFAALKLKLTNNQTVRNTDDFHIPGGKIGMSIVCILG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
IIGTILCVIVGFVPPDNLYSNPLEFIEMLSVCFVLSIIPVVFFIIYRRIKLKN
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8366052 [H]