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The map label for this gene is bioB [H]

Identifier: 134301848

GI number: 134301848

Start: 809235

End: 810176

Strand: Reverse

Name: bioB [H]

Synonym: FTW_0831

Alternate gene names: 134301848

Gene position: 810176-809235 (Counterclockwise)

Preceding gene: 134301850

Following gene: 134301847

Centisome position: 42.68

GC content: 31.32

Gene sequence:

>942_bases
ATGACATTACAACAAATCAAAGAAATTTACTCAAGACCATTAACAGAATTAATTTTACAAGCTTTAGAGATTCATAATAA
AAATTTTGGTAATGATATCGAGTTATGCTCACTAAAAAGTATCAAAACTGGTACTTGCCCAGAAGACTGTAAATATTGTC
CTCAAAGTGGTCATTATAATACTAGTATCGAAAAGCATAAACTATTAGATAAAGATAGTATATTGGCAGAAGCAAAAAAT
GCAAAAGATGCTGGATCTAAAAGGTTCTGTATGGGCGCTGCTTGGAAACACATTCCTAAAAAAGACTTTGATCAAGTTGC
TGAGATTATCACTGAAGTCAAAAACCTTGGCTTAGAAACATGTGTGACTCTCGGAAGTATTAATGCTGATGAAGCAACTA
AACTAAAGCAAGCAGGATTGGATTACTACAATCATAATCTTGATACTTCAAGAGAATTTTATCCGGAAATAATTACCACA
CGTAAATTTGAAGAAAGAATTGAAACTATTAGAAATGTTGCTAACGCCGATATAAATGTCTGTTGTGGTGGTATATTGGG
TATGGGCGAATCTCTAGATGATAGATTTAATTTATTACTAGAGTTATTACAACTACCTGCTGCTCCAAAAAGTATTCCTA
TCAATACATTAATACCTGTTAAAGGAACTCCTTTAGGAGATAAGTATACAAATGCACAAATTGATAGTTTTGAATTAGTA
AGATTTATTGCAACTACAAGAATACTATTTCCACAAGCACGCCTAAGGTTATCAGCAGGTAGAGAAAATATGTCTTTGGA
AACTCAAACTCTATGTTTCCTTGCTGGTATTAACTCAATATTTTATGGTAATAAGTTATTAACAGAAAATAATGCTACTG
TTAATTCTGATAATTTTTTATTAGCTAAGCTTGGCTTAAAATCAAATGCTGAATTATGTTAA

Upstream 100 bases:

>100_bases
AGCTATACATATTGTAAACCTTTTGTAAAATATTAAGTTGACATAAATTGTACACAAGATATAAAATACTTTACAACTTT
AAAAAACTTTTTTATAAAAA

Downstream 100 bases:

>100_bases
ACCTACAAGATAAATATACTCAATATCAAAGAGATAATTTATTAAGAGAGTTAACTCCTTTTATAAAAGATGACTCTATT
ATAGATTTTACAACTAGTGA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTLQQIKEIYSRPLTELILQALEIHNKNFGNDIELCSLKSIKTGTCPEDCKYCPQSGHYNTSIEKHKLLDKDSILAEAKN
AKDAGSKRFCMGAAWKHIPKKDFDQVAEIITEVKNLGLETCVTLGSINADEATKLKQAGLDYYNHNLDTSREFYPEIITT
RKFEERIETIRNVANADINVCCGGILGMGESLDDRFNLLLELLQLPAAPKSIPINTLIPVKGTPLGDKYTNAQIDSFELV
RFIATTRILFPQARLRLSAGRENMSLETQTLCFLAGINSIFYGNKLLTENNATVNSDNFLLAKLGLKSNAELC

Sequences:

>Translated_313_residues
MTLQQIKEIYSRPLTELILQALEIHNKNFGNDIELCSLKSIKTGTCPEDCKYCPQSGHYNTSIEKHKLLDKDSILAEAKN
AKDAGSKRFCMGAAWKHIPKKDFDQVAEIITEVKNLGLETCVTLGSINADEATKLKQAGLDYYNHNLDTSREFYPEIITT
RKFEERIETIRNVANADINVCCGGILGMGESLDDRFNLLLELLQLPAAPKSIPINTLIPVKGTPLGDKYTNAQIDSFELV
RFIATTRILFPQARLRLSAGRENMSLETQTLCFLAGINSIFYGNKLLTENNATVNSDNFLLAKLGLKSNAELC
>Mature_312_residues
TLQQIKEIYSRPLTELILQALEIHNKNFGNDIELCSLKSIKTGTCPEDCKYCPQSGHYNTSIEKHKLLDKDSILAEAKNA
KDAGSKRFCMGAAWKHIPKKDFDQVAEIITEVKNLGLETCVTLGSINADEATKLKQAGLDYYNHNLDTSREFYPEIITTR
KFEERIETIRNVANADINVCCGGILGMGESLDDRFNLLLELLQLPAAPKSIPINTLIPVKGTPLGDKYTNAQIDSFELVR
FIATTRILFPQARLRLSAGRENMSLETQTLCFLAGINSIFYGNKLLTENNATVNSDNFLLAKLGLKSNAELC

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=306, Percent_Identity=50.6535947712418, Blast_Score=337, Evalue=6e-94,
Organism=Saccharomyces cerevisiae, GI6321725, Length=312, Percent_Identity=44.8717948717949, Blast_Score=286, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 34881; Mature: 34750

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQQIKEIYSRPLTELILQALEIHNKNFGNDIELCSLKSIKTGTCPEDCKYCPQSGHYN
CCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCCCCC
TSIEKHKLLDKDSILAEAKNAKDAGSKRFCMGAAWKHIPKKDFDQVAEIITEVKNLGLET
CCHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHH
CVTLGSINADEATKLKQAGLDYYNHNLDTSREFYPEIITTRKFEERIETIRNVANADINV
HHHHCCCCCHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
CCGGILGMGESLDDRFNLLLELLQLPAAPKSIPINTLIPVKGTPLGDKYTNAQIDSFELV
EHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHH
RFIATTRILFPQARLRLSAGRENMSLETQTLCFLAGINSIFYGNKLLTENNATVNSDNFL
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCEE
LAKLGLKSNAELC
EEEECCCCCCCCC
>Mature Secondary Structure 
TLQQIKEIYSRPLTELILQALEIHNKNFGNDIELCSLKSIKTGTCPEDCKYCPQSGHYN
CHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCCCCC
TSIEKHKLLDKDSILAEAKNAKDAGSKRFCMGAAWKHIPKKDFDQVAEIITEVKNLGLET
CCHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHH
CVTLGSINADEATKLKQAGLDYYNHNLDTSREFYPEIITTRKFEERIETIRNVANADINV
HHHHCCCCCHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
CCGGILGMGESLDDRFNLLLELLQLPAAPKSIPINTLIPVKGTPLGDKYTNAQIDSFELV
EHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHH
RFIATTRILFPQARLRLSAGRENMSLETQTLCFLAGINSIFYGNKLLTENNATVNSDNFL
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCEE
LAKLGLKSNAELC
EEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA