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The map label for this gene is lpdA [H]

Identifier: 134301834

GI number: 134301834

Start: 788856

End: 790268

Strand: Direct

Name: lpdA [H]

Synonym: FTW_0810

Alternate gene names: 134301834

Gene position: 788856-790268 (Clockwise)

Preceding gene: 134301833

Following gene: 134301835

Centisome position: 41.55

GC content: 39.21

Gene sequence:

>1413_bases
ATGAGTGATATTAAAACACAAGTTGTAGTTTTAGGTAGTGGTCCTGGTGGATATAGTGCGGCTTTTAGAGCAGCTGACTT
AGGATTAGAAGTTGTTTTAGTAGAGAGATATGCCGAGATCGGTGGTGTGTGCCTAAATGTGGGATGTATCCCATCAAAAG
CTATGTTGCATATTGCCAAAGTTATCAATGAGGCTCGTCATCTAGAATCTCTTGGTATAATCGAGATGGGTGGCCTAAAA
ATCAATAGAGAAAACCTCTTAAAATATAAAGATGGGGTTATCGGTAAGCTTACTGGTGGTCTAAAGGGTATGGCTCAGAT
GAGAAAAGTAAAAATCGTACAAGGTTACGGTAAATTTACTTCTGATAAAGAACTTGCTGTTGAGGCTGCTGATGGTAAAG
TTACAAAAATTGCTTTTGATAACTGTATTATTGCCGCGGGTTCTAGTGTTATTAAGCTACCTTTTGTACCAGAGGACGAT
AGAATTATTGACTCTACAGGCGCTCTTGAGATGAAAGAGATTCCAGAAACTATGCTCGTAGTTGGTGGTGGAATTATCGG
TCTTGAGATGGCACAAGTATATTCTGAGTTAGGTACGAAGATCACGGTGGTTGAGTTTGCTGATCAGCTTATGAATGGTG
TCGATAAGGATCTAGTCAAAGCTTATCAAAAAGTAAATAGTCGTTATGATGTGCGCCTAAAAACAGCTGTAACAGCTATG
GAAGCTAAAGAAGATGGTATCTATGTAACTATGGAAGGCGATCATCCTGCAAAAGATGAGAGATTCGATAGAGTACTTAT
GGCTATTGGTCGTAAACCAAATGGTAAACTAATTGATGCTGAAAAAGCAGGTGTTAAAGTTGATGAGAGAGGCTTTATCC
CAGTAGATAAGCAATTACGTACAAATGTGCCTCACATTTTTGCTATTGGAGATATTGTTGGTCAACCTATGCTTGCTCAT
AAAGCTGTACCGGAAGGTAGAACAGCTGCTGAAGTAATATCAGGACTAAATCATAGTTTTGATCCTTTAGTGATTCCTTC
AGTTGCTTATACTGATCCAGAGGTTGCTTGGGTTGGTGAGACTGAGACTTCTGCAAAAGCTAAAGGTATCAAGTATGAAA
AAGGTGTATTCCCATGGGCAGCTAGTGGTAGATCGCTAAGTATCGATAGATCAGAGGGTATGACAAAAATTCTATTTGAT
GAAAATCATAAGATTATTGGAGCTTCTATCGTTGGTACTCATGCTGGCGAGCTTATTTCAGAAGCAGCTCTTGCAATAGA
AATGGGTTGTGATGCTGAAGATATAGCTCTTACAGTACATCCACATCCAACTCTATCTGAAAGCTTAATGATGGCTACAG
AAGTTTATGAAGGTACTGCTACAGATCTTCCGCCGCAAAAGAAGAAAAAGTAA

Upstream 100 bases:

>100_bases
CATAGAGTGATAGATGGCGCATTAGCAGCTAAATTCTTAACTAGATATTGTCAGATTTTATCTGATTTACGTGAAATCAT
AATGTAAGCGGAGTTTTAAA

Downstream 100 bases:

>100_bases
GGGGCTGTCCTAGATAAGTTTACTTTAAAGTCTCTCTAACCCAGTGTCTAATTAGCTTTAGTTGCTCTTTAGAATCACTG
TGGTTAAATCTCCACTCACA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSDIKTQVVVLGSGPGGYSAAFRAADLGLEVVLVERYAEIGGVCLNVGCIPSKAMLHIAKVINEARHLESLGIIEMGGLK
INRENLLKYKDGVIGKLTGGLKGMAQMRKVKIVQGYGKFTSDKELAVEAADGKVTKIAFDNCIIAAGSSVIKLPFVPEDD
RIIDSTGALEMKEIPETMLVVGGGIIGLEMAQVYSELGTKITVVEFADQLMNGVDKDLVKAYQKVNSRYDVRLKTAVTAM
EAKEDGIYVTMEGDHPAKDERFDRVLMAIGRKPNGKLIDAEKAGVKVDERGFIPVDKQLRTNVPHIFAIGDIVGQPMLAH
KAVPEGRTAAEVISGLNHSFDPLVIPSVAYTDPEVAWVGETETSAKAKGIKYEKGVFPWAASGRSLSIDRSEGMTKILFD
ENHKIIGASIVGTHAGELISEAALAIEMGCDAEDIALTVHPHPTLSESLMMATEVYEGTATDLPPQKKKK

Sequences:

>Translated_470_residues
MSDIKTQVVVLGSGPGGYSAAFRAADLGLEVVLVERYAEIGGVCLNVGCIPSKAMLHIAKVINEARHLESLGIIEMGGLK
INRENLLKYKDGVIGKLTGGLKGMAQMRKVKIVQGYGKFTSDKELAVEAADGKVTKIAFDNCIIAAGSSVIKLPFVPEDD
RIIDSTGALEMKEIPETMLVVGGGIIGLEMAQVYSELGTKITVVEFADQLMNGVDKDLVKAYQKVNSRYDVRLKTAVTAM
EAKEDGIYVTMEGDHPAKDERFDRVLMAIGRKPNGKLIDAEKAGVKVDERGFIPVDKQLRTNVPHIFAIGDIVGQPMLAH
KAVPEGRTAAEVISGLNHSFDPLVIPSVAYTDPEVAWVGETETSAKAKGIKYEKGVFPWAASGRSLSIDRSEGMTKILFD
ENHKIIGASIVGTHAGELISEAALAIEMGCDAEDIALTVHPHPTLSESLMMATEVYEGTATDLPPQKKKK
>Mature_469_residues
SDIKTQVVVLGSGPGGYSAAFRAADLGLEVVLVERYAEIGGVCLNVGCIPSKAMLHIAKVINEARHLESLGIIEMGGLKI
NRENLLKYKDGVIGKLTGGLKGMAQMRKVKIVQGYGKFTSDKELAVEAADGKVTKIAFDNCIIAAGSSVIKLPFVPEDDR
IIDSTGALEMKEIPETMLVVGGGIIGLEMAQVYSELGTKITVVEFADQLMNGVDKDLVKAYQKVNSRYDVRLKTAVTAME
AKEDGIYVTMEGDHPAKDERFDRVLMAIGRKPNGKLIDAEKAGVKVDERGFIPVDKQLRTNVPHIFAIGDIVGQPMLAHK
AVPEGRTAAEVISGLNHSFDPLVIPSVAYTDPEVAWVGETETSAKAKGIKYEKGVFPWAASGRSLSIDRSEGMTKILFDE
NHKIIGASIVGTHAGELISEAALAIEMGCDAEDIALTVHPHPTLSESLMMATEVYEGTATDLPPQKKKK

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=460, Percent_Identity=41.7391304347826, Blast_Score=341, Evalue=9e-94,
Organism=Homo sapiens, GI50301238, Length=455, Percent_Identity=28.5714285714286, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI291045266, Length=447, Percent_Identity=26.3982102908277, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI33519430, Length=426, Percent_Identity=26.056338028169, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI33519428, Length=426, Percent_Identity=26.056338028169, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI33519426, Length=426, Percent_Identity=26.056338028169, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI148277065, Length=426, Percent_Identity=26.056338028169, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI148277071, Length=426, Percent_Identity=26.056338028169, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI22035672, Length=463, Percent_Identity=28.0777537796976, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI291045268, Length=441, Percent_Identity=25.1700680272109, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI21389617, Length=148, Percent_Identity=31.7567567567568, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI226437568, Length=148, Percent_Identity=31.7567567567568, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI65787454, Length=148, Percent_Identity=31.7567567567568, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=64.9789029535865, Blast_Score=599, Evalue=1e-173,
Organism=Escherichia coli, GI87082354, Length=456, Percent_Identity=30.2631578947368, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI87081717, Length=453, Percent_Identity=27.1523178807947, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=29.7482837528604, Blast_Score=154, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32565766, Length=464, Percent_Identity=39.0086206896552, Blast_Score=325, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=28.2105263157895, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=443, Percent_Identity=26.86230248307, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=443, Percent_Identity=26.86230248307, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=439, Percent_Identity=25.0569476082005, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=192, Percent_Identity=28.6458333333333, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=462, Percent_Identity=41.7748917748918, Blast_Score=319, Evalue=6e-88,
Organism=Saccharomyces cerevisiae, GI6325240, Length=480, Percent_Identity=30.4166666666667, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6325166, Length=455, Percent_Identity=27.6923076923077, Blast_Score=157, Evalue=5e-39,
Organism=Drosophila melanogaster, GI21358499, Length=455, Percent_Identity=40.6593406593407, Blast_Score=334, Evalue=8e-92,
Organism=Drosophila melanogaster, GI17737741, Length=473, Percent_Identity=27.061310782241, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24640553, Length=467, Percent_Identity=29.3361884368308, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24640549, Length=467, Percent_Identity=29.3361884368308, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24640551, Length=467, Percent_Identity=29.3361884368308, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50486; Mature: 50355

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIKTQVVVLGSGPGGYSAAFRAADLGLEVVLVERYAEIGGVCLNVGCIPSKAMLHIAK
CCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEHHHHHHCCEEEEECCCCHHHHHHHHH
VINEARHLESLGIIEMGGLKINRENLLKYKDGVIGKLTGGLKGMAQMRKVKIVQGYGKFT
HHHHHHHHHHCCEEEECCEEECHHHHHHHCCCCEEECCCCHHHHHHHHHEEEEECCCCCC
SDKELAVEAADGKVTKIAFDNCIIAAGSSVIKLPFVPEDDRIIDSTGALEMKEIPETMLV
CCCEEEEEECCCCEEEEEECCEEEECCCCEEEECCCCCCCCEECCCCCEEHHHCCCEEEE
VGGGIIGLEMAQVYSELGTKITVVEFADQLMNGVDKDLVKAYQKVNSRYDVRLKTAVTAM
ECCCCHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEHHHHHH
EAKEDGIYVTMEGDHPAKDERFDRVLMAIGRKPNGKLIDAEKAGVKVDERGFIPVDKQLR
CCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCEECCCCCCCCCHHHH
TNVPHIFAIGDIVGQPMLAHKAVPEGRTAAEVISGLNHSFDPLVIPSVAYTDPEVAWVGE
CCCCEEEEEHHHHCCCHHHHHCCCCCCHHHHHHHHCCCCCCCEEECCEECCCCCEEEECC
TETSAKAKGIKYEKGVFPWAASGRSLSIDRSEGMTKILFDENHKIIGASIVGTHAGELIS
CCCCHHHCCCEECCCCCCCCCCCCEEEEECCCCCEEEEECCCCEEEEEEEECCHHHHHHH
EAALAIEMGCDAEDIALTVHPHPTLSESLMMATEVYEGTATDLPPQKKKK
HHHHEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDIKTQVVVLGSGPGGYSAAFRAADLGLEVVLVERYAEIGGVCLNVGCIPSKAMLHIAK
CCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEHHHHHHCCEEEEECCCCHHHHHHHHH
VINEARHLESLGIIEMGGLKINRENLLKYKDGVIGKLTGGLKGMAQMRKVKIVQGYGKFT
HHHHHHHHHHCCEEEECCEEECHHHHHHHCCCCEEECCCCHHHHHHHHHEEEEECCCCCC
SDKELAVEAADGKVTKIAFDNCIIAAGSSVIKLPFVPEDDRIIDSTGALEMKEIPETMLV
CCCEEEEEECCCCEEEEEECCEEEECCCCEEEECCCCCCCCEECCCCCEEHHHCCCEEEE
VGGGIIGLEMAQVYSELGTKITVVEFADQLMNGVDKDLVKAYQKVNSRYDVRLKTAVTAM
ECCCCHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEHHHHHH
EAKEDGIYVTMEGDHPAKDERFDRVLMAIGRKPNGKLIDAEKAGVKVDERGFIPVDKQLR
CCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCEECCCCCCCCCHHHH
TNVPHIFAIGDIVGQPMLAHKAVPEGRTAAEVISGLNHSFDPLVIPSVAYTDPEVAWVGE
CCCCEEEEEHHHHCCCHHHHHCCCCCCHHHHHHHHCCCCCCCEEECCEECCCCCEEEECC
TETSAKAKGIKYEKGVFPWAASGRSLSIDRSEGMTKILFDENHKIIGASIVGTHAGELIS
CCCCHHHCCCEECCCCCCCCCCCCEEEEECCCCCEEEEECCCCEEEEEEEECCHHHHHHH
EAALAIEMGCDAEDIALTVHPHPTLSESLMMATEVYEGTATDLPPQKKKK
HHHHEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]