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The map label for this gene is ubiE [H]

Identifier: 134301783

GI number: 134301783

Start: 726279

End: 727031

Strand: Reverse

Name: ubiE [H]

Synonym: FTW_0751

Alternate gene names: 134301783

Gene position: 727031-726279 (Counterclockwise)

Preceding gene: 134301785

Following gene: 134301782

Centisome position: 38.3

GC content: 34.93

Gene sequence:

>753_bases
ATGTCTAAAGAAAATAAAACAACAGATTTTGGTTTTACACAAGTGCCTTGGGAAGAAAAACAAAAAAAGGTAGCTGGGGT
ATTCCATTCAGTAGCAGCTAAATATGACTTGATGAATGATCTAATGTCATTTGGTATTCATCGCATCTGGAAAAAACAAA
CCATTGCTAAATCTGGAGTTCATAAGGGTGATAATGTCTTAGATCTTGCTGGCGGCACAGGTGACTTAGCATATAAATTT
TGTCAAATGGTTGGTCAACAAGGTAAAGTAATCTTGAGTGATATTAACTCCTCGATGCTTGAAGTAGGCAAAGAAAAACT
TACAAACAAAGGTTGTGTTGGCAATATCGAATATGTCCAAGCTAACGCTGAGTGTCTACCTTTCCCTGATAATTATTTTG
ATTGTATTACGATATCATTTGGACTTAGAAATGTTACAGACAAAGATAAAGCGTTAGCATCAATGTGTCGAGTTCTAAAA
CCAGGTGGTCGCTTGTTAGTTCTAGAATTCTCTAAGCCAATCATACCTTTACTTTCTAAAGTTTATGATGAATACTCTTT
CAAAGCACTACCATTTTTAGGTAAAATTATCACACAAGATGCCGAAAGCTACAAATACCTTGCTGAATCAATCCGCAAAC
ATCCTGATCAACAAACACTAAAACAGATGATGTACGATGCTGGATTTGATAACGTCGAATATCAAAATATGACCGGTGGA
ATTGTAGCATTACATATTGGATATAAATATTAA

Upstream 100 bases:

>100_bases
TATACATATTTATACTCCCTATTTAATGGTATCTTGCCAATACAAATAATTGATATTCTGCTATAATGAAATAATATCTC
AATATTACAATTTAGTTTAG

Downstream 100 bases:

>100_bases
GATGCTAAAACTAGTAAACACAGCCTTAAGTCTTCTGCCTAAATTTGATCCTCAGGTAAGTGCTCTACTGTTACCCATAA
ATGGTAAAGCCCTAAGTGTC

Product: menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSKENKTTDFGFTQVPWEEKQKKVAGVFHSVAAKYDLMNDLMSFGIHRIWKKQTIAKSGVHKGDNVLDLAGGTGDLAYKF
CQMVGQQGKVILSDINSSMLEVGKEKLTNKGCVGNIEYVQANAECLPFPDNYFDCITISFGLRNVTDKDKALASMCRVLK
PGGRLLVLEFSKPIIPLLSKVYDEYSFKALPFLGKIITQDAESYKYLAESIRKHPDQQTLKQMMYDAGFDNVEYQNMTGG
IVALHIGYKY

Sequences:

>Translated_250_residues
MSKENKTTDFGFTQVPWEEKQKKVAGVFHSVAAKYDLMNDLMSFGIHRIWKKQTIAKSGVHKGDNVLDLAGGTGDLAYKF
CQMVGQQGKVILSDINSSMLEVGKEKLTNKGCVGNIEYVQANAECLPFPDNYFDCITISFGLRNVTDKDKALASMCRVLK
PGGRLLVLEFSKPIIPLLSKVYDEYSFKALPFLGKIITQDAESYKYLAESIRKHPDQQTLKQMMYDAGFDNVEYQNMTGG
IVALHIGYKY
>Mature_249_residues
SKENKTTDFGFTQVPWEEKQKKVAGVFHSVAAKYDLMNDLMSFGIHRIWKKQTIAKSGVHKGDNVLDLAGGTGDLAYKFC
QMVGQQGKVILSDINSSMLEVGKEKLTNKGCVGNIEYVQANAECLPFPDNYFDCITISFGLRNVTDKDKALASMCRVLKP
GGRLLVLEFSKPIIPLLSKVYDEYSFKALPFLGKIITQDAESYKYLAESIRKHPDQQTLKQMMYDAGFDNVEYQNMTGGI
VALHIGYKY

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=284, Percent_Identity=45.0704225352113, Blast_Score=239, Evalue=1e-63,
Organism=Escherichia coli, GI48994983, Length=250, Percent_Identity=69.6, Blast_Score=369, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17557039, Length=250, Percent_Identity=45.6, Blast_Score=222, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6323526, Length=261, Percent_Identity=40.9961685823755, Blast_Score=177, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24641702, Length=262, Percent_Identity=43.1297709923664, Blast_Score=228, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28039; Mature: 27908

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS01183 UBIE_1 ; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKENKTTDFGFTQVPWEEKQKKVAGVFHSVAAKYDLMNDLMSFGIHRIWKKQTIAKSGV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HKGDNVLDLAGGTGDLAYKFCQMVGQQGKVILSDINSSMLEVGKEKLTNKGCVGNIEYVQ
CCCCCEEEECCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
ANAECLPFPDNYFDCITISFGLRNVTDKDKALASMCRVLKPGGRLLVLEFSKPIIPLLSK
ECCCEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHH
VYDEYSFKALPFLGKIITQDAESYKYLAESIRKHPDQQTLKQMMYDAGFDNVEYQNMTGG
HHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEECCCCCC
IVALHIGYKY
EEEEEECCCC
>Mature Secondary Structure 
SKENKTTDFGFTQVPWEEKQKKVAGVFHSVAAKYDLMNDLMSFGIHRIWKKQTIAKSGV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HKGDNVLDLAGGTGDLAYKFCQMVGQQGKVILSDINSSMLEVGKEKLTNKGCVGNIEYVQ
CCCCCEEEECCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
ANAECLPFPDNYFDCITISFGLRNVTDKDKALASMCRVLKPGGRLLVLEFSKPIIPLLSK
ECCCEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHH
VYDEYSFKALPFLGKIITQDAESYKYLAESIRKHPDQQTLKQMMYDAGFDNVEYQNMTGG
HHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEECCCCCC
IVALHIGYKY
EEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA