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The map label for this gene is queA [H]

Identifier: 134301766

GI number: 134301766

Start: 710152

End: 711168

Strand: Direct

Name: queA [H]

Synonym: FTW_0731

Alternate gene names: 134301766

Gene position: 710152-711168 (Clockwise)

Preceding gene: 134301765

Following gene: 134301767

Centisome position: 37.41

GC content: 34.61

Gene sequence:

>1017_bases
ATGAAAACAGATGATTTTGATTATAAATTACCAGAAGAGTTGATTGCTAGCTATCCATTAGAAAATCGTGATGCTAGTAG
ATTACTAAAGTTAAATAAGCAAACAGGTGAAATAGCGGATTATAAATTTACTGATTTTATTGATTTTATAAACCCGGGAG
ATTTACTTGTTTTTAATAACAGTAAAGTAATGTTAGCGCGTTTGTATGGTAGTAAAACTACTGGAGCAAAACTTGAGTAC
CTTATAGAAAGGATCAAAAACCCAAAACTATTTGAAACACATATCAAAGCTAACCGTTCACCAGCTATTGGTAGTGAAAT
ATATGTTGAGGATACTTTAGCAAAAGTTTTAGATAAAGATGGTGGTATGTATCTACTTGAAATACAGGGCGACAAAGATA
TTTATCAGTTGATGGAAGAGTTCGGTCATATTCCATTGCCACCATATATGAAGCGTGATGATGAGGAATTTGATGCAGAG
CGTTATCAGACTGTGTATGCTCAAGATCTGGGTTCAGTGGCAGCACCAACAGCTGGATTGCATTTCTCAAAAGAGTTAAT
GCAACAGATAAAAGATAAGGGTGTTGATATTGCTTATATAACACTGCATGTTGGTTCTGGAACTTTTAAACCAGTTCAAG
TTGATGATGTTGAGAGTCATAAAATGCATGCTGAAGTAATATCCGTACCAGTAGAGGTTTGTCAGAAAATTCGCCAAACA
AAAGAAAATGGTGGGAGAGTCATAGCTATCGGTACAACTTCTGTACGTTCACTTGAAACAGCAGGACAAAATGGTCAGAT
TGAACCTTATCAAGGTGAGACAGATATTTTCTTATACCCAGGTAAGAAGTTCAATGTTGTAGATGCTATGATTACAAACT
TTCATCTACCGAAGTCTACACTTATAATGCTTGTAAGTGCATTTGCAGATAAAGAAAAAATCATAAAAGCATACGAACAT
GCCATAGCTGAAAGATATAGATTCTTTAGTTATGGTGATGCGATGTTTATTTTTTAA

Upstream 100 bases:

>100_bases
GAAATTCTCAATGATTATGAATCACAAAAGATTCTAAAAGAAATAAAAGCAAATTTTAAAGGCACTAAAAAATAGTAATT
TTTGTAGTATAAGGTTGTTT

Downstream 100 bases:

>100_bases
GAGGTTCCTTGATTACCAATTGTTACACTAGATGTGGTTATTGTTTGTTCGGAAGGAAATCGAGATTGTTTATTTGAAAT
ACTAAAAAAAAATTATTTAG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKTDDFDYKLPEELIASYPLENRDASRLLKLNKQTGEIADYKFTDFIDFINPGDLLVFNNSKVMLARLYGSKTTGAKLEY
LIERIKNPKLFETHIKANRSPAIGSEIYVEDTLAKVLDKDGGMYLLEIQGDKDIYQLMEEFGHIPLPPYMKRDDEEFDAE
RYQTVYAQDLGSVAAPTAGLHFSKELMQQIKDKGVDIAYITLHVGSGTFKPVQVDDVESHKMHAEVISVPVEVCQKIRQT
KENGGRVIAIGTTSVRSLETAGQNGQIEPYQGETDIFLYPGKKFNVVDAMITNFHLPKSTLIMLVSAFADKEKIIKAYEH
AIAERYRFFSYGDAMFIF

Sequences:

>Translated_338_residues
MKTDDFDYKLPEELIASYPLENRDASRLLKLNKQTGEIADYKFTDFIDFINPGDLLVFNNSKVMLARLYGSKTTGAKLEY
LIERIKNPKLFETHIKANRSPAIGSEIYVEDTLAKVLDKDGGMYLLEIQGDKDIYQLMEEFGHIPLPPYMKRDDEEFDAE
RYQTVYAQDLGSVAAPTAGLHFSKELMQQIKDKGVDIAYITLHVGSGTFKPVQVDDVESHKMHAEVISVPVEVCQKIRQT
KENGGRVIAIGTTSVRSLETAGQNGQIEPYQGETDIFLYPGKKFNVVDAMITNFHLPKSTLIMLVSAFADKEKIIKAYEH
AIAERYRFFSYGDAMFIF
>Mature_338_residues
MKTDDFDYKLPEELIASYPLENRDASRLLKLNKQTGEIADYKFTDFIDFINPGDLLVFNNSKVMLARLYGSKTTGAKLEY
LIERIKNPKLFETHIKANRSPAIGSEIYVEDTLAKVLDKDGGMYLLEIQGDKDIYQLMEEFGHIPLPPYMKRDDEEFDAE
RYQTVYAQDLGSVAAPTAGLHFSKELMQQIKDKGVDIAYITLHVGSGTFKPVQVDDVESHKMHAEVISVPVEVCQKIRQT
KENGGRVIAIGTTSVRSLETAGQNGQIEPYQGETDIFLYPGKKFNVVDAMITNFHLPKSTLIMLVSAFADKEKIIKAYEH
AIAERYRFFSYGDAMFIF

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=343, Percent_Identity=50.1457725947522, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38312; Mature: 38312

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTDDFDYKLPEELIASYPLENRDASRLLKLNKQTGEIADYKFTDFIDFINPGDLLVFNN
CCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCEEEECC
SKVMLARLYGSKTTGAKLEYLIERIKNPKLFETHIKANRSPAIGSEIYVEDTLAKVLDKD
CCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCEEEHHHHHHHHHCCC
GGMYLLEIQGDKDIYQLMEEFGHIPLPPYMKRDDEEFDAERYQTVYAQDLGSVAAPTAGL
CCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
HFSKELMQQIKDKGVDIAYITLHVGSGTFKPVQVDDVESHKMHAEVISVPVEVCQKIRQT
HHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KENGGRVIAIGTTSVRSLETAGQNGQIEPYQGETDIFLYPGKKFNVVDAMITNFHLPKST
HHCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCHHH
LIMLVSAFADKEKIIKAYEHAIAERYRFFSYGDAMFIF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MKTDDFDYKLPEELIASYPLENRDASRLLKLNKQTGEIADYKFTDFIDFINPGDLLVFNN
CCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCEEEECC
SKVMLARLYGSKTTGAKLEYLIERIKNPKLFETHIKANRSPAIGSEIYVEDTLAKVLDKD
CCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCEEEHHHHHHHHHCCC
GGMYLLEIQGDKDIYQLMEEFGHIPLPPYMKRDDEEFDAERYQTVYAQDLGSVAAPTAGL
CCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
HFSKELMQQIKDKGVDIAYITLHVGSGTFKPVQVDDVESHKMHAEVISVPVEVCQKIRQT
HHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KENGGRVIAIGTTSVRSLETAGQNGQIEPYQGETDIFLYPGKKFNVVDAMITNFHLPKST
HHCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCHHH
LIMLVSAFADKEKIIKAYEHAIAERYRFFSYGDAMFIF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA