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The map label for this gene is emrA [H]

Identifier: 134301727

GI number: 134301727

Start: 665333

End: 666355

Strand: Reverse

Name: emrA [H]

Synonym: FTW_0687

Alternate gene names: 134301727

Gene position: 666355-665333 (Counterclockwise)

Preceding gene: 134301728

Following gene: 134301726

Centisome position: 35.1

GC content: 33.24

Gene sequence:

>1023_bases
ATGTCAGAAGAAAAATTACAACAATCAACAATCACAAGAAATAAAAAAAGCTCGAGATTTTCGCCTAAGAAAATTATCAT
TGGCTCTAGCATTATTACAATTACAGCTTTAGGAATCTATGGTTATTATAAATATAACAAGATCTTCCCTAGTACTGATA
ATGCTTATGTTAATGCTGATATAATTAGTATTTCACCTAAAGTTGGTGGATATATAGAAAAAGTCTATATCCACAACAAC
CAATTTGTTCACAAAGACGATAAACTTGTTCAGATTGATCCTAAAGATTATCAACTAAAAGTAGCTCAAGCAAACTCAAA
AATTATCCAAACTAAAGGGCAATTAGCTATAGCTCAAGAACAAGTAAATGTAGCTAAGTCAAACCTAACTAAAGCACAGT
CATCTTTAGATACTGCAACAAGTATGGCAGATAGATATATTAAACTTTACAAAGATGATGCTGGCTCTTTACAAGATGCA
CAAAAATACATAAACCAAAAGATCCAAGCTCAGAAAGCAAAAGATGAAGCTTACTCATCACTTAAGCAAGCCTTATTACA
AGTTGAAATTGCCAAAGCACAAATTGGTGCAGCCAAACTAAGCTATGATAATGCCAATCTAAATCTAGGTTATACAACAT
TGATAGCTCCTGATGATGGCTATGTATCAAATTTAAAACTTTACAAAGGACAACTAGTATCACCTGGACAAGCATTATTT
GGTTTTATTGATAATAAAAAATGGTGGATAGACGCTAACTTTAAAGAAACAGATCTTGATAGAATCAAGCCAGGGCAAAA
AGTTGAAATTGAGCTAGATATGTATAGCCATAGTTACGCTGGCAAAGTTGATAGTATCAGTTATGCTACTGGCTCAGTAT
TCTCATTACTACCACCTGAAAATGCTACTGGAAACTGGGTAAAAGTCACTCAGCGCTTCCCAGTAAAAATAATCCTAAAA
AATGATCCTAAATACCCACTCAGAGTTGGAGCAAGTGCAACAGTAAAAGTAGATACATTATAG

Upstream 100 bases:

>100_bases
ACTTAGTCTAATCTTAGCTATAGTACCATTTTTATTAAAAGAGCCACCTACAGGTGCGCCTGTTGCAGTAATGCATTAAA
TTATTATAGGATAATTAAAA

Downstream 100 bases:

>100_bases
GTCACAACAATGATACGAAATAAAATACTTCCTTTAGCGTTAATATCAGCAACCTTAATAACATCTAGCTGTAGCTTCTT
TAGCCCTGAGTATGAAAGAC

Product: efflux pump protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MSEEKLQQSTITRNKKSSRFSPKKIIIGSSIITITALGIYGYYKYNKIFPSTDNAYVNADIISISPKVGGYIEKVYIHNN
QFVHKDDKLVQIDPKDYQLKVAQANSKIIQTKGQLAIAQEQVNVAKSNLTKAQSSLDTATSMADRYIKLYKDDAGSLQDA
QKYINQKIQAQKAKDEAYSSLKQALLQVEIAKAQIGAAKLSYDNANLNLGYTTLIAPDDGYVSNLKLYKGQLVSPGQALF
GFIDNKKWWIDANFKETDLDRIKPGQKVEIELDMYSHSYAGKVDSISYATGSVFSLLPPENATGNWVKVTQRFPVKIILK
NDPKYPLRVGASATVKVDTL

Sequences:

>Translated_340_residues
MSEEKLQQSTITRNKKSSRFSPKKIIIGSSIITITALGIYGYYKYNKIFPSTDNAYVNADIISISPKVGGYIEKVYIHNN
QFVHKDDKLVQIDPKDYQLKVAQANSKIIQTKGQLAIAQEQVNVAKSNLTKAQSSLDTATSMADRYIKLYKDDAGSLQDA
QKYINQKIQAQKAKDEAYSSLKQALLQVEIAKAQIGAAKLSYDNANLNLGYTTLIAPDDGYVSNLKLYKGQLVSPGQALF
GFIDNKKWWIDANFKETDLDRIKPGQKVEIELDMYSHSYAGKVDSISYATGSVFSLLPPENATGNWVKVTQRFPVKIILK
NDPKYPLRVGASATVKVDTL
>Mature_339_residues
SEEKLQQSTITRNKKSSRFSPKKIIIGSSIITITALGIYGYYKYNKIFPSTDNAYVNADIISISPKVGGYIEKVYIHNNQ
FVHKDDKLVQIDPKDYQLKVAQANSKIIQTKGQLAIAQEQVNVAKSNLTKAQSSLDTATSMADRYIKLYKDDAGSLQDAQ
KYINQKIQAQKAKDEAYSSLKQALLQVEIAKAQIGAAKLSYDNANLNLGYTTLIAPDDGYVSNLKLYKGQLVSPGQALFG
FIDNKKWWIDANFKETDLDRIKPGQKVEIELDMYSHSYAGKVDSISYATGSVFSLLPPENATGNWVKVTQRFPVKIILKN
DPKYPLRVGASATVKVDTL

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=331, Percent_Identity=35.3474320241692, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1790519, Length=313, Percent_Identity=33.5463258785943, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1788711, Length=345, Percent_Identity=31.304347826087, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1789637, Length=326, Percent_Identity=26.6871165644172, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI87081951, Length=326, Percent_Identity=27.6073619631902, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790024, Length=269, Percent_Identity=26.3940520446097, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790012, Length=240, Percent_Identity=23.75, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787013, Length=245, Percent_Identity=26.9387755102041, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37840; Mature: 37708

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEKLQQSTITRNKKSSRFSPKKIIIGSSIITITALGIYGYYKYNKIFPSTDNAYVNAD
CCHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEEEHHEEEEECEECCCCCCCEEEEE
IISISPKVGGYIEKVYIHNNQFVHKDDKLVQIDPKDYQLKVAQANSKIIQTKGQLAIAQE
EEEECCCCCCEEEEEEEECCEEEECCCCEEEECCCCEEEEEEECCCEEEEECCCEEEEHH
QVNVAKSNLTKAQSSLDTATSMADRYIKLYKDDAGSLQDAQKYINQKIQAQKAKDEAYSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LKQALLQVEIAKAQIGAAKLSYDNANLNLGYTTLIAPDDGYVSNLKLYKGQLVSPGQALF
HHHHHHHHHHHHHHCCEEEEEECCCCEEECEEEEEECCCCCEEEEEEECCCCCCCCHHEE
GFIDNKKWWIDANFKETDLDRIKPGQKVEIELDMYSHSYAGKVDSISYATGSVFSLLPPE
EEECCEEEEEECCCCCCCHHHCCCCCEEEEEEEEECCCCCCCCCCEEECCCCEEEECCCC
NATGNWVKVTQRFPVKIILKNDPKYPLRVGASATVKVDTL
CCCCCEEEEEECCCEEEEEECCCCCCEEECCEEEEEEECC
>Mature Secondary Structure 
SEEKLQQSTITRNKKSSRFSPKKIIIGSSIITITALGIYGYYKYNKIFPSTDNAYVNAD
CHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEEEHHEEEEECEECCCCCCCEEEEE
IISISPKVGGYIEKVYIHNNQFVHKDDKLVQIDPKDYQLKVAQANSKIIQTKGQLAIAQE
EEEECCCCCCEEEEEEEECCEEEECCCCEEEECCCCEEEEEEECCCEEEEECCCEEEEHH
QVNVAKSNLTKAQSSLDTATSMADRYIKLYKDDAGSLQDAQKYINQKIQAQKAKDEAYSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LKQALLQVEIAKAQIGAAKLSYDNANLNLGYTTLIAPDDGYVSNLKLYKGQLVSPGQALF
HHHHHHHHHHHHHHCCEEEEEECCCCEEECEEEEEECCCCCEEEEEEECCCCCCCCHHEE
GFIDNKKWWIDANFKETDLDRIKPGQKVEIELDMYSHSYAGKVDSISYATGSVFSLLPPE
EEECCEEEEEECCCCCCCHHHCCCCCEEEEEEEEECCCCCCCCCCEEECCCCEEEECCCC
NATGNWVKVTQRFPVKIILKNDPKYPLRVGASATVKVDTL
CCCCCEEEEEECCCEEEEEECCCCCCEEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]