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The map label for this gene is ousA [H]

Identifier: 134301661

GI number: 134301661

Start: 594529

End: 595785

Strand: Direct

Name: ousA [H]

Synonym: FTW_0601

Alternate gene names: 134301661

Gene position: 594529-595785 (Clockwise)

Preceding gene: 134301660

Following gene: 134301662

Centisome position: 31.32

GC content: 35.0

Gene sequence:

>1257_bases
ATGAAACGCTTTTCCAAAAGACATGTTTTTTTTGCTTCGGCAAGTACTATTGTTGAGTGGTATGATTTTATGCTTTTTGC
ATATTTAACTCCTGTGATAGCAAGTATATTTTTCCCCGATTTTAGTAAATATACGGCAATTCTTATGACTTTTGGGGTTT
TTGCTGCAGGATTTTTGATGGGACCTATAGGCAGTGTGGTTATGGGTAGTTTTGGTGATCGCTTTGGCCGTAAAAAGGCG
TTGGTGATATCTATTATTATGATGATACTACCAATGCTAGTGATAGCTATATTACCAACTTACAGTAGTATTGGAGTTTT
GGCACCAATTATATTGGTTGTAATGCGTTTATTACAGGGGTTTTCTATTGGGGGATCTTATGGTGGCGTGATGGTTTTTA
TGATTGAATCAACTAAACCAAATCGTCGTGGCTTTATTGCTAGTTTTGCTACTATGTCTTCTGGCACAGGAGTTTTCTTA
GCTTCACTAGTTGCAATGTTATTATTTGGTTTATTTAGCCGTGAGATTCTTGATTTATGGGGCTGGCGAATAGGCTATAT
TATGGGTTTAATATTAGCGTTAATGGCTTTGGGGATGCGTTTAATTATACCGGAAAGTTATTCTTTTGAGGAGTTGGAGG
CGCAAGGAGATATTTCTGTCAAACCAGTAAGAGAGATGTTTAAAAAACAAACTAAGCCACTACTTCTAGCAATTGCTTTA
TCGGCTTATGCTAATATTATGTATTATTTGGTACTTTCTTACTTAAGTAATCATTTTATTGAATTACATTATTCAGAGTT
TTTTTCTTTAGCAGTTGTTACAGTATTTAGTTTAATTTTTTCTTTTTCAGCGCCATTATGGGGTTTATTGAGTGACTATT
TAGGTAGAAAGCCTTTGATAAAGTTCTCGATTTGGATATATTTAATATTTGCATATCCAAGCTTTATGCTAATGAATATG
GGAGTAGTTTACTTAGTGCTAGCTATGATTATTTTAAGTGTTCCACTAATGGCTATATGGGGAGCTTATGGAGCAGCTGC
ACCTGAGTTATTTGATACTAAATATCGCTATAGTGGTAATGGACTAAGCTATAATATAGGCAACTCTTTCTTTGGTGGAA
CTATACCGTTTATAGCTACTTCACTTGTGGTTACATCAGGTAGTATGCTAGCTCCAGCATGGCTCTTGATAGTGGCTTCT
ATTATTATGATACCTGTACTTTACTATATGCCTGAGACTAGATTTGTTGATATTTAG

Upstream 100 bases:

>100_bases
TAAACAGATTATCATTAATTAAATGATCAATCATTTGCAAAGTTGTTTGTTTATATATAAAGTATAGTCTTTAGAATTAA
TTAATTATTTTTTTAAATAA

Downstream 100 bases:

>100_bases
ATAGATTAAATTCTAATGCCTTTGTATATTATATACACAGTGATATAATCTAGATTGTAATTTATTACATAAAAGGTGAC
TACTTTGGATTTTTGGTTAA

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MKRFSKRHVFFASASTIVEWYDFMLFAYLTPVIASIFFPDFSKYTAILMTFGVFAAGFLMGPIGSVVMGSFGDRFGRKKA
LVISIIMMILPMLVIAILPTYSSIGVLAPIILVVMRLLQGFSIGGSYGGVMVFMIESTKPNRRGFIASFATMSSGTGVFL
ASLVAMLLFGLFSREILDLWGWRIGYIMGLILALMALGMRLIIPESYSFEELEAQGDISVKPVREMFKKQTKPLLLAIAL
SAYANIMYYLVLSYLSNHFIELHYSEFFSLAVVTVFSLIFSFSAPLWGLLSDYLGRKPLIKFSIWIYLIFAYPSFMLMNM
GVVYLVLAMIILSVPLMAIWGAYGAAAPELFDTKYRYSGNGLSYNIGNSFFGGTIPFIATSLVVTSGSMLAPAWLLIVAS
IIMIPVLYYMPETRFVDI

Sequences:

>Translated_418_residues
MKRFSKRHVFFASASTIVEWYDFMLFAYLTPVIASIFFPDFSKYTAILMTFGVFAAGFLMGPIGSVVMGSFGDRFGRKKA
LVISIIMMILPMLVIAILPTYSSIGVLAPIILVVMRLLQGFSIGGSYGGVMVFMIESTKPNRRGFIASFATMSSGTGVFL
ASLVAMLLFGLFSREILDLWGWRIGYIMGLILALMALGMRLIIPESYSFEELEAQGDISVKPVREMFKKQTKPLLLAIAL
SAYANIMYYLVLSYLSNHFIELHYSEFFSLAVVTVFSLIFSFSAPLWGLLSDYLGRKPLIKFSIWIYLIFAYPSFMLMNM
GVVYLVLAMIILSVPLMAIWGAYGAAAPELFDTKYRYSGNGLSYNIGNSFFGGTIPFIATSLVVTSGSMLAPAWLLIVAS
IIMIPVLYYMPETRFVDI
>Mature_418_residues
MKRFSKRHVFFASASTIVEWYDFMLFAYLTPVIASIFFPDFSKYTAILMTFGVFAAGFLMGPIGSVVMGSFGDRFGRKKA
LVISIIMMILPMLVIAILPTYSSIGVLAPIILVVMRLLQGFSIGGSYGGVMVFMIESTKPNRRGFIASFATMSSGTGVFL
ASLVAMLLFGLFSREILDLWGWRIGYIMGLILALMALGMRLIIPESYSFEELEAQGDISVKPVREMFKKQTKPLLLAIAL
SAYANIMYYLVLSYLSNHFIELHYSEFFSLAVVTVFSLIFSFSAPLWGLLSDYLGRKPLIKFSIWIYLIFAYPSFMLMNM
GVVYLVLAMIILSVPLMAIWGAYGAAAPELFDTKYRYSGNGLSYNIGNSFFGGTIPFIATSLVVTSGSMLAPAWLLIVAS
IIMIPVLYYMPETRFVDI

Specific function: Involved in uptake and accumulation of various osmoprotectants. Allows the uptake of glycine betaine, proline, ectoine, and pipecolic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=420, Percent_Identity=30.4761904761905, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788292, Length=388, Percent_Identity=29.639175257732, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1788942, Length=376, Percent_Identity=32.7127659574468, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789941, Length=422, Percent_Identity=30.3317535545024, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI87082231, Length=389, Percent_Identity=24.6786632390745, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46521; Mature: 46521

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00334 MYB_2 ; PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFSKRHVFFASASTIVEWYDFMLFAYLTPVIASIFFPDFSKYTAILMTFGVFAAGFLM
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GPIGSVVMGSFGDRFGRKKALVISIIMMILPMLVIAILPTYSSIGVLAPIILVVMRLLQG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
FSIGGSYGGVMVFMIESTKPNRRGFIASFATMSSGTGVFLASLVAMLLFGLFSREILDLW
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GWRIGYIMGLILALMALGMRLIIPESYSFEELEAQGDISVKPVREMFKKQTKPLLLAIAL
HHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHH
SAYANIMYYLVLSYLSNHFIELHYSEFFSLAVVTVFSLIFSFSAPLWGLLSDYLGRKPLI
HHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHH
KFSIWIYLIFAYPSFMLMNMGVVYLVLAMIILSVPLMAIWGAYGAAAPELFDTKYRYSGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEECCC
GLSYNIGNSFFGGTIPFIATSLVVTSGSMLAPAWLLIVASIIMIPVLYYMPETRFVDI
CEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
>Mature Secondary Structure
MKRFSKRHVFFASASTIVEWYDFMLFAYLTPVIASIFFPDFSKYTAILMTFGVFAAGFLM
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GPIGSVVMGSFGDRFGRKKALVISIIMMILPMLVIAILPTYSSIGVLAPIILVVMRLLQG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
FSIGGSYGGVMVFMIESTKPNRRGFIASFATMSSGTGVFLASLVAMLLFGLFSREILDLW
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GWRIGYIMGLILALMALGMRLIIPESYSFEELEAQGDISVKPVREMFKKQTKPLLLAIAL
HHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHH
SAYANIMYYLVLSYLSNHFIELHYSEFFSLAVVTVFSLIFSFSAPLWGLLSDYLGRKPLI
HHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHH
KFSIWIYLIFAYPSFMLMNMGVVYLVLAMIILSVPLMAIWGAYGAAAPELFDTKYRYSGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEECCC
GLSYNIGNSFFGGTIPFIATSLVVTSGSMLAPAWLLIVASIIMIPVLYYMPETRFVDI
CEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8550465 [H]