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The map label for this gene is gloB

Identifier: 134301554

GI number: 134301554

Start: 469372

End: 470130

Strand: Direct

Name: gloB

Synonym: FTW_0483

Alternate gene names: 134301554

Gene position: 469372-470130 (Clockwise)

Preceding gene: 134301553

Following gene: 134301556

Centisome position: 24.72

GC content: 30.7

Gene sequence:

>759_bases
ATGCAAATTAAAAGATGGTTTTTGAATAATAGCTTAAGAAACTATCAGTACCTCTTATATGATAAAAGCCATGCTATCGT
TATAGATCCTCTAAAATCTGATATTTTCGCTGAATTTATTGCAAAAAATAAATTACAGCTTGAGGCTATCTTAATTACAC
ATAAACATGGTGATCATATTGCAGGAGTCAAAAAATTATTAGCGATATATCTAAATGCTAAAGTTTATGCATATACTGGA
AATGATTTATTTAAGCCAGATATTTATGTTAAGGATGGAAGTTTTATAAATCTTGGATTTACTAGCTTTAGGGTTATGTA
TATTCCTGGTCATATAGATGATCATGTGTGTTTTTTATTTGAGCAAGAGCGAGCTCTTTTCTGTGGTGATACATTATTTA
ACGCGGGAGTAGGTGGTGTTCAAGCAGAATCAGCTGATATAAATCAATTATATGACTCATTAGTCAAAATTACTAAGTTG
GATGGTGATATTAAGCCATATCCTGCCCATGATTATTGGCTTGGCAATCTCGATTTTGCATTAAGTATTCTTGCTGATGA
TAGCTATTTTAACTATTATAGAAATCAAGTTGCCGAATTAGCAGCTGAAGATAAACCAATAGTCAATTTAGCAGAAGAAG
CCAAATTAAATATTTTTATCAGAGCGATGTCAGATAAAGCACTTTTGAAAGCATTGCCTGATTATAGTTTGGGTCGAGAA
ATGTTTGTTAAACTAAGACAATTAAAAAATAATTTTTAA

Upstream 100 bases:

>100_bases
CATATTTTATGTAAGTTTTTTATAAAAAATGGCGAAGTAGCAGGTATAGACTTATCAGTAGCAAGATTGGTTTATACCAA
TAACTAAAGGGGTTTTTCGT

Downstream 100 bases:

>100_bases
AACAGATGATTTTTCTTTAGCTTCTGCTAATTTGCTAAGTTAGTTTATTCCAAATTATCTAAATTGTATTTTATATATTA
ACTTTTTGAATTATTGTTGG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MQIKRWFLNNSLRNYQYLLYDKSHAIVIDPLKSDIFAEFIAKNKLQLEAILITHKHGDHIAGVKKLLAIYLNAKVYAYTG
NDLFKPDIYVKDGSFINLGFTSFRVMYIPGHIDDHVCFLFEQERALFCGDTLFNAGVGGVQAESADINQLYDSLVKITKL
DGDIKPYPAHDYWLGNLDFALSILADDSYFNYYRNQVAELAAEDKPIVNLAEEAKLNIFIRAMSDKALLKALPDYSLGRE
MFVKLRQLKNNF

Sequences:

>Translated_252_residues
MQIKRWFLNNSLRNYQYLLYDKSHAIVIDPLKSDIFAEFIAKNKLQLEAILITHKHGDHIAGVKKLLAIYLNAKVYAYTG
NDLFKPDIYVKDGSFINLGFTSFRVMYIPGHIDDHVCFLFEQERALFCGDTLFNAGVGGVQAESADINQLYDSLVKITKL
DGDIKPYPAHDYWLGNLDFALSILADDSYFNYYRNQVAELAAEDKPIVNLAEEAKLNIFIRAMSDKALLKALPDYSLGRE
MFVKLRQLKNNF
>Mature_252_residues
MQIKRWFLNNSLRNYQYLLYDKSHAIVIDPLKSDIFAEFIAKNKLQLEAILITHKHGDHIAGVKKLLAIYLNAKVYAYTG
NDLFKPDIYVKDGSFINLGFTSFRVMYIPGHIDDHVCFLFEQERALFCGDTLFNAGVGGVQAESADINQLYDSLVKITKL
DGDIKPYPAHDYWLGNLDFALSILADDSYFNYYRNQVAELAAEDKPIVNLAEEAKLNIFIRAMSDKALLKALPDYSLGRE
MFVKLRQLKNNF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538322, Length=219, Percent_Identity=28.310502283105, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI94538320, Length=219, Percent_Identity=28.310502283105, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI21703352, Length=263, Percent_Identity=27.7566539923954, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI116642887, Length=256, Percent_Identity=27.734375, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI14150041, Length=221, Percent_Identity=27.6018099547511, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI46361987, Length=177, Percent_Identity=29.3785310734463, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI41327741, Length=185, Percent_Identity=29.1891891891892, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI7705793, Length=170, Percent_Identity=28.2352941176471, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1786406, Length=246, Percent_Identity=34.9593495934959, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787158, Length=168, Percent_Identity=27.9761904761905, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17536925, Length=258, Percent_Identity=26.3565891472868, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320478, Length=280, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324614, Length=279, Percent_Identity=27.9569892473118, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356335, Length=254, Percent_Identity=26.7716535433071, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24667703, Length=254, Percent_Identity=26.7716535433071, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24667711, Length=254, Percent_Identity=26.7716535433071, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI221330176, Length=173, Percent_Identity=27.7456647398844, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_FRAT1 (Q14GJ6)

Other databases:

- EMBL:   AM286280
- RefSeq:   YP_667481.1
- ProteinModelPortal:   Q14GJ6
- SMR:   Q14GJ6
- STRING:   Q14GJ6
- GeneID:   4199792
- GenomeReviews:   AM286280_GR
- KEGG:   ftf:FTF1405c
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   NTHEAVW
- PhylomeDB:   Q14GJ6
- ProtClustDB:   CLSK935013
- BioCyc:   FTUL393115:FTF1405C-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28850; Mature: 28850

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKRWFLNNSLRNYQYLLYDKSHAIVIDPLKSDIFAEFIAKNKLQLEAILITHKHGDHI
CCCCHHHHCCCCCCEEEEEEECCCEEEEECCHHHHHHHHHHCCCEEEEEEEEEECCCCHH
AGVKKLLAIYLNAKVYAYTGNDLFKPDIYVKDGSFINLGFTSFRVMYIPGHIDDHVCFLF
HHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCEEEECCEEEEEEEECCCCCCEEEEEE
EQERALFCGDTLFNAGVGGVQAESADINQLYDSLVKITKLDGDIKPYPAHDYWLGNLDFA
ECCCEEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHEE
LSILADDSYFNYYRNQVAELAAEDKPIVNLAEEAKLNIFIRAMSDKALLKALPDYSLGRE
EEEEECCHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEEEECCHHHHHHCCCCCCCHH
MFVKLRQLKNNF
HHHHHHHHHCCC
>Mature Secondary Structure
MQIKRWFLNNSLRNYQYLLYDKSHAIVIDPLKSDIFAEFIAKNKLQLEAILITHKHGDHI
CCCCHHHHCCCCCCEEEEEEECCCEEEEECCHHHHHHHHHHCCCEEEEEEEEEECCCCHH
AGVKKLLAIYLNAKVYAYTGNDLFKPDIYVKDGSFINLGFTSFRVMYIPGHIDDHVCFLF
HHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCEEEECCEEEEEEEECCCCCCEEEEEE
EQERALFCGDTLFNAGVGGVQAESADINQLYDSLVKITKLDGDIKPYPAHDYWLGNLDFA
ECCCEEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHEE
LSILADDSYFNYYRNQVAELAAEDKPIVNLAEEAKLNIFIRAMSDKALLKALPDYSLGRE
EEEEECCHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEEEECCHHHHHHCCCCCCCHH
MFVKLRQLKNNF
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA