Click here to switch to the map view.

The map label for this gene is ppnK [H]

Identifier: 134301512

GI number: 134301512

Start: 429297

End: 430187

Strand: Reverse

Name: ppnK [H]

Synonym: FTW_0434

Alternate gene names: 134301512

Gene position: 430187-429297 (Counterclockwise)

Preceding gene: 134301514

Following gene: 134301510

Centisome position: 22.66

GC content: 34.23

Gene sequence:

>891_bases
ATGGCTTTTAAATATCATAAGGTTGCGATTGTTGGTAAGCATTATAAAAAAGAAGTAAGTCAAATGGTTGAAACTTTATA
TGCTTATTTACAGCAACAAGGCTTAGAAATAATTATAGAAAATGATACAGCAGCAGATACTTCACTTGTAAATGTTGCTA
TTGCTAGTCTAAAAGAGATTGCATTAAGATGTGATGTTGCGATAGTGGTTGGAGGTGATGGTAATTTTCTTAAGGCATCT
AGACTTTTGGCTTTGTACAGTAATATCCCAGTTATTGGTATAAACAAAGGCAAACTAGGATTTCTGACAACTCTTGCTGC
AGATGATAATGCTCTAAAGAATGATCTTTATGCGATACTGAAAGGTGATAGTTCAGTAACAAAAATGAGTATGCTAAAGT
ATCGTGTTGATAATAATTTGCGTGCACCATTAGAAGCCTCAATTGCCTTAAATGAGATAGCTATAACAGCTAGTAGAGGT
TTGATGTTTGGTTTGAAAGTTTTTATTGATGGTAGGTACGCTTTTGACCAAAGAGGTGATGGGCTTATTGTTTCTACACC
TACTGGTTCAACAGCACATGCGATGTCAGCGGGGGGACCAATTTTAAATCCTAATCAAAATAGTGTAGTTTTGGTACCAA
TATGTTCACACTCATTAAACAGTAGACCTTTAGTTATCTCAGATGAGAGTGTTATTGATATTTATATAACTGATTATAAT
GATCCTGAATCAGTCTTAAGTATTGATGGTAGACATGATACTATCCTCAAAGCACATCAGAAAGTAACTATCCAAAAAGC
GCGAAAGAAAGTTACAGTATTACATACAAAAGATTATAACTATTATGATACGCTAAGGGAGAAGTTGGGATGGAGTAAAG
TTCTGTTTTAG

Upstream 100 bases:

>100_bases
CTAAAATTTATTTTTGTAGTTAATATAACTCAAACGATAGTTATGCTACTAAGTCTTTTTGCTATAATACATCCAAGTTT
ATTTTTAAATTATATAAAAG

Downstream 100 bases:

>100_bases
AAAAGCTCAACTAATATAACTATCACAAAAATAGTGATAAAAATAGCTGAAATAAACTCTATCTTTTTAATATTTCTAAA
AAATACTTGTCTAAGTTTAT

Product: NAD(+)/NADH kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MAFKYHKVAIVGKHYKKEVSQMVETLYAYLQQQGLEIIIENDTAADTSLVNVAIASLKEIALRCDVAIVVGGDGNFLKAS
RLLALYSNIPVIGINKGKLGFLTTLAADDNALKNDLYAILKGDSSVTKMSMLKYRVDNNLRAPLEASIALNEIAITASRG
LMFGLKVFIDGRYAFDQRGDGLIVSTPTGSTAHAMSAGGPILNPNQNSVVLVPICSHSLNSRPLVISDESVIDIYITDYN
DPESVLSIDGRHDTILKAHQKVTIQKARKKVTVLHTKDYNYYDTLREKLGWSKVLF

Sequences:

>Translated_296_residues
MAFKYHKVAIVGKHYKKEVSQMVETLYAYLQQQGLEIIIENDTAADTSLVNVAIASLKEIALRCDVAIVVGGDGNFLKAS
RLLALYSNIPVIGINKGKLGFLTTLAADDNALKNDLYAILKGDSSVTKMSMLKYRVDNNLRAPLEASIALNEIAITASRG
LMFGLKVFIDGRYAFDQRGDGLIVSTPTGSTAHAMSAGGPILNPNQNSVVLVPICSHSLNSRPLVISDESVIDIYITDYN
DPESVLSIDGRHDTILKAHQKVTIQKARKKVTVLHTKDYNYYDTLREKLGWSKVLF
>Mature_295_residues
AFKYHKVAIVGKHYKKEVSQMVETLYAYLQQQGLEIIIENDTAADTSLVNVAIASLKEIALRCDVAIVVGGDGNFLKASR
LLALYSNIPVIGINKGKLGFLTTLAADDNALKNDLYAILKGDSSVTKMSMLKYRVDNNLRAPLEASIALNEIAITASRGL
MFGLKVFIDGRYAFDQRGDGLIVSTPTGSTAHAMSAGGPILNPNQNSVVLVPICSHSLNSRPLVISDESVIDIYITDYND
PESVLSIDGRHDTILKAHQKVTIQKARKKVTVLHTKDYNYYDTLREKLGWSKVLF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Homo sapiens, GI55743112, Length=305, Percent_Identity=27.8688524590164, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1788968, Length=298, Percent_Identity=40.2684563758389, Blast_Score=201, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6320794, Length=243, Percent_Identity=26.3374485596708, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322509, Length=257, Percent_Identity=25.2918287937743, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6325068, Length=239, Percent_Identity=28.0334728033473, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI28573828, Length=297, Percent_Identity=28.956228956229, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28573826, Length=297, Percent_Identity=28.956228956229, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI161077047, Length=297, Percent_Identity=28.956228956229, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28573830, Length=297, Percent_Identity=28.956228956229, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28573832, Length=297, Percent_Identity=28.956228956229, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24653424, Length=277, Percent_Identity=29.2418772563177, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281363323, Length=277, Percent_Identity=29.2418772563177, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281363321, Length=277, Percent_Identity=29.2418772563177, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20129957, Length=277, Percent_Identity=29.2418772563177, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24653422, Length=277, Percent_Identity=29.2418772563177, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 32476; Mature: 32345

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFKYHKVAIVGKHYKKEVSQMVETLYAYLQQQGLEIIIENDTAADTSLVNVAIASLKEI
CCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
ALRCDVAIVVGGDGNFLKASRLLALYSNIPVIGINKGKLGFLTTLAADDNALKNDLYAIL
HEEEEEEEEECCCCCEEHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCHHHCCEEEEE
KGDSSVTKMSMLKYRVDNNLRAPLEASIALNEIAITASRGLMFGLKVFIDGRYAFDQRGD
ECCCCHHHHHHHHEECCCCCCCCCCCEEEEEEEEEEECCCCEEEEEEEEECEEEECCCCC
GLIVSTPTGSTAHAMSAGGPILNPNQNSVVLVPICSHSLNSRPLVISDESVIDIYITDYN
EEEEECCCCCCCEECCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCEEEEEEECCC
DPESVLSIDGRHDTILKAHQKVTIQKARKKVTVLHTKDYNYYDTLREKLGWSKVLF
CCHHEEEECCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
AFKYHKVAIVGKHYKKEVSQMVETLYAYLQQQGLEIIIENDTAADTSLVNVAIASLKEI
CEEEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
ALRCDVAIVVGGDGNFLKASRLLALYSNIPVIGINKGKLGFLTTLAADDNALKNDLYAIL
HEEEEEEEEECCCCCEEHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCHHHCCEEEEE
KGDSSVTKMSMLKYRVDNNLRAPLEASIALNEIAITASRGLMFGLKVFIDGRYAFDQRGD
ECCCCHHHHHHHHEECCCCCCCCCCCEEEEEEEEEEECCCCEEEEEEEEECEEEECCCCC
GLIVSTPTGSTAHAMSAGGPILNPNQNSVVLVPICSHSLNSRPLVISDESVIDIYITDYN
EEEEECCCCCCCEECCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCEEEEEEECCC
DPESVLSIDGRHDTILKAHQKVTIQKARKKVTVLHTKDYNYYDTLREKLGWSKVLF
CCHHEEEECCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA