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The map label for this gene is wbtL [H]

Identifier: 134301505

GI number: 134301505

Start: 423136

End: 424020

Strand: Direct

Name: wbtL [H]

Synonym: FTW_0424

Alternate gene names: 134301505

Gene position: 423136-424020 (Clockwise)

Preceding gene: 134301504

Following gene: 134301506

Centisome position: 22.29

GC content: 38.53

Gene sequence:

>885_bases
ATGAAGGGAATAATTCTAGCTGGTGGCAGTGGTACAAGGCTATATCCACTTACCTTGGGTGTTAGCAAACAGCTGCTACC
TGTTTATGACAAGCCATTGTTATACTATCCACTATCTGTGCTTATGCTTGCAGGTATTAGGGAGATATTAATTATCTCTA
CAGTGCGTGATATCTCACTTATCCAAGAGCTTCTTGGTGATGGTTCACAATTTGGTATACAGTTGAGTTATAAAATCCAG
CCATCACCAGATGGGCTTGCTCAAGCATTTATTCTTGGTGAGGAGTTTTTGGCGGGTGACTCAGCTTGTTTGATATTAGG
AGATAATATCTACTATGGTCAAGGTATGACTACAATGCTAGAGTCTGCAAGAGCACAGTGTGGAGGTCCAGCTGGTGGCG
CTTGTGTTTTTGGTTATTATGTTAATGATCCGCATAGATATGGTATAGTCGAATTTGATAAGCAAAAAAATGTAATTTCG
GTAGAGGAAAAGCCACAGAATCCTAAGTCACACTATGCTATCACAGGTTTATATTTTTATGATAATAATGTTGTTGAGTA
TGCTAAACAAGTCAAACCATCTGCACGTGGTGAGCTAGAGATTACTTCACTTAATGAGTTATATCTAAAAGAAAATAAGC
TAAATGTCGAACTCTTAGGGCGTGGCTTTGCTTGGCTTGATGCTGGTACGCATGATTCATTGCTAGAGGCAGGTCAATAT
GTCGCAACTATTGAGAAAAGACAAGGGCTTAAAATTGCATGTTTGGAAGAAATTGCATGGCGTAAAGGCTTTATCTCAAC
ACAACAAGTTCTAGCTCAAGCTGAAAAACTTTCTAAGACAGAGTATGGTCAGTATCTGAAGAATTTAATTAAGGATAGTT
TATAA

Upstream 100 bases:

>100_bases
TCGTCTATTCAAAATTTTAATTAGATCAAGATGTATTCCATTAGTAAGTTGGTTTAAATATAGATTTGAAAAATAAATAT
CATTTAGAGGATTATGTGAA

Downstream 100 bases:

>100_bases
ATTAATCCGTCATACCCATGAAGGTGGGTATCTCATAAAAGTTGGATATGTTTTGGAGATTCCAATCTGCGCAGTAATGA
CAGGTTTGGTAATATATAGC

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 2; dTDP-glucose pyrophosphorylase 2; dTDP-glucose synthase 2 [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKGIILAGGSGTRLYPLTLGVSKQLLPVYDKPLLYYPLSVLMLAGIREILIISTVRDISLIQELLGDGSQFGIQLSYKIQ
PSPDGLAQAFILGEEFLAGDSACLILGDNIYYGQGMTTMLESARAQCGGPAGGACVFGYYVNDPHRYGIVEFDKQKNVIS
VEEKPQNPKSHYAITGLYFYDNNVVEYAKQVKPSARGELEITSLNELYLKENKLNVELLGRGFAWLDAGTHDSLLEAGQY
VATIEKRQGLKIACLEEIAWRKGFISTQQVLAQAEKLSKTEYGQYLKNLIKDSL

Sequences:

>Translated_294_residues
MKGIILAGGSGTRLYPLTLGVSKQLLPVYDKPLLYYPLSVLMLAGIREILIISTVRDISLIQELLGDGSQFGIQLSYKIQ
PSPDGLAQAFILGEEFLAGDSACLILGDNIYYGQGMTTMLESARAQCGGPAGGACVFGYYVNDPHRYGIVEFDKQKNVIS
VEEKPQNPKSHYAITGLYFYDNNVVEYAKQVKPSARGELEITSLNELYLKENKLNVELLGRGFAWLDAGTHDSLLEAGQY
VATIEKRQGLKIACLEEIAWRKGFISTQQVLAQAEKLSKTEYGQYLKNLIKDSL
>Mature_294_residues
MKGIILAGGSGTRLYPLTLGVSKQLLPVYDKPLLYYPLSVLMLAGIREILIISTVRDISLIQELLGDGSQFGIQLSYKIQ
PSPDGLAQAFILGEEFLAGDSACLILGDNIYYGQGMTTMLESARAQCGGPAGGACVFGYYVNDPHRYGIVEFDKQKNVIS
VEEKPQNPKSHYAITGLYFYDNNVVEYAKQVKPSARGELEITSLNELYLKENKLNVELLGRGFAWLDAGTHDSLLEAGQY
VATIEKRQGLKIACLEEIAWRKGFISTQQVLAQAEKLSKTEYGQYLKNLIKDSL

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=241, Percent_Identity=27.3858921161826, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI11761619, Length=241, Percent_Identity=27.3858921161826, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1790224, Length=291, Percent_Identity=67.0103092783505, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1788351, Length=292, Percent_Identity=64.3835616438356, Blast_Score=400, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI133931050, Length=240, Percent_Identity=27.9166666666667, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320148, Length=258, Percent_Identity=26.3565891472868, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355443, Length=253, Percent_Identity=26.4822134387352, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24644084, Length=253, Percent_Identity=26.4822134387352, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32433; Mature: 32433

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGSGTRLYPLTLGVSKQLLPVYDKPLLYYPLSVLMLAGIREILIISTVRDISL
CCCEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQELLGDGSQFGIQLSYKIQPSPDGLAQAFILGEEFLAGDSACLILGDNIYYGQGMTTML
HHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEECCCHHHHH
ESARAQCGGPAGGACVFGYYVNDPHRYGIVEFDKQKNVISVEEKPQNPKSHYAITGLYFY
HHHHHHCCCCCCCEEEEEEEECCCCEECCEEECCCCCEEEECCCCCCCCCCEEEEEEEEE
DNNVVEYAKQVKPSARGELEITSLNELYLKENKLNVELLGRGFAWLDAGTHDSLLEAGQY
CCCHHHHHHHCCCCCCCCEEEEECCEEEEECCCCEEEEEECCEEEEECCCCHHHHHCCHH
VATIEKRQGLKIACLEEIAWRKGFISTQQVLAQAEKLSKTEYGQYLKNLIKDSL
EEEHHHHCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGIILAGGSGTRLYPLTLGVSKQLLPVYDKPLLYYPLSVLMLAGIREILIISTVRDISL
CCCEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQELLGDGSQFGIQLSYKIQPSPDGLAQAFILGEEFLAGDSACLILGDNIYYGQGMTTML
HHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEECCCHHHHH
ESARAQCGGPAGGACVFGYYVNDPHRYGIVEFDKQKNVISVEEKPQNPKSHYAITGLYFY
HHHHHHCCCCCCCEEEEEEEECCCCEECCEEECCCCCEEEECCCCCCCCCCEEEEEEEEE
DNNVVEYAKQVKPSARGELEITSLNELYLKENKLNVELLGRGFAWLDAGTHDSLLEAGQY
CCCHHHHHHHCCCCCCCCEEEEECCEEEEECCCCEEEEEECCEEEEECCCCHHHHHCCHH
VATIEKRQGLKIACLEEIAWRKGFISTQQVLAQAEKLSKTEYGQYLKNLIKDSL
EEEHHHHCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]