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The map label for this gene is wbtA [H]

Identifier: 134301492

GI number: 134301492

Start: 408344

End: 409942

Strand: Direct

Name: wbtA [H]

Synonym: FTW_0411

Alternate gene names: 134301492

Gene position: 408344-409942 (Clockwise)

Preceding gene: 134301491

Following gene: 134301493

Centisome position: 21.51

GC content: 33.83

Gene sequence:

>1599_bases
TTGTCATCTTTTTTACTACTTAGAGATTATATGGCTAGTTGGCGTAAGTCGACTCAAAAAACTTTTTTACGTAAGGCTTT
TATTAATTTGCCAGTATTTTTCATAGTGGCATTATTTTTTTATGGCAAAGTCACTTTTTCGTTGATATTCTCTGAGTTTT
TATTTTATGTTTTTTTGATCAGTTTAAGTGTCTACTTTTATTGGTATTTGATGAACAGAGGATCAGTGGATAAAAGTAAA
ACTGCGGTTATTTATGGTGCAGGTGCTGCAGGAACAAAGATTGCTCAAGAACTTGCTTCTGCTGGTTATCGCATCAAATG
TTTTGTTGATGACAATGAAACTTTACAAAAAAGAAGTATTGATAGTAAAAAGGTTCTATCTAAAGCTGAATTAACAAAAC
TATTGCTATCTAGTAGATTTGACCTTTTGGTTATTGCATTGCCAAGAAATGCAAACCAAGTAGTCAAAAATATATATAAA
GAATTTGAAAAGGATTTTAATCAGATTAGAATTATGCCGCCTCTTGAGGAAATTCTTCAAGATGAGAATTTTATGTCACA
GTTGAAGCCTGTTTCACTCTATGATCTATTAGCGCGTGATACTAAGAGTTTAGATAAAGAATCTATCTCTAATTTTATCA
AAAATAAGGTGGTGCTAGTCACAGGAGCTGGAGGTAGTATAGGTTCTGAAATAGTACATCAATGTATCAAGTATCAGGCA
AAAGAGTTGATATTGGTTGATCATAGTGAGTTTAACTTATATAAAATTACTGAGGAGTGTAGTCATTTTAATATCAATAG
TGTGCTATGTTCTGTTTGTGATAGAAAAGCATTGGCTGAGGTTTTTCAAAAGTATACTCCAAATATAGTATTTCATGCTG
CTGCCTACAAGCATGTTCCCTTAGTTGAGGAGAATATCTCTAGAGCAATTAGAAATAATATCTTAGGTACTAAGAATGCT
ATAGATCTGGCTATAGAAGCTGGTGTTGAGTCATTTATATTGATTTCCACTGATAAAGCAGTGCGACCAACGAATGTTAT
GGGGGCTACCAAGAGAGTTTGTGAGCTGTATTTACAGAATGTTGATCCCAAAAATACCAAGCTTGCTGCAGTGCGTTTTG
GTAATGTGCTTGGTAGTAGTGGCAGTGTGATTCCAAAATTTGAAGAGCAAATAAGAAATGGTGGTCCTGTTACAGTTACT
CATCCTGAAATTACACGTTATTTTATGTTGATACCAGAAGCTTGTGAACTGGTCCTACAAGCTGGTGCTATTGCAAAAAA
TTCAGAGGTCTTTGTCTTAGATATGGGGCAACCTGTCAAGATTATTGATCTTGCTAAACAATTTATTAGACTTTCTGGTA
GAGGTGATATTGATATTAAAATAGTTGGTTTGCGTCCAGGAGAGAAACTTTACGAAGAGCTTTTGATAGAGGAAGATGAT
GTTAGTACCGACTATAAAGATATTTTTATTGGTAGAAGGACTTTTTACGATATTAATACTCTAAACCAAGATATTGAATC
GTTGATCAAGGATGATGTTGATCAGCTTGTGATATTAAAGAAAATTGTTCCGGAATTTGAACATAGATTGAATGGGTAG

Upstream 100 bases:

>100_bases
AATAATAGTTTTAACTATTATTACTGTTAATTGGACTTTCTATATTTTCAAGCAAGATGTTAATTTACATTTTTTACTTG
CATTAGTTTTGCTGAGATGC

Downstream 100 bases:

>100_bases
TGGTTTTATGTTTTATGAGGTTTTTAAAAGATTGCTTGATATTTTACTTTCTTTTATGGGGTTGTTGTTATTAAGTCCTA
TTTTCTTAATTATTATTTTT

Product: NAD dependent epimerase/dehydratase family protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MSSFLLLRDYMASWRKSTQKTFLRKAFINLPVFFIVALFFYGKVTFSLIFSEFLFYVFLISLSVYFYWYLMNRGSVDKSK
TAVIYGAGAAGTKIAQELASAGYRIKCFVDDNETLQKRSIDSKKVLSKAELTKLLLSSRFDLLVIALPRNANQVVKNIYK
EFEKDFNQIRIMPPLEEILQDENFMSQLKPVSLYDLLARDTKSLDKESISNFIKNKVVLVTGAGGSIGSEIVHQCIKYQA
KELILVDHSEFNLYKITEECSHFNINSVLCSVCDRKALAEVFQKYTPNIVFHAAAYKHVPLVEENISRAIRNNILGTKNA
IDLAIEAGVESFILISTDKAVRPTNVMGATKRVCELYLQNVDPKNTKLAAVRFGNVLGSSGSVIPKFEEQIRNGGPVTVT
HPEITRYFMLIPEACELVLQAGAIAKNSEVFVLDMGQPVKIIDLAKQFIRLSGRGDIDIKIVGLRPGEKLYEELLIEEDD
VSTDYKDIFIGRRTFYDINTLNQDIESLIKDDVDQLVILKKIVPEFEHRLNG

Sequences:

>Translated_532_residues
MSSFLLLRDYMASWRKSTQKTFLRKAFINLPVFFIVALFFYGKVTFSLIFSEFLFYVFLISLSVYFYWYLMNRGSVDKSK
TAVIYGAGAAGTKIAQELASAGYRIKCFVDDNETLQKRSIDSKKVLSKAELTKLLLSSRFDLLVIALPRNANQVVKNIYK
EFEKDFNQIRIMPPLEEILQDENFMSQLKPVSLYDLLARDTKSLDKESISNFIKNKVVLVTGAGGSIGSEIVHQCIKYQA
KELILVDHSEFNLYKITEECSHFNINSVLCSVCDRKALAEVFQKYTPNIVFHAAAYKHVPLVEENISRAIRNNILGTKNA
IDLAIEAGVESFILISTDKAVRPTNVMGATKRVCELYLQNVDPKNTKLAAVRFGNVLGSSGSVIPKFEEQIRNGGPVTVT
HPEITRYFMLIPEACELVLQAGAIAKNSEVFVLDMGQPVKIIDLAKQFIRLSGRGDIDIKIVGLRPGEKLYEELLIEEDD
VSTDYKDIFIGRRTFYDINTLNQDIESLIKDDVDQLVILKKIVPEFEHRLNG
>Mature_531_residues
SSFLLLRDYMASWRKSTQKTFLRKAFINLPVFFIVALFFYGKVTFSLIFSEFLFYVFLISLSVYFYWYLMNRGSVDKSKT
AVIYGAGAAGTKIAQELASAGYRIKCFVDDNETLQKRSIDSKKVLSKAELTKLLLSSRFDLLVIALPRNANQVVKNIYKE
FEKDFNQIRIMPPLEEILQDENFMSQLKPVSLYDLLARDTKSLDKESISNFIKNKVVLVTGAGGSIGSEIVHQCIKYQAK
ELILVDHSEFNLYKITEECSHFNINSVLCSVCDRKALAEVFQKYTPNIVFHAAAYKHVPLVEENISRAIRNNILGTKNAI
DLAIEAGVESFILISTDKAVRPTNVMGATKRVCELYLQNVDPKNTKLAAVRFGNVLGSSGSVIPKFEEQIRNGGPVTVTH
PEITRYFMLIPEACELVLQAGAIAKNSEVFVLDMGQPVKIIDLAKQFIRLSGRGDIDIKIVGLRPGEKLYEELLIEEDDV
STDYKDIFIGRRTFYDINTLNQDIESLIKDDVDQLVILKKIVPEFEHRLNG

Specific function: Involved in biofilm formation [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6319493, Length=206, Percent_Identity=31.0679611650485, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46

Molecular weight: Translated: 60225; Mature: 60093

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFLLLRDYMASWRKSTQKTFLRKAFINLPVFFIVALFFYGKVTFSLIFSEFLFYVFLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLSVYFYWYLMNRGSVDKSKTAVIYGAGAAGTKIAQELASAGYRIKCFVDDNETLQKRSI
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHCC
DSKKVLSKAELTKLLLSSRFDLLVIALPRNANQVVKNIYKEFEKDFNQIRIMPPLEEILQ
CHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHC
DENFMSQLKPVSLYDLLARDTKSLDKESISNFIKNKVVLVTGAGGSIGSEIVHQCIKYQA
CCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCC
KELILVDHSEFNLYKITEECSHFNINSVLCSVCDRKALAEVFQKYTPNIVFHAAAYKHVP
CEEEEEECCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCC
LVEENISRAIRNNILGTKNAIDLAIEAGVESFILISTDKAVRPTNVMGATKRVCELYLQN
CHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHC
VDPKNTKLAAVRFGNVLGSSGSVIPKFEEQIRNGGPVTVTHPEITRYFMLIPEACELVLQ
CCCCCCEEEEEEECHHHCCCCCCCCHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHH
AGAIAKNSEVFVLDMGQPVKIIDLAKQFIRLSGRGDIDIKIVGLRPGEKLYEELLIEEDD
HCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHCCCCCC
VSTDYKDIFIGRRTFYDINTLNQDIESLIKDDVDQLVILKKIVPEFEHRLNG
CCCCHHHHEECCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSFLLLRDYMASWRKSTQKTFLRKAFINLPVFFIVALFFYGKVTFSLIFSEFLFYVFLI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLSVYFYWYLMNRGSVDKSKTAVIYGAGAAGTKIAQELASAGYRIKCFVDDNETLQKRSI
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHCC
DSKKVLSKAELTKLLLSSRFDLLVIALPRNANQVVKNIYKEFEKDFNQIRIMPPLEEILQ
CHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHC
DENFMSQLKPVSLYDLLARDTKSLDKESISNFIKNKVVLVTGAGGSIGSEIVHQCIKYQA
CCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCC
KELILVDHSEFNLYKITEECSHFNINSVLCSVCDRKALAEVFQKYTPNIVFHAAAYKHVP
CEEEEEECCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCC
LVEENISRAIRNNILGTKNAIDLAIEAGVESFILISTDKAVRPTNVMGATKRVCELYLQN
CHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHC
VDPKNTKLAAVRFGNVLGSSGSVIPKFEEQIRNGGPVTVTHPEITRYFMLIPEACELVLQ
CCCCCCEEEEEEECHHHCCCCCCCCHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHH
AGAIAKNSEVFVLDMGQPVKIIDLAKQFIRLSGRGDIDIKIVGLRPGEKLYEELLIEEDD
HCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHCCCCCC
VSTDYKDIFIGRRTFYDINTLNQDIESLIKDDVDQLVILKKIVPEFEHRLNG
CCCCHHHHEECCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose

Specific reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]