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The map label for this gene is ispA [H]

Identifier: 134301456

GI number: 134301456

Start: 359353

End: 360234

Strand: Reverse

Name: ispA [H]

Synonym: FTW_0365

Alternate gene names: 134301456

Gene position: 360234-359353 (Counterclockwise)

Preceding gene: 134301457

Following gene: 134301455

Centisome position: 18.97

GC content: 30.16

Gene sequence:

>882_bases
ATGAGTAGTGAAAATATAATAAAAGATTTCGAAATTTTTGCTGAAAAGGTATTTGATCAACTTAACTGTGTATCCAAAAC
TTTACTAAGTGCAATGCACTATAGCTTTTTTAGTGGTGGCAAAAGAATAAGAGCACAATTTGTTTATGCTATTGGTGAAG
CTCTAGCTATAGATAAGTCAAACTGTGATAAAATCGCATTTGCAGTAGAGTCTATTCATACCTACTCACTAATACATGAT
GATCTTCCTGCTATGGATAACGATACTCTACGTAGGGGCAAACCAACTTGTCATATCAAATTTAATGAAGCTACTGCAAT
CTTAGCGGGCGATGCTTTACAAGCATTAGCTTTTGAAATATTACAAGATATCAACTACTCAGACATAGATAAACTAAAAA
AAATAAATAAACTTTTTGCTCAATACTGTGGTGCTAGTGGTATGGTTGGTGGTCAACAACTAGATATTGAGGGTGAGAAT
AAAAAACTTAAAATTGAAGAATTACAAATATTACATACCAATAAAACTGCAAAAATGTTTAGAGCATGTATAGTTTTACC
TTATATTCTCTCACAAAATCAAAATAGCCAAATAGAAGATTTATTAGTAAGGTTATCAGACTTAATAGGCTTATGTTTTC
AGATCAAAGATGATATCTTAGATGTCACTAAGACAACTAACGAGCTAGGAAAAACTAGCGCTAAAGATATAGATGCCAAT
AAATCAACCTATGTATCATTAATGGGATTAAAAGACGCAAATAAATATTTACTAGATAAAAAAAATGAAATCACACAAAT
TATCACTAAGCTAAAAAATAATAACTTCAGTTCAACTAGTCTTGAAAATCTCATTGATTTAGTTATTAATAGAAACTGTT
AG

Upstream 100 bases:

>100_bases
ATAAACAACTATTACAACAGATGTCATCGGGCGCTCTTAAGACTTTTAAAAGCCATAGTGATGTACTCGAAAAACAGTAT
AACAATATCGTAAAATTCTT

Downstream 100 bases:

>100_bases
TTATTTTTGCTACAATTCCGTAAATTTATTTAAAAGTTAATATTAAAATGCAAGTACAAACGATACAAAAAATATATGCT
TGGCTAGTGCATCTTTTTAC

Product: polyprenyl synthetase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSSENIIKDFEIFAEKVFDQLNCVSKTLLSAMHYSFFSGGKRIRAQFVYAIGEALAIDKSNCDKIAFAVESIHTYSLIHD
DLPAMDNDTLRRGKPTCHIKFNEATAILAGDALQALAFEILQDINYSDIDKLKKINKLFAQYCGASGMVGGQQLDIEGEN
KKLKIEELQILHTNKTAKMFRACIVLPYILSQNQNSQIEDLLVRLSDLIGLCFQIKDDILDVTKTTNELGKTSAKDIDAN
KSTYVSLMGLKDANKYLLDKKNEITQIITKLKNNNFSSTSLENLIDLVINRNC

Sequences:

>Translated_293_residues
MSSENIIKDFEIFAEKVFDQLNCVSKTLLSAMHYSFFSGGKRIRAQFVYAIGEALAIDKSNCDKIAFAVESIHTYSLIHD
DLPAMDNDTLRRGKPTCHIKFNEATAILAGDALQALAFEILQDINYSDIDKLKKINKLFAQYCGASGMVGGQQLDIEGEN
KKLKIEELQILHTNKTAKMFRACIVLPYILSQNQNSQIEDLLVRLSDLIGLCFQIKDDILDVTKTTNELGKTSAKDIDAN
KSTYVSLMGLKDANKYLLDKKNEITQIITKLKNNNFSSTSLENLIDLVINRNC
>Mature_292_residues
SSENIIKDFEIFAEKVFDQLNCVSKTLLSAMHYSFFSGGKRIRAQFVYAIGEALAIDKSNCDKIAFAVESIHTYSLIHDD
LPAMDNDTLRRGKPTCHIKFNEATAILAGDALQALAFEILQDINYSDIDKLKKINKLFAQYCGASGMVGGQQLDIEGENK
KLKIEELQILHTNKTAKMFRACIVLPYILSQNQNSQIEDLLVRLSDLIGLCFQIKDDILDVTKTTNELGKTSAKDIDANK
STYVSLMGLKDANKYLLDKKNEITQIITKLKNNNFSSTSLENLIDLVINRNC

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786623, Length=269, Percent_Identity=43.4944237918216, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1789578, Length=230, Percent_Identity=26.9565217391304, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 32848; Mature: 32717

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSENIIKDFEIFAEKVFDQLNCVSKTLLSAMHYSFFSGGKRIRAQFVYAIGEALAIDKS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
NCDKIAFAVESIHTYSLIHDDLPAMDNDTLRRGKPTCHIKFNEATAILAGDALQALAFEI
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCEEEEEECCCEEEEEHHHHHHHHHHH
LQDINYSDIDKLKKINKLFAQYCGASGMVGGQQLDIEGENKKLKIEELQILHTNKTAKMF
HHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEHHHEEEEECCHHHHHH
RACIVLPYILSQNQNSQIEDLLVRLSDLIGLCFQIKDDILDVTKTTNELGKTSAKDIDAN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KSTYVSLMGLKDANKYLLDKKNEITQIITKLKNNNFSSTSLENLIDLVINRNC
CCCEEEEEECCCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSENIIKDFEIFAEKVFDQLNCVSKTLLSAMHYSFFSGGKRIRAQFVYAIGEALAIDKS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
NCDKIAFAVESIHTYSLIHDDLPAMDNDTLRRGKPTCHIKFNEATAILAGDALQALAFEI
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCEEEEEECCCEEEEEHHHHHHHHHHH
LQDINYSDIDKLKKINKLFAQYCGASGMVGGQQLDIEGENKKLKIEELQILHTNKTAKMF
HHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEHHHEEEEECCHHHHHH
RACIVLPYILSQNQNSQIEDLLVRLSDLIGLCFQIKDDILDVTKTTNELGKTSAKDIDAN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KSTYVSLMGLKDANKYLLDKKNEITQIITKLKNNNFSSTSLENLIDLVINRNC
CCCEEEEEECCCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]