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The map label for this gene is lpxD [H]

Identifier: 134301448

GI number: 134301448

Start: 351298

End: 352311

Strand: Direct

Name: lpxD [H]

Synonym: FTW_0355

Alternate gene names: 134301448

Gene position: 351298-352311 (Clockwise)

Preceding gene: 134301447

Following gene: 134301449

Centisome position: 18.5

GC content: 36.69

Gene sequence:

>1014_bases
ATGTATAGTTTAGATTTTCTAGCGTCAAAGCTTGATGGTGAGGTTAAGGGCGATAAGAATGTCGAGATAAAAAAAATAGC
TACACTATCACTAGCTGGTGAAGGTGATATTTCTTTTTGTACTAACCCTAAATATTTAAAAGCATTGTCTGAAACAAAAG
CTTCTGCAGTTTTAATAACAGAAGAGGTACTAGAGTTTTGTAATACAAACGCGGTGGTACTATCAAATCCTTATATGGCT
TTAGCAAAGGTTATGGAGCTATTTGATAAATCGCCGCGTCCTGATGGTAAAATTCATAGTAAAGCTGTGATAGCCGCAAG
TGCTATAATTGGTGAAAATGTCACTATAGGCGCTAATGCGGTAGTTGGTGAAAATGTAGTAATTGGCGACAATGTTTATA
TAGGTGCCTGTGCAACTATTGATAATGGTACCAAGATAGGTAATGACACGCTGATAAAGAGCAATGTATCTATAGCTCAC
GATGTTGTGATTGGTACTGGGTGTATTATTCATCAAAATGCAGTAATTGGCTGTGATGGTTTTGGTAATGCAAGAGATGA
GGATGGTAGTTGGACAAAAATTCCTCAGCTAGGAAGAGTAATTATCGAAGATGATGTTGAGATTGGTTCTGGTACAACTG
TTGATAGAGGAGCTATTGATGATACAATAATTAAAAAGGGCGCACGTATTGATAATTTAGTACAGATAGCTCATAATGTA
GTTATCGGTAGAAACACCGCTTTAGCCGGTGTTACAGCTGTTGCAGGTAGTACAACGATTGGTGATAACTGCCTTATTGG
AGGTCAGTCAGCAATAACTGGCCATATTAGTATTTGTGATAATACTATTATAGGTGGTGCATCTAATATTGGTAAGTCAA
TCACTAAGCCAGGAATGTACTATGCTGCGTTTGAAGCTAAACCTAGGATTCAATGGGGTAGGTTCGTAGCTAAATTAGCT
AAGATTGATACTCTAATTACAAAAGTAAAGCAACTAGAAGAAAAAATTAAATAA

Upstream 100 bases:

>100_bases
AACACAAGCTTTAGCGTATGTTAATAATGTTGATGATATATCTAGTCAAGTTGTTGAGTTGATGAATAAAGACTCTGAAT
AATTCTAAGGAGTTTTATTG

Downstream 100 bases:

>100_bases
GTAAGGATTGTTTAATGAGCCAGTTTAATCAAAATAATAAACAGATAGATGTAATGGGGATTAGAAAAATCTTACCTCAC
AGGTATCCTTTTGCACTTTT

Product: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MYSLDFLASKLDGEVKGDKNVEIKKIATLSLAGEGDISFCTNPKYLKALSETKASAVLITEEVLEFCNTNAVVLSNPYMA
LAKVMELFDKSPRPDGKIHSKAVIAASAIIGENVTIGANAVVGENVVIGDNVYIGACATIDNGTKIGNDTLIKSNVSIAH
DVVIGTGCIIHQNAVIGCDGFGNARDEDGSWTKIPQLGRVIIEDDVEIGSGTTVDRGAIDDTIIKKGARIDNLVQIAHNV
VIGRNTALAGVTAVAGSTTIGDNCLIGGQSAITGHISICDNTIIGGASNIGKSITKPGMYYAAFEAKPRIQWGRFVAKLA
KIDTLITKVKQLEEKIK

Sequences:

>Translated_337_residues
MYSLDFLASKLDGEVKGDKNVEIKKIATLSLAGEGDISFCTNPKYLKALSETKASAVLITEEVLEFCNTNAVVLSNPYMA
LAKVMELFDKSPRPDGKIHSKAVIAASAIIGENVTIGANAVVGENVVIGDNVYIGACATIDNGTKIGNDTLIKSNVSIAH
DVVIGTGCIIHQNAVIGCDGFGNARDEDGSWTKIPQLGRVIIEDDVEIGSGTTVDRGAIDDTIIKKGARIDNLVQIAHNV
VIGRNTALAGVTAVAGSTTIGDNCLIGGQSAITGHISICDNTIIGGASNIGKSITKPGMYYAAFEAKPRIQWGRFVAKLA
KIDTLITKVKQLEEKIK
>Mature_337_residues
MYSLDFLASKLDGEVKGDKNVEIKKIATLSLAGEGDISFCTNPKYLKALSETKASAVLITEEVLEFCNTNAVVLSNPYMA
LAKVMELFDKSPRPDGKIHSKAVIAASAIIGENVTIGANAVVGENVVIGDNVYIGACATIDNGTKIGNDTLIKSNVSIAH
DVVIGTGCIIHQNAVIGCDGFGNARDEDGSWTKIPQLGRVIIEDDVEIGSGTTVDRGAIDDTIIKKGARIDNLVQIAHNV
VIGRNTALAGVTAVAGSTTIGDNCLIGGQSAITGHISICDNTIIGGASNIGKSITKPGMYYAAFEAKPRIQWGRFVAKLA
KIDTLITKVKQLEEKIK

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786376, Length=330, Percent_Identity=46.0606060606061, Blast_Score=295, Evalue=3e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573 [H]

Pfam domain/function: PF00132 Hexapep; PF04613 LpxD [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 35428; Mature: 35428

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSLDFLASKLDGEVKGDKNVEIKKIATLSLAGEGDISFCTNPKYLKALSETKASAVLIT
CCCHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCEEECCCHHHHHHHHHCCCEEEEEH
EEVLEFCNTNAVVLSNPYMALAKVMELFDKSPRPDGKIHSKAVIAASAIIGENVTIGANA
HHHHHHHCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCHHHEEEHHHHCCCEEECCCE
VVGENVVIGDNVYIGACATIDNGTKIGNDTLIKSNVSIAHDVVIGTGCIIHQNAVIGCDG
EECCCEEECCCEEEEEEEEECCCCCCCCCCEEECCCCEEEEEEEECCEEEECCEEEEECC
FGNARDEDGSWTKIPQLGRVIIEDDVEIGSGTTVDRGAIDDTIIKKGARIDNLVQIAHNV
CCCCCCCCCCCCCCHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHE
VIGRNTALAGVTAVAGSTTIGDNCLIGGQSAITGHISICDNTIIGGASNIGKSITKPGMY
EECCCCHHHHEEEECCCCCCCCCEEECCCCEEEEEEEEECCCEECCCHHHHHHHCCCCEE
YAAFEAKPRIQWGRFVAKLAKIDTLITKVKQLEEKIK
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYSLDFLASKLDGEVKGDKNVEIKKIATLSLAGEGDISFCTNPKYLKALSETKASAVLIT
CCCHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCEEECCCHHHHHHHHHCCCEEEEEH
EEVLEFCNTNAVVLSNPYMALAKVMELFDKSPRPDGKIHSKAVIAASAIIGENVTIGANA
HHHHHHHCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCHHHEEEHHHHCCCEEECCCE
VVGENVVIGDNVYIGACATIDNGTKIGNDTLIKSNVSIAHDVVIGTGCIIHQNAVIGCDG
EECCCEEECCCEEEEEEEEECCCCCCCCCCEEECCCCEEEEEEEECCEEEECCEEEEECC
FGNARDEDGSWTKIPQLGRVIIEDDVEIGSGTTVDRGAIDDTIIKKGARIDNLVQIAHNV
CCCCCCCCCCCCCCHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHE
VIGRNTALAGVTAVAGSTTIGDNCLIGGQSAITGHISICDNTIIGGASNIGKSITKPGMY
EECCCCHHHHEEEECCCCCCCCCEEECCCCEEEEEEEEECCCEECCCHHHHHHHCCCCEE
YAAFEAKPRIQWGRFVAKLAKIDTLITKVKQLEEKIK
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA