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The map label for this gene is fumA [H]

Identifier: 134301435

GI number: 134301435

Start: 332733

End: 334247

Strand: Direct

Name: fumA [H]

Synonym: FTW_0335

Alternate gene names: 134301435

Gene position: 332733-334247 (Clockwise)

Preceding gene: 134301434

Following gene: 134301436

Centisome position: 17.53

GC content: 38.42

Gene sequence:

>1515_bases
ATGGCTGTTATCAAAGCAGAAGATCTAGTAAATAGTGTTGCAGAAGCATTGCAATATATATCTTATTATCATCCAAAAGA
TTTTATTGATGCAATGTATGAAGCATATCAGCGTGAAGAGTCAAAACCAGCTAAAGATGCAATGGTGCAAATTCTAATAA
ACTCGAGAATGTCTGCTATTGGTAAGCGTCCGATCTGCCAAGATACAGGGATGGTATGCGCTTTTGTCAAAGTTGGCATG
GATGCAAAACTTGACAAGACTGATAGAACAATTACAGAACTTATTAATGAGGGTGTGCGTCGTGGCTATAATGATCCGCA
TAACCCTTTGAGAGCATCTATGGTTTTTCCGCCACATGGATCTAGAAAAAACACTAAAGATAATACTCCAGCAATTGTTC
ATATTGATTTAGTTCATGGTGATAAAATTGAGGTTGATATTGCTGCAAAAGGTGGTGGATCTGAATTTAAATCAAAATTT
AAGGTGTTAAATCCTAGTGATAGCATAATTGATTGGGTTGAAGAGATGTTGCCAACTATGGGGGCTGGCTGGTGTCCACC
TGGAATTATTGGTATAGGTATTGGAGGCACTGCAGAAAAAGCGATACTTTTGGCTAAGGAATCTCTTAGTGAAGAGATTA
ATATCCAAGATATTATCAAAAATGGCCCACAAAATGAAACAGAGCAACTAAGATTAGATATCTATAATCGTGTCAATGCT
CTGGGTATAGGTGCACAAGGCTTAGGTGGTTTGACAACAGTTTTAGATGTCAAAATAAATGAATATCCAACACATGCTGC
GTGTAAGCCAGTTGCTTTGATTCCTAACTGTGCAGCTACTCGCCATGTTCATTTTGTTTTAGATGGTTCTGGTGCCGTTG
ATTTACCAGCTCCTAAGATAGAAGACTGGCCAGTAATTGAGCAAGCACAAAATGATGATGTTAAGAGAATAAATCTTGAT
ACAGTTACTAGAGAAGAGATTGAGGCATTAAGGTCGGGAGATAACGTTTTGATTAGCGGTAAAATCTTAACTGGTCGAGA
TGCTGCACATAAGCGTCTACAAGATATGTATAATGCTGGAGAAGATTTTCCTGTTGATCTTAACGGTAGATTTATCTACT
ACGTAGGTCCTGTTGATCCAGTAGGTGATGAGGTGGTTGGTCCAGCTGGTCCAACTACTGCTACGCGTATGGATAAGTTT
ACGCCATTTATGTTAGAGAAAGCTGGTATCATGGGAATGATAGGTAAATCAGAGCGTGGTCAGGCAACGATTGATTCTAT
CAAGAAAAATAAAGCTATTTACTTCATGGGTGTAGGTGGTGCTGCTTATCTCATATCTAAATCTATCAAAAAAGCAGAAG
TTGTTGCATTTGAAGATCTTGGTATGGAGGCAATTTATGAGTTTGAGGTTGAGGATATGCCAGTAACTGTGGCTGTTGAC
TCTTTAGGTGTTTCTGCTCATCAGCAAGGTCCTAAACTTTGGAAACAAAAAATAGCTGAAATTAATAAGGTCTAA

Upstream 100 bases:

>100_bases
TGATAACTTATACTAAAAGATAAACTAGCTTAAAAATTATACTATTACTGTTATAATAGACAATGAGTATCAGTTTTTTA
AAATAAAGAGGATTAAATAA

Downstream 100 bases:

>100_bases
TCTATGCAAGAGTTAGTATTTTTTATAATTAAGATTTTCTTTGTAATTTTAGCTATTTCTGTAGTTGGCATAGTTAGTGC
TGTAGTTATAAAAAGCTTCA

Product: fumerate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MAVIKAEDLVNSVAEALQYISYYHPKDFIDAMYEAYQREESKPAKDAMVQILINSRMSAIGKRPICQDTGMVCAFVKVGM
DAKLDKTDRTITELINEGVRRGYNDPHNPLRASMVFPPHGSRKNTKDNTPAIVHIDLVHGDKIEVDIAAKGGGSEFKSKF
KVLNPSDSIIDWVEEMLPTMGAGWCPPGIIGIGIGGTAEKAILLAKESLSEEINIQDIIKNGPQNETEQLRLDIYNRVNA
LGIGAQGLGGLTTVLDVKINEYPTHAACKPVALIPNCAATRHVHFVLDGSGAVDLPAPKIEDWPVIEQAQNDDVKRINLD
TVTREEIEALRSGDNVLISGKILTGRDAAHKRLQDMYNAGEDFPVDLNGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKF
TPFMLEKAGIMGMIGKSERGQATIDSIKKNKAIYFMGVGGAAYLISKSIKKAEVVAFEDLGMEAIYEFEVEDMPVTVAVD
SLGVSAHQQGPKLWKQKIAEINKV

Sequences:

>Translated_504_residues
MAVIKAEDLVNSVAEALQYISYYHPKDFIDAMYEAYQREESKPAKDAMVQILINSRMSAIGKRPICQDTGMVCAFVKVGM
DAKLDKTDRTITELINEGVRRGYNDPHNPLRASMVFPPHGSRKNTKDNTPAIVHIDLVHGDKIEVDIAAKGGGSEFKSKF
KVLNPSDSIIDWVEEMLPTMGAGWCPPGIIGIGIGGTAEKAILLAKESLSEEINIQDIIKNGPQNETEQLRLDIYNRVNA
LGIGAQGLGGLTTVLDVKINEYPTHAACKPVALIPNCAATRHVHFVLDGSGAVDLPAPKIEDWPVIEQAQNDDVKRINLD
TVTREEIEALRSGDNVLISGKILTGRDAAHKRLQDMYNAGEDFPVDLNGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKF
TPFMLEKAGIMGMIGKSERGQATIDSIKKNKAIYFMGVGGAAYLISKSIKKAEVVAFEDLGMEAIYEFEVEDMPVTVAVD
SLGVSAHQQGPKLWKQKIAEINKV
>Mature_503_residues
AVIKAEDLVNSVAEALQYISYYHPKDFIDAMYEAYQREESKPAKDAMVQILINSRMSAIGKRPICQDTGMVCAFVKVGMD
AKLDKTDRTITELINEGVRRGYNDPHNPLRASMVFPPHGSRKNTKDNTPAIVHIDLVHGDKIEVDIAAKGGGSEFKSKFK
VLNPSDSIIDWVEEMLPTMGAGWCPPGIIGIGIGGTAEKAILLAKESLSEEINIQDIIKNGPQNETEQLRLDIYNRVNAL
GIGAQGLGGLTTVLDVKINEYPTHAACKPVALIPNCAATRHVHFVLDGSGAVDLPAPKIEDWPVIEQAQNDDVKRINLDT
VTREEIEALRSGDNVLISGKILTGRDAAHKRLQDMYNAGEDFPVDLNGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFT
PFMLEKAGIMGMIGKSERGQATIDSIKKNKAIYFMGVGGAAYLISKSIKKAEVVAFEDLGMEAIYEFEVEDMPVTVAVDS
LGVSAHQQGPKLWKQKIAEINKV

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=470, Percent_Identity=31.063829787234, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787897, Length=470, Percent_Identity=30.8510638297872, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789442, Length=279, Percent_Identity=28.3154121863799, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789443, Length=189, Percent_Identity=30.6878306878307, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54975; Mature: 54843

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIKAEDLVNSVAEALQYISYYHPKDFIDAMYEAYQREESKPAKDAMVQILINSRMSAI
CCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GKRPICQDTGMVCAFVKVGMDAKLDKTDRTITELINEGVRRGYNDPHNPLRASMVFPPHG
CCCCCCCCCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
SRKNTKDNTPAIVHIDLVHGDKIEVDIAAKGGGSEFKSKFKVLNPSDSIIDWVEEMLPTM
CCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCHHHHHHEEEECCCHHHHHHHHHHHHHC
GAGWCPPGIIGIGIGGTAEKAILLAKESLSEEINIQDIIKNGPQNETEQLRLDIYNRVNA
CCCCCCCCEEEECCCCCCHHEEEEEHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCE
LGIGAQGLGGLTTVLDVKINEYPTHAACKPVALIPNCAATRHVHFVLDGSGAVDLPAPKI
EECCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCC
EDWPVIEQAQNDDVKRINLDTVTREEIEALRSGDNVLISGKILTGRDAAHKRLQDMYNAG
CCCCCHHCCCCCCCEEEECCHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHCC
EDFPVDLNGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTPFMLEKAGIMGMIGKSERG
CCCEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHCCCEEECCCCCCC
QATIDSIKKNKAIYFMGVGGAAYLISKSIKKAEVVAFEDLGMEAIYEFEVEDMPVTVAVD
CHHHHHHHCCCEEEEEECCHHHHHHHHHCCHHHEEEEHHCCCEEEEEEEECCCCEEEEEE
SLGVSAHQQGPKLWKQKIAEINKV
CCCCCHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVIKAEDLVNSVAEALQYISYYHPKDFIDAMYEAYQREESKPAKDAMVQILINSRMSAI
CEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GKRPICQDTGMVCAFVKVGMDAKLDKTDRTITELINEGVRRGYNDPHNPLRASMVFPPHG
CCCCCCCCCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
SRKNTKDNTPAIVHIDLVHGDKIEVDIAAKGGGSEFKSKFKVLNPSDSIIDWVEEMLPTM
CCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCHHHHHHEEEECCCHHHHHHHHHHHHHC
GAGWCPPGIIGIGIGGTAEKAILLAKESLSEEINIQDIIKNGPQNETEQLRLDIYNRVNA
CCCCCCCCEEEECCCCCCHHEEEEEHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCE
LGIGAQGLGGLTTVLDVKINEYPTHAACKPVALIPNCAATRHVHFVLDGSGAVDLPAPKI
EECCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCC
EDWPVIEQAQNDDVKRINLDTVTREEIEALRSGDNVLISGKILTGRDAAHKRLQDMYNAG
CCCCCHHCCCCCCCEEEECCHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHCC
EDFPVDLNGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTPFMLEKAGIMGMIGKSERG
CCCEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHCCCEEECCCCCCC
QATIDSIKKNKAIYFMGVGGAAYLISKSIKKAEVVAFEDLGMEAIYEFEVEDMPVTVAVD
CHHHHHHHCCCEEEEEECCHHHHHHHHHCCHHHEEEEHHCCCEEEEEEEECCCCEEEEEE
SLGVSAHQQGPKLWKQKIAEINKV
CCCCCHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]