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The map label for this gene is cysL [H]

Identifier: 134301415

GI number: 134301415

Start: 315218

End: 316210

Strand: Direct

Name: cysL [H]

Synonym: FTW_0314

Alternate gene names: 134301415

Gene position: 315218-316210 (Clockwise)

Preceding gene: 134301413

Following gene: 134301416

Centisome position: 16.6

GC content: 30.82

Gene sequence:

>993_bases
ATGAGCCGGAGAATTACCCTAAAGCAACTTAATGTTTTCGTTGAGACTGCAATGAATAAATCTATAACTATAGCTGCAGA
AAAATGTTATATAACTCAGCCTTCTGCTAGTAAAGCTATATTACAATTAGAAGAAGCTTTGCAAAAAGTTTTATTTGAAA
GAGGTGCTGGCAAAGAAATTAGTTTAACATATGAAGGAGAACTTTTATTTGTTGAAGCCAAAAGTATACTTGATCGGGTC
TCTGAGTTAATAAAAGATAAAGAAAATATTACTGGTAAAATAGTTATTGGTGCTAGTGTAACAATATGTGATTATGTATT
ACCGAAAATATTACCGCAGTTTCAAAAATTATTTCCAAAAATCAATATAAGAATTGAGAGAGTGCTCTCAGATAGAATCA
GATATTATGTTGAAAATGCTGTATGTGATATAGGTTTAGTTGAAGGTAGGGTAATATCTAGAGAGTTGGAGCAAACATTA
TGGAAAGAAGATCGTATTGAAATAATTTGTGCTAAAAATCATGAATTAGCGAAGAAAGATAAAGTGTCATTAGAAGAGGT
CTTAGTAGGTCAGAATTGGGTACTATGGCATCATGATATGGGTACTAGAGATATTTACATACAAACTAAAGGAAGAGAAA
AGCTAACCCAAAGCTTTGGATTTGGTGCTGAAGACCTTGGTAATGGATTATTAAAATTTAGTGAGGTTGCTTGGGATAAT
CAAGAGAGTATCACTAGGGCTTCTACTAACTATATTTCTAGTAGTATTGTTTTTGATAGTGCTGAGGCAATTAAAAATTA
TGTTGCTAATAGTAATTTTCTTGCTAGTTTATCAAAAACAATGTTGGATAATACCCTTTGCAATGATATTAAGATTCTTA
ATGTAGAAGGCGGATATCAGACAAGAGATTTTTATATATTAAAACATAAGCAAAGATTTTTTTCTCAAGCTTCAGATGTC
TTTTTAGATTGGATTTCAAATTATAATCTATAG

Upstream 100 bases:

>100_bases
TATTAAAAATAACGGCGTATTAAGGACAATATTTATTGAATAGTTAAAGTTAGAGTTATATATTTACAGTCAAGTTTTAT
ATTTTGTTTCATAGGATCAA

Downstream 100 bases:

>100_bases
CATTGATAGTTATTTAATTAAAACTTTACATGCAACTAAATACTTATATGTAAGCTATCTAAAGTATCATAGTAATAAAT
AGCCTTATATATTAAAAAAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSRRITLKQLNVFVETAMNKSITIAAEKCYITQPSASKAILQLEEALQKVLFERGAGKEISLTYEGELLFVEAKSILDRV
SELIKDKENITGKIVIGASVTICDYVLPKILPQFQKLFPKINIRIERVLSDRIRYYVENAVCDIGLVEGRVISRELEQTL
WKEDRIEIICAKNHELAKKDKVSLEEVLVGQNWVLWHHDMGTRDIYIQTKGREKLTQSFGFGAEDLGNGLLKFSEVAWDN
QESITRASTNYISSSIVFDSAEAIKNYVANSNFLASLSKTMLDNTLCNDIKILNVEGGYQTRDFYILKHKQRFFSQASDV
FLDWISNYNL

Sequences:

>Translated_330_residues
MSRRITLKQLNVFVETAMNKSITIAAEKCYITQPSASKAILQLEEALQKVLFERGAGKEISLTYEGELLFVEAKSILDRV
SELIKDKENITGKIVIGASVTICDYVLPKILPQFQKLFPKINIRIERVLSDRIRYYVENAVCDIGLVEGRVISRELEQTL
WKEDRIEIICAKNHELAKKDKVSLEEVLVGQNWVLWHHDMGTRDIYIQTKGREKLTQSFGFGAEDLGNGLLKFSEVAWDN
QESITRASTNYISSSIVFDSAEAIKNYVANSNFLASLSKTMLDNTLCNDIKILNVEGGYQTRDFYILKHKQRFFSQASDV
FLDWISNYNL
>Mature_329_residues
SRRITLKQLNVFVETAMNKSITIAAEKCYITQPSASKAILQLEEALQKVLFERGAGKEISLTYEGELLFVEAKSILDRVS
ELIKDKENITGKIVIGASVTICDYVLPKILPQFQKLFPKINIRIERVLSDRIRYYVENAVCDIGLVEGRVISRELEQTLW
KEDRIEIICAKNHELAKKDKVSLEEVLVGQNWVLWHHDMGTRDIYIQTKGREKLTQSFGFGAEDLGNGLLKFSEVAWDNQ
ESITRASTNYISSSIVFDSAEAIKNYVANSNFLASLSKTMLDNTLCNDIKILNVEGGYQTRDFYILKHKQRFFSQASDVF
LDWISNYNL

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=329, Percent_Identity=27.0516717325228, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI157672245, Length=215, Percent_Identity=28.3720930232558, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI145693105, Length=204, Percent_Identity=27.9411764705882, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081904, Length=223, Percent_Identity=27.8026905829596, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787601, Length=129, Percent_Identity=31.7829457364341, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790399, Length=123, Percent_Identity=30.0813008130081, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788748, Length=210, Percent_Identity=27.6190476190476, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788887, Length=195, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788296, Length=222, Percent_Identity=22.5225225225225, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37683; Mature: 37552

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRITLKQLNVFVETAMNKSITIAAEKCYITQPSASKAILQLEEALQKVLFERGAGKEI
CCCCEEHHHHHHHHHHHCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEE
SLTYEGELLFVEAKSILDRVSELIKDKENITGKIVIGASVTICDYVLPKILPQFQKLFPK
EEEECCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCH
INIRIERVLSDRIRYYVENAVCDIGLVEGRVISRELEQTLWKEDRIEIICAKNHELAKKD
HCHHHHHHHHHHHHHHHHHHHHCEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHH
KVSLEEVLVGQNWVLWHHDMGTRDIYIQTKGREKLTQSFGFGAEDLGNGLLKFSEVAWDN
CCCHHHHHCCCCEEEEEECCCCEEEEEEECCHHHHHHHHCCCHHHHCCHHHHHHHHCCCC
QESITRASTNYISSSIVFDSAEAIKNYVANSNFLASLSKTMLDNTLCNDIKILNVEGGYQ
HHHHHHHHHHHHHHHHEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCC
TRDFYILKHKQRFFSQASDVFLDWISNYNL
CCEEEEEEHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRRITLKQLNVFVETAMNKSITIAAEKCYITQPSASKAILQLEEALQKVLFERGAGKEI
CCCEEHHHHHHHHHHHCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEE
SLTYEGELLFVEAKSILDRVSELIKDKENITGKIVIGASVTICDYVLPKILPQFQKLFPK
EEEECCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCH
INIRIERVLSDRIRYYVENAVCDIGLVEGRVISRELEQTLWKEDRIEIICAKNHELAKKD
HCHHHHHHHHHHHHHHHHHHHHCEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHH
KVSLEEVLVGQNWVLWHHDMGTRDIYIQTKGREKLTQSFGFGAEDLGNGLLKFSEVAWDN
CCCHHHHHCCCCEEEEEECCCCEEEEEEECCHHHHHHHHCCCHHHHCCHHHHHHHHCCCC
QESITRASTNYISSSIVFDSAEAIKNYVANSNFLASLSKTMLDNTLCNDIKILNVEGGYQ
HHHHHHHHHHHHHHHHEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCC
TRDFYILKHKQRFFSQASDVFLDWISNYNL
CCEEEEEEHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]