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The map label for this gene is metK [H]

Identifier: 134301358

GI number: 134301358

Start: 250606

End: 251766

Strand: Reverse

Name: metK [H]

Synonym: FTW_0239

Alternate gene names: 134301358

Gene position: 251766-250606 (Counterclockwise)

Preceding gene: 134301366

Following gene: 134301337

Centisome position: 13.26

GC content: 35.66

Gene sequence:

>1161_bases
ATGTCAAAAAATTACCTATTTACTTCTGAATCTGTATCAGAAGGACATCCAGATAAACTAGCTGACCAAATATCAGACGC
AATCCTAGATGAGATTTTAAAACAAGATAAAAATGCTCGTGTAGCATGTGAAACTTTAGTAAAAACTGGTATGGCTTTAG
TTGCTGGAGAAATTACAACATCTGCTTGGGTAGATATCAAAGAATTAGTAAGAAATGTTATTACAGAAACAGGCTATGAT
AATGCTAGTAAAGGTATTGATGGTAGAACTTGTTCTGTAATTAATGCTATTGATAAACAGTCTAGAGATATTACCCAAGG
CGTAGATCGTGGCTCTCTAGAAGATCTTGGTGCTGGTGACCAAGGATTAATGTTTGGATTTGCTACAAATGAGACACCAA
CACTAATGCCATCAGCCATTTACTACTCTCACCTATTGATGAGAAAACAAGCAGAACTTAGAAAATCAGGGAAGCTTGCA
TGGTTAAGACCAGATGCAAAAGCTCAAGTTACATTAGCTTACGAAAATGATAAGCCTAAATTCATTGATACTATAGTACT
ATCTACACAACATAATGAATCAATCTCTCAAAAAGAATTGCATGATGCTGTGATTGAGGAAATTGTTAAAAAGGTTATTC
CTAACGAACTAATTACTAAAGATACCAAATATCATATTAACCCAACAGGTGTATTTTTAATTGGTGGTCCTCAAGGCGAT
TGTGGCCTTACAGGAAGAAAAATTATTGTTGATACTTATGGCGGTGCTGCTCATCATGGTGGTGGTGCTTTCTCTGGCAA
AGATCCTTCTAAAGTTGATCGCTCAGGGGCATATATGGGTAGATATATAGCTAAAAACATTGTTGCTGCTGGTCTAGCTG
ACAAGTGTGAAGTACAAGTAGCGTATGCAATTGGTGTAGCAAAACCAGTTTCCCTTATGGTTAATACTTTTGGTACTGGC
AAAATTACAGATAATCAGATAGAAAAGCTCGTAGCTGAAGTTTTTGATCTTAGAGTTGGTAAAATTATCGAAAACCTTGA
CTTATTAAGACCAATTTATCGCAAAACTTCTAACTATGGTCATTTTGGTCGCGAGTTACCAGAGTTTACTTGGGAAAAAA
TTGATAAAGCTGATATTCTTAAATCAGCAGCCAGAATATAA

Upstream 100 bases:

>100_bases
AACTTGCTTGCGACATTAAATAATAAGCTAAAGCGTTTCTACCAAATTTTTACCTAAAAAATTTTCTAGAAGCCTTTTAG
CTAACTAAAAGGAGCATAAA

Downstream 100 bases:

>100_bases
CTAACTAGACACAAAAATGTCATACTGAGTTTATTTCAGCAGCTTATTATAAGTATTTAATTGAGATATTTAAGTTACTA
GTTTTATTTAGAATAAATCA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSKNYLFTSESVSEGHPDKLADQISDAILDEILKQDKNARVACETLVKTGMALVAGEITTSAWVDIKELVRNVITETGYD
NASKGIDGRTCSVINAIDKQSRDITQGVDRGSLEDLGAGDQGLMFGFATNETPTLMPSAIYYSHLLMRKQAELRKSGKLA
WLRPDAKAQVTLAYENDKPKFIDTIVLSTQHNESISQKELHDAVIEEIVKKVIPNELITKDTKYHINPTGVFLIGGPQGD
CGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSGAYMGRYIAKNIVAAGLADKCEVQVAYAIGVAKPVSLMVNTFGTG
KITDNQIEKLVAEVFDLRVGKIIENLDLLRPIYRKTSNYGHFGRELPEFTWEKIDKADILKSAARI

Sequences:

>Translated_386_residues
MSKNYLFTSESVSEGHPDKLADQISDAILDEILKQDKNARVACETLVKTGMALVAGEITTSAWVDIKELVRNVITETGYD
NASKGIDGRTCSVINAIDKQSRDITQGVDRGSLEDLGAGDQGLMFGFATNETPTLMPSAIYYSHLLMRKQAELRKSGKLA
WLRPDAKAQVTLAYENDKPKFIDTIVLSTQHNESISQKELHDAVIEEIVKKVIPNELITKDTKYHINPTGVFLIGGPQGD
CGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSGAYMGRYIAKNIVAAGLADKCEVQVAYAIGVAKPVSLMVNTFGTG
KITDNQIEKLVAEVFDLRVGKIIENLDLLRPIYRKTSNYGHFGRELPEFTWEKIDKADILKSAARI
>Mature_385_residues
SKNYLFTSESVSEGHPDKLADQISDAILDEILKQDKNARVACETLVKTGMALVAGEITTSAWVDIKELVRNVITETGYDN
ASKGIDGRTCSVINAIDKQSRDITQGVDRGSLEDLGAGDQGLMFGFATNETPTLMPSAIYYSHLLMRKQAELRKSGKLAW
LRPDAKAQVTLAYENDKPKFIDTIVLSTQHNESISQKELHDAVIEEIVKKVIPNELITKDTKYHINPTGVFLIGGPQGDC
GLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSGAYMGRYIAKNIVAAGLADKCEVQVAYAIGVAKPVSLMVNTFGTGK
ITDNQIEKLVAEVFDLRVGKIIENLDLLRPIYRKTSNYGHFGRELPEFTWEKIDKADILKSAARI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=55.8823529411765, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI5174529, Length=375, Percent_Identity=54.6666666666667, Blast_Score=404, Evalue=1e-113,
Organism=Escherichia coli, GI1789311, Length=381, Percent_Identity=66.1417322834646, Blast_Score=528, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17538498, Length=375, Percent_Identity=57.3333333333333, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25145633, Length=387, Percent_Identity=56.3307493540052, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17538494, Length=380, Percent_Identity=56.578947368421, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17551082, Length=378, Percent_Identity=53.4391534391534, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32565911, Length=329, Percent_Identity=55.0151975683891, Blast_Score=345, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI32565905, Length=380, Percent_Identity=50, Blast_Score=344, Evalue=5e-95,
Organism=Caenorhabditis elegans, GI25145635, Length=336, Percent_Identity=53.5714285714286, Blast_Score=342, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI32565903, Length=302, Percent_Identity=53.3112582781457, Blast_Score=304, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=64.367816091954, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320710, Length=383, Percent_Identity=55.0913838120104, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=54.2328042328042, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45552159, Length=380, Percent_Identity=54.2105263157895, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580529, Length=380, Percent_Identity=54.2105263157895, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580537, Length=380, Percent_Identity=54.2105263157895, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580533, Length=380, Percent_Identity=54.2105263157895, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580535, Length=380, Percent_Identity=54.2105263157895, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580531, Length=380, Percent_Identity=54.2105263157895, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580527, Length=380, Percent_Identity=53.9473684210526, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580525, Length=380, Percent_Identity=53.9473684210526, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580541, Length=381, Percent_Identity=51.7060367454068, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580539, Length=118, Percent_Identity=58.4745762711864, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42191; Mature: 42059

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNYLFTSESVSEGHPDKLADQISDAILDEILKQDKNARVACETLVKTGMALVAGEITT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHH
SAWVDIKELVRNVITETGYDNASKGIDGRTCSVINAIDKQSRDITQGVDRGSLEDLGAGD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCC
QGLMFGFATNETPTLMPSAIYYSHLLMRKQAELRKSGKLAWLRPDAKAQVTLAYENDKPK
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCCC
FIDTIVLSTQHNESISQKELHDAVIEEIVKKVIPNELITKDTKYHINPTGVFLIGGPQGD
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEECCCEEEEEECCCCC
CGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSGAYMGRYIAKNIVAAGLADKCEVQV
CCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEE
AYAIGVAKPVSLMVNTFGTGKITDNQIEKLVAEVFDLRVGKIIENLDLLRPIYRKTSNYG
EEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HFGRELPEFTWEKIDKADILKSAARI
HHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
SKNYLFTSESVSEGHPDKLADQISDAILDEILKQDKNARVACETLVKTGMALVAGEITT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHH
SAWVDIKELVRNVITETGYDNASKGIDGRTCSVINAIDKQSRDITQGVDRGSLEDLGAGD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCC
QGLMFGFATNETPTLMPSAIYYSHLLMRKQAELRKSGKLAWLRPDAKAQVTLAYENDKPK
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCCC
FIDTIVLSTQHNESISQKELHDAVIEEIVKKVIPNELITKDTKYHINPTGVFLIGGPQGD
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEECCCEEEEEECCCCC
CGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSGAYMGRYIAKNIVAAGLADKCEVQV
CCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEE
AYAIGVAKPVSLMVNTFGTGKITDNQIEKLVAEVFDLRVGKIIENLDLLRPIYRKTSNYG
EEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HFGRELPEFTWEKIDKADILKSAARI
HHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA