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The map label for this gene is glpF [H]

Identifier: 134301342

GI number: 134301342

Start: 230660

End: 231424

Strand: Direct

Name: glpF [H]

Synonym: FTW_0223

Alternate gene names: 134301342

Gene position: 230660-231424 (Clockwise)

Preceding gene: 134301341

Following gene: 134301343

Centisome position: 12.15

GC content: 38.82

Gene sequence:

>765_bases
ATGTTAACAGCATGTATAGCAGAATTAATAGGCACAATGCTACTTATCTTATTGGGTAATGGTGTTGTTGCTGGAGTAGT
TTTAAATAAGACAAAAGCACATGATGGTGGCTGGATAGTTATTACATTTGCTTGGGGTCTAGCTGTTTTTATAGGTGTGT
TAGTTGCTGGACCGATTAGTGGTGCTCACTTGAATCCTGTTGTAACATTATCTTTAGCTCTTGCGGGTAAGTTTTCATGG
GCTTGGGTATTACCATTTATTATAAGTCAGATTATCGGAGCGATGCTTGGTCAACTATTAGTGTGGATTATGTATTATCC
TCATTATTCAGCAACTGATAATCCTGAACTAAAATTAGCGACTTGTTGCACATCACCAGCAATTAAGCATTTGCCAGCGA
ATTTTGCTAGTGAAGTAATTGGAACATTTGTTCTAGTACTAGCTATTTTGACTATGCATGGTGTTGTCTTACATGTCGGA
GACTCTAGTATAACAACAACTTATCCAGTAGATATGGGCGCTCTAGGGGGTATACCAGTAGCATTTGTGGTTGTTGTAAT
CGGCATGTCCTTAGGTGGGACTACAGGTTATGCAATTAATCCAGCTAGAGATTTTGGCCCAAGACTATTTCATGCTATCA
TGCCAATACAAGGAAAAGGCTCTTCACAATGGAGTTATGCTTGGGTACCGATACTTGGTCCAGTGTGTGGAAGTATAATT
GCCACAGCTTTTTATCTATTGTTAAAGGTAAAAGGGGTTTTTTAG

Upstream 100 bases:

>100_bases
GCTTGGTAAAGATGAGGTTTGGCAAAACCAGCAAATTGATAGTTTCATAGAATTTTCTAAAAACTTTAATGTTGAAGAGC
TGTATAAGTAGAGGAAGTTT

Downstream 100 bases:

>100_bases
ATAATGTGTGAAATAACTTTTATACCACTTTTAGCAGATGATGTTTTAAGATTGTTTATTTCTTTTATATTATTTTTAAG
TTGCGTGATTTTAAGTTATA

Product: major intrinsic protein family permease

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLTACIAELIGTMLLILLGNGVVAGVVLNKTKAHDGGWIVITFAWGLAVFIGVLVAGPISGAHLNPVVTLSLALAGKFSW
AWVLPFIISQIIGAMLGQLLVWIMYYPHYSATDNPELKLATCCTSPAIKHLPANFASEVIGTFVLVLAILTMHGVVLHVG
DSSITTTYPVDMGALGGIPVAFVVVVIGMSLGGTTGYAINPARDFGPRLFHAIMPIQGKGSSQWSYAWVPILGPVCGSII
ATAFYLLLKVKGVF

Sequences:

>Translated_254_residues
MLTACIAELIGTMLLILLGNGVVAGVVLNKTKAHDGGWIVITFAWGLAVFIGVLVAGPISGAHLNPVVTLSLALAGKFSW
AWVLPFIISQIIGAMLGQLLVWIMYYPHYSATDNPELKLATCCTSPAIKHLPANFASEVIGTFVLVLAILTMHGVVLHVG
DSSITTTYPVDMGALGGIPVAFVVVVIGMSLGGTTGYAINPARDFGPRLFHAIMPIQGKGSSQWSYAWVPILGPVCGSII
ATAFYLLLKVKGVF
>Mature_254_residues
MLTACIAELIGTMLLILLGNGVVAGVVLNKTKAHDGGWIVITFAWGLAVFIGVLVAGPISGAHLNPVVTLSLALAGKFSW
AWVLPFIISQIIGAMLGQLLVWIMYYPHYSATDNPELKLATCCTSPAIKHLPANFASEVIGTFVLVLAILTMHGVVLHVG
DSSITTTYPVDMGALGGIPVAFVVVVIGMSLGGTTGYAINPARDFGPRLFHAIMPIQGKGSSQWSYAWVPILGPVCGSII
ATAFYLLLKVKGVF

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=263, Percent_Identity=36.5019011406844, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI157266307, Length=259, Percent_Identity=33.5907335907336, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI22538420, Length=257, Percent_Identity=29.5719844357977, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI4502187, Length=260, Percent_Identity=29.2307692307692, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI45446752, Length=225, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI310133356, Length=230, Percent_Identity=27.8260869565217, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI310114181, Length=230, Percent_Identity=27.8260869565217, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1790362, Length=259, Percent_Identity=34.7490347490347, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71994009, Length=249, Percent_Identity=31.3253012048193, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17544068, Length=257, Percent_Identity=33.0739299610895, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17531431, Length=257, Percent_Identity=30.7392996108949, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531429, Length=257, Percent_Identity=30.7392996108949, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17558372, Length=253, Percent_Identity=28.4584980237154, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI32564052, Length=249, Percent_Identity=30.5220883534137, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17533613, Length=249, Percent_Identity=30.5220883534137, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71992966, Length=258, Percent_Identity=29.4573643410853, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71992961, Length=251, Percent_Identity=29.0836653386454, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71993722, Length=257, Percent_Identity=28.7937743190661, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321054, Length=221, Percent_Identity=32.579185520362, Blast_Score=102, Evalue=7e-23,
Organism=Drosophila melanogaster, GI45550503, Length=248, Percent_Identity=27.0161290322581, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24762342, Length=206, Percent_Identity=25.7281553398058, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 26701; Mature: 26701

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTACIAELIGTMLLILLGNGVVAGVVLNKTKAHDGGWIVITFAWGLAVFIGVLVAGPIS
CHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHCCCC
GAHLNPVVTLSLALAGKFSWAWVLPFIISQIIGAMLGQLLVWIMYYPHYSATDNPELKLA
CCCCCHHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
TCCTSPAIKHLPANFASEVIGTFVLVLAILTMHGVVLHVGDSSITTTYPVDMGALGGIPV
EECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCCCCCHHH
AFVVVVIGMSLGGTTGYAINPARDFGPRLFHAIMPIQGKGSSQWSYAWVPILGPVCGSII
HHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHH
ATAFYLLLKVKGVF
HHHHHHHHHHHCCC
>Mature Secondary Structure
MLTACIAELIGTMLLILLGNGVVAGVVLNKTKAHDGGWIVITFAWGLAVFIGVLVAGPIS
CHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHCCCC
GAHLNPVVTLSLALAGKFSWAWVLPFIISQIIGAMLGQLLVWIMYYPHYSATDNPELKLA
CCCCCHHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
TCCTSPAIKHLPANFASEVIGTFVLVLAILTMHGVVLHVGDSSITTTYPVDMGALGGIPV
EECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCCCCCHHH
AFVVVVIGMSLGGTTGYAINPARDFGPRLFHAIMPIQGKGSSQWSYAWVPILGPVCGSII
HHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHH
ATAFYLLLKVKGVF
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]