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The map label for this gene is cca [H]

Identifier: 134301319

GI number: 134301319

Start: 198874

End: 199956

Strand: Reverse

Name: cca [H]

Synonym: FTW_0193

Alternate gene names: 134301319

Gene position: 199956-198874 (Counterclockwise)

Preceding gene: 134301335

Following gene: 134301318

Centisome position: 10.53

GC content: 32.69

Gene sequence:

>1083_bases
ATGAAATTTTACTTAGTTGGTGGCGCCGTTAGAGATATGTTGTTAGGCATAACACCTAAAGACAAGGATTGGGTAGTTGT
TGGTGCAACTGAAGACGAGATGCTAGCAAATGGATTTATAAAAATAGCAGCCAACTTCCCAGTTTTTATCCACCCACAAA
CCAAACAAGAGTATGCTTTAGCAAGGTCAGAAAAGAAAACTGCCAGTGGCTATCATGGCTTTGAAGTTAATTTTTCAAAA
TATATAACTCTAGAAGACGATCTTAAACGTCGTGATCTCACAATTAACTCAATAGCAATCGATCAAAATAATAAAGTAAT
TGACCCTTTTAATGGCCAAGCCGATCTTCAAAATAGAATTTTACGCCATACTTCAATAGCTTTTATCGAAGACCCACTGC
GTGTAGTTAGACTTGCGCGTTTCAAGGCTCAACTTAGTAACTTCAATTTCTCAATAGCCCAAGAAATGCTGGCACTTATA
AAAGAGTTAGTTAAAACAGGTGAATTAAATCATTTAACGCGTGAAAGATTACATATAGAGTTTGTCAAAGCTCTTAACAA
TCCCAAGATCTTCTTTACGACACTCAAAGAACTAGAAGCTTTAAAAATAATATTTCCAAATATCAGCTGCATTTTACCAC
TAATACCAAATAAATCTTTTTTTGAAAACCCCATCTATAAAGGCTCTAATATCAATGAAAAAATAACGCTATGTTTACTC
AAGATCCCACAACAACAATTAGATGATATCAGAAAGGAACTTTTATTAACAAATAAACACTACAAACTTTTAAAAGCAAG
CATAGCTATCAGCAAAATACTCGAAGATAGAAGTATCACTGCTGAAGAGATATTTCAACTAATCAAAAACGCTAATATTA
TCCGTGACAAAAATTTATTTGCCGAGAGTTTAAATCTCTATAAAAAATATCTAAAGATATGCGATACTATAACTCCACAT
AGGAATTATCAACTATTACAAACTACCATTAATACAATTAAAAATGCAAGCATTGATAGTTTGACGATAAAAACAATACC
TAAAGATAAACTCAGAAACACGCTAAAGCAGCTAAAACTATGA

Upstream 100 bases:

>100_bases
ATTTATAAAGTTGTTAAAAAAGTGACACGAATAACAAATAATTATACACTTTTTTCTGCTAACATAAAGTAATCAAAGAG
ATGTTTAACTTATTTAAGCA

Downstream 100 bases:

>100_bases
TACAATTACAAAATTGTTTTTTATAGTGTTACATATCTAATGAAAAAACTAAGCAATTGTATATTTATATTTAATACATT
AGCTTGCTTTGTAGCTTTAG

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKFYLVGGAVRDMLLGITPKDKDWVVVGATEDEMLANGFIKIAANFPVFIHPQTKQEYALARSEKKTASGYHGFEVNFSK
YITLEDDLKRRDLTINSIAIDQNNKVIDPFNGQADLQNRILRHTSIAFIEDPLRVVRLARFKAQLSNFNFSIAQEMLALI
KELVKTGELNHLTRERLHIEFVKALNNPKIFFTTLKELEALKIIFPNISCILPLIPNKSFFENPIYKGSNINEKITLCLL
KIPQQQLDDIRKELLLTNKHYKLLKASIAISKILEDRSITAEEIFQLIKNANIIRDKNLFAESLNLYKKYLKICDTITPH
RNYQLLQTTINTIKNASIDSLTIKTIPKDKLRNTLKQLKL

Sequences:

>Translated_360_residues
MKFYLVGGAVRDMLLGITPKDKDWVVVGATEDEMLANGFIKIAANFPVFIHPQTKQEYALARSEKKTASGYHGFEVNFSK
YITLEDDLKRRDLTINSIAIDQNNKVIDPFNGQADLQNRILRHTSIAFIEDPLRVVRLARFKAQLSNFNFSIAQEMLALI
KELVKTGELNHLTRERLHIEFVKALNNPKIFFTTLKELEALKIIFPNISCILPLIPNKSFFENPIYKGSNINEKITLCLL
KIPQQQLDDIRKELLLTNKHYKLLKASIAISKILEDRSITAEEIFQLIKNANIIRDKNLFAESLNLYKKYLKICDTITPH
RNYQLLQTTINTIKNASIDSLTIKTIPKDKLRNTLKQLKL
>Mature_360_residues
MKFYLVGGAVRDMLLGITPKDKDWVVVGATEDEMLANGFIKIAANFPVFIHPQTKQEYALARSEKKTASGYHGFEVNFSK
YITLEDDLKRRDLTINSIAIDQNNKVIDPFNGQADLQNRILRHTSIAFIEDPLRVVRLARFKAQLSNFNFSIAQEMLALI
KELVKTGELNHLTRERLHIEFVKALNNPKIFFTTLKELEALKIIFPNISCILPLIPNKSFFENPIYKGSNINEKITLCLL
KIPQQQLDDIRKELLLTNKHYKLLKASIAISKILEDRSITAEEIFQLIKNANIIRDKNLFAESLNLYKKYLKICDTITPH
RNYQLLQTTINTIKNASIDSLTIKTIPKDKLRNTLKQLKL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=361, Percent_Identity=42.6592797783933, Blast_Score=268, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6321016, Length=214, Percent_Identity=31.3084112149533, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 41396; Mature: 41396

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFYLVGGAVRDMLLGITPKDKDWVVVGATEDEMLANGFIKIAANFPVFIHPQTKQEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCEEEEEECCCEEECCCCHHHHHH
ARSEKKTASGYHGFEVNFSKYITLEDDLKRRDLTINSIAIDQNNKVIDPFNGQADLQNRI
HHHCCCCCCCCCCEEEECEEEEEECCCCHHCCEEEEEEEEECCCCEECCCCCCHHHHHHH
LRHTSIAFIEDPLRVVRLARFKAQLSNFNFSIAQEMLALIKELVKTGELNHLTRERLHIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FVKALNNPKIFFTTLKELEALKIIFPNISCILPLIPNKSFFENPIYKGSNINEKITLCLL
HHHHCCCCEEEEEEHHHHHHHEEECCCCEEEEEECCCCHHHCCCCCCCCCCCCCEEEEEE
KIPQQQLDDIRKELLLTNKHYKLLKASIAISKILEDRSITAEEIFQLIKNANIIRDKNLF
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECHHHH
AESLNLYKKYLKICDTITPHRNYQLLQTTINTIKNASIDSLTIKTIPKDKLRNTLKQLKL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKFYLVGGAVRDMLLGITPKDKDWVVVGATEDEMLANGFIKIAANFPVFIHPQTKQEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCEEEEEECCCEEECCCCHHHHHH
ARSEKKTASGYHGFEVNFSKYITLEDDLKRRDLTINSIAIDQNNKVIDPFNGQADLQNRI
HHHCCCCCCCCCCEEEECEEEEEECCCCHHCCEEEEEEEEECCCCEECCCCCCHHHHHHH
LRHTSIAFIEDPLRVVRLARFKAQLSNFNFSIAQEMLALIKELVKTGELNHLTRERLHIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FVKALNNPKIFFTTLKELEALKIIFPNISCILPLIPNKSFFENPIYKGSNINEKITLCLL
HHHHCCCCEEEEEEHHHHHHHEEECCCCEEEEEECCCCHHHCCCCCCCCCCCCCEEEEEE
KIPQQQLDDIRKELLLTNKHYKLLKASIAISKILEDRSITAEEIFQLIKNANIIRDKNLF
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECHHHH
AESLNLYKKYLKICDTITPHRNYQLLQTTINTIKNASIDSLTIKTIPKDKLRNTLKQLKL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA