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The map label for this gene is tpiA

Identifier: 134301294

GI number: 134301294

Start: 168323

End: 169069

Strand: Direct

Name: tpiA

Synonym: FTW_0156

Alternate gene names: 134301294

Gene position: 168323-169069 (Clockwise)

Preceding gene: 134301293

Following gene: 134301295

Centisome position: 8.87

GC content: 34.81

Gene sequence:

>747_bases
ATGGGTAACTGGAAAATGAATGGTAACTCTACAAGCATAAAAGAGCTCTGTAGTGGTATATCACAAGTGCAATATGATAC
TTCAAGAGTAGCTATTGCTGTTTTTCCATCAAGTGTTTATGTTAAAGAAGTAATCTCACAGCTGCCAGAGAAAGTAGGTG
TTGGTCTACAAAATATTACTTTTTATGATGATGGTGCTTATACTGGTGAGATATCTGCTAGGATGTTGGAAGATATTGGT
TGTGACTACTTACTAATTGGTCATTCTGAGAGAAGATCTCTATTTGCTGAGTCTGATGAAGATGTTTTTAAAAAGCTTAA
CAAGATTATAGATACTACTATAACGCCAGTAGTGTGTATTGGTGAATCACTAGATGATAGACAAAGTGGTAAGCTCAAAC
AAGTTTTAGCAACACAACTAAGCTTAATCTTAGAAAATTTATCTGTTGAGCAGTTAGCAAAAGTCGTAATTGCATATGAA
CCTGTCTGGGCAATAGGCACAGGAGTTGTGGCTTCACTAGAGCAGATTCAAGAAACACATCAATTTATTCGTTCATTGTT
AGCTAAAGTTGATGAAAGACTTGCTAAAAATATAAAAATAGTGTATGGTGGTAGCCTAAAAGCTGAAAATGCTAAAGATA
TATTAAGCTTACCAGATGTTGACGGTGGTTTAATTGGTGGCGCATCTTTGAAGGCTGCTGAATTTAACGAAATAATAAAT
CAAGCAAATAAGATATGTACGGAATAA

Upstream 100 bases:

>100_bases
TTATGGTTGAAGCAGATGACAAGAGTCTCGCTACAAACGAGGCTGAGTATTTGGTTGAAAAAGTAAAACAAAAATTGGTG
TAGATATGCAAAAATTAATA

Downstream 100 bases:

>100_bases
TTTTAACTATTGATATTATCGCAGCTATTGCGATTGTGGTACTAGTGTTGCTGCAGCAAGGTAAGGGCGCTAATATGGGC
GTTTCTTTTGGAGCAGGAGC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MGNWKMNGNSTSIKELCSGISQVQYDTSRVAIAVFPSSVYVKEVISQLPEKVGVGLQNITFYDDGAYTGEISARMLEDIG
CDYLLIGHSERRSLFAESDEDVFKKLNKIIDTTITPVVCIGESLDDRQSGKLKQVLATQLSLILENLSVEQLAKVVIAYE
PVWAIGTGVVASLEQIQETHQFIRSLLAKVDERLAKNIKIVYGGSLKAENAKDILSLPDVDGGLIGGASLKAAEFNEIIN
QANKICTE

Sequences:

>Translated_248_residues
MGNWKMNGNSTSIKELCSGISQVQYDTSRVAIAVFPSSVYVKEVISQLPEKVGVGLQNITFYDDGAYTGEISARMLEDIG
CDYLLIGHSERRSLFAESDEDVFKKLNKIIDTTITPVVCIGESLDDRQSGKLKQVLATQLSLILENLSVEQLAKVVIAYE
PVWAIGTGVVASLEQIQETHQFIRSLLAKVDERLAKNIKIVYGGSLKAENAKDILSLPDVDGGLIGGASLKAAEFNEIIN
QANKICTE
>Mature_247_residues
GNWKMNGNSTSIKELCSGISQVQYDTSRVAIAVFPSSVYVKEVISQLPEKVGVGLQNITFYDDGAYTGEISARMLEDIGC
DYLLIGHSERRSLFAESDEDVFKKLNKIIDTTITPVVCIGESLDDRQSGKLKQVLATQLSLILENLSVEQLAKVVIAYEP
VWAIGTGVVASLEQIQETHQFIRSLLAKVDERLAKNIKIVYGGSLKAENAKDILSLPDVDGGLIGGASLKAAEFNEIINQ
ANKICTE

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=242, Percent_Identity=42.1487603305785, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI4507645, Length=242, Percent_Identity=42.1487603305785, Blast_Score=189, Evalue=2e-48,
Organism=Escherichia coli, GI1790353, Length=243, Percent_Identity=42.798353909465, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17536593, Length=241, Percent_Identity=43.9834024896266, Blast_Score=188, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6320255, Length=244, Percent_Identity=39.7540983606557, Blast_Score=171, Evalue=1e-43,
Organism=Drosophila melanogaster, GI28572008, Length=240, Percent_Identity=41.6666666666667, Blast_Score=194, Evalue=4e-50,
Organism=Drosophila melanogaster, GI28572006, Length=240, Percent_Identity=41.6666666666667, Blast_Score=194, Evalue=4e-50,
Organism=Drosophila melanogaster, GI28572004, Length=240, Percent_Identity=41.6666666666667, Blast_Score=194, Evalue=5e-50,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_FRAT1 (Q14JZ0)

Other databases:

- EMBL:   AM286280
- RefSeq:   YP_666286.1
- ProteinModelPortal:   Q14JZ0
- SMR:   Q14JZ0
- STRING:   Q14JZ0
- GeneID:   4199859
- GenomeReviews:   AM286280_GR
- KEGG:   ftf:FTF0080
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   Q14JZ0
- ProtClustDB:   PRK14567
- BioCyc:   FTUL393115:FTF0080-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27042; Mature: 26911

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 93-93 ACT_SITE 165-165 BINDING 8-8 BINDING 10-10

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNWKMNGNSTSIKELCSGISQVQYDTSRVAIAVFPSSVYVKEVISQLPEKVGVGLQNIT
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEE
FYDDGAYTGEISARMLEDIGCDYLLIGHSERRSLFAESDEDVFKKLNKIIDTTITPVVCI
EECCCCEECHHHHHHHHHCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHCCHHEEE
GESLDDRQSGKLKQVLATQLSLILENLSVEQLAKVVIAYEPVWAIGTGVVASLEQIQETH
CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QFIRSLLAKVDERLAKNIKIVYGGSLKAENAKDILSLPDVDGGLIGGASLKAAEFNEIIN
HHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHCCCCCCCCEECCCCCCHHHHHHHHH
QANKICTE
HHHHHCCC
>Mature Secondary Structure 
GNWKMNGNSTSIKELCSGISQVQYDTSRVAIAVFPSSVYVKEVISQLPEKVGVGLQNIT
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEE
FYDDGAYTGEISARMLEDIGCDYLLIGHSERRSLFAESDEDVFKKLNKIIDTTITPVVCI
EECCCCEECHHHHHHHHHCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHCCHHEEE
GESLDDRQSGKLKQVLATQLSLILENLSVEQLAKVVIAYEPVWAIGTGVVASLEQIQETH
CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QFIRSLLAKVDERLAKNIKIVYGGSLKAENAKDILSLPDVDGGLIGGASLKAAEFNEIIN
HHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHCCCCCCCCEECCCCCCHHHHHHHHH
QANKICTE
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA