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The map label for this gene is cmlA [H]

Identifier: 134301220

GI number: 134301220

Start: 79893

End: 81074

Strand: Direct

Name: cmlA [H]

Synonym: FTW_0071

Alternate gene names: 134301220

Gene position: 79893-81074 (Clockwise)

Preceding gene: 134301218

Following gene: 134301222

Centisome position: 4.21

GC content: 36.21

Gene sequence:

>1182_bases
ATGTGGAAATATTCTCCATTAAAAACTATTCTTATCTTAGGACCTATGGTTTTTGCTTTTGCATTAGCTATGGATGTCTA
TATGCCTGTTCTACCTGATATGCGTGAGGCTCTTCATACAACTCAGCAGATGGTGCAAGTGACATTATCTTTATTTTTGG
TTGTTACAGGTGTCGGACAGCTTTTTTTAGGACCTCTGTCTGATCAGCTGGGTAGATTTAGAGTTATTCTATTATCAGCG
GTATTGTTTGTTATAGGTTCTGTGTTATGTGCTTTGTCTTCTAATATAGAATTTTTGATAGCCTCAAGAGTGGTGCAGGG
ATTAGGATGCTGTGGTTTGTCTGTTTGTGCATTTGCAATTATTCGTGATGCCTTTTCAGGTAAAACAAGTTCGATGATCT
ATAGTTTTATAAACGCAATTATATCCGTATCTCCTATTATCGGACCTTTAATAGGTGTGCAACTAGCTATTCATTTTCAT
TGGCAATCGGCATTTGTATTTCTTACTGGATTAGCAGTTGTAGCATTTTTGATAGTGGTGATATTTGTTAAAGAGAGCTT
ACCTGTTGAAAGGCGCAAAAAGATGTCTTGGAATGTATTTGCAAGATATCTATATGTGGCTAAGTCTTTACAGTTTTGGG
CTTTCTCATTAGCGGCTGTATCTGGAATGGCTTCATTTTTCATACTATTTTCGATGACGCCATATATTATCAATTATCTA
GGTTATCCAATATCAGAAATATATGTAGTATTTGGTTCAGCAGGATTAGCATTTTTGATAGGTTCTTTATTCGCGGGTGT
TATAGTTAATGCATTAGGTGTTTACAAAACGGCTTTGTTGGGGGTGGCATGTGTTTTTGCTGCAGGTATTTTATCCTTGA
GTATTTATGAAATATGGGGATTAAGCTTGTGGGGATTTTTTGCACCATGTTTTTTGGCAACATTTGGTTGCGCACTTACT
GTGGGCACTGGGGCAAGTGGTAGTATGGAACCTTTTTATGAAATAGCAGGTGTTGCTGCAGCATTATTTGGGACTATGGA
GTTTGCTATTAGTGGAATTATAGGAAGTATAGCAATGTTATTTCCAGCTACTAGTTCTCTGCCAATTGCAATTACAATGA
TTATTATGTCAATTTTATGTTTTGTGTTACTATTTTTGATAAAGGGCAAAACTAAACACTAG

Upstream 100 bases:

>100_bases
GCTGGTTTTATAAGTTTTAATACACTAAATCAATAACATATTTTTATTATTAATGGTATAATAATCGGCTATTCAATAAT
AAAATCAAGGAGTGTTTCTT

Downstream 100 bases:

>100_bases
ATAGAATATTAATAAATCTTCGAGTTTAAATAAAAGTATCCTAGATGTTTGGTGATTTATTTTTAATCATTAAAGCGAAC
TTCATATCTTCAGCTGTAAC

Product: major facilitator superfamily multidrug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MWKYSPLKTILILGPMVFAFALAMDVYMPVLPDMREALHTTQQMVQVTLSLFLVVTGVGQLFLGPLSDQLGRFRVILLSA
VLFVIGSVLCALSSNIEFLIASRVVQGLGCCGLSVCAFAIIRDAFSGKTSSMIYSFINAIISVSPIIGPLIGVQLAIHFH
WQSAFVFLTGLAVVAFLIVVIFVKESLPVERRKKMSWNVFARYLYVAKSLQFWAFSLAAVSGMASFFILFSMTPYIINYL
GYPISEIYVVFGSAGLAFLIGSLFAGVIVNALGVYKTALLGVACVFAAGILSLSIYEIWGLSLWGFFAPCFLATFGCALT
VGTGASGSMEPFYEIAGVAAALFGTMEFAISGIIGSIAMLFPATSSLPIAITMIIMSILCFVLLFLIKGKTKH

Sequences:

>Translated_393_residues
MWKYSPLKTILILGPMVFAFALAMDVYMPVLPDMREALHTTQQMVQVTLSLFLVVTGVGQLFLGPLSDQLGRFRVILLSA
VLFVIGSVLCALSSNIEFLIASRVVQGLGCCGLSVCAFAIIRDAFSGKTSSMIYSFINAIISVSPIIGPLIGVQLAIHFH
WQSAFVFLTGLAVVAFLIVVIFVKESLPVERRKKMSWNVFARYLYVAKSLQFWAFSLAAVSGMASFFILFSMTPYIINYL
GYPISEIYVVFGSAGLAFLIGSLFAGVIVNALGVYKTALLGVACVFAAGILSLSIYEIWGLSLWGFFAPCFLATFGCALT
VGTGASGSMEPFYEIAGVAAALFGTMEFAISGIIGSIAMLFPATSSLPIAITMIIMSILCFVLLFLIKGKTKH
>Mature_393_residues
MWKYSPLKTILILGPMVFAFALAMDVYMPVLPDMREALHTTQQMVQVTLSLFLVVTGVGQLFLGPLSDQLGRFRVILLSA
VLFVIGSVLCALSSNIEFLIASRVVQGLGCCGLSVCAFAIIRDAFSGKTSSMIYSFINAIISVSPIIGPLIGVQLAIHFH
WQSAFVFLTGLAVVAFLIVVIFVKESLPVERRKKMSWNVFARYLYVAKSLQFWAFSLAAVSGMASFFILFSMTPYIINYL
GYPISEIYVVFGSAGLAFLIGSLFAGVIVNALGVYKTALLGVACVFAAGILSLSIYEIWGLSLWGFFAPCFLATFGCALT
VGTGASGSMEPFYEIAGVAAALFGTMEFAISGIIGSIAMLFPATSSLPIAITMIIMSILCFVLLFLIKGKTKH

Specific function: Appears to provoke a reduction of the content of the major porins ompA and ompC [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=347, Percent_Identity=27.3775216138329, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI48994889, Length=346, Percent_Identity=28.6127167630058, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1788509, Length=376, Percent_Identity=26.063829787234, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790146, Length=345, Percent_Identity=25.5072463768116, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790794, Length=170, Percent_Identity=24.1176470588235, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787065, Length=202, Percent_Identity=26.2376237623762, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42354; Mature: 42354

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure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HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1648560; 1658833 [H]