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The map label for this gene is dnaN [H]

Identifier: 134301171

GI number: 134301171

Start: 1558

End: 2661

Strand: Direct

Name: dnaN [H]

Synonym: FTW_0002

Alternate gene names: 134301171

Gene position: 1558-2661 (Clockwise)

Preceding gene: 134301170

Following gene: 134301172

Centisome position: 0.08

GC content: 27.81

Gene sequence:

>1104_bases
ATGAATTTTGTACTAAATAGAGATGACTTACTAAAGCCTTTGCAATCTATGCTCTCAGTTGCAAATAGTAAGAGTACAAT
GCCTTTATTATCATGTATCTTATTTGATATTGATAATAATAATCTCAAAATTACGGCTTCGGATCTTGATACAGAGATAT
CATGCAATATAGCAGTTAGTTGTAACACAACTATTAAGTTAGCATTAAATGCTGACAAAATTTATAACATTGTCAGAAGC
TTAAATGAAAATTCAATGATTGATTTTAGAATTAATGAAAATAAGGTAACTATTGTTTCTAATAATAGTACTTTTAACCT
TATATCACTAAATGCTGACAACTATCCTCTTATTGATAGTAATATCAATGAGCAAGCAAGTTTTGATCTTTCTCAACAAG
ATTTTCATCATATTATTTCAAAAGTAGATTTCTCAATGGCTAATGATGATACTCGATATTTCTTAAATGGGATGTTTTGG
GAAATCAACGCAAATCTACTAAGAGCAGTATCTACAGATGGTCATAGAATGTCTATCACAGAGGCTATAATTGATAGTAA
AGTGTTAGATAGTGCTTCTCAGTCGATAATTCCAAAAAAAGCGATTTTAGAGCTTAAAAAGATAGTTGGCAAAACAGAAG
AAAATATCAAAATTTGTCTTGGCAAAAATTATCTAAAAGCGATTTTTGGTAATTATGCTTTTATATCAAAGCTTATAGAT
GGTCGCTATCCTGATTACCAAAAAGTAATCCCTAAAAATAATACAAAACTATTAGCAGTTGATAAGCAGTTTTTCAAAAA
TTCATTATTAAGAACATCAATACTTGCTAATGATAAATATAAAGGTGTTCGTCTTAACATATCTCAAAATCAATTACTTC
TATCAGCTAATAACCCTGATAATGAAAAGGCTGAAGATAAAATCGAAGTTCAATATAATGATCAACCAATGGAAATTTGT
TTTAATTACAAATATCTTTTGGATATTATAAATGTACTTAGTGAAGAAACTATGTCTATCTACCTTGATAATCCAAATAT
GAGTGCTTTAGTTAAAGATGAGAAAGATAATAGTTTGTTTATTATTATGCCAATGAAAATTTAA

Upstream 100 bases:

>100_bases
AAGACAAAGCAATACTTCAATATCGGATGATTATGAGTTGCTTTTAAATAAAATTTCTCGTTAAATAAAATTAGTAACTT
TATCAAAGGGGTTTTAAAAA

Downstream 100 bases:

>100_bases
GTAATAAGTAGTTTTAGGAAATAACTATTTTTATAAGCCTTTTGGAATGAATAATAAAGCAATAAAAAAAGGTATGCATA
AAAACATTATATAGAAAGCT

Product: DNA polymerase III, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MNFVLNRDDLLKPLQSMLSVANSKSTMPLLSCILFDIDNNNLKITASDLDTEISCNIAVSCNTTIKLALNADKIYNIVRS
LNENSMIDFRINENKVTIVSNNSTFNLISLNADNYPLIDSNINEQASFDLSQQDFHHIISKVDFSMANDDTRYFLNGMFW
EINANLLRAVSTDGHRMSITEAIIDSKVLDSASQSIIPKKAILELKKIVGKTEENIKICLGKNYLKAIFGNYAFISKLID
GRYPDYQKVIPKNNTKLLAVDKQFFKNSLLRTSILANDKYKGVRLNISQNQLLLSANNPDNEKAEDKIEVQYNDQPMEIC
FNYKYLLDIINVLSEETMSIYLDNPNMSALVKDEKDNSLFIIMPMKI

Sequences:

>Translated_367_residues
MNFVLNRDDLLKPLQSMLSVANSKSTMPLLSCILFDIDNNNLKITASDLDTEISCNIAVSCNTTIKLALNADKIYNIVRS
LNENSMIDFRINENKVTIVSNNSTFNLISLNADNYPLIDSNINEQASFDLSQQDFHHIISKVDFSMANDDTRYFLNGMFW
EINANLLRAVSTDGHRMSITEAIIDSKVLDSASQSIIPKKAILELKKIVGKTEENIKICLGKNYLKAIFGNYAFISKLID
GRYPDYQKVIPKNNTKLLAVDKQFFKNSLLRTSILANDKYKGVRLNISQNQLLLSANNPDNEKAEDKIEVQYNDQPMEIC
FNYKYLLDIINVLSEETMSIYLDNPNMSALVKDEKDNSLFIIMPMKI
>Mature_367_residues
MNFVLNRDDLLKPLQSMLSVANSKSTMPLLSCILFDIDNNNLKITASDLDTEISCNIAVSCNTTIKLALNADKIYNIVRS
LNENSMIDFRINENKVTIVSNNSTFNLISLNADNYPLIDSNINEQASFDLSQQDFHHIISKVDFSMANDDTRYFLNGMFW
EINANLLRAVSTDGHRMSITEAIIDSKVLDSASQSIIPKKAILELKKIVGKTEENIKICLGKNYLKAIFGNYAFISKLID
GRYPDYQKVIPKNNTKLLAVDKQFFKNSLLRTSILANDKYKGVRLNISQNQLLLSANNPDNEKAEDKIEVQYNDQPMEIC
FNYKYLLDIINVLSEETMSIYLDNPNMSALVKDEKDNSLFIIMPMKI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=369, Percent_Identity=34.1463414634146, Blast_Score=262, Evalue=3e-71,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41675; Mature: 41675

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFVLNRDDLLKPLQSMLSVANSKSTMPLLSCILFDIDNNNLKITASDLDTEISCNIAVS
CCEEECHHHHHHHHHHHHHHHCCCCCHHEEEEEEEEECCCCEEEEECCCCCEEEEEEEEE
CNTTIKLALNADKIYNIVRSLNENSMIDFRINENKVTIVSNNSTFNLISLNADNYPLIDS
ECCEEEEEECHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCEEEEEEECCCCCCEECC
NINEQASFDLSQQDFHHIISKVDFSMANDDTRYFLNGMFWEINANLLRAVSTDGHRMSIT
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCEEEEECCCCCEEEHH
EAIIDSKVLDSASQSIIPKKAILELKKIVGKTEENIKICLGKNYLKAIFGNYAFISKLID
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHCCHHHHHHHHC
GRYPDYQKVIPKNNTKLLAVDKQFFKNSLLRTSILANDKYKGVRLNISQNQLLLSANNPD
CCCCCHHHCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCC
NEKAEDKIEVQYNDQPMEICFNYKYLLDIINVLSEETMSIYLDNPNMSALVKDEKDNSLF
CCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCEEE
IIMPMKI
EEEECCC
>Mature Secondary Structure
MNFVLNRDDLLKPLQSMLSVANSKSTMPLLSCILFDIDNNNLKITASDLDTEISCNIAVS
CCEEECHHHHHHHHHHHHHHHCCCCCHHEEEEEEEEECCCCEEEEECCCCCEEEEEEEEE
CNTTIKLALNADKIYNIVRSLNENSMIDFRINENKVTIVSNNSTFNLISLNADNYPLIDS
ECCEEEEEECHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCEEEEEEECCCCCCEECC
NINEQASFDLSQQDFHHIISKVDFSMANDDTRYFLNGMFWEINANLLRAVSTDGHRMSIT
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCEEEEECCCCCEEEHH
EAIIDSKVLDSASQSIIPKKAILELKKIVGKTEENIKICLGKNYLKAIFGNYAFISKLID
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHCCHHHHHHHHC
GRYPDYQKVIPKNNTKLLAVDKQFFKNSLLRTSILANDKYKGVRLNISQNQLLLSANNPD
CCCCCHHHCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCC
NEKAEDKIEVQYNDQPMEICFNYKYLLDIINVLSEETMSIYLDNPNMSALVKDEKDNSLF
CCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCEEE
IIMPMKI
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]