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The map label for this gene is pleD [H]

Identifier: 134300533

GI number: 134300533

Start: 2927802

End: 2929055

Strand: Reverse

Name: pleD [H]

Synonym: Dred_2700

Alternate gene names: 134300533

Gene position: 2929055-2927802 (Counterclockwise)

Preceding gene: 134300534

Following gene: 134300531

Centisome position: 81.18

GC content: 40.83

Gene sequence:

>1254_bases
ATGGTAAATATACGAATTACCTCACCTCTAAAAATCTTTTTGGTATCCTTTTTAGCCATGACCATTGCTATGGCTACACT
GGCCTATGCCACCTACCAGGCCTGCTACGACATTTTGGCCAAGGACCTGGGCCTACGGGCCCAATTTTCGGCTCAAGTTG
CGGCAAGTCTTATTGAAGTGGATCAGGAACTCGTAAATCAAATGAAGCAATTGGATATCCTAAGCTCCCGGAATCACGTT
GCAGCAATGGAATTCAAGCGAAAAATGGCACCCCTGTTAAAGCACAGTGACATTAAATATATTTATGTCGAGACTAAGTT
GTCAGGCAGTGAAATACGGTATTTTGTAAGCCCTGAGGAGGAAAAACTATATGCACAGCCACCGGGAACACCCATGGAGT
ATTTTTATGTCCTGACCAGTGAAGATGAAAGTGGCTATCGAAATCGAGATCGCTATGATGTTGCCAATCCTTTAAGAGAA
AGGGCCTATGTAGAAAGGGTACCTACCTATGGCCTGGACACTAACAACAGATGGGGTAGTGTGATAACTGGTTATCAGCC
TCTTTTTGACCATAATAATCAATTTATTGGTTTACTGGGTGTTGATATTGCATTGCATCAATTTGACCGTTCAGTCTGGT
ATGTAAAAAACATTGTAATTATGAGCTTTGGCATTATCGTGTTGTTAGGGGGTATCATTCTCTTTTTTGCCTCAAAGATA
CTAAGCAAGCCCCTATACATGGATGAATTGACAAAATTGTTTAACCGGGAATACATGAAAATTCGTTTAGAGAGCGAAAT
AAGACGGGCCAGGCGGAATAAGCTTCCTCTTAGCTTTCTCATGCTGGATCTGGACTATTTTAAAAGTATTAATGACTGTT
TTGGCCATCAAACAGGTGATTTAATATTGAAAAAAATCTCTGAATTAATCTGTGTATCCCTAAGGGAAGAAGACCTTGCC
TTCCGATATGGTGGTGAAGAAATAGCCATTTTGTTACCGGAAACCGGACTTTATGAAGCAAAGATTGTGGCAGAAAGGTT
ACGTCAGGTCATTGAAACAAACAGTTTGTATGTTAATCATTATGAGGAGCCTATTCATGTAACCATCAGTATTGGCCTTG
CTGAACTGGCAGCAAAGGATACACAGGAAACTTTGATTAAAAAAGCAGATGATGCGTTGTACCTGGCGAAGAAGAAGGGT
CGCAACTGCTATCAATGCATTGAGGAATCTGGAAAAAACCTTTCTCCGGAATAA

Upstream 100 bases:

>100_bases
TGGCGAAGCCAAGTTTTTCTAATAAACCCAAGAGGAATTTTATTCCTATATGTTTAAATTCTTTAGTTGTCCAGAAAAAG
AAAAATGAGTAGGTAAAAAG

Downstream 100 bases:

>100_bases
TGCTCCGGAGTTTTTTATTTTTACTTTTTGTCGGGTATCATAGGCATAAGAAGTTGGCTAATACCCTATGCTATGGTAAA
GTTTATGTGCAGTTAAAATA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MVNIRITSPLKIFLVSFLAMTIAMATLAYATYQACYDILAKDLGLRAQFSAQVAASLIEVDQELVNQMKQLDILSSRNHV
AAMEFKRKMAPLLKHSDIKYIYVETKLSGSEIRYFVSPEEEKLYAQPPGTPMEYFYVLTSEDESGYRNRDRYDVANPLRE
RAYVERVPTYGLDTNNRWGSVITGYQPLFDHNNQFIGLLGVDIALHQFDRSVWYVKNIVIMSFGIIVLLGGIILFFASKI
LSKPLYMDELTKLFNREYMKIRLESEIRRARRNKLPLSFLMLDLDYFKSINDCFGHQTGDLILKKISELICVSLREEDLA
FRYGGEEIAILLPETGLYEAKIVAERLRQVIETNSLYVNHYEEPIHVTISIGLAELAAKDTQETLIKKADDALYLAKKKG
RNCYQCIEESGKNLSPE

Sequences:

>Translated_417_residues
MVNIRITSPLKIFLVSFLAMTIAMATLAYATYQACYDILAKDLGLRAQFSAQVAASLIEVDQELVNQMKQLDILSSRNHV
AAMEFKRKMAPLLKHSDIKYIYVETKLSGSEIRYFVSPEEEKLYAQPPGTPMEYFYVLTSEDESGYRNRDRYDVANPLRE
RAYVERVPTYGLDTNNRWGSVITGYQPLFDHNNQFIGLLGVDIALHQFDRSVWYVKNIVIMSFGIIVLLGGIILFFASKI
LSKPLYMDELTKLFNREYMKIRLESEIRRARRNKLPLSFLMLDLDYFKSINDCFGHQTGDLILKKISELICVSLREEDLA
FRYGGEEIAILLPETGLYEAKIVAERLRQVIETNSLYVNHYEEPIHVTISIGLAELAAKDTQETLIKKADDALYLAKKKG
RNCYQCIEESGKNLSPE
>Mature_417_residues
MVNIRITSPLKIFLVSFLAMTIAMATLAYATYQACYDILAKDLGLRAQFSAQVAASLIEVDQELVNQMKQLDILSSRNHV
AAMEFKRKMAPLLKHSDIKYIYVETKLSGSEIRYFVSPEEEKLYAQPPGTPMEYFYVLTSEDESGYRNRDRYDVANPLRE
RAYVERVPTYGLDTNNRWGSVITGYQPLFDHNNQFIGLLGVDIALHQFDRSVWYVKNIVIMSFGIIVLLGGIILFFASKI
LSKPLYMDELTKLFNREYMKIRLESEIRRARRNKLPLSFLMLDLDYFKSINDCFGHQTGDLILKKISELICVSLREEDLA
FRYGGEEIAILLPETGLYEAKIVAERLRQVIETNSLYVNHYEEPIHVTISIGLAELAAKDTQETLIKKADDALYLAKKKG
RNCYQCIEESGKNLSPE

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=164, Percent_Identity=42.6829268292683, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1787262, Length=205, Percent_Identity=34.1463414634146, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=37.888198757764, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786584, Length=156, Percent_Identity=32.6923076923077, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1788085, Length=199, Percent_Identity=32.1608040201005, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=30.6748466257669, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081881, Length=125, Percent_Identity=40, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787802, Length=160, Percent_Identity=31.875, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081974, Length=202, Percent_Identity=27.2277227722772, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788381, Length=157, Percent_Identity=30.5732484076433, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787541, Length=161, Percent_Identity=30.4347826086957, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 47848; Mature: 47848

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNIRITSPLKIFLVSFLAMTIAMATLAYATYQACYDILAKDLGLRAQFSAQVAASLIEV
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
DQELVNQMKQLDILSSRNHVAAMEFKRKMAPLLKHSDIKYIYVETKLSGSEIRYFVSPEE
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEECCCC
EKLYAQPPGTPMEYFYVLTSEDESGYRNRDRYDVANPLRERAYVERVPTYGLDTNNRWGS
CCEEECCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VITGYQPLFDHNNQFIGLLGVDIALHQFDRSVWYVKNIVIMSFGIIVLLGGIILFFASKI
EECCCCHHHCCCCCEEEEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKPLYMDELTKLFNREYMKIRLESEIRRARRNKLPLSFLMLDLDYFKSINDCFGHQTGD
HCCCCCHHHHHHHHCCHHEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHH
LILKKISELICVSLREEDLAFRYGGEEIAILLPETGLYEAKIVAERLRQVIETNSLYVNH
HHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEE
YEEPIHVTISIGLAELAAKDTQETLIKKADDALYLAKKKGRNCYQCIEESGKNLSPE
CCCCEEEEEEECHHHHHHCCHHHHHHHHCCCCEEEHHHCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVNIRITSPLKIFLVSFLAMTIAMATLAYATYQACYDILAKDLGLRAQFSAQVAASLIEV
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
DQELVNQMKQLDILSSRNHVAAMEFKRKMAPLLKHSDIKYIYVETKLSGSEIRYFVSPEE
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEECCCC
EKLYAQPPGTPMEYFYVLTSEDESGYRNRDRYDVANPLRERAYVERVPTYGLDTNNRWGS
CCEEECCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VITGYQPLFDHNNQFIGLLGVDIALHQFDRSVWYVKNIVIMSFGIIVLLGGIILFFASKI
EECCCCHHHCCCCCEEEEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKPLYMDELTKLFNREYMKIRLESEIRRARRNKLPLSFLMLDLDYFKSINDCFGHQTGD
HCCCCCHHHHHHHHCCHHEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHH
LILKKISELICVSLREEDLAFRYGGEEIAILLPETGLYEAKIVAERLRQVIETNSLYVNH
HHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEE
YEEPIHVTISIGLAELAAKDTQETLIKKADDALYLAKKKGRNCYQCIEESGKNLSPE
CCCCEEEEEEECHHHHHHCCHHHHHHHHCCCCEEEHHHCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA