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The map label for this gene is cwlC [H]

Identifier: 134300428

GI number: 134300428

Start: 2814935

End: 2815501

Strand: Reverse

Name: cwlC [H]

Synonym: Dred_2589

Alternate gene names: 134300428

Gene position: 2815501-2814935 (Counterclockwise)

Preceding gene: 134300429

Following gene: 134300427

Centisome position: 78.03

GC content: 45.68

Gene sequence:

>567_bases
ATGAAACGAATTGTCCTTGATCCGGGGCATGGGGGAGCAGATCCAGGAGCAGTTGGAAACGGATTGTTAGAAAAACAAGT
TACGTGGATGCTGGCTAACAAGGTCAAAGAAAAGCTAAAGCGCATGAAAGCTGAAGTAATTATCGTTCAGCCAAGTTGCG
GGAATCCCAGAAGCACAAAGGATGATGAACTGTACCTGCCGCCCAGGGATGCTAATCGGTTGGGGGCCGATTTTTATCTT
TCCATTCATGTTAACGCTGGAGGCGGTACTGGCTTTGAATCCTTTGTGCATCAAAACAGCCAGGGCAAGGATACTGATAA
GTTGAGGAACGTTCTTCACCGTCAGGTCATGGCCTACTTAGCTAAGTATGGGATAGTTGACAGGGGCAAGAAGTACGCAA
ACTTTGCGGTATTACGGTTAACCAATATGCCAGCGGTATTGATAGAGTGTTTGTTTATTGATAACGCCAAAGATGCGGTA
CTATTAAAAGACCAATCTTTTATTGACGGGTTAGCCAATGAAATCGCCTATGGGTTAATTGTTGCACTAGGGCTGCAGAG
GGGGTAA

Upstream 100 bases:

>100_bases
CTAGTTTATGTGGCTCCGATTGTGGGTGCTATTATCACATATTACTTTGCCACCGAAGGCGCTACATTGTATTTCAACCG
AAAGAAAGGAGAAGGAGAAA

Downstream 100 bases:

>100_bases
CGATATGGATATTCGTCCATTTGTTGAAAGGTTAAAATCGAGGAAGTTTATCATGGCACTGGTAGCGGCTGCAGTTGCCG
GTACCAAGGTTTATTATCCG

Product: cell wall hydrolase/autolysin

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MKRIVLDPGHGGADPGAVGNGLLEKQVTWMLANKVKEKLKRMKAEVIIVQPSCGNPRSTKDDELYLPPRDANRLGADFYL
SIHVNAGGGTGFESFVHQNSQGKDTDKLRNVLHRQVMAYLAKYGIVDRGKKYANFAVLRLTNMPAVLIECLFIDNAKDAV
LLKDQSFIDGLANEIAYGLIVALGLQRG

Sequences:

>Translated_188_residues
MKRIVLDPGHGGADPGAVGNGLLEKQVTWMLANKVKEKLKRMKAEVIIVQPSCGNPRSTKDDELYLPPRDANRLGADFYL
SIHVNAGGGTGFESFVHQNSQGKDTDKLRNVLHRQVMAYLAKYGIVDRGKKYANFAVLRLTNMPAVLIECLFIDNAKDAV
LLKDQSFIDGLANEIAYGLIVALGLQRG
>Mature_188_residues
MKRIVLDPGHGGADPGAVGNGLLEKQVTWMLANKVKEKLKRMKAEVIIVQPSCGNPRSTKDDELYLPPRDANRLGADFYL
SIHVNAGGGTGFESFVHQNSQGKDTDKLRNVLHRQVMAYLAKYGIVDRGKKYANFAVLRLTNMPAVLIECLFIDNAKDAV
LLKDQSFIDGLANEIAYGLIVALGLQRG

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. CwlC is able to hydrolyze typ

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall. Note=Is associated with the mother cell wall of sporulating cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR007730 [H]

Pfam domain/function: PF01520 Amidase_3; PF05036 SPOR [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20622; Mature: 20622

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIVLDPGHGGADPGAVGNGLLEKQVTWMLANKVKEKLKRMKAEVIIVQPSCGNPRSTK
CCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC
DDELYLPPRDANRLGADFYLSIHVNAGGGTGFESFVHQNSQGKDTDKLRNVLHRQVMAYL
CCEEEECCCCCCCCCCEEEEEEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
AKYGIVDRGKKYANFAVLRLTNMPAVLIECLFIDNAKDAVLLKDQSFIDGLANEIAYGLI
HHCCCHHCCCHHHCEEEEEECCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHH
VALGLQRG
HHHHCCCC
>Mature Secondary Structure
MKRIVLDPGHGGADPGAVGNGLLEKQVTWMLANKVKEKLKRMKAEVIIVQPSCGNPRSTK
CCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC
DDELYLPPRDANRLGADFYLSIHVNAGGGTGFESFVHQNSQGKDTDKLRNVLHRQVMAYL
CCEEEECCCCCCCCCCEEEEEEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
AKYGIVDRGKKYANFAVLRLTNMPAVLIECLFIDNAKDAVLLKDQSFIDGLANEIAYGLI
HHCCCHHCCCHHHCEEEEEECCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHH
VALGLQRG
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407798; 9384377 [H]