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The map label for this gene is ppsA [H]

Identifier: 134300305

GI number: 134300305

Start: 2685107

End: 2687455

Strand: Reverse

Name: ppsA [H]

Synonym: Dred_2466

Alternate gene names: 134300305

Gene position: 2687455-2685107 (Counterclockwise)

Preceding gene: 134300306

Following gene: 134300304

Centisome position: 74.48

GC content: 42.61

Gene sequence:

>2349_bases
TTGGGGTACCAGTTTATTAAATGGTTTAATGAACTAACCAAAGAAGATGTCGCTATGGTTGGTGGAAAAGGGGCTAATTT
AGGTGAACTTACAAGGGCAGGCATTCGAGTACCGCCCGGTTTTTGTGTAACGGCCGATGCATATCAATATTTTGTTAAAT
CCACTGGTTTACAGGTAAAAATTCGTGCGTTATTGGCAGAAACAGACTTAAGTGATGTTCAACAGTTAGATGAGAATACA
GCTAAAATACGCCAAATGATTGTTTTGACTTCTATGCCCACGGAAATTAAGAATGAAATCATCTCCGCTTATGGCTATTT
GACCGGTTCCGGCAATGCCCCACGTGTAGCTGTGCGCAGTTCTGCTACTGCAGAAGATTTACCAGAAGCGTCTTTTGCCG
GTCAACAAGATACCTATTTAAATATATTAGGGGCTAAAGAAGTAGTTTCTCATGTCCAAAAATGTTGGGCATCACTATGG
ACAGCCCGAGCAACCTACTACCGTCAAACTCAAAAGTTTGATCATTTTGATGTTTACCTGTCGGCTGTTGTGCAAAAACT
GGTTTCTGCCGCTAAATCCGGAGTAGCATTCACCGCAAACCCGGTAACCGGAACCAGAGATGAAATTATGATTAACGCTA
GTTGGGGGCTGGGAGAAGCTGTTGTTGCTGGTATAGTAACCCCTGACGATTATATTATACAAAAAGATAAATGGGAAATA
AAAGAAAAAGAAGTTGCTGAAAAAAAGATCATGGTAATAAGGGACCCAGCCAAAGAAGTAGGAACCATCACGGTACCGGT
CAGTGAACATTTAGGAAATGATTATGTTACCAAACAATGCCTGTCCGATGATGAAATTATCAACCTGTCCAAAGAGTGTA
CCCGTATAGAAAAGCATTACGGTTCTCCACAGGATATAGAGTGGGCTTACGATAATGATGACGGGCAGTTCTATCTGTTA
CAGGCTCGCCCGATAACCACTTTAGGAGAGGAGAGAAAAGATAATATGAGCGAGGTAAAAAAAGAAGACCTGGAAGTATT
AGTCAAAGGCATGGCTGCATCTCCTGGCATAGCTTCCGGAAAAGTAACGATCATTCGTAATGATGATATCTCCGGTGTTA
AAGAAGGGGATATTCTGGTGACGATTATGACGAACCCTGATATGGTTCCAGCCATGAGTAAAGCTGCTGCTATCGTGACA
GACGAAGGGGGTCGAACCTGTCACGCAGCTATTGTATCCCGTGAGTTGGGAGTGCCTTGTATTGTTGGCTGCAAAAATGC
TACAGATGTTCTTAAAGAAGGCCAGGAAGTTACCGTTGATGCTACAAGGGGTGTCGTGTACAAAGGTAAAGTAGATCTCG
GGCAGAATAAGACAGAAACAGCTAACAGTGCTGCTACCTTCCCTCCGGAAATCATGCGGGAAATATTCCCGGTTACTGCA
ACCAAAGTATATATGAATCTGGGAGAGCCGTCTTTGGTAGAGAAGTATAAGAATTTACCCTTCGATGGTATTGGTCTAAT
GAGGGTGGAATTTATTTTTACGAATATGATTAAGGCTCACCCCATGTATCTGATTAAAACTGGCCAGCAACAGTTTATGA
TTGATAAACTGGCCGAAGGATTAACTAGCGTGGCCCAGGCCATATACCCACGTCCAGTTGTGGTAAGAATGAGTGATTTT
AGAACAAATGAATTTCGTGGTCTAAAGGGCGGCGAAGAAGTTGAACCTATTGAGAATAATCCGATGATTGGTTGGCGCGG
AGTTTCCCGTTATATTTCTGAAGAATACTTAGAAGGTTTTCGTGCAGAATGCAAGGCCATGCGTAAAGCACGGGAAGAGT
ATGGCTTAACCAATATTTGGGCGATGTTACCCTTTGTCCGTACCACCTGGGAATTAAAACAGGTTAAGGCTATCATGGAG
GAAGAGGGCCTCAAACAAGGCCTGAATTTTAAACTTTGGATTATGGCGGAAGTGCCTTCGGTAATATTTATGGCTGATGA
GTTTAGTCAATTAGTTGATGGGTTTAGTATTGGTAGCAACGATTTAACCCAATTAATACTTGGTGCAGACCGTGACTCTG
GGGTTCTGGATAGCATGGGCTATTTCGATGAGCGTGACCCTGCCATTAAAAGAGCCATTGCCCATTTAATAGAAACCGCT
CATAAATACGGCAAAACGGTTTCCATTTGCGGACAGGGTCCATCAATTTATCCGGAATTCACTGAATTTTTGGTTAAAAC
AGGTATTGACAGTGTTAGTGTAAACCCAGATACGGTCATTCAAACCCGCCGTTTGGTGGCTTCTGTAGAACAGAAAGTAA
TGTTGGAATTACTTAGGGGCAAAAAATAA

Upstream 100 bases:

>100_bases
AATCTCATTATGAAATTTAGCCCTTACTTATATTCCGGCCCAAAAGGTCTTGCCGGATATGTAATAAAATGTTCTTCAAT
AAAGAGAGGAGTTGGTAGCA

Downstream 100 bases:

>100_bases
GGCATTTGTTTTATAAAATACGATTAACTCACGGATTCAGGAACTAGATAGATAAGTGATTCTCAGGGCAGCCTTGCTGC
CCTGATTTGTTTTTCAAAAA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 782; Mature: 781

Protein sequence:

>782_residues
MGYQFIKWFNELTKEDVAMVGGKGANLGELTRAGIRVPPGFCVTADAYQYFVKSTGLQVKIRALLAETDLSDVQQLDENT
AKIRQMIVLTSMPTEIKNEIISAYGYLTGSGNAPRVAVRSSATAEDLPEASFAGQQDTYLNILGAKEVVSHVQKCWASLW
TARATYYRQTQKFDHFDVYLSAVVQKLVSAAKSGVAFTANPVTGTRDEIMINASWGLGEAVVAGIVTPDDYIIQKDKWEI
KEKEVAEKKIMVIRDPAKEVGTITVPVSEHLGNDYVTKQCLSDDEIINLSKECTRIEKHYGSPQDIEWAYDNDDGQFYLL
QARPITTLGEERKDNMSEVKKEDLEVLVKGMAASPGIASGKVTIIRNDDISGVKEGDILVTIMTNPDMVPAMSKAAAIVT
DEGGRTCHAAIVSRELGVPCIVGCKNATDVLKEGQEVTVDATRGVVYKGKVDLGQNKTETANSAATFPPEIMREIFPVTA
TKVYMNLGEPSLVEKYKNLPFDGIGLMRVEFIFTNMIKAHPMYLIKTGQQQFMIDKLAEGLTSVAQAIYPRPVVVRMSDF
RTNEFRGLKGGEEVEPIENNPMIGWRGVSRYISEEYLEGFRAECKAMRKAREEYGLTNIWAMLPFVRTTWELKQVKAIME
EEGLKQGLNFKLWIMAEVPSVIFMADEFSQLVDGFSIGSNDLTQLILGADRDSGVLDSMGYFDERDPAIKRAIAHLIETA
HKYGKTVSICGQGPSIYPEFTEFLVKTGIDSVSVNPDTVIQTRRLVASVEQKVMLELLRGKK

Sequences:

>Translated_782_residues
MGYQFIKWFNELTKEDVAMVGGKGANLGELTRAGIRVPPGFCVTADAYQYFVKSTGLQVKIRALLAETDLSDVQQLDENT
AKIRQMIVLTSMPTEIKNEIISAYGYLTGSGNAPRVAVRSSATAEDLPEASFAGQQDTYLNILGAKEVVSHVQKCWASLW
TARATYYRQTQKFDHFDVYLSAVVQKLVSAAKSGVAFTANPVTGTRDEIMINASWGLGEAVVAGIVTPDDYIIQKDKWEI
KEKEVAEKKIMVIRDPAKEVGTITVPVSEHLGNDYVTKQCLSDDEIINLSKECTRIEKHYGSPQDIEWAYDNDDGQFYLL
QARPITTLGEERKDNMSEVKKEDLEVLVKGMAASPGIASGKVTIIRNDDISGVKEGDILVTIMTNPDMVPAMSKAAAIVT
DEGGRTCHAAIVSRELGVPCIVGCKNATDVLKEGQEVTVDATRGVVYKGKVDLGQNKTETANSAATFPPEIMREIFPVTA
TKVYMNLGEPSLVEKYKNLPFDGIGLMRVEFIFTNMIKAHPMYLIKTGQQQFMIDKLAEGLTSVAQAIYPRPVVVRMSDF
RTNEFRGLKGGEEVEPIENNPMIGWRGVSRYISEEYLEGFRAECKAMRKAREEYGLTNIWAMLPFVRTTWELKQVKAIME
EEGLKQGLNFKLWIMAEVPSVIFMADEFSQLVDGFSIGSNDLTQLILGADRDSGVLDSMGYFDERDPAIKRAIAHLIETA
HKYGKTVSICGQGPSIYPEFTEFLVKTGIDSVSVNPDTVIQTRRLVASVEQKVMLELLRGKK
>Mature_781_residues
GYQFIKWFNELTKEDVAMVGGKGANLGELTRAGIRVPPGFCVTADAYQYFVKSTGLQVKIRALLAETDLSDVQQLDENTA
KIRQMIVLTSMPTEIKNEIISAYGYLTGSGNAPRVAVRSSATAEDLPEASFAGQQDTYLNILGAKEVVSHVQKCWASLWT
ARATYYRQTQKFDHFDVYLSAVVQKLVSAAKSGVAFTANPVTGTRDEIMINASWGLGEAVVAGIVTPDDYIIQKDKWEIK
EKEVAEKKIMVIRDPAKEVGTITVPVSEHLGNDYVTKQCLSDDEIINLSKECTRIEKHYGSPQDIEWAYDNDDGQFYLLQ
ARPITTLGEERKDNMSEVKKEDLEVLVKGMAASPGIASGKVTIIRNDDISGVKEGDILVTIMTNPDMVPAMSKAAAIVTD
EGGRTCHAAIVSRELGVPCIVGCKNATDVLKEGQEVTVDATRGVVYKGKVDLGQNKTETANSAATFPPEIMREIFPVTAT
KVYMNLGEPSLVEKYKNLPFDGIGLMRVEFIFTNMIKAHPMYLIKTGQQQFMIDKLAEGLTSVAQAIYPRPVVVRMSDFR
TNEFRGLKGGEEVEPIENNPMIGWRGVSRYISEEYLEGFRAECKAMRKAREEYGLTNIWAMLPFVRTTWELKQVKAIMEE
EGLKQGLNFKLWIMAEVPSVIFMADEFSQLVDGFSIGSNDLTQLILGADRDSGVLDSMGYFDERDPAIKRAIAHLIETAH
KYGKTVSICGQGPSIYPEFTEFLVKTGIDSVSVNPDTVIQTRRLVASVEQKVMLELLRGKK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=800, Percent_Identity=41.375, Blast_Score=593, Evalue=1e-170,
Organism=Escherichia coli, GI1788756, Length=415, Percent_Identity=26.2650602409639, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI1789193, Length=396, Percent_Identity=26.5151515151515, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1788726, Length=373, Percent_Identity=27.3458445040214, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI48994992, Length=368, Percent_Identity=26.3586956521739, Blast_Score=101, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17564524, Length=333, Percent_Identity=29.1291291291291, Blast_Score=114, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 86644; Mature: 86513

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2 ; PS01319 RBFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYQFIKWFNELTKEDVAMVGGKGANLGELTRAGIRVPPGFCVTADAYQYFVKSTGLQVK
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCCEEE
IRALLAETDLSDVQQLDENTAKIRQMIVLTSMPTEIKNEIISAYGYLTGSGNAPRVAVRS
EEHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCEEECCCCCCEEEEEC
SATAEDLPEASFAGQQDTYLNILGAKEVVSHVQKCWASLWTARATYYRQTQKFDHFDVYL
CCCHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SAVVQKLVSAAKSGVAFTANPVTGTRDEIMINASWGLGEAVVAGIVTPDDYIIQKDKWEI
HHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCHHHHEEECCCCCEEEECCCCCC
KEKEVAEKKIMVIRDPAKEVGTITVPVSEHLGNDYVTKQCLSDDEIINLSKECTRIEKHY
HHHHHHHHEEEEEECCHHHCCEEEEEHHHHCCCHHHHHHHCCCCHHCCCHHHHHHHHHHC
GSPQDIEWAYDNDDGQFYLLQARPITTLGEERKDNMSEVKKEDLEVLVKGMAASPGIASG
CCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCC
KVTIIRNDDISGVKEGDILVTIMTNPDMVPAMSKAAAIVTDEGGRTCHAAIVSRELGVPC
EEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHCEEEEECCCCCEEHHHHHHHHCCCCE
IVGCKNATDVLKEGQEVTVDATRGVVYKGKVDLGQNKTETANSAATFPPEIMREIFPVTA
EEECCCHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCHHH
TKVYMNLGEPSLVEKYKNLPFDGIGLMRVEFIFTNMIKAHPMYLIKTGQQQFMIDKLAEG
EEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHH
LTSVAQAIYPRPVVVRMSDFRTNEFRGLKGGEEVEPIENNPMIGWRGVSRYISEEYLEGF
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
RAECKAMRKAREEYGLTNIWAMLPFVRTTWELKQVKAIMEEEGLKQGLNFKLWIMAEVPS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
VIFMADEFSQLVDGFSIGSNDLTQLILGADRDSGVLDSMGYFDERDPAIKRAIAHLIETA
CEEEHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHH
HKYGKTVSICGQGPSIYPEFTEFLVKTGIDSVSVNPDTVIQTRRLVASVEQKVMLELLRG
HHHCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
KK
CC
>Mature Secondary Structure 
GYQFIKWFNELTKEDVAMVGGKGANLGELTRAGIRVPPGFCVTADAYQYFVKSTGLQVK
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCCEEE
IRALLAETDLSDVQQLDENTAKIRQMIVLTSMPTEIKNEIISAYGYLTGSGNAPRVAVRS
EEHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCEEECCCCCCEEEEEC
SATAEDLPEASFAGQQDTYLNILGAKEVVSHVQKCWASLWTARATYYRQTQKFDHFDVYL
CCCHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SAVVQKLVSAAKSGVAFTANPVTGTRDEIMINASWGLGEAVVAGIVTPDDYIIQKDKWEI
HHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCHHHHEEECCCCCEEEECCCCCC
KEKEVAEKKIMVIRDPAKEVGTITVPVSEHLGNDYVTKQCLSDDEIINLSKECTRIEKHY
HHHHHHHHEEEEEECCHHHCCEEEEEHHHHCCCHHHHHHHCCCCHHCCCHHHHHHHHHHC
GSPQDIEWAYDNDDGQFYLLQARPITTLGEERKDNMSEVKKEDLEVLVKGMAASPGIASG
CCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCC
KVTIIRNDDISGVKEGDILVTIMTNPDMVPAMSKAAAIVTDEGGRTCHAAIVSRELGVPC
EEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHCEEEEECCCCCEEHHHHHHHHCCCCE
IVGCKNATDVLKEGQEVTVDATRGVVYKGKVDLGQNKTETANSAATFPPEIMREIFPVTA
EEECCCHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCHHH
TKVYMNLGEPSLVEKYKNLPFDGIGLMRVEFIFTNMIKAHPMYLIKTGQQQFMIDKLAEG
EEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHH
LTSVAQAIYPRPVVVRMSDFRTNEFRGLKGGEEVEPIENNPMIGWRGVSRYISEEYLEGF
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
RAECKAMRKAREEYGLTNIWAMLPFVRTTWELKQVKAIMEEEGLKQGLNFKLWIMAEVPS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
VIFMADEFSQLVDGFSIGSNDLTQLILGADRDSGVLDSMGYFDERDPAIKRAIAHLIETA
CEEEHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHH
HKYGKTVSICGQGPSIYPEFTEFLVKTGIDSVSVNPDTVIQTRRLVASVEQKVMLELLRG
HHHCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
KK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7805870 [H]