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The map label for this gene is purF [H]

Identifier: 134300204

GI number: 134300204

Start: 2588904

End: 2590328

Strand: Reverse

Name: purF [H]

Synonym: Dred_2365

Alternate gene names: 134300204

Gene position: 2590328-2588904 (Counterclockwise)

Preceding gene: 134300205

Following gene: 134300203

Centisome position: 71.79

GC content: 51.02

Gene sequence:

>1425_bases
ATGTTTAATGAAATAGGTACGATCCTTTGGTCCGATAAGCCAGTGGAAGAATGTGGTGTGTTTGGCATTTTTGCTCCTGG
GTTGGATGTGGCTCGTTTAACTTACTACGGGCTTCACGCCCTGCAGCATAGGGGACAGGAAAGTGCCGGTATTGCGGTGG
CCGATGGAAGTCAGATTGAACTTCAAAAGGGAATGGGGCTGGTACCGGAAGTTTTTTCGGGGCATAGTCTGGATAAATTC
TCGGGCTTTGCTGCCATTGGACATGTACGCTATTCCACCACTGGCGCCAGTTCTCCCCTTAACGCCCAGCCCCTGGTGTT
TCGTTACGCCAAGGGGATGTTGGGGTTGGCGCATAATGGAAACCTCACCAATGTGGCGGACCTGCGAGCTCAATTGGCCT
CCACTGGTTCGGTCTTTCAATCCACCACTGACAGTGAGGTGCTGGTGAACTTGATTGCCCGGTATAATGCTAACAGCGTG
GAAGAAGCTCTGATGAAATGCATGATTGATGTTAAAGGGGCCTATGCATTGCTAATTATCACCGAGGATACTTTATATGC
TGCACGGGATCCCTACGGTCTCAGGCCCCTTTGTTTGGGTCGTCTGGGCGAAGGCTATGTGGTAGCCTCGGAATCCTGTG
CCCTTACCACTGTGGGGGCCACCTTTGTCCGAGATGTAGAACCCGGCGAAATTATTCGTATTGATCGTGGGGGATTAACC
ACAACCCACGGCCTGACAGCACAATGCCCGGCCCATTGTATTTTTGAATATATTTACTTTGCCCGGCCCGATAGCACAAT
GGATGGCTTTAACGTGAATAAGGTGCGCCGAGAGATGGGGCGTCAATTGGCCCGGGAATACTTGGTGGATGCAGATATTG
TCATACCAGTACCGGATTCAGGTACCGCAGCGGCTAGGGGTTATGCCGAAGAGTCGGGGATACCCTTCGAAGAGGGGCTA
ATGAAAAACCGCTATATTGGCCGGACCTTTATTCAGCCTACCCAAGAAATGCGGGAATTGGGAGTGCGCATGAAGTTAAA
CCCCATTCGTGAGGTGTTGCAGGGCAAACGTGTGGTGATGGTGGATGACTCCATAGTGCGGGGTACCACCAGTCAAAAGC
TTGTAGCCATGCTGCGGGAGGCCGGAGCCAAGGAAGTTCATATGGTCATTAGTTCACCGCCAGTTCTTCGGGCTTGCTAT
TACGGTATTGATACTTCCAACGAACAAGAATTGATTGCAGCCCAAAAGTCTCCAGAGGAAATCTGCCAGAGCATCGAGGC
AGATGGCTTACACTATTTAAGCCTGGAGGGGTTGTTGGATATCTTTGCACCCAAGGAAAAGAACTTCTGTACCGCATGTT
TCAACGGCATTTACCCGGTGGAAGTGCCCAAACAGACGCAGGGCGGTAAGTTCAGTCTGGAATAA

Upstream 100 bases:

>100_bases
TGGTCATCAATGTTGTAAATCCTGGTTGCACCGGTTGCGGCGGCAATTTGATAAATCTATCCGTGTCCAAGCTGGCAGAA
ACCTGGCGGGGGGCGATTGA

Downstream 100 bases:

>100_bases
GGGAGTGTGTCCATGACAGAGCAGAAATCACAACCCCTTACCTATGCTGGGGCGGGGGTCAATATCGAAGCAGGAAACCA
GGCTGTCAGTCTCATGAAGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MFNEIGTILWSDKPVEECGVFGIFAPGLDVARLTYYGLHALQHRGQESAGIAVADGSQIELQKGMGLVPEVFSGHSLDKF
SGFAAIGHVRYSTTGASSPLNAQPLVFRYAKGMLGLAHNGNLTNVADLRAQLASTGSVFQSTTDSEVLVNLIARYNANSV
EEALMKCMIDVKGAYALLIITEDTLYAARDPYGLRPLCLGRLGEGYVVASESCALTTVGATFVRDVEPGEIIRIDRGGLT
TTHGLTAQCPAHCIFEYIYFARPDSTMDGFNVNKVRREMGRQLAREYLVDADIVIPVPDSGTAAARGYAEESGIPFEEGL
MKNRYIGRTFIQPTQEMRELGVRMKLNPIREVLQGKRVVMVDDSIVRGTTSQKLVAMLREAGAKEVHMVISSPPVLRACY
YGIDTSNEQELIAAQKSPEEICQSIEADGLHYLSLEGLLDIFAPKEKNFCTACFNGIYPVEVPKQTQGGKFSLE

Sequences:

>Translated_474_residues
MFNEIGTILWSDKPVEECGVFGIFAPGLDVARLTYYGLHALQHRGQESAGIAVADGSQIELQKGMGLVPEVFSGHSLDKF
SGFAAIGHVRYSTTGASSPLNAQPLVFRYAKGMLGLAHNGNLTNVADLRAQLASTGSVFQSTTDSEVLVNLIARYNANSV
EEALMKCMIDVKGAYALLIITEDTLYAARDPYGLRPLCLGRLGEGYVVASESCALTTVGATFVRDVEPGEIIRIDRGGLT
TTHGLTAQCPAHCIFEYIYFARPDSTMDGFNVNKVRREMGRQLAREYLVDADIVIPVPDSGTAAARGYAEESGIPFEEGL
MKNRYIGRTFIQPTQEMRELGVRMKLNPIREVLQGKRVVMVDDSIVRGTTSQKLVAMLREAGAKEVHMVISSPPVLRACY
YGIDTSNEQELIAAQKSPEEICQSIEADGLHYLSLEGLLDIFAPKEKNFCTACFNGIYPVEVPKQTQGGKFSLE
>Mature_474_residues
MFNEIGTILWSDKPVEECGVFGIFAPGLDVARLTYYGLHALQHRGQESAGIAVADGSQIELQKGMGLVPEVFSGHSLDKF
SGFAAIGHVRYSTTGASSPLNAQPLVFRYAKGMLGLAHNGNLTNVADLRAQLASTGSVFQSTTDSEVLVNLIARYNANSV
EEALMKCMIDVKGAYALLIITEDTLYAARDPYGLRPLCLGRLGEGYVVASESCALTTVGATFVRDVEPGEIIRIDRGGLT
TTHGLTAQCPAHCIFEYIYFARPDSTMDGFNVNKVRREMGRQLAREYLVDADIVIPVPDSGTAAARGYAEESGIPFEEGL
MKNRYIGRTFIQPTQEMRELGVRMKLNPIREVLQGKRVVMVDDSIVRGTTSQKLVAMLREAGAKEVHMVISSPPVLRACY
YGIDTSNEQELIAAQKSPEEICQSIEADGLHYLSLEGLLDIFAPKEKNFCTACFNGIYPVEVPKQTQGGKFSLE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=506, Percent_Identity=41.699604743083, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI4826742, Length=187, Percent_Identity=31.0160427807487, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788651, Length=467, Percent_Identity=41.7558886509636, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI1790167, Length=227, Percent_Identity=33.4801762114537, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17554892, Length=480, Percent_Identity=42.5, Blast_Score=350, Evalue=7e-97,
Organism=Saccharomyces cerevisiae, GI6323958, Length=470, Percent_Identity=37.0212765957447, Blast_Score=265, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24659598, Length=490, Percent_Identity=43.0612244897959, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI28573187, Length=494, Percent_Identity=42.5101214574899, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24659604, Length=465, Percent_Identity=43.2258064516129, Blast_Score=373, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51672; Mature: 51672

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNEIGTILWSDKPVEECGVFGIFAPGLDVARLTYYGLHALQHRGQESAGIAVADGSQIE
CCCCCCEEEECCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCEEE
LQKGMGLVPEVFSGHSLDKFSGFAAIGHVRYSTTGASSPLNAQPLVFRYAKGMLGLAHNG
CCCCCCCCHHHHCCCCCHHHCCEEEEEEEEEECCCCCCCCCCCCHHHHHHCCCEEEECCC
NLTNVADLRAQLASTGSVFQSTTDSEVLVNLIARYNANSVEEALMKCMIDVKGAYALLII
CCCHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEE
TEDTLYAARDPYGLRPLCLGRLGEGYVVASESCALTTVGATFVRDVEPGEIIRIDRGGLT
ECCCEEECCCCCCCCHHHHHCCCCCEEEECCCCEEEEECHHHHCCCCCCCEEEEECCCCC
TTHGLTAQCPAHCIFEYIYFARPDSTMDGFNVNKVRREMGRQLAREYLVDADIVIPVPDS
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCC
GTAAARGYAEESGIPFEEGLMKNRYIGRTFIQPTQEMRELGVRMKLNPIREVLQGKRVVM
CCHHHCCCHHHCCCCHHHHHHHCCCCCHHHCCCHHHHHHCCCCEEEHHHHHHHCCCEEEE
VDDSIVRGTTSQKLVAMLREAGAKEVHMVISSPPVLRACYYGIDTSNEQELIAAQKSPEE
ECCCHHCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHEECCCCCCCHHHHHHCCCHHH
ICQSIEADGLHYLSLEGLLDIFAPKEKNFCTACFNGIYPVEVPKQTQGGKFSLE
HHHHHCCCCEEEEEHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEECC
>Mature Secondary Structure
MFNEIGTILWSDKPVEECGVFGIFAPGLDVARLTYYGLHALQHRGQESAGIAVADGSQIE
CCCCCCEEEECCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCEEE
LQKGMGLVPEVFSGHSLDKFSGFAAIGHVRYSTTGASSPLNAQPLVFRYAKGMLGLAHNG
CCCCCCCCHHHHCCCCCHHHCCEEEEEEEEEECCCCCCCCCCCCHHHHHHCCCEEEECCC
NLTNVADLRAQLASTGSVFQSTTDSEVLVNLIARYNANSVEEALMKCMIDVKGAYALLII
CCCHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEE
TEDTLYAARDPYGLRPLCLGRLGEGYVVASESCALTTVGATFVRDVEPGEIIRIDRGGLT
ECCCEEECCCCCCCCHHHHHCCCCCEEEECCCCEEEEECHHHHCCCCCCCEEEEECCCCC
TTHGLTAQCPAHCIFEYIYFARPDSTMDGFNVNKVRREMGRQLAREYLVDADIVIPVPDS
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCC
GTAAARGYAEESGIPFEEGLMKNRYIGRTFIQPTQEMRELGVRMKLNPIREVLQGKRVVM
CCHHHCCCHHHCCCCHHHHHHHCCCCCHHHCCCHHHHHHCCCCEEEHHHHHHHCCCEEEE
VDDSIVRGTTSQKLVAMLREAGAKEVHMVISSPPVLRACYYGIDTSNEQELIAAQKSPEE
ECCCHHCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHEECCCCCCCHHHHHHCCCHHH
ICQSIEADGLHYLSLEGLLDIFAPKEKNFCTACFNGIYPVEVPKQTQGGKFSLE
HHHHHCCCCEEEEEHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]