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The map label for this gene is ykfC [H]

Identifier: 134300160

GI number: 134300160

Start: 2540178

End: 2540978

Strand: Reverse

Name: ykfC [H]

Synonym: Dred_2321

Alternate gene names: 134300160

Gene position: 2540978-2540178 (Counterclockwise)

Preceding gene: 134300161

Following gene: 134300159

Centisome position: 70.42

GC content: 47.32

Gene sequence:

>801_bases
ATGCCTAACCTGAGAGAGGTAGAGGTGGGTACGACGGTAGTGACAGCGGTGGCTGTGGCAGATATCCTGGAACAACCGGA
GGCAGGAGTTCCACTGGTGACTCAGCAGTTAATGGGGTGGCCTGCCCTGGTAATGGGAGTGGAAGGTGATTGGTTCCATA
TTCAGACCTATGAAGGTTTGCCCGGTTGGGCAAAGATGGATCATTTCTGTGTACCCCAGTGGTCTAAGGAAGGGGCTAAC
GTCCAGGTTATAAGGGCCACTGCCAGGCTAACCAATCATCCCAATAGTCACTATGAGGCTTTATGTACAGTATTTATGGG
AAGCTTGCTCAAGGTTGTGTCCGAAGAGCAAGAATATCTCCAGGTGGCTTTACCCAGGGGTGATTTTGCCTTTTTACCAA
AAATTGTAACCGACAGAATACCTTTAAAGGGCTATGGACTTAGGAAAGGCATACTGACTGCTGCAGGTTTATTATCCGGA
GTACCATACCTTTGGGGTGGCATGACTGTCCGAGGGGTGGATTGTTCAGGTCTAACCTACATGGCTTATTTGGTTAATGG
TTATCAGCTTCCAAGGAATGCCCAGGATCAGTTTAGGATTGGTAAGCCTGTAGAAATAGAAGAACTGGAAATGGGCGACC
TGGTATTTTTTTCCACAGTCGATCCAGGACCCTCTCACGTGGGAATATACCAGGGGGATGGCCTGTTTATTAATGCCCGT
TCAAAGGAAGGGGTAACAATTACATCCTTTGATGATTCTTTTTTTGTTAACCGTTATCTGGGAGGTAGGAGGTATCTTTA
G

Upstream 100 bases:

>100_bases
AAATGTTGAATGGTCAACCGGAGTTTGGTAATTATACCGTAGAGAGCATAACAAAGTAAGCAATCGAATATGGTTTAGGT
AAAAGGCGAGGGGTGATTCT

Downstream 100 bases:

>100_bases
GTAATTGTTGAAAAAATATCCTAACCAGTTAATTATTTGGTATAATAATGGTAGAACTTCATGTGCGGGAGCGTAAGAAT
ATGCAAGCAACTATGGAAAT

Product: NLP/P60 protein

Products: NA

Alternate protein names: Cell wall endopeptidase ykfC [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MPNLREVEVGTTVVTAVAVADILEQPEAGVPLVTQQLMGWPALVMGVEGDWFHIQTYEGLPGWAKMDHFCVPQWSKEGAN
VQVIRATARLTNHPNSHYEALCTVFMGSLLKVVSEEQEYLQVALPRGDFAFLPKIVTDRIPLKGYGLRKGILTAAGLLSG
VPYLWGGMTVRGVDCSGLTYMAYLVNGYQLPRNAQDQFRIGKPVEIEELEMGDLVFFSTVDPGPSHVGIYQGDGLFINAR
SKEGVTITSFDDSFFVNRYLGGRRYL

Sequences:

>Translated_266_residues
MPNLREVEVGTTVVTAVAVADILEQPEAGVPLVTQQLMGWPALVMGVEGDWFHIQTYEGLPGWAKMDHFCVPQWSKEGAN
VQVIRATARLTNHPNSHYEALCTVFMGSLLKVVSEEQEYLQVALPRGDFAFLPKIVTDRIPLKGYGLRKGILTAAGLLSG
VPYLWGGMTVRGVDCSGLTYMAYLVNGYQLPRNAQDQFRIGKPVEIEELEMGDLVFFSTVDPGPSHVGIYQGDGLFINAR
SKEGVTITSFDDSFFVNRYLGGRRYL
>Mature_265_residues
PNLREVEVGTTVVTAVAVADILEQPEAGVPLVTQQLMGWPALVMGVEGDWFHIQTYEGLPGWAKMDHFCVPQWSKEGANV
QVIRATARLTNHPNSHYEALCTVFMGSLLKVVSEEQEYLQVALPRGDFAFLPKIVTDRIPLKGYGLRKGILTAAGLLSGV
PYLWGGMTVRGVDCSGLTYMAYLVNGYQLPRNAQDQFRIGKPVEIEELEMGDLVFFSTVDPGPSHVGIYQGDGLFINARS
KEGVTITSFDDSFFVNRYLGGRRYL

Specific function: Specifically hydrolyzes gamma-D-glutamyl-L-lysine bonds in murein peptides, releasing L-Ala-D-Glu [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=114, Percent_Identity=43.859649122807, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1788501, Length=108, Percent_Identity=39.8148148148148, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788001, Length=107, Percent_Identity=41.1214953271028, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1786421, Length=113, Percent_Identity=38.0530973451327, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 29378; Mature: 29247

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNLREVEVGTTVVTAVAVADILEQPEAGVPLVTQQLMGWPALVMGVEGDWFHIQTYEGL
CCCCEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEECCCCEEEEEEECCC
PGWAKMDHFCVPQWSKEGANVQVIRATARLTNHPNSHYEALCTVFMGSLLKVVSEEQEYL
CCHHCCCCEECCCCCCCCCCEEEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHEE
QVALPRGDFAFLPKIVTDRIPLKGYGLRKGILTAAGLLSGVPYLWGGMTVRGVDCSGLTY
EEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCHHH
MAYLVNGYQLPRNAQDQFRIGKPVEIEELEMGDLVFFSTVDPGPSHVGIYQGDGLFINAR
EEHHHCCCCCCCCCHHHHCCCCCCCHHEECCCCEEEEEECCCCCCEEEEEECCCEEEECC
SKEGVTITSFDDSFFVNRYLGGRRYL
CCCCCEEEECCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
PNLREVEVGTTVVTAVAVADILEQPEAGVPLVTQQLMGWPALVMGVEGDWFHIQTYEGL
CCCEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEECCCCEEEEEEECCC
PGWAKMDHFCVPQWSKEGANVQVIRATARLTNHPNSHYEALCTVFMGSLLKVVSEEQEYL
CCHHCCCCEECCCCCCCCCCEEEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHEE
QVALPRGDFAFLPKIVTDRIPLKGYGLRKGILTAAGLLSGVPYLWGGMTVRGVDCSGLTY
EEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCHHH
MAYLVNGYQLPRNAQDQFRIGKPVEIEELEMGDLVFFSTVDPGPSHVGIYQGDGLFINAR
EEHHHCCCCCCCCCHHHHCCCCCCCHHEECCCCEEEEEECCCCCCEEEEEECCCEEEECC
SKEGVTITSFDDSFFVNRYLGGRRYL
CCCCCEEEECCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]