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The map label for this gene is moaA

Identifier: 134300107

GI number: 134300107

Start: 2479967

End: 2480944

Strand: Reverse

Name: moaA

Synonym: Dred_2268

Alternate gene names: 134300107

Gene position: 2480944-2479967 (Counterclockwise)

Preceding gene: 134300108

Following gene: 134300106

Centisome position: 68.76

GC content: 45.09

Gene sequence:

>978_bases
ATGATTGATAATTATAATCGAAACATCAACTATCTGCGCATATCAGTGACAGATCGGTGCAACCTGCGCTGTGTTTACTG
TATGCCCCCGGAAGGTGTTAAACAAACCCCCCACTCTGAAATACTAAGTCTGGAGGAATTTGCCCGGGTGGTGGATGCCG
CGTCTGATATTGGTATTCGCAAAATCCGTATCACCGGTGGGGAGCCACTGGTGAGAAAAAATATTGTCAACTTATTCGAA
AAGATTTCCACTAACTCAGCCATTGATGATATTTCTTTGACTACCAATGGCGTACTCTTTGCAGAAATGGCGTCAGATTT
AAAAAAAGCCGGGCTGAACCGGGTAAATTTTAGTTTAGATAGCCTGAACCCGGATACCTTTCGGGATATAACCCGGATGG
GTAAGTTTAATGATGTTTGGCGCAGTATTCAGAAGGCCCTGGAACTGGAACTCCACCCGGTCAAATTAAACGTGGTGGCG
GTACGGGGAATTAATGACCATGAATTTGCGGACTTTGCCCGGTTAACTAAAGAAATACCTATCCACGTAAGGTTTATTGA
ATTAATGCCTATTGGGGAATGCAATCCTTGGGCAGTGGGGAATTTTATAGCTGCGGAGGAAATCTTGCATGGCTTGCAAC
AGAAATTTGGCCTATTGGATACACAGGTGAAGGTGACTGGCAGTGGTCCTGCCAAATACTACTGCTTGCCGAATTCCAAA
GGGACCATTGGGTTTATTACTGCCATCAGCGAACACTTCTGTGCCGGTTGTAACCGACTGCGTCTCACTGCCAATGGTCA
GTTACGTCCTTGCCTTTATGGCAAGCAGGAGTTTGACTTAAAAACTCCTTTAAGAGAGGGTGCTAGTAGGCAAGAATTAG
CCAAAATCATTACCAAAGCAATTCGACATAAACCCAGTCAACACCATATGGAAGATGGCTGGAGGGATCGTCGTGTTATG
AGTCAGATAGGGGGATAA

Upstream 100 bases:

>100_bases
TCTGCTTAAAAAAACTATAAAAAACAGTTGTTTACCCCAAGGAGAAATTAATAATTTAACAGATATAACTTCTATCTTAA
CCTTATAAGGGGGCTGGACA

Downstream 100 bases:

>100_bases
ATATTGTCCGAGTTAACACATTTCGATGCCAAGGGTAATGCCTGGATGGTGGATATAACAGAAAAAGAAGAAACCCATCG
TGTGGCCGAGGTCAGGGGAG

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MIDNYNRNINYLRISVTDRCNLRCVYCMPPEGVKQTPHSEILSLEEFARVVDAASDIGIRKIRITGGEPLVRKNIVNLFE
KISTNSAIDDISLTTNGVLFAEMASDLKKAGLNRVNFSLDSLNPDTFRDITRMGKFNDVWRSIQKALELELHPVKLNVVA
VRGINDHEFADFARLTKEIPIHVRFIELMPIGECNPWAVGNFIAAEEILHGLQQKFGLLDTQVKVTGSGPAKYYCLPNSK
GTIGFITAISEHFCAGCNRLRLTANGQLRPCLYGKQEFDLKTPLREGASRQELAKIITKAIRHKPSQHHMEDGWRDRRVM
SQIGG

Sequences:

>Translated_325_residues
MIDNYNRNINYLRISVTDRCNLRCVYCMPPEGVKQTPHSEILSLEEFARVVDAASDIGIRKIRITGGEPLVRKNIVNLFE
KISTNSAIDDISLTTNGVLFAEMASDLKKAGLNRVNFSLDSLNPDTFRDITRMGKFNDVWRSIQKALELELHPVKLNVVA
VRGINDHEFADFARLTKEIPIHVRFIELMPIGECNPWAVGNFIAAEEILHGLQQKFGLLDTQVKVTGSGPAKYYCLPNSK
GTIGFITAISEHFCAGCNRLRLTANGQLRPCLYGKQEFDLKTPLREGASRQELAKIITKAIRHKPSQHHMEDGWRDRRVM
SQIGG
>Mature_325_residues
MIDNYNRNINYLRISVTDRCNLRCVYCMPPEGVKQTPHSEILSLEEFARVVDAASDIGIRKIRITGGEPLVRKNIVNLFE
KISTNSAIDDISLTTNGVLFAEMASDLKKAGLNRVNFSLDSLNPDTFRDITRMGKFNDVWRSIQKALELELHPVKLNVVA
VRGINDHEFADFARLTKEIPIHVRFIELMPIGECNPWAVGNFIAAEEILHGLQQKFGLLDTQVKVTGSGPAKYYCLPNSK
GTIGFITAISEHFCAGCNRLRLTANGQLRPCLYGKQEFDLKTPLREGASRQELAKIITKAIRHKPSQHHMEDGWRDRRVM
SQIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=308, Percent_Identity=44.1558441558442, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI22027536, Length=308, Percent_Identity=44.1558441558442, Blast_Score=268, Evalue=5e-72,
Organism=Escherichia coli, GI1786999, Length=314, Percent_Identity=35.031847133758, Blast_Score=205, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI32566329, Length=328, Percent_Identity=40.2439024390244, Blast_Score=249, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI32566331, Length=311, Percent_Identity=41.1575562700965, Blast_Score=247, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI17561210, Length=327, Percent_Identity=38.2262996941896, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI28574490, Length=312, Percent_Identity=40.3846153846154, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI28574488, Length=331, Percent_Identity=39.5770392749245, Blast_Score=242, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_DESRM (A4J6S5)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113603.1
- ProteinModelPortal:   A4J6S5
- SMR:   A4J6S5
- STRING:   A4J6S5
- GeneID:   4955732
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_2268
- eggNOG:   COG2896
- HOGENOM:   HBG529885
- OMA:   PMARHFR
- PhylomeDB:   A4J6S5
- BioCyc:   DRED349161:DRED_2268-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 36590; Mature: 36590

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 13-13 BINDING 26-26 BINDING 63-63 BINDING 67-67 BINDING 94-94 BINDING 118-118 BINDING 155-155 BINDING 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDNYNRNINYLRISVTDRCNLRCVYCMPPEGVKQTPHSEILSLEEFARVVDAASDIGIR
CCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
KIRITGGEPLVRKNIVNLFEKISTNSAIDDISLTTNGVLFAEMASDLKKAGLNRVNFSLD
EEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCEEHHHHHHHHHHCCCCEEEEEEC
SLNPDTFRDITRMGKFNDVWRSIQKALELELHPVKLNVVAVRGINDHEFADFARLTKEIP
CCCCHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEEEEEEEEECCCCHHHHHHHHHHHCCC
IHVRFIELMPIGECNPWAVGNFIAAEEILHGLQQKFGLLDTQVKVTGSGPAKYYCLPNSK
EEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCC
GTIGFITAISEHFCAGCNRLRLTANGQLRPCLYGKQEFDLKTPLREGASRQELAKIITKA
CCEEHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCCCHHHCCCCHHHHHHHHHHH
IRHKPSQHHMEDGWRDRRVMSQIGG
HHCCCCHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MIDNYNRNINYLRISVTDRCNLRCVYCMPPEGVKQTPHSEILSLEEFARVVDAASDIGIR
CCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
KIRITGGEPLVRKNIVNLFEKISTNSAIDDISLTTNGVLFAEMASDLKKAGLNRVNFSLD
EEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCEEHHHHHHHHHHCCCCEEEEEEC
SLNPDTFRDITRMGKFNDVWRSIQKALELELHPVKLNVVAVRGINDHEFADFARLTKEIP
CCCCHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEEEEEEEEECCCCHHHHHHHHHHHCCC
IHVRFIELMPIGECNPWAVGNFIAAEEILHGLQQKFGLLDTQVKVTGSGPAKYYCLPNSK
EEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCC
GTIGFITAISEHFCAGCNRLRLTANGQLRPCLYGKQEFDLKTPLREGASRQELAKIITKA
CCEEHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCCCHHHCCCCHHHHHHHHHHH
IRHKPSQHHMEDGWRDRRVMSQIGG
HHCCCCHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA