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The map label for this gene is braE [H]

Identifier: 134300080

GI number: 134300080

Start: 2446871

End: 2447851

Strand: Reverse

Name: braE [H]

Synonym: Dred_2238

Alternate gene names: 134300080

Gene position: 2447851-2446871 (Counterclockwise)

Preceding gene: 134300081

Following gene: 134300079

Centisome position: 67.84

GC content: 41.18

Gene sequence:

>981_bases
ATGTTGATGTTTAAGCGAAATCTAGGCAAACAAATTGTTATTTTTATGGCCTTGCTGGCTATTTGGGGTAGCGTTTATTT
ATTAATCCAAACCGGCATTATCAATCCCTTTTATGAACTTAACCTAATATTAATCGGTATCAATATTATTCTAGCGGTGA
GCCTAAACCTAATTAATGGTTTTAACGGACAGTTTTCCATCGGTCACGCTGGTTTTATGGCTATCGGAGCCTATGGGGCA
GCTATTATTACTATCAAAGCAGGACTACCCTTCCCTGTGGCTCTTTTATTTGGGGCAGTTGTTGCAGGAATTTTCGGTAT
TCTGGTTGGTCTGCCTACCTTGCGTCTACGGGGTGACTACTTAGCAATTGCTACCCTTGGTTTTGGAGAAATTATTCGCG
GTGTCATTGTTAATATTGACTACATCGGCGGTGCCTATGGCCTTTCCGGCATCCCTAAAATGGCTAATTGGACTTGGGTG
TTCTGGTTAACTGTATTAACGGTAGTAATTATTACCAACTTTATAAATTCCACCCACGGCAGGGCAACTGTATCCATTAG
GGAGAATGAGATTGCCGCCGAGGCCATGGGCATAAATACCACTAAATACAAGGTTATGGCTTTTACCATTGGTTCATTTT
TTGCTGGTCTTGCTGGAGGATTACATGCCCATTATTTCTATACCATTCAGCCGGGTACATTTAACTTCCTTAAATCCTTT
GATATTTTGGTAATGGTTGTTTTGGGTGGGCAAGGTAGTATTACTGGTTCGATTATCGCTGCCATTGGCCTTACTATTTT
ATCGGCCTCTCTACAAAGTCTGGTTTCTCTACGTTTGGTTATTTACGCCTTGTTATTGATTATCATTATGATATTTAGAC
CCCAAGGTCTCATGGGCACCAAGGAATTTAGTTTGAGTGTGTTTGGCGAAAAATCGAAGGATAAGGGGGAGAAACAGCAT
GGCACTTCTATCGGTCAGTAA

Upstream 100 bases:

>100_bases
CTTGTACCGTGATCCTGCTGCCTTCATCATTCTAATTATTATCCTCATAGTCTTGCCCGCCGGCTTGTTTGGCAAGAATG
TCCGGGAGAAAGTGTAGGTG

Downstream 100 bases:

>100_bases
CCTGTCCATATCTTTCGGTGGCCTAAAGGCTGTATCAAATGTAAATTTTGAACTGAATAAAAATGATTTAGCAGGTCTCA
TTGGGCCCAACGGGGCAGGT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MLMFKRNLGKQIVIFMALLAIWGSVYLLIQTGIINPFYELNLILIGINIILAVSLNLINGFNGQFSIGHAGFMAIGAYGA
AIITIKAGLPFPVALLFGAVVAGIFGILVGLPTLRLRGDYLAIATLGFGEIIRGVIVNIDYIGGAYGLSGIPKMANWTWV
FWLTVLTVVIITNFINSTHGRATVSIRENEIAAEAMGINTTKYKVMAFTIGSFFAGLAGGLHAHYFYTIQPGTFNFLKSF
DILVMVVLGGQGSITGSIIAAIGLTILSASLQSLVSLRLVIYALLLIIIMIFRPQGLMGTKEFSLSVFGEKSKDKGEKQH
GTSIGQ

Sequences:

>Translated_326_residues
MLMFKRNLGKQIVIFMALLAIWGSVYLLIQTGIINPFYELNLILIGINIILAVSLNLINGFNGQFSIGHAGFMAIGAYGA
AIITIKAGLPFPVALLFGAVVAGIFGILVGLPTLRLRGDYLAIATLGFGEIIRGVIVNIDYIGGAYGLSGIPKMANWTWV
FWLTVLTVVIITNFINSTHGRATVSIRENEIAAEAMGINTTKYKVMAFTIGSFFAGLAGGLHAHYFYTIQPGTFNFLKSF
DILVMVVLGGQGSITGSIIAAIGLTILSASLQSLVSLRLVIYALLLIIIMIFRPQGLMGTKEFSLSVFGEKSKDKGEKQH
GTSIGQ
>Mature_326_residues
MLMFKRNLGKQIVIFMALLAIWGSVYLLIQTGIINPFYELNLILIGINIILAVSLNLINGFNGQFSIGHAGFMAIGAYGA
AIITIKAGLPFPVALLFGAVVAGIFGILVGLPTLRLRGDYLAIATLGFGEIIRGVIVNIDYIGGAYGLSGIPKMANWTWV
FWLTVLTVVIITNFINSTHGRATVSIRENEIAAEAMGINTTKYKVMAFTIGSFFAGLAGGLHAHYFYTIQPGTFNFLKSF
DILVMVVLGGQGSITGSIIAAIGLTILSASLQSLVSLRLVIYALLLIIIMIFRPQGLMGTKEFSLSVFGEKSKDKGEKQH
GTSIGQ

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=307, Percent_Identity=35.1791530944625, Blast_Score=147, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 34973; Mature: 34973

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS00046 HISTONE_H2A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMFKRNLGKQIVIFMALLAIWGSVYLLIQTGIINPFYELNLILIGINIILAVSLNLING
CEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEHHHHHHHHHHHHHCC
FNGQFSIGHAGFMAIGAYGAAIITIKAGLPFPVALLFGAVVAGIFGILVGLPTLRLRGDY
CCCEEEECCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCE
LAIATLGFGEIIRGVIVNIDYIGGAYGLSGIPKMANWTWVFWLTVLTVVIITNFINSTHG
EEEEECCHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
RATVSIRENEIAAEAMGINTTKYKVMAFTIGSFFAGLAGGLHAHYFYTIQPGTFNFLKSF
EEEEEEECCHHHHHHHCCCCCCEEEEEEEHHHHHHHHHCCCEEEEEEEECCCCHHHHHHH
DILVMVVLGGQGSITGSIIAAIGLTILSASLQSLVSLRLVIYALLLIIIMIFRPQGLMGT
HHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KEFSLSVFGEKSKDKGEKQHGTSIGQ
CCEEEEEECCCCCCCCCHHCCCCCCC
>Mature Secondary Structure
MLMFKRNLGKQIVIFMALLAIWGSVYLLIQTGIINPFYELNLILIGINIILAVSLNLING
CEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEHHHHHHHHHHHHHCC
FNGQFSIGHAGFMAIGAYGAAIITIKAGLPFPVALLFGAVVAGIFGILVGLPTLRLRGDY
CCCEEEECCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCE
LAIATLGFGEIIRGVIVNIDYIGGAYGLSGIPKMANWTWVFWLTVLTVVIITNFINSTHG
EEEEECCHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
RATVSIRENEIAAEAMGINTTKYKVMAFTIGSFFAGLAGGLHAHYFYTIQPGTFNFLKSF
EEEEEEECCHHHHHHHCCCCCCEEEEEEEHHHHHHHHHCCCEEEEEEEECCCCHHHHHHH
DILVMVVLGGQGSITGSIIAAIGLTILSASLQSLVSLRLVIYALLLIIIMIFRPQGLMGT
HHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KEFSLSVFGEKSKDKGEKQHGTSIGQ
CCEEEEEECCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]