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The map label for this gene is metH [H]

Identifier: 134300051

GI number: 134300051

Start: 2414799

End: 2417201

Strand: Reverse

Name: metH [H]

Synonym: Dred_2207

Alternate gene names: 134300051

Gene position: 2417201-2414799 (Counterclockwise)

Preceding gene: 134300052

Following gene: 134300050

Centisome position: 66.99

GC content: 48.31

Gene sequence:

>2403_bases
TTGTCTTTTAGGAAAAGAGTAAAAAAAGAAGTTCTCATCATTGATGGCGCAATGGGTACACTGTTGCAGCAGAGGGGTCT
GCCAGGGGGTTGGTGCCCTGAGGAATGGAACCTTAGTCACCCGGAAGCCGTTAAAGAGATACATAAACTTTATTTAGAAG
CAGGGGCTGACATTATCACCACCAACACCTTTGGGGCCATACAATTAAAACTGGCAGATTATCATTTGGGTGATCAGGTT
AAGGAGATTAATCAGGCAGCAGTTAAACTGGCAAAGGAGGTGGCCCAGCCCTACGGGGCTATGGTAGCCGGTTCCGTGGG
ACCCTTAGGTAAGTTTTTACAACCACTGGGCACCATGACCTTTGAAGAAGCTTATCAGCAGTTTTATGAGCAATGTGCGG
CGATGGTGGAAGCCGGTGTGGATTTAATTCTCTTTGAAACCTTTGGAGATATTGGAGAAATGCGTGCAGCCCTTATTGCT
GCGGCCGACGCCGGAGATGTACCGGTGGTGGCCAGTTTCACCTTTGATGAAACTGGCAGGACCTTTACAGGAACAGACCC
GGAGACTGCTGCGGTGGTGGCAGAAAGGCTTGGTATTGCAGCCATTGGGGTCAATTGCTCGGTGGGGCCCCGTCAATTGG
AGGGTGTGGTCAGGAAGCTTACAGAAAGTACTAATTTACCGGTGTTGGTTTCACCCAATGCGGGTATGCCAGAAATTATT
GGAGGAAAAACAGTTTTTAGGGAAACACCGGAGATTATGGCGGAGTATGCACAACGTTTCGTGGATTATGGGGCATCCAT
TTTAGGAGGCTGCTGTGGAACCACGCCAAAACATATCCAGGCCATTTACCAACAGGTGAAAAAACTAAAACCAAAGTGTC
GCAATAAAAGGTTTGGTCTGAGGGTGGCCAGTCGAGTAAAGACCGTAACCATCCAGCCCAGCGGAGTTCCTGTGGCCATT
GGGGAGCGCCTTAATCCTACAGGTAAAAAGGCCCTACAGGCAGAAATTCGGGAGGGAAAAACAGAGATTACCCGCCGGGA
TGCCTTGACTCAGGTACAGGCAGGGGCAGAGATTTTAGATGTTAATGTCGGTGTGGGGGGAGTCGATCAGGTGGCTGCCA
TGGAACGGGCTGTCCAAGCGGTACAGGTTTTGGTAGAGGCACCCCTTTGTATTGACAGCACCGACCCCACAGTCATTGAA
CATGCCTTAAAACTTTATCATGGTAAAGCCCTTATTAATTCTGTGAACGGTGAGGAGAACAGCATTGAAACCATTCTTCC
TTTGGCCAAGCGATATGGAGCTGCCCTCATTGGACTTACTCTGGATGATGAAGGAATCCCACCCACAGCTGCAGGCAGAT
TGGCAGTGGCCCGCAGACTGGTAGAAAGAACCGAGGCAATGGGTATCCCAAGAGATGATTTGATGATGGATTGCCTGGTG
CTAACTGTCAGTGCCCAACCGGAAGGAGTGCCGGAGACGCTGGAAGCCATCCGGCTGGTTAAGCAGAAACTGCAAATCAC
CACTAGTTTGGGAGTTAGTAATGTGTCCTTTGGGTTACCTAACCGTGTATTAATAAATGCGGCCTTCTTTGCCATGGCCT
TAAACGCAGGTTTAGATGCAGCCATCCTAAATCCTCAGGATCAGCGTATGATGGAGACCCTTAGGGCTTCCGCAGTTCTT
ACCGGTAGAGACGTACGGGCAGAAAAATACATTGCCATAGAACAACAGACCCACAATGGTAGCAAAATGGAAACAACGGG
TGAACAGATGAAGGCTTCCACTCCCCTGGATATTTTGTATCAAGCAGTGTTGTCCGGAGATAAAGATAATATTGTTTCCT
ATATTAATGAAGCTCTGAAAACAGGTTTGTCGCCCATGGAACTGCTGGACAAGGCTTTGATACCAGGAATCGAAGAAGTG
GGACGGAGGTATGGAGCAGGTATTTACTTTTTACCTCAACTGATGATGGCTGGAGAAACCATGACCAAAAGCTTTGAACG
ACTTAGACCGGAATTAGCCAAAGGACCTGCCAAGAAGGCGGGTACCGTTGTACTGGCCACTGTTAAGGGAGATATTCACG
ACATTGGCAAAAACATTGTTTCGGTCATGCTGGCCAACCACGGCTTCGAGGTCATTGATCTGGGAAAAAATGTAGCCAAT
GAGGAGATCCTCCGGGTGGCTGCAGAACAAAAGGCAAATATTATAGGGCTGAGTGCCCTTATGACCACTACAATGGTGCA
TATGCAGGAAATAATCAAGTTGGTCAAAGAACAAGGTCTAAACTGCAAAGTAATGGTAGGGGGCGCCGCAGTTACAGAAC
ATTATGCAAGGGAGATTGGTGCAGACGGTTATGCCATTGATGCGGTAGAAGCTGTGAATGTTGCCAAAAAATTAGCCAGT
TAG

Upstream 100 bases:

>100_bases
TAGGTTGGAGTTCCCAACCTAGTAAATACCATAAAAAATGTGATTTTTGCCATAAAACAGACTGCCAATATCGTAGTTAG
GATGGAAGGGGAGAAAGAAA

Downstream 100 bases:

>100_bases
AGGTAAGCTACTTTGGATAAAATAAAGGTTATCTTTCAACCAGTGGGAGTTACTGTGGAAGTGCCGGTTGGTATCACAAT
TTTGGAAGCTGCCAGGTTAG

Product: homocysteine S-methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 800; Mature: 799

Protein sequence:

>800_residues
MSFRKRVKKEVLIIDGAMGTLLQQRGLPGGWCPEEWNLSHPEAVKEIHKLYLEAGADIITTNTFGAIQLKLADYHLGDQV
KEINQAAVKLAKEVAQPYGAMVAGSVGPLGKFLQPLGTMTFEEAYQQFYEQCAAMVEAGVDLILFETFGDIGEMRAALIA
AADAGDVPVVASFTFDETGRTFTGTDPETAAVVAERLGIAAIGVNCSVGPRQLEGVVRKLTESTNLPVLVSPNAGMPEII
GGKTVFRETPEIMAEYAQRFVDYGASILGGCCGTTPKHIQAIYQQVKKLKPKCRNKRFGLRVASRVKTVTIQPSGVPVAI
GERLNPTGKKALQAEIREGKTEITRRDALTQVQAGAEILDVNVGVGGVDQVAAMERAVQAVQVLVEAPLCIDSTDPTVIE
HALKLYHGKALINSVNGEENSIETILPLAKRYGAALIGLTLDDEGIPPTAAGRLAVARRLVERTEAMGIPRDDLMMDCLV
LTVSAQPEGVPETLEAIRLVKQKLQITTSLGVSNVSFGLPNRVLINAAFFAMALNAGLDAAILNPQDQRMMETLRASAVL
TGRDVRAEKYIAIEQQTHNGSKMETTGEQMKASTPLDILYQAVLSGDKDNIVSYINEALKTGLSPMELLDKALIPGIEEV
GRRYGAGIYFLPQLMMAGETMTKSFERLRPELAKGPAKKAGTVVLATVKGDIHDIGKNIVSVMLANHGFEVIDLGKNVAN
EEILRVAAEQKANIIGLSALMTTTMVHMQEIIKLVKEQGLNCKVMVGGAAVTEHYAREIGADGYAIDAVEAVNVAKKLAS

Sequences:

>Translated_800_residues
MSFRKRVKKEVLIIDGAMGTLLQQRGLPGGWCPEEWNLSHPEAVKEIHKLYLEAGADIITTNTFGAIQLKLADYHLGDQV
KEINQAAVKLAKEVAQPYGAMVAGSVGPLGKFLQPLGTMTFEEAYQQFYEQCAAMVEAGVDLILFETFGDIGEMRAALIA
AADAGDVPVVASFTFDETGRTFTGTDPETAAVVAERLGIAAIGVNCSVGPRQLEGVVRKLTESTNLPVLVSPNAGMPEII
GGKTVFRETPEIMAEYAQRFVDYGASILGGCCGTTPKHIQAIYQQVKKLKPKCRNKRFGLRVASRVKTVTIQPSGVPVAI
GERLNPTGKKALQAEIREGKTEITRRDALTQVQAGAEILDVNVGVGGVDQVAAMERAVQAVQVLVEAPLCIDSTDPTVIE
HALKLYHGKALINSVNGEENSIETILPLAKRYGAALIGLTLDDEGIPPTAAGRLAVARRLVERTEAMGIPRDDLMMDCLV
LTVSAQPEGVPETLEAIRLVKQKLQITTSLGVSNVSFGLPNRVLINAAFFAMALNAGLDAAILNPQDQRMMETLRASAVL
TGRDVRAEKYIAIEQQTHNGSKMETTGEQMKASTPLDILYQAVLSGDKDNIVSYINEALKTGLSPMELLDKALIPGIEEV
GRRYGAGIYFLPQLMMAGETMTKSFERLRPELAKGPAKKAGTVVLATVKGDIHDIGKNIVSVMLANHGFEVIDLGKNVAN
EEILRVAAEQKANIIGLSALMTTTMVHMQEIIKLVKEQGLNCKVMVGGAAVTEHYAREIGADGYAIDAVEAVNVAKKLAS
>Mature_799_residues
SFRKRVKKEVLIIDGAMGTLLQQRGLPGGWCPEEWNLSHPEAVKEIHKLYLEAGADIITTNTFGAIQLKLADYHLGDQVK
EINQAAVKLAKEVAQPYGAMVAGSVGPLGKFLQPLGTMTFEEAYQQFYEQCAAMVEAGVDLILFETFGDIGEMRAALIAA
ADAGDVPVVASFTFDETGRTFTGTDPETAAVVAERLGIAAIGVNCSVGPRQLEGVVRKLTESTNLPVLVSPNAGMPEIIG
GKTVFRETPEIMAEYAQRFVDYGASILGGCCGTTPKHIQAIYQQVKKLKPKCRNKRFGLRVASRVKTVTIQPSGVPVAIG
ERLNPTGKKALQAEIREGKTEITRRDALTQVQAGAEILDVNVGVGGVDQVAAMERAVQAVQVLVEAPLCIDSTDPTVIEH
ALKLYHGKALINSVNGEENSIETILPLAKRYGAALIGLTLDDEGIPPTAAGRLAVARRLVERTEAMGIPRDDLMMDCLVL
TVSAQPEGVPETLEAIRLVKQKLQITTSLGVSNVSFGLPNRVLINAAFFAMALNAGLDAAILNPQDQRMMETLRASAVLT
GRDVRAEKYIAIEQQTHNGSKMETTGEQMKASTPLDILYQAVLSGDKDNIVSYINEALKTGLSPMELLDKALIPGIEEVG
RRYGAGIYFLPQLMMAGETMTKSFERLRPELAKGPAKKAGTVVLATVKGDIHDIGKNIVSVMLANHGFEVIDLGKNVANE
EILRVAAEQKANIIGLSALMTTTMVHMQEIIKLVKEQGLNCKVMVGGAAVTEHYAREIGADGYAIDAVEAVNVAKKLAS

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=872, Percent_Identity=30.1605504587156, Blast_Score=338, Evalue=2e-92,
Organism=Homo sapiens, GI157266337, Length=302, Percent_Identity=28.8079470198675, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI13162290, Length=297, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI1790450, Length=873, Percent_Identity=32.3024054982818, Blast_Score=348, Evalue=5e-97,
Organism=Escherichia coli, GI1786456, Length=297, Percent_Identity=29.2929292929293, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17535405, Length=878, Percent_Identity=31.4350797266515, Blast_Score=368, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324982, Length=314, Percent_Identity=26.4331210191083, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322966, Length=316, Percent_Identity=27.8481012658228, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20129603, Length=332, Percent_Identity=27.710843373494, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24585077, Length=289, Percent_Identity=26.643598615917, Blast_Score=69, Evalue=2e-11,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 85900; Mature: 85768

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFRKRVKKEVLIIDGAMGTLLQQRGLPGGWCPEEWNLSHPEAVKEIHKLYLEAGADIIT
CCHHHHHHHHEEEEECHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
TNTFGAIQLKLADYHLGDQVKEINQAAVKLAKEVAQPYGAMVAGSVGPLGKFLQPLGTMT
ECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHH
FEEAYQQFYEQCAAMVEAGVDLILFETFGDIGEMRAALIAAADAGDVPVVASFTFDETGR
HHHHHHHHHHHHHHHHHCCCCEEEEHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
TFTGTDPETAAVVAERLGIAAIGVNCSVGPRQLEGVVRKLTESTNLPVLVSPNAGMPEII
EECCCCCHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
GGKTVFRETPEIMAEYAQRFVDYGASILGGCCGTTPKHIQAIYQQVKKLKPKCRNKRFGL
CCCHHHHCCHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHCHHHCCCCCCH
RVASRVKTVTIQPSGVPVAIGERLNPTGKKALQAEIREGKTEITRRDALTQVQAGAEILD
HHHCCEEEEEECCCCCCEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEE
VNVGVGGVDQVAAMERAVQAVQVLVEAPLCIDSTDPTVIEHALKLYHGKALINSVNGEEN
EECCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
SIETILPLAKRYGAALIGLTLDDEGIPPTAAGRLAVARRLVERTEAMGIPRDDLMMDCLV
HHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LTVSAQPEGVPETLEAIRLVKQKLQITTSLGVSNVSFGLPNRVLINAAFFAMALNAGLDA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCE
AILNPQDQRMMETLRASAVLTGRDVRAEKYIAIEQQTHNGSKMETTGEQMKASTPLDILY
EEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCCCCHHHHH
QAVLSGDKDNIVSYINEALKTGLSPMELLDKALIPGIEEVGRRYGAGIYFLPQLMMAGET
HHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCHH
MTKSFERLRPELAKGPAKKAGTVVLATVKGDIHDIGKNIVSVMLANHGFEVIDLGKNVAN
HHHHHHHHCHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCCHHH
EEILRVAAEQKANIIGLSALMTTTMVHMQEIIKLVKEQGLNCKVMVGGAAVTEHYAREIG
HHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHC
ADGYAIDAVEAVNVAKKLAS
CCCCEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
SFRKRVKKEVLIIDGAMGTLLQQRGLPGGWCPEEWNLSHPEAVKEIHKLYLEAGADIIT
CHHHHHHHHEEEEECHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
TNTFGAIQLKLADYHLGDQVKEINQAAVKLAKEVAQPYGAMVAGSVGPLGKFLQPLGTMT
ECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHH
FEEAYQQFYEQCAAMVEAGVDLILFETFGDIGEMRAALIAAADAGDVPVVASFTFDETGR
HHHHHHHHHHHHHHHHHCCCCEEEEHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
TFTGTDPETAAVVAERLGIAAIGVNCSVGPRQLEGVVRKLTESTNLPVLVSPNAGMPEII
EECCCCCHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
GGKTVFRETPEIMAEYAQRFVDYGASILGGCCGTTPKHIQAIYQQVKKLKPKCRNKRFGL
CCCHHHHCCHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHCHHHCCCCCCH
RVASRVKTVTIQPSGVPVAIGERLNPTGKKALQAEIREGKTEITRRDALTQVQAGAEILD
HHHCCEEEEEECCCCCCEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEE
VNVGVGGVDQVAAMERAVQAVQVLVEAPLCIDSTDPTVIEHALKLYHGKALINSVNGEEN
EECCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
SIETILPLAKRYGAALIGLTLDDEGIPPTAAGRLAVARRLVERTEAMGIPRDDLMMDCLV
HHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LTVSAQPEGVPETLEAIRLVKQKLQITTSLGVSNVSFGLPNRVLINAAFFAMALNAGLDA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCE
AILNPQDQRMMETLRASAVLTGRDVRAEKYIAIEQQTHNGSKMETTGEQMKASTPLDILY
EEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCCCCHHHHH
QAVLSGDKDNIVSYINEALKTGLSPMELLDKALIPGIEEVGRRYGAGIYFLPQLMMAGET
HHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCHH
MTKSFERLRPELAKGPAKKAGTVVLATVKGDIHDIGKNIVSVMLANHGFEVIDLGKNVAN
HHHHHHHHCHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCCHHH
EEILRVAAEQKANIIGLSALMTTTMVHMQEIIKLVKEQGLNCKVMVGGAAVTEHYAREIG
HHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHC
ADGYAIDAVEAVNVAKKLAS
CCCCEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]